Abstract:
:The Genomes On Line Database (GOLD) is a web resource for comprehensive access to information regarding complete and ongoing genome sequencing projects worldwide. The database currently incorporates information on over 1500 sequencing projects, of which 294 have been completed and the data deposited in the public databases. GOLD v.2 has been expanded to provide information related to organism properties such as phenotype, ecotype and disease. Furthermore, project relevance and availability information is now included. GOLD is available at http://www.genomesonline.org. It is also mirrored at the Institute of Molecular Biology and Biotechnology, Crete, Greece at http://gold.imbb.forth.gr/
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Liolios K,Tavernarakis N,Hugenholtz P,Kyrpides NCdoi
10.1093/nar/gkj145keywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
D332-4issue
Database issueeissn
0305-1048issn
1362-4962pii
34/suppl_1/D332journal_volume
34pub_type
杂志文章abstract::Addition of thiol compounds containing an anionic group to the 3'-terminal unsaturated sugar of the 5' fragment obtained from an oligonucleotide containing an AP site cleaved by beta-elimination, can be followed by gel electrophoresis. The technique enables to distinguish between two mechanisms of cleavage of the C3'-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.20.9489
更新日期:1988-10-25 00:00:00
abstract::In 2.5 x 10(-4)M EDTA buffer, the derivative melting curve of calf thymus DNA shows a major band at 47 degrees with a shoulder at about 54 degrees . The fraction of melting area of this shoulder is about 13%. For reconstituted polylysine-calf thymus DNA complexes, in addition to the melting of free DNA regions at abou...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.2.257
更新日期:1974-02-01 00:00:00
abstract::The web software CHOP was developed to visualize the 'wobbling' in the third codon position of aligned DNA sequences. The simple features of this tool allow users to easily find regions suspected of containing coding sequences (CDSs). The program also allows visualization of the nucleotide diversity between two genomi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh448
更新日期:2004-07-01 00:00:00
abstract::Alterations in cancer genomes strongly influence clinical responses to treatment and in many instances are potent biomarkers for response to drugs. The Genomics of Drug Sensitivity in Cancer (GDSC) database (www.cancerRxgene.org) is the largest public resource for information on drug sensitivity in cancer cells and mo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1111
更新日期:2013-01-01 00:00:00
abstract::The 4.5S rRNA was isolated from the chloroplast ribosomes from Dryopteris acuminata. The complete nucleotide sequence was determined to be: OHUAAGGUCACGGCAAGACGAGCCGUUUAUCACCACGAUAGGUGCUAAGUGGAGGUGCAGUAAUGUAUGCAGCUGAGGC AUCCUAAUAGACCGAGAGGUUUGAACOH. The 4.5S rRNA is composed of 103 nucleotides and shows strong homolog...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.7.2257
更新日期:1982-04-10 00:00:00
abstract::The strand displacement activity of DNA polymerase δ is strongly stimulated by its interaction with proliferating cell nuclear antigen (PCNA). However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the polymerase to carry out strand displacement even in the absence of PCNA. We have examined in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv260
更新日期:2015-04-30 00:00:00
abstract::CSP41 is a ubiquitous chloroplast endoribonuclease belonging to the short chain dehydrogenase/reductase (SDR) superfamily. To help elucidate the role of CSP41 in chloroplast gene regulation, the mechanisms that determine its substrate recognition and catalytic activity were investigated. A divalent metal is required f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg640
更新日期:2003-08-01 00:00:00
abstract::Genome browsers are widely used for individually exploring various types of genomic data. A handful of genome browsers offer limited tools for collaboration among multiple users. Here, we describe PBrowse, an integrated real-time collaborative genome browser that enables multiple users to simultaneously view and acces...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1358
更新日期:2017-05-19 00:00:00
abstract::The Zebrafish Information Network (ZFIN) (https://zfin.org/) is the database for the model organism, zebrafish (Danio rerio). ZFIN expertly curates, organizes, and provides a wide array of zebrafish genetic and genomic data, including genes, alleles, transgenic lines, gene expression, gene function, mutant phenotypes,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1010
更新日期:2021-01-08 00:00:00
abstract::Translesion DNA synthesis (TLS) by the Y-family DNA polymerases Polη, Polι and Polκ, mediated via interaction with proliferating cell nuclear antigen (PCNA), is a crucial pathway that protects human cells against DNA damage. We report that Polη has three PCNA-interacting protein (PIP) boxes (PIP1, 2, 3) that contribut...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv712
更新日期:2015-09-18 00:00:00
abstract::We have created a database of comparatively derived group I intron secondary structure diagrams. This collection currently contains a broad sampling of phylogenetically and structurally similar and diverse structures from over 200 publicly available intron sequences. As more group I introns are sequenced and added to ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.17.3508
更新日期:1994-09-01 00:00:00
abstract::We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the degradosome complex and participates in mtRNA turnover and processing, while in Caenorhabditis elegans the hSUV3 orthologue is necessary for viability of early embryo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf647
更新日期:2002-12-01 00:00:00
abstract::Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.9.2085
更新日期:1980-05-10 00:00:00
abstract::Using a bio-oligo pull-down DNA-binding assay we investigated the binding capacity of endogenous, DNA damage-induced p53 in human diploid fibroblasts to several p53-responsive elements (REs) present in p53-regulated genes. During the course of p53 accumulation, we observed a decrease in p53 binding to the GADD45 but n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.11.2340
