Abstract：
:Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Here we report the interesting phenomenon that puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length protein at the C-terminus. This was proved by using a carboxypeptidase digestion assay of the products obtained by Escherichia coli cell-free translation of human tau 4 repeat (tau4R) mRNA in the presence of low concentrations of puromycin or its derivatives. The tau4R mRNA was modified to code for three C-terminal methionines, which were radioactively labeled, followed by a stop codon. The translation products could not be digested by carboxy-peptidase if puromycin or a derivative was present at the C-terminus of full-length tau4R. Puromycin and its derivatives at 0. 04-1.0 microM bonded to 7-21% of full-length tau4R, depending on the ability to act as acceptor substrates. Furthermore, the bonding efficiency of a puromycin derivative to tau4R was decreased by addition of release factors. These results suggest that puromycin and its derivatives at concentrations lower than those able to compete effectively with aminoacyl-tRNA can bond specifically to full-length protein at a stop codon. This specific bonding of puromycin to full-length protein should be useful for in vitro selection of proteins and for in vitro and in vivo C-terminal end protein labeling.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Miyamoto-Sato E,Nemoto N,Kobayashi K,Yanagawa Hdoi
10.1093/nar/28.5.1176keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
1176-82issue
5eissn
0305-1048issn
1362-4962pii
gkd234journal_volume
28pub_type
杂志文章abstract：:A 30 nt RNA with a sequence designed to form an intramolecular triple helix was analyzed by one-and two-dimensional NMR spectroscopy and UV absorption measurements. NMR data show that the RNA contains seven pyrimidine-purine-pyrimidine base triples stabilized by Watson-Crick and Hoogsteen interactions. The temperature...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.14.2841
更新日期：1996-07-15 00:00:00
abstract：:We have determined the nucleotide sequence at the 3' end of the vesicular stomatitis virus (VSV) polymerase or L gene and compared it to that obtained from a defective interfering particle (DI) RNA generated by this virus. The latter (DI 0.50) contains a large internal deletion within this gene. The deletion begins ex...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.21.6919
更新日期：1982-11-11 00:00:00
abstract：:In this paper, we describe a new method for selecting RNA aptamers that cooperatively bind to two specific sites within a target RNA. We designed a selection system in which two RNAs, a target RNA and a RNA pool, were assembled by employing a pre-organized GAAA tetraloop-11-nt receptor interaction. This allows us to s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn1012
更新日期：2009-02-01 00:00:00
abstract：:The specific recognition of homopurine-homo pyrimidine regions in duplex DNA by triplex-forming oligonucleotides (TFOs) provides an attractive strategy for genetic manipulation. Alkylation of nucleobases with functionalized TFOs would have the potential for site-directed mutagenesis. Recently, we demonstrated that a T...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng031
更新日期：2003-03-15 00:00:00
abstract：:The human 7SL RNA component of the signal recognition particle can be separated into four major conformers by nondenaturing polyacrylamide gel electrophoresis. We have investigated what sequences give 7SL RNA the property to exist in different conformations. The human 7SL gene 7L30.1 was mutagenized using the random l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.11.4639
更新日期：1986-06-11 00:00:00
abstract：:We report thermodynamic values for binding of the guanosine nucleophile to the ribozyme derived from the Anabaena group I intron, and find that they are similar to those measured previously for the structurally distinct Tetrahymena ribozyme. The free energy of binding guanosine 5'-monophosphate (pG) at 30 degrees C is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.19.3722
更新日期：1996-10-01 00:00:00
abstract：:Toxicogenomics focuses on assessing the safety of compounds using gene expression profiles. Gene expression signatures from large toxicogenomics databases are expected to perform better than small databases in identifying biomarkers for the prediction and evaluation of drug safety based on a compound's toxicological m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku955
更新日期：2015-01-01 00:00:00
abstract：:The gene encoding DNA polymerase alpha from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amino acid sequence possesses the seven sequence motifs which characterise eukaryotic replicative DNA polymerases (I-VII) and four of five motifs (A-E) identified in alpha DNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.16.3643
更新日期：1993-08-11 00:00:00
abstract：:Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (posi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq341
更新日期：2010-09-01 00:00:00
abstract：:The SIGnaling Network Open Resource 2.0 (SIGNOR 2.0) is a public repository that stores signaling information as binary causal relationships between biological entities. The captured information is represented graphically as a signed directed graph. Each signaling relationship is associated to an effect (up/down-regul...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz949
更新日期：2020-01-08 00:00:00
abstract：:Interactive Tree Of Life (http://itol.embl.de) is a web-based tool for the display, manipulation and annotation of phylogenetic trees. It is freely available and open to everyone. In addition to classical tree viewer functions, iTOL offers many novel ways of annotating trees with various additional data. Current versi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr201
更新日期：2011-07-01 00:00:00
abstract：:Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.1.155
更新日期：1993-01-11 00:00:00
abstract：:Recombinant plasmids which contain EcoRI fragments of tobacco chloroplast DNA carrying tRNA genes were constructed. Plasmids pTC211 and pTC293 contain the base sequences for tRNAAsn in their 1.4 and 1.1 Md EcoRI fragments, respectively. These two tRNA sequences are identical and are; 5'-TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGC...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.21.5601
