Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA.

abstract：:Mechanisms that preserve genome integrity are highly important during the normal life cycle of human cells. Loss of genome protective mechanisms can lead to the development of diseases such as cancer. Checkpoint kinases function in the cellular surveillance pathways that help cells to cope with DNA damage. Importantly...

journal_title：Nucleic acids research

authors： Sørensen CS,Syljuåsen RG

更新日期：2012-01-01 00:00:00

abstract：:Previous studies have shown that (i) several RNAs induced by heat shock of Drosophila melanogaster cells are homologous to tandemly repeated alpha beta units found in cloned segments of D. melanogaster DNA, and (ii) the alpha beta sequences are present both at a major heat shock locus, 87Cl, and the chromocenter of po...

journal_title：Nucleic acids research

authors： Lis JT,Ish-Horowicz D,Pinchin SM

更新日期：1981-10-24 00:00:00

abstract：:Many microRNAs (miRNAs) are co-regulated during the same physiological process but the underlying cellular logic is often little understood. The conserved, immunomodulatory miRNAs miR-146 and miR-155, for instance, are co-induced in many cell types in response to microbial lipopolysaccharide (LPS) to feedback-repress ...

journal_title：Nucleic acids research

authors： Schulte LN,Westermann AJ,Vogel J

更新日期：2013-01-07 00:00:00

abstract：:The repetitive zinc finger domain of transcription factor IIIA binds 5S DNA and 5S RNA with similar affinity. Site directed mutagenesis of the Xenopus borealis somatic 5S RNA gene has been used to produce a series of derivatives of 5S RNA containing local sequence substitutions or sequence deletions. Gel mobility shif...

journal_title：Nucleic acids research

authors： Bogenhagen DF,Sands MS

更新日期：1992-06-11 00:00:00

abstract：:Genome-wide association studies (GWAS) have become a preferred method to identify new genetic susceptibility loci. This technique aims to understanding the molecular etiology of common diseases, but in many cases, it has led to the identification of loci with no obvious biological relevance. Herein, we show that previ...

journal_title：Nucleic acids research

authors： Galichon P,Mesnard L,Hertig A,Stengel B,Rondeau E

更新日期：2012-06-01 00:00:00

abstract：:The binding kinetics of NF-kappaB p50 to the Ig-kappaB site and to a DNA duplex with no specific binding site were determined under varying conditions of potassium chloride concentration using a surface plasmonresonance biosensor. Association and dissociation rate constants were measured enabling calculation of the di...

journal_title：Nucleic acids research

authors： Hart DJ,Speight RE,Cooper MA,Sutherland JD,Blackburn JM

更新日期：1999-02-15 00:00:00

abstract：:Invertebrate RNA viruses are targets of the host RNA interference (RNAi) pathway, which limits virus infection by degrading viral RNA substrates. Several insect RNA viruses encode suppressor proteins to counteract this antiviral response. We recently demonstrated that the dsDNA virus Invertebrate iridescent virus 6 (I...

journal_title：Nucleic acids research

authors： Bronkhorst AW,van Cleef KW,Venselaar H,van Rij RP

更新日期：2014-10-29 00:00:00

abstract：:The sequence GCGCGCGCGC contains three overlapping, mutually exclusive, BssHII restriction sites, each corresponding to one of the three different reading frames. When inserted into a plasmid and digested with BssHII, the three sites in this sequence are cleaved at an approximate ratio of 2:1:2. Consequently, this sys...

journal_title：Nucleic acids research

authors： Orum H,Poulsen LK

更新日期：1989-04-25 00:00:00

abstract：:REV1 and DNA Polymerase ζ (REV3 and REV7) play important roles in translesion DNA synthesis (TLS) in which DNA replication bypasses blocking lesions. REV1 and Polζ have also been implicated in promoting repair of DNA double-stranded breaks (DSBs). However, the mechanism by which these two TLS polymerases increase tole...

