Abstract：
:Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization, flow cytometric detection of specific genomic DNA sequences has not been established. Here, we present a flow cytometry assay for detection of single-copy genomic sequences in human lymphocytes using in situ PCR with universal energy transfer-labelled primers.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Uno N,Kaku N,Morinaga Y,Hasegawa H,Yanagihara Kdoi
10.1093/nar/gkaa515subject
Has Abstractpub_date
2020-09-04 00:00:00pages
e86issue
15eissn
0305-1048issn
1362-4962pii
5858117journal_volume
48pub_type
杂志文章abstract：:Synthesis of (p)ppRNA-DNA chains by purified HeLa cell DNA primase-DNA polymerase alpha (pol alpha-primase) was compared with those synthesized by a multiprotein form of DNA polymerase alpha (pol alpha 2) using unique single-stranded DNA templates containing the origin of replication for simian virus 40 (SV40) DNA. Th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.18.7305
更新日期：1986-09-25 00:00:00
abstract：:Unrepaired DNA damage may arrest ongoing replication forks, potentially resulting in fork collapse, increased mutagenesis and genomic instability. Replication through DNA lesions depends on mono- and polyubiquitylation of proliferating cell nuclear antigen (PCNA), which enable translesion synthesis (TLS) and template ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks850
更新日期：2012-11-01 00:00:00
abstract：:In Arabidopsis thaliana, endogenous trans-acting and exogenous siRNA pathways are initiated by the interaction of DRB4 with trigger dsRNA. Further, DCL4:DRB4 complex cleaves the dsRNA into 21 bp siRNA. Understanding molecular determinants and mechanistic details of dsRNA recognition by DRB4 is vital for inducing long-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx481
更新日期：2017-08-21 00:00:00
abstract：:The complete mitochondrial (mt) genome of Crossostoma lacustre, a freshwater loach from mountain stream of Taiwan, has been cloned and sequenced. This fish mt genome, consisting of 16558 base-pairs, encodes genes for 13 proteins, two rRNAs, and 22 tRNAs, in addition to a regulatory sequence for replication and transcr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.18.4853
更新日期：1992-09-25 00:00:00
abstract：:Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.13.2806
更新日期：1999-07-01 00:00:00
abstract：:The history and mechanism of molecular evolution in DNA have been greatly elucidated by contributions from genetics, probability theory and bioinformatics--indeed, mathematical developments such as Kimura's neutral theory, Kingman's coalescent theory and efficient software such as BLAST, ClustalW, Phylip, etc., provid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp477
更新日期：2009-07-01 00:00:00
abstract：:We report the extended database and data mining resources newly released in the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). H-InvDB is a comprehensive annotation resource of human genes and transcripts, and consists of two main views and six sub-databases. The latest release of H-InvDB (release 6...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1020
更新日期：2010-01-01 00:00:00
abstract：:OGG1 initiated base excision repair (BER) is the major pathway for repair of oxidative DNA base damage 8-oxoguanine (8-oxoG). Here, we report that RECQL4 DNA helicase, deficient in the cancer-prone and premature aging Rothmund-Thomson syndrome, physically and functionally interacts with OGG1. RECQL4 promotes catalytic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa392
更新日期：2020-07-09 00:00:00
abstract：:Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.22.6209
更新日期：1991-11-25 00:00:00
abstract：:A method is described to simultaneously amplify and sequence DNA using a new class of nucleotides containing boron. During the polymerase chain reaction, boron-modified nucleotides, i.e. 2'-deoxynucleoside 5'-alpha-[P-borano]-triphosphates, are incorporated into the product DNA. The boranophosphate linkages are resist...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.8.1611
更新日期：1997-04-15 00:00:00
abstract：:Adjustable transgene expression is considered key for next-generation molecular interventions in gene therapy scenarios, therapeutic reprogramming of clinical cell phenotypes for tissue engineering and sophisticated gene-function analyses in the post-genomic era. We have designed a portfolio of latest generation self-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh104
更新日期：2004-07-16 00:00:00
abstract：:FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.1.53
更新日期：1996-01-01 00:00:00
abstract：:A new class of synthetic DNA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the fo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.11.2413
更新日期：1980-06-11 00:00:00
