A dsRNA-binding protein of a complex invertebrate DNA virus suppresses the Drosophila RNAi response.

abstract：:We have studied the role of the C-terminal domain of the alpha subunit (alphaCTD) of Escherichia coli RNA polymerase during transcription initiation at promoters lacking an UP-element. The temperature requirement for open complex formation was used as an indication of the kinetics of this process. We have previously s...

journal_title：Nucleic acids research

authors： Burns HD,Ishihama A,Minchin SD

更新日期：1999-05-01 00:00:00

abstract：:The rat beta-casein gene is a member of a small gene family, encoding the principal milk proteins. In order to understand the mechanisms by which its stage- and tissue-specific expression are regulated, initially, a 14 kb genomic clone containing the entire 7.5 kb rat beta-casein gene with 3.5 kb of 5' and 3.0 kb of 3...

journal_title：Nucleic acids research

authors： Lee KF,DeMayo FJ,Atiee SH,Rosen JM

更新日期：1988-02-11 00:00:00

abstract：:We describe here an energy based computer software suite for narrowing down the search space of tertiary structures of small globular proteins. The protocol comprises eight different computational modules that form an automated pipeline. It combines physics based potentials with biophysical filters to arrive at 10 pla...

journal_title：Nucleic acids research

authors： Jayaram B,Bhushan K,Shenoy SR,Narang P,Bose S,Agrawal P,Sahu D,Pandey V

更新日期：2006-01-01 00:00:00

abstract：:Release of phosphorothioate antisense oligonucleotides (PS-ASOs) from late endosomes (LEs) is a rate-limiting step and a poorly defined process for productive intracellular ASO drug delivery. Here, we examined the role of Golgi-endosome transport, specifically M6PR shuttling mediated by GCC2, in PS-ASO trafficking and...

journal_title：Nucleic acids research

authors： Liang XH,Sun H,Hsu CW,Nichols JG,Vickers TA,De Hoyos CL,Crooke ST

更新日期：2020-02-20 00:00:00

abstract：:KUPS (The University of Kansas Proteomics Service) provides high-quality protein-protein interaction (PPI) data for researchers developing and evaluating computational models for predicting PPIs by allowing users to construct ready-to-use data sets of interacting protein pairs (IPPs), non-interacting protein pairs (NI...

journal_title：Nucleic acids research

authors： Chen XW,Jeong JC,Dermyer P

更新日期：2011-01-01 00:00:00

abstract：:Long terminal repeat (LTR)-retrotransposons comprise a significant portion of the rice genome. Their complete characterization is thus necessary if the sequenced genome is to be annotated correctly. In addition, because LTR-retrotransposons can influence the expression of neighboring genes, the complete identification...

journal_title：Nucleic acids research

authors： Chaparro C,Guyot R,Zuccolo A,Piégu B,Panaud O

更新日期：2007-01-01 00:00:00

abstract：:Functional genomic elements are marked by characteristic DNA and histone modification signatures. How combinatorial chromatin modification states are recognized by epigenetic reader proteins and how this is linked to their biological function is largely unknown. Here we provide a detailed molecular analysis of chromat...

journal_title：Nucleic acids research

authors： Borgel J,Tyl M,Schiller K,Pusztai Z,Dooley CM,Deng W,Wooding C,White RJ,Warnecke T,Leonhardt H,Busch-Nentwich EM,Bartke T

更新日期：2017-02-17 00:00:00

abstract：:Analysis of the human VK (ref. 2) gene locus led to the detection of a new sequence family (L sequences). Its copy number is in the range of 10(2). The L sequences, which are about 500 bp long, are found as part of the 3' flanking regions of a clustered set of human VKI genes but they occur also separate from the gene...

journal_title：Nucleic acids research

authors： Straubinger B,Pech M,Mühlebach K,Jaenichen HR,Bauer HG,Zachau HG

更新日期：1984-07-11 00:00:00

abstract：:An alpha-neo-endorphin (alpha NE) gene, which we previously synthesized chemically and inserted into E. coli beta-galactosidase gene of pK013 plasmid, has been excised and fused to E. coli alkaline phosphatase (APase) gene. One of the transformants was named E15/pA alpha NE1. Under the APase gene regulation, APase-alp...

journal_title：Nucleic acids research

authors： Ohsuye K,Nomura M,Tanaka S,Kubota I,Nakazato H,Shinagawa H,Nakata A,Noguchi T

更新日期：1983-03-11 00:00:00

abstract：:Evidence has emerged that repair of clustered DNA lesions may be compromised, possibly leading to the formation of double-strand breaks (DSB) and, thus, to deleterious events. The first repair event occurring at a multiply damaged site (MDS) is of major importance and will largely contribute to the hazardousness of MD...