journal_title：Nucleic acids research

authors： Sharma S,Hicks JK,Chute CL,Brennan JR,Ahn JY,Glover TW,Canman CE

更新日期：2012-01-01 00:00:00

abstract：:We have analyzed the ATPase activity exhibited by the UvrABC DNA repair complex. The UvrA protein is an ATPase whose lack of DNA dependence may be related to the ATP induced monomer-dimer transitions. ATP induced dimerization may be responsible for the enhanced DNA binding activity observed in the presence of ATP. Alt...

journal_title：Nucleic acids research

authors： Oh EY,Claassen L,Thiagalingam S,Mazur S,Grossman L

更新日期：1989-06-12 00:00:00

abstract：:The growing application of gene expression profiling demands powerful yet user-friendly bioinformatics tools to support systems-level data understanding. NetworkAnalyst was first released in 2014 to address the key need for interpreting gene expression data within the context of protein-protein interaction (PPI) netwo...

journal_title：Nucleic acids research

authors： Zhou G,Soufan O,Ewald J,Hancock REW,Basu N,Xia J

更新日期：2019-07-02 00:00:00

abstract：:The leaf senescence database (LSD) is a comprehensive resource of senescence-associated genes (SAGs) and their corresponding mutants. Through manual curation and extensive annotation, we updated the LSD to a new version LSD 3.0, which contains 5853 genes and 617 mutants from 68 species. To provide sustainable and reli...

journal_title：Nucleic acids research

authors： Li Z,Zhang Y,Zou D,Zhao Y,Wang HL,Zhang Y,Xia X,Luo J,Guo H,Zhang Z

更新日期：2020-01-08 00:00:00

abstract：:Previous evidence indicates that telomeres resemble common fragile sites and present a challenge for DNA replication. The precise impediments to replication fork progression at telomeric TTAGGG repeats are unknown, but are proposed to include G-quadruplexes (G4) on the G-rich strand. Here we examined DNA synthesis and...

journal_title：Nucleic acids research

authors： Lormand JD,Buncher N,Murphy CT,Kaur P,Lee MY,Burgers P,Wang H,Kunkel TA,Opresko PL

更新日期：2013-12-01 00:00:00

abstract：:The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...

journal_title：Nucleic acids research

authors： Méjean V,Devedjian JC,Rives I,Alloing G,Claverys JP

更新日期：1991-10-25 00:00:00

abstract：:The interaction of several synthetic analogues of d-ApA with Poly U and Poly dT was examined to explore the effects of substituents at phosphorus on binding properties of oligonucleotides. These analogues contained a bulky, lipophilic group (2,2,2-trichloroethoxy or 2,2,2-trichloro-1,1-dimethylethoxy) a small, uncharg...

journal_title：Nucleic acids research

authors： Letsinger RL,Bach SA,Eadie JS

更新日期：1986-04-25 00:00:00

abstract：:The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...

journal_title：Nucleic acids research

authors： Ren Q,Au HH,Wang QS,Lee S,Jan E

更新日期：2014-08-01 00:00:00

abstract：:This report documents the error rate in a commercially distributed subset of the IMAGE Consortium mouse cDNA clone collection. After isolation of plasmid DNA from 1189 bacterial stock cultures, only 62. 2% were uncontaminated and contained cDNA inserts that had significant sequence identity to published data for the o...

journal_title：Nucleic acids research

authors： Halgren RG,Fielden MR,Fong CJ,Zacharewski TR

更新日期：2001-01-15 00:00:00

abstract：:DNA circuit is a versatile and highly-programmable toolbox which can potentially be used for the autonomous sensing of dynamic events, such as biomolecular interactions. However, the experimental implementation of in silico circuit designs has been hindered by the problem of circuit leakage. Here, we systematically an...

journal_title：Nucleic acids research

authors： Ang YS,Tong R,Yung LY

更新日期：2016-08-19 00:00:00

abstract：:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes an...

