Abstract:
:Mammalian mRNAs are generated by complex and coordinated biogenesis pathways and acquire 5'-end m(7)G caps that play fundamental roles in processing and translation. Here we show that several selenoprotein mRNAs are not recognized efficiently by translation initiation factor eIF4E because they bear a hypermethylated cap. This cap modification is acquired via a 5'-end maturation pathway similar to that of the small nucle(ol)ar RNAs (sn- and snoRNAs). Our findings also establish that the trimethylguanosine synthase 1 (Tgs1) interacts with selenoprotein mRNAs for cap hypermethylation and that assembly chaperones and core proteins devoted to sn- and snoRNP maturation contribute to recruiting Tgs1 to selenoprotein mRNPs. We further demonstrate that the hypermethylated-capped selenoprotein mRNAs localize to the cytoplasm, are associated with polysomes and thus translated. Moreover, we found that the activity of Tgs1, but not of eIF4E, is required for the synthesis of the GPx1 selenoprotein in vivo.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wurth L,Gribling-Burrer AS,Verheggen C,Leichter M,Takeuchi A,Baudrey S,Martin F,Krol A,Bertrand E,Allmang Cdoi
10.1093/nar/gku580subject
Has Abstractpub_date
2014-07-01 00:00:00pages
8663-77issue
13eissn
0305-1048issn
1362-4962pii
gku580journal_volume
42pub_type
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