更新日期:2002-06-01 00:00:00
abstract::To elucidate the general constraints imposed on the structure of the D- and T-loops in functional tRNAs, active suppressor tRNAs were selected in vivo from a combinatorial tRNA gene library in which several nucleotide positions of these loops were randomized. Analysis of the nucleotide sequences of the selected clones...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg448
更新日期:2003-07-15 00:00:00
abstract::Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-le...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.22.5347
更新日期:1980-11-25 00:00:00
abstract::A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of oligonucleotide microarrays by generating readily hybridizable structure-free targets. Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn797
更新日期:2008-12-01 00:00:00
abstract::Aberrant CpG methylation changes occurring during tumour progression include the loss (hypomethylation) and gain (hypermethylation) of methyl groups. Techniques currently available for examining such changes either require selection of a region, then examination of methylation changes, or utilise methylation-sensitive...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.24.e123
更新日期:2001-12-15 00:00:00
abstract::The death of sympathetic neurons after nerve growth factor (NGF) withdrawal requires de novo gene expression. Dp5 was one of the first NGF withdrawal-induced genes to be identified and it encodes a proapoptotic BH3-only member of the Bcl-2 family. To study how dp5 transcription is regulated by NGF withdrawal we cloned...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp175
更新日期:2009-05-01 00:00:00
abstract::Mutational analysis has shown that the integrity of the region in domain III of 25S rRNA that is involved in binding of ribosomal protein L25 is essential for the production of mature 25S rRNA in the yeast Saccharomyces cerevisiae. However, even structural alterations that do not noticeably affect recognition by L25, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.24.5001
更新日期:2001-12-15 00:00:00
abstract::We have developed a new method for analysis of complex genetic systems by a combination of the Amplification Refractory Mutation System (ARMS) and Single-Strand Conformation Polymorphism (SSCP) analysis: ARMS-SSCP. Thus, a complex allelic series is subdivided into a number of groups by ARMS followed by the identificat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.5.1005
更新日期:1992-03-11 00:00:00
abstract::Therapeutic oligonucleotides are often modified using the phosphorothioate (PS) backbone modification which enhances stability from nuclease mediated degradation. However, substituting oxygen in the phosphodiester backbone with sulfur introduce chirality into the backbone such that a full PS 16-mer oligonucleotide is ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa031
更新日期:2020-02-28 00:00:00
abstract::Genome-wide association studies often incorporate information from public biological databases in order to provide a biological reference for interpreting the results. The dbSNP database is an extensive source of information on single nucleotide polymorphisms (SNPs) for many different organisms, including humans. We h...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1054
更新日期:2011-01-01 00:00:00
abstract::A screening for human proteins capable of interacting with influenza virus NS1 has been carried out using the two-hybrid genetic trap in yeast. A cDNA corresponding to the human homologue of Drosophila melanogaster Staufen protein (hStaufen) was isolated that fulfilled all genetic controls of the two-hybrid protocol. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.11.2241
更新日期:1999-06-01 00:00:00
abstract::Real-time PCR is being used increasingly as the method of choice for mRNA quantification, allowing rapid analysis of gene expression from low quantities of starting template. Despite a wide range of approaches, the same principles underlie all data analysis, with standard approaches broadly classified as either absolu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng073
更新日期:2003-07-15 00:00:00
abstract::PARP1 and PARP2 are implicated in the synthesis of poly(ADP-ribose) (PAR) after detection of DNA damage. The specificity of PARP1 and PARP2 interaction with long DNA fragments containing single- and/or double-strand breaks (SSBs and DSBs) have been studied using atomic force microscopy (AFM) imaging in combination wit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1476
更新日期:2016-04-07 00:00:00
abstract::The channel proteins belonging to the major intrinsic proteins (MIP) superfamily are diverse and are found in all forms of life. Water-transporting aquaporin and glycerol-specific aquaglyceroporin are the prototype members of the MIP superfamily. MIPs have also been shown to transport other neutral molecules and gases...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr914
更新日期:2012-01-01 00:00:00
abstract::The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation bet...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.23.4733
更新日期:1996-12-01 00:00:00
abstract::ApiDB (http://ApiDB.org) represents a unified entry point for the NIH-funded Apicomplexan Bioinformatics Resource Center (BRC) that integrates numerous database resources and multiple data types. The phylum Apicomplexa comprises numerous veterinary and medically important parasitic protozoa including human pathogenic ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl880
更新日期:2007-01-01 00:00:00
abstract::Transcriptomics (at the level of single cells, tissues and/or whole organisms) underpins many fields of biomedical science, from understanding the basic cellular function in model organisms, to the elucidation of the biological events that govern the development and progression of human diseases, and the exploration o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq667
更新日期:2010-09-01 00:00:00