Human CTF18-RFC clamp-loader complexed with non-synthesising DNA polymerase ε efficiently loads the PCNA sliding clamp.

abstract：:Bacillus subtilis OhrR is a dimeric repressor that senses organic peroxides and regulates the expression of the OhrA peroxiredoxin. Derepression results from oxidation of an active site cysteine which ultimately results in formation of a mixed disulfide with a low molecular weight thiol, a cyclic sulfenamide, or overo...

journal_title：Nucleic acids research

authors： Eiamphungporn W,Soonsanga S,Lee JW,Helmann JD

更新日期：2009-03-01 00:00:00

abstract：:Various strategies employed in genomic DNA cloning by subtractive hybridization have been examined by computer simulations, with the comparison between the predictions and the published results. The result shows that the efficiency of target sequence enrichment and the sensitivity to experimental conditions depend str...

journal_title：Nucleic acids research

authors： Cho TJ,Park SS

更新日期：1998-03-15 00:00:00

abstract：:YY1 is ubiquitously expressed zinc finger DNA binding protein. It can act as a transcriptional repressor or activator and, when binding at the initiator element, as a component of the basal transcription complex. Binding sites for YY1 have been reported in a wide variety of promoters and they exhibit substantial diver...

journal_title：Nucleic acids research

authors： Hyde-DeRuyscher RP,Jennings E,Shenk T

更新日期：1995-11-11 00:00:00

abstract：:The GeneNet system is designed for collection and analysis of the data on gene and metabolic networks, signal transduction pathways and kinetic characteristics of elementary processes. In the past 2 years, the GeneNet structure was considerably improved: (i) the current version of the database is now implemented using...

journal_title：Nucleic acids research

authors： Ananko EA,Podkolodny NL,Stepanenko IL,Podkolodnaya OA,Rasskazov DA,Miginsky DS,Likhoshvai VA,Ratushny AV,Podkolodnaya NN,Kolchanov NA

更新日期：2005-01-01 00:00:00

abstract：:A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finge...

journal_title：Nucleic acids research

authors： Krämer A,Legrain P,Mulhauser F,Gröning K,Brosi R,Bilbe G

更新日期：1994-12-11 00:00:00

abstract：:This work presents a neutral filter elution method for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay will detect the number of double strand breaks induced by as little as 1000 rad of X-ray. The rate of DNA elution through the filters under neutral conditions increases with X-ray dose....

journal_title：Nucleic acids research

authors： Bradley MO,Kohn KW

更新日期：1979-10-10 00:00:00

abstract：:Alternative RNA splicing is an essential process to yield proteomic diversity in eukaryotic cells, and aberrant splicing is often associated with numerous human diseases and cancers. We recently described serine/arginine-rich splicing factor 3 (SRSF3 or SRp20) being a proto-oncogene. However, the SRSF3-regulated splic...

journal_title：Nucleic acids research

authors： Ajiro M,Jia R,Yang Y,Zhu J,Zheng ZM

更新日期：2016-02-29 00:00:00

abstract：:Human Protein Reference Database (HPRD) (http://www.hprd.org) was developed to serve as a comprehensive collection of protein features, post-translational modifications (PTMs) and protein-protein interactions. Since the original report, this database has increased to >20 000 proteins entries and has become the largest...

journal_title：Nucleic acids research

authors： Mishra GR,Suresh M,Kumaran K,Kannabiran N,Suresh S,Bala P,Shivakumar K,Anuradha N,Reddy R,Raghavan TM,Menon S,Hanumanthu G,Gupta M,Upendran S,Gupta S,Mahesh M,Jacob B,Mathew P,Chatterjee P,Arun KS,Sharma S,Chand

更新日期：2006-01-01 00:00:00

abstract：:The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction during transposition. In this study, comparative DNA-binding analyses were performed using purified full-length Tnp and a C-terminal deletion variant (delta369) that lacks the putative dimerization domain. The shape of t...

journal_title：Nucleic acids research

authors： York D,Reznikoff WS

更新日期：1996-10-01 00:00:00

abstract：:By subtractive screening of a library made from mRNA of lipopolysaccharide (LPS)-stimulated mouse B lymphocytes we isolated cDNA-clones encoding the ribosomal protein L7. Human L7 mRNA was cloned from activated T-lymphocytes. Although no specific function of L7 in the translation apparatus is known as yet, it should b...

journal_title：Nucleic acids research

authors： Hemmerich P,von Mikecz A,Neumann F,Sözeri O,Wolff-Vorbeck G,Zoebelein R,Krawinkel U