journal_title：Nucleic acids research

authors： Eot-Houllier G,Gonera M,Gasparutto D,Giustranti C,Sage E

更新日期：2007-01-01 00:00:00

abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...

journal_title：Nucleic acids research

authors： Wang XC,Wilson SC,Hammond MC

更新日期：2016-09-30 00:00:00

abstract：:A new thermodynamic database for normal and modified nucleic acids has been developed. This Thermodynamic Database for Nucleic Acids (NTDB) includes sequence, structure and thermodynamic information as well as experimental methods and conditions. In this release, there are 1851 sequences containing both normal and mod...

journal_title：Nucleic acids research

authors： Chiu WL,Sze CN,Ip LN,Chan SK,Au-Yeung SC

更新日期：2001-01-01 00:00:00

abstract：T:Activator protein 1 (AP-1) is a transcription factor that recognizes two versions of a 7-base pair response element, either 5΄- GAG CA-3΄ or 5΄- GAG CA-3΄ (where M = 5-methylcytosine). These two elements share the feature that 5-methylcytosine and thymine both have a methyl group in the same position, 5-carbon of the...

journal_title：Nucleic acids research

authors： Hong S,Wang D,Horton JR,Zhang X,Speck SH,Blumenthal RM,Cheng X

更新日期：2017-03-17 00:00:00

abstract：:In the known monoclinic crystals the 3-dimensional structure of the hexameric, replicative helicase RepA encoded by plasmid RSF1010 shows 6-fold rotational symmetry. In contrast, in the cubic crystal form at 2.55 A resolution described here RepA has 3-fold symmetry and consists of a trimer of dimers. To study structur...

journal_title：Nucleic acids research

authors： Ziegelin G,Niedenzu T,Lurz R,Saenger W,Lanka E

更新日期：2003-10-15 00:00:00

abstract：:About 40% of the genes in the nematode Caenorhabditis elegans have homologs in humans. Based on the history of this model system, it is clear that the application of genetic methods to the study of this set of genes would provide important clues to their function in humans. To facilitate such genetic studies, we are e...

journal_title：Nucleic acids research

authors： Edgley M,D'Souza A,Moulder G,McKay S,Shen B,Gilchrist E,Moerman D,Barstead R

更新日期：2002-06-15 00:00:00

abstract：:We describe here the cloning, characterization and expression in E. coli of the gene coding for a DNA methylase from Spiroplasma sp. strain MQ1 (M.SssI). This enzyme methylates completely and exclusively CpG sequences. The Spiroplasma gene was transcribed in E. coli using its own promoter. Translation of the entire me...

journal_title：Nucleic acids research

authors： Renbaum P,Abrahamove D,Fainsod A,Wilson GG,Rottem S,Razin A

更新日期：1990-03-11 00:00:00

abstract：:Enzymes of intermediary metabolism are often reported to have moonlighting functions as RNA-binding proteins and have regulatory roles beyond their primary activities. Human serine hydroxymethyltransferase (SHMT) is essential for the one-carbon metabolism, which sustains growth and proliferation in normal and tumour c...

journal_title：Nucleic acids research

authors： Guiducci G,Paone A,Tramonti A,Giardina G,Rinaldo S,Bouzidi A,Magnifico MC,Marani M,Menendez JA,Fatica A,Macone A,Armaos A,Tartaglia GG,Contestabile R,Paiardini A,Cutruzzolà F

更新日期：2019-05-07 00:00:00

abstract：:The log kobs vs. pH profiles were determined in the intermediate acidity region for the glycosyl hydrolysis of guanosine and its 8-amino, 8-monomethylamino, 8-dimethylamino and 8-bromo derivatives. The decreased rate of the 8-amino and enhanced rate of the 8-bromo compound compared to guanosine support an A type mecha...

journal_title：Nucleic acids research

authors： Jordan F,Niv H

更新日期：1977-03-01 00:00:00

abstract：:A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...

journal_title：Nucleic acids research

authors： Karataev GI,Permogorov VI,Vologodskii AV,Frank-Kamenetskii MD

更新日期：1978-07-01 00:00:00

abstract：:A list of DNA sequences cloned from the human genome is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic clones comprising gene and pseudogene sequences, uncharacterised DNA segments and repetitive DNA elements. ...