journal_title：Nucleic acids research

authors： Wang ML,Leitch AR,Schwarzacher T,Heslop-Harrison JS,Moore G

更新日期：1992-04-25 00:00:00

abstract：:A RNA interference (RNAi) like mechanism is involved in elimination of thousands of DNA segments from the developing somatic macronucleus of Tetrahymena, yet how specific internal eliminated sequences (IESs) are recognized remains to be fully elucidated. To define requirements for DNA rearrangement, we performed mutag...

journal_title：Nucleic acids research

authors： Kowalczyk CA,Anderson AM,Arce-Larreta M,Chalker DL

更新日期：2006-01-01 00:00:00

abstract：:We developed a framework for quick and reliable construction of complex gene circuits for genetically engineering mammalian cells. Our hierarchical framework is based on a novel nucleotide addressing system for defining the position of each part in an overall circuit. With this framework, we demonstrate construction o...

journal_title：Nucleic acids research

authors： Guye P,Li Y,Wroblewska L,Duportet X,Weiss R

更新日期：2013-09-01 00:00:00

abstract：:Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...

journal_title：Nucleic acids research

authors： Koropatnick J,Andrews G,Duerksen JD,Varshney U,Gedamu L

更新日期：1983-05-25 00:00:00

abstract：:For the isolation of single stranded plasmid DNA, various E. coli and E. coli-Streptomyces shuttle plasmids were equipped with the phage f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In...

journal_title：Nucleic acids research

authors： Hillemann D,Pühler A,Wohlleben W

更新日期：1991-02-25 00:00:00

abstract：:For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a heterologous mammalian ge...

journal_title：Nucleic acids research

authors： Adachi N,Ikeda H,Kikuchi A

更新日期：1994-10-11 00:00:00

abstract：:The National Center for Biotechnology Information (NCBI) Clone DB (http://www.ncbi.nlm.nih.gov/clone/) is an integrated resource providing information about and facilitating access to clones, which serve as valuable research reagents in many fields, including genome sequencing and variation analysis. Clone DB represen...

journal_title：Nucleic acids research

authors： Schneider VA,Chen HC,Clausen C,Meric PA,Zhou Z,Bouk N,Husain N,Maglott DR,Church DM

更新日期：2013-01-01 00:00:00

abstract：:Sgs1 is a member of the RecQ family of DNA helicases, which have been implicated in genomic stability, cancer and ageing. Srs2 is another DNA helicase that shares several phenotypic features with Sgs1 and double sgs1srs2 mutants have a severe synthetic growth phenotype. This suggests that there may be functional overl...

journal_title：Nucleic acids research

authors： Mankouri HW,Craig TJ,Morgan A

更新日期：2002-03-01 00:00:00

abstract：:We describe a new exonuclease-based method for joining and/or constructing two or more DNA molecules. DNA fragments containing ends complementary to those of a vector or another independent molecules were generated by the polymerase chain reaction. The 3' ends of these molecules as well as the vector DNA were then rec...

journal_title：Nucleic acids research

authors： Yang YS,Watson WJ,Tucker PW,Capra JD

更新日期：1993-04-25 00:00:00

abstract：:Proteins that catalyse homologous recombination have been identified in all living organisms and are essential for the repair of damaged DNA as well as for the generation of genetic diversity. In bacteria homologous recombination is performed by the RecA protein, whereas in the eukarya a related protein called Rad51 i...

journal_title：Nucleic acids research

authors： McIlwraith MJ,Hall DR,Stasiak AZ,Stasiak A,Wigley DB,West SC

更新日期：2001-11-15 00:00:00

abstract：:Various strategies employed in genomic DNA cloning by subtractive hybridization have been examined by computer simulations, with the comparison between the predictions and the published results. The result shows that the efficiency of target sequence enrichment and the sensitivity to experimental conditions depend str...

journal_title：Nucleic acids research

authors： Cho TJ,Park SS

更新日期：1998-03-15 00:00:00

abstract：:We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association w...

journal_title：Nucleic acids research

authors： Belikov SV,Belgovsky AI,Preobrazhenskaya OV,Karpov VL,Mirzabekov AD

更新日期：1993-02-25 00:00:00