更新日期：1993-01-25 00:00:00

abstract：:The Transcription Regulatory Regions Database (TRRD) is a curated database designed for accumulation of experimental data on extended regulatory regions of eukaryotic genes, the regulatory elements they contain, i.e., transcription factor binding sites, promoters, enhancers, silencers, etc., and expression patterns of...

journal_title：Nucleic acids research

authors： Kolchanov NA,Ananko EA,Podkolodnaya OA,Ignatieva EV,Stepanenko IL,Kel-Margoulis OV,Kel AE,Merkulova TI,Goryachkovskaya TN,Busygina TV,Kolpakov FA,Podkolodny NL,Naumochkin AN,Romashchenko AG

更新日期：1999-01-01 00:00:00

abstract：:Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are importan...

journal_title：Nucleic acids research

authors： Haac ME,Anderson MA,Eggleston H,Myles KM,Adelman ZN

更新日期：2015-04-20 00:00:00

abstract：:Resonances of the water exchangeable iminoprotons of the acceptor and D stem of yeast tRNAPhe have been assigned by means of Nuclear Overhauser Effects (NOE's). Assignments were made for spectra recorded from tRNA dialysed against a buffer with 110 mM sodium and 5 mM magnesium ions and against a buffer with 430 mM sod...

journal_title：Nucleic acids research

authors： Heerschap A,Haasnoot CA,Hilbers CW

更新日期：1982-11-11 00:00:00

abstract：:Overlap-directed DNA assembly methods allow multiple DNA parts to be assembled together in one reaction. These methods, which rely on sequence homology between the ends of DNA parts, have become widely adopted in synthetic biology, despite being incompatible with a key principle of engineering: modularity. To answer t...

journal_title：Nucleic acids research

authors： Casini A,MacDonald JT,De Jonghe J,Christodoulou G,Freemont PS,Baldwin GS,Ellis T

更新日期：2014-01-01 00:00:00

abstract：:The Mouse Genome Database (MGD; http://www.informatics.jax.org) is the primary community model organism database for the laboratory mouse and serves as the source for key biological reference data related to mouse genes, gene functions, phenotypes and disease models with a strong emphasis on the relationship of these ...

journal_title：Nucleic acids research

authors： Bult CJ,Eppig JT,Blake JA,Kadin JA,Richardson JE,Mouse Genome Database Group.

更新日期：2016-01-04 00:00:00

abstract：:Cyclophilins are a family of proteins that share a common, highly conserved sequence motif. Cyclophilins bind transiently to other proteins and facilitate their folding. One member of the family, hCypH, is part of the human spliceosomal [U4/U6.U5] tri-snRNP complex; it associates specifically and stably with the U4/U6...

journal_title：Nucleic acids research

authors： Ingelfinger D,Göthel SF,Marahiel MA,Reidt U,Ficner R,Lührmann R,Achsel T

更新日期：2003-08-15 00:00:00

abstract：:To study the role of the poly(A) tail length during the replication of poly(A)-containing plus-strand RNA virus, we have developed a simple reverse transcription polymerase chain reaction (RT-PCR)-based method that substantially improves the previously reported PAT [poly(A) test] assay. In contrast to the PAT assay, t...

journal_title：Nucleic acids research

authors： Kusov YY,Shatirishvili G,Dzagurov G,Gauss-Müller V

更新日期：2001-06-15 00:00:00

abstract：:Six 1',5'-anhydrohexitol uridine triphosphates were synthesized with aromatic substitutions appended via a carboxamide linker to the 5-position of their bases. An improved method for obtaining such 5-substituted hexitol nucleosides and nucleotides is described. The incorporation profile of the nucleotide analogues int...

journal_title：Nucleic acids research

authors： Renders M,Dumbre S,Abramov M,Kestemont D,Margamuljana L,Largy E,Cozens C,Vandenameele J,Pinheiro VB,Toye D,Frère JM,Herdewijn P

更新日期：2019-03-18 00:00:00

abstract：:Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization...

journal_title：Nucleic acids research

authors： Uno N,Kaku N,Morinaga Y,Hasegawa H,Yanagihara K

更新日期：2020-09-04 00:00:00

abstract：:Not I linking clones contain sequences flanking Not I recognition sites and were previously shown to be tightly associated with CpG islands and genes. To directly assess the value of Not I clones in genome research, high density grids with 50 000 Not I linking clones originating from six representative Not I linking l...

journal_title：Nucleic acids research

authors： Zabarovsky ER,Gizatullin R,Podowski RM,Zabarovska VV,Xie L,Muravenko OV,Kozyrev S,Petrenko L,Skobeleva N,Li J,Protopopov A,Kashuba V,Ernberg I,Winberg G,Wahlestedt C