journal_title：Nucleic acids research

authors： Schmidtke J,Cooper DN

更新日期：1988-01-01 00:00:00

abstract：:Photoalkylation reactions with 2-propanol, initiated with di-tert-butyl peroxide, of a variety of purine and pyrimidine mononucleotides and dinucleoside monophosphates lead to the substitution of an alpha-hydroxyisopropyl group for the H-8 atom of adenosine and the addition of the alcohol across the 5,6-double bond of...

journal_title：Nucleic acids research

authors： Havron A,Sperling J,Elad D

更新日期：1976-07-01 00:00:00

abstract：:DNA3'pp5'G caps synthesized by the 3'-PO4/5'-OH ligase RtcB have a strong impact on enzymatic reactions at DNA 3'-OH ends. Aprataxin, an enzyme that repairs A5'pp5'DNA ends formed during abortive ligation by classic 3'-OH/5'-PO4 ligases, is also a DNA 3' de-capping enzyme, converting DNAppG to DNA3'p and GMP. By takin...

journal_title：Nucleic acids research

authors： Chauleau M,Jacewicz A,Shuman S

更新日期：2015-07-13 00:00:00

abstract：:The biogenesis of ribosomes in vivo is an essential process for cellular functions. Transcription of ribosomal RNA (rRNA) genes is the rate-limiting step in ribosome biogenesis controlled by environmental conditions. Here, we investigated the role of folate antagonist on changes of DNA double-strand breaks (DSBs) land...

journal_title：Nucleic acids research

authors： Xie Q,Li C,Song X,Wu L,Jiang Q,Qiu Z,Cao H,Yu K,Wan C,Li J,Yang F,Huang Z,Niu B,Jiang Z,Zhang T

更新日期：2017-03-17 00:00:00

abstract：:The DNA-binding protein RdgC has been identified as an inhibitor of RecA-mediated homologous recombination in Escherichia coli. In Neisseria species, RdgC also has a role in virulence-associated antigenic variation. We have previously solved the crystal structure of the E. coli RdgC protein and shown it to form a toro...

journal_title：Nucleic acids research

authors： Briggs GS,Yu J,Mahdi AA,Lloyd RG

更新日期：2010-10-01 00:00:00

abstract：:The Fos and Jun family of transcription factors contain an invariant sequence motif lysine-cysteine-arginine (KCR) in the highly conserved DNA-binding region. Reduction of the cysteine residue is necessary to facilitate DNA-binding. Here, we examined the potential dual roles of the flanking lysine and arginine residue...

journal_title：Nucleic acids research

authors： Ng L,Forrest D,Curran T

更新日期：1993-12-25 00:00:00

abstract：:Unmodified tRNA molecules are useful for many purposes in cell-free protein biosynthesis, but there is little information about how the lack of tRNA post-transcriptional modifications affects the coding specificity for synonymous codons. In the present study, we prepared an unmodified form of Escherichia coli tRNA1Ser...

journal_title：Nucleic acids research

authors： Takai K,Takaku H,Yokoyama S

更新日期：1996-08-01 00:00:00

abstract：:Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we discover an HRP2-DPF3a-BAF epigenetic pathway that coordinates methylated histone H3 ly...

journal_title：Nucleic acids research

authors： Zhu X,Lan B,Yi X,He C,Dang L,Zhou X,Lu Y,Sun Y,Liu Z,Bai X,Zhang K,Li B,Li MJ,Chen Y,Zhang L

更新日期：2020-07-09 00:00:00

abstract：:As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...

journal_title：Nucleic acids research

authors： Yoshimura K,Rosenfeld MA,Nakamura H,Scherer EM,Pavirani A,Lecocq JP,Crystal RG

更新日期：1992-06-25 00:00:00

abstract：:The mini-exon, a short segment found at the 5' end of trypanosome mRNAs, is contributed by a small RNA, the mini-exon donor (medRNA). In vivo 32P-labeled medRNA, a set of smaller RNAs related to it, and mRNA, were purified from Trypanosoma brucei by hybrid selection and gel electrophoresis. Using RNA fingerprinting an...

journal_title：Nucleic acids research

authors： Freistadt MS,Cross GA,Branch AD,Robertson HD

更新日期：1987-12-10 00:00:00

abstract：:PCAF and hGCN5 are distinct human genes that encode proteins related to the yeast histone acetyltransferase and transcriptional adapter GCN5. The PCAF protein shares extensive similarity with the 439 amino acids of yGCN5, but it has an approximately 350 amino acid N-terminal extension that interacts with the transcrip...

journal_title：Nucleic acids research

authors： Smith ER,Belote JM,Schiltz RL,Yang XJ,Moore PA,Berger SL,Nakatani Y,Allis CD

更新日期：1998-06-15 00:00:00