更新日期：1981-11-11 00:00:00
abstract：:Adaptation of eucaryotic cDNA to heterologous expression was studied by mutating the translation initiation (TI) region upstream (mTI) and downstream (MTI) of the start codon. In the mTI subregion the 8 bases flanking the invariant Shine-Dalgarno motif GG-AG were mutagenized exhaustively, while the MTI subregion was s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.12.3127
更新日期：1992-06-25 00:00:00
abstract：:A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finge...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.24.5223
更新日期：1994-12-11 00:00:00
abstract：:I have used a novel single-sided specific polymerase chain reaction (PCR) strategy inspired by ligation-mediated PCR to clone fragments of divergent homeobox genes from a flatworm, the planarian Polycelis nigra. Eight homeobox-containing fragments were amplified, belonging to the Hox, msh, NK-1 and NK-2 classes. Toget...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.8.1547
更新日期：1996-04-15 00:00:00
abstract：:We investigated the effects of the antiviral agent distamycin A and of a distamycin derivative (FCE 24517) which possesses antineoplastic activity on the binding of some regulatory proteins to DNA. Both compounds inhibited the binding to DNA of the ubiquitous octamer binding factor OTF 1 and of the erythroid specific ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.3.1051
更新日期：1989-02-11 00:00:00
abstract：:Use of antisense oligonucleotides is a versatile strategy for achieving control of gene expression. Unfortunately, the interpretation of antisense-induced phenotypes is sometimes difficult, and chemical modifications that improve the potency and specificity of antisense action would be useful. The introduction of lock...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf651
更新日期：2002-12-01 00:00:00
abstract：:The RosettaDesign server identifies low energy amino acid sequences for target protein structures (http://rosettadesign.med.unc.edu). The client provides the backbone coordinates of the target structure and specifies which residues to design. The server returns to the client the sequences, coordinates and energies of ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl163
更新日期：2006-07-01 00:00:00
abstract：:Upon growth transition of Escherichia coli cells from exponential to stationary phase, RNA polymerase is converted into at least three different forms (S1, S2 and S3), which could be separately isolated by phosphocellulose column chromatography (Ozaki et al., 1991 (2)). Here, the promoter selectivity of these three st...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.2.257
更新日期：1992-01-25 00:00:00
abstract：:In eukaryotic ribosome, the N domain of polypeptide release factor eRF1 is involved in decoding stop signals in mRNAs. However, structure of the decoding site remains obscure. Here, we specifically altered the stop codon recognition pattern of human eRF1 by point mutagenesis of the invariant Glu55 and Tyr125 residues ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki927
更新日期：2005-11-10 00:00:00
abstract：:The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce pADH06C. The yeast strains SX46A, 7799-4B and VV-6 are deficient in endogenous O6-alkylguanine-DNA-alkyltransferase and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.2.331
更新日期：1990-01-25 00:00:00
abstract：:The interplay between aromatic stacking and hydrogen bonding in nucleobases has been investigated via high-level quantum chemical calculations. The experimentally observed stacking arrangement between consecutive bases in DNA and RNA/DNA double helices is shown to enhance their hydrogen bonding ability as opposed to g...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki317
更新日期：2005-03-23 00:00:00
abstract：:NetMHC-3.0 is trained on a large number of quantitative peptide data using both affinity data from the Immune Epitope Database and Analysis Resource (IEDB) and elution data from SYFPEITHI. The method generates high-accuracy predictions of major histocompatibility complex (MHC): peptide binding. The predictions are bas...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn202
更新日期：2008-07-01 00:00:00
abstract：:Despite two decades of study, the full scope of RNAi in mammalian cells has remained obscure. Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis of gene expression changes and (iii) Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) using anti-AGO2 antibody to identif...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa478
更新日期：2020-07-27 00:00:00
abstract：:A feature of Haemophilus influenzae genomes is the presence of several loci containing tracts of six or more identical tetranucleotide repeat units. These repeat tracts are unstable and mediate high frequency, reversible alterations in the expression of surface antigens. This process, termed phase variation (PV), enab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki180
更新日期：2005-01-14 00:00:00
abstract：:An updated compilation of 300 E. coli mRNA promoter sequences is presented. For each sequence the most recent relevant paper was checked, to verify the location of the transcriptional start position as identified experimentally. We comment on the reliability of the sequence databanks and analyze the conservation of kn...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.7.1507
更新日期：1993-04-11 00:00:00
abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx500
更新日期：2017-08-21 00:00:00
abstract：:Near-UV difference spectral analysis of the triplex formed from d(C-T)6 and d(A-G)6.d(C-T)6 in neutral and acidic solution shows that the third strand dC residues are protonated at pH 7.0, far above their intrinsic pKa. Additional support for ion-dipole interactions between the third strand dC residues and the G.C tar...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.14.2692
更新日期：1995-07-25 00:00:00
abstract：:The agents daunomycin, ethidium bromide, distamycin A and cytochrome c inhibit DNA dependent DNA polymerase I (E. coli) reaction competitively to DNA. The influence of these template inactivators on the binding of DNA polymerase to native as well as denatured DNA has been determined by affinity chromatography. Cytochr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.1.63
更新日期：1974-01-01 00:00:00