更新日期：2000-04-01 00:00:00

abstract：:Protein classification by machine learning algorithms is now widely used in structural and functional annotation of proteins. The Protein Classification Benchmark collection (http://hydra.icgeb.trieste.it/benchmark) was created in order to provide standard datasets on which the performance of machine learning methods ...

journal_title：Nucleic acids research

authors： Sonego P,Pacurar M,Dhir S,Kertész-Farkas A,Kocsor A,Gáspári Z,Leunissen JA,Pongor S

更新日期：2007-01-01 00:00:00

abstract：:Anti-silencing function 1 (Asf1) and Chromatin Assembly Factor 1 (CAF-1) chaperone histones H3/H4 during the assembly of nucleosomes on newly replicated DNA. To understand the mechanism of histone H3/H4 transfer among Asf1, CAF-1 and DNA from a thermodynamic perspective, we developed and employed biophysical approache...

journal_title：Nucleic acids research

authors： Liu WH,Roemer SC,Port AM,Churchill ME

更新日期：2012-12-01 00:00:00

abstract：:Cancer-associated somatic mutations outside protein-coding regions remain largely unexplored. Analyses of the TERT locus have indicated that non-coding regulatory mutations can be more frequent than previously suspected and play important roles in oncogenesis. Using a computational method called SASE-hunter, developed...

journal_title：Nucleic acids research

authors： Smith KS,Yadav VK,Pedersen BS,Shaknovich R,Geraci MW,Pollard KS,De S

更新日期：2015-06-23 00:00:00

abstract：:We have isolated the 5'-end of the gene for the rat and human link protein by screening genomic libraries with oligonucleotides corresponding to the 5'-cDNA sequence. Several overlapping clones were isolated for the human link protein gene, while only one clone was obtained for the rat. All the clones contained a sing...

journal_title：Nucleic acids research

authors： Rhodes C,Savagner P,Line S,Sasaki M,Chirigos M,Doege K,Yamada Y

更新日期：1991-04-25 00:00:00

abstract：:Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Here we report the interesting phenomenon that puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length ...

journal_title：Nucleic acids research

authors： Miyamoto-Sato E,Nemoto N,Kobayashi K,Yanagawa H

更新日期：2000-03-01 00:00:00

abstract：:A fraction of the ribosomal 28S genes in some insects are interrupted at specific sites by insertion elements R1 and R2 (also called Type I and II). These elements contain long open-reading frames with homology to reverse transcriptase. We have identified in the silkmoth, Bombyx mori, copies of these elements which ha...

journal_title：Nucleic acids research

authors： Xiong Y,Burke WD,Jakubczak JL,Eickbush TH

更新日期：1988-11-25 00:00:00

abstract：:Eukaryotic transcription factor B (TFB) proteins are homologous to KsgA/Dim1 ribosomal RNA (rRNA) methyltransferases. The mammalian TFB1, mitochondrial (TFB1M) factor is an essential protein necessary for mitochondrial gene expression. TFB1M mediates an rRNA modification in the small ribosomal subunit and thus plays a...

journal_title：Nucleic acids research

authors： Guja KE,Venkataraman K,Yakubovskaya E,Shi H,Mejia E,Hambardjieva E,Karzai AW,Garcia-Diaz M

更新日期：2013-09-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) is entirely dependent on nuclear genes for its transcription and replication. One of these genes is TOP1MT, which encodes the mitochondrial DNA topoisomerase IB, involved in mtDNA relaxation. To elucidate TOP1MT regulation, we performed genome-wide profiling across the 60-cell line panel (the...

journal_title：Nucleic acids research

authors： Zoppoli G,Douarre C,Dalla Rosa I,Liu H,Reinhold W,Pommier Y

更新日期：2011-08-01 00:00:00

abstract：:Fibroblast growth factor receptor 3 (FGFR3) plays an important role in cartilage development. Although upregulation of FGFR3 expression in response to bone morphogenetic protein-2 (BMP-2) has been reported, the molecular mechanisms remain unknown. In this study, we used in vivo approaches to characterize BMP-2-induced...

journal_title：Nucleic acids research

authors： Sun F,Chen Q,Yang S,Pan Q,Ma J,Wan Y,Chang CH,Hong A

更新日期：2009-07-01 00:00:00

abstract：:Benzo[a]pyrene is an important environmental mutagen and carcinogen. Its metabolism in cells yields the mutagenic, key ultimate carcinogen 7R,8S,9S,10R-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide, (+)-anti-BPDE, which reacts via its 10-position with N2-dG in DNA to form the adduct (+)-trans-anti-BPDE-N2-dG. To ga...

journal_title：Nucleic acids research

authors： Zhao B,Wang J,Geacintov NE,Wang Z

更新日期：2006-01-13 00:00:00