Abstract:
:The agents daunomycin, ethidium bromide, distamycin A and cytochrome c inhibit DNA dependent DNA polymerase I (E. coli) reaction competitively to DNA. The influence of these template inactivators on the binding of DNA polymerase to native as well as denatured DNA has been determined by affinity chromatography. Cytochrome c blocks the binding of the enzyme to double-stranded and to single-stranded DNA Sepharose. In contrast to these results daunomycin, ethidium bromide or distamycin A reduce the binding affinity only with denatured DNA Sepharose as matrix. These data are discussed with respect to the modification by template inactivators of the affinity of DNA to the different binding sites of the DNA polymerase.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Müller WE,Obermeier J,Totsuka A,Zahn RKdoi
10.1093/nar/1.1.63keywords:
subject
Has Abstractpub_date
1974-01-01 00:00:00pages
63-74issue
1eissn
0305-1048issn
1362-4962journal_volume
1pub_type
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