abstract：:The generalized mismatch repair system of Streptococcus pneumoniae (the Hex system) can eliminate base pair mismatches arising in heteroduplex DNA during transformation or by DNA polymerase errors during replication. Mismatch repair is most likely initiated at nicks or gaps. The present work was started to examine the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.20.5525
更新日期：1991-10-25 00:00:00
abstract：:A coiled-coil microtubule-bundling protein, p180, was originally identified as one of the ribosome receptor candidates on the rough endoplasmic reticulum (ER) and is highly expressed in secretory tissues. Recently, we reported that p180 plays crucial roles in upregulating collagen biosynthesis, mainly by facilitating ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1197
更新日期：2012-04-01 00:00:00
abstract：:Pyrimidine adducts in cellular DNA arise from modification of the pyrimidine 5,6-double bond by oxidation, reduction or hydration. The biological outcome includes increased mutation rate and potential lethality. A major DNA N:-glycosylase responsible for the excision of modified pyrimidine bases is the base excision r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.3.604
更新日期：2001-02-01 00:00:00
abstract：:Diadenosine 5',5'"-P1,P4-tetraphosphate (Ap4A) has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 10(4)-10(6) times lower than that of the guanine analogue, Gp4G. The unexpectedly high level of Ap4A in dormant cysts of 2.37 pmol/10(6) cells can be reduced to 0.03 pmol/10(6) cells ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.3.1609
更新日期：1984-02-10 00:00:00
abstract：:Computational analysis of biosynthetic gene clusters (BGCs) has revolutionized natural product discovery by enabling the rapid investigation of secondary metabolic potential within microbial genome sequences. Grouping homologous BGCs into Gene Cluster Families (GCFs) facilitates mapping their architectural and taxonom...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa812
更新日期：2021-01-08 00:00:00
abstract：:Pluripotency, the ability of embryonic stem cells to differentiate into specialized cell types, is determined by ESC-specific gene regulators such as transcription factors and chromatin modification factors. It is not well understood how ESCs are poised for differentiation, however, and methods are needed for prognosi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx752
更新日期：2017-10-13 00:00:00
abstract：:M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)strand. The reaction...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.24.8765
更新日期：1985-12-20 00:00:00
abstract：:The nucleotide sequence of the faeD gene of Escherichia coli and the amino acid sequence of its product is presented. The faeD product is an outer membrane protein required for transport of K88ab fimbrial subunits across the outer membrane. The protein is synthesized as a precursor containing a signal peptide, and the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.6.2443
更新日期：1986-03-25 00:00:00
abstract：:The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids in solution. The goal is to predict not just a melting temperature for a hybridized pair of nucleic acids, but entire equilibrium melting profiles as a function of temperature. The two molecules are not required to be complementa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki591
更新日期：2005-07-01 00:00:00
abstract：:Non-structural protein 3 (NS3) of hepatitis C virus (HCV) has two distinct activities, protease and helicase, which are essential for HCV proliferation. In previous work, we obtained RNA aptamers (G9-I, II and III) which specifically bound the NS3 protease domain (DeltaNS3), efficiently inhibiting protease activity in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg291
更新日期：2003-04-01 00:00:00
abstract：:A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtaine...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.9.2103
更新日期：1993-05-11 00:00:00
abstract：:RaftProt (http://lipid-raft-database.di.uq.edu.au/) is a database of mammalian lipid raft-associated proteins as reported in high-throughput mass spectrometry studies. Lipid rafts are specialized membrane microdomains enriched in cholesterol and sphingolipids thought to act as dynamic signalling and sorting platforms....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1131
更新日期：2015-01-01 00:00:00
abstract：:Targeted differentiation of human induced pluripotent stem cells (hiPSCs) using only chemicals would have value-added clinical potential in the regeneration of complex cell types including cardiomyocytes. Despite the availability of several chemical inhibitors targeting proteins involved in signaling pathways, no bioa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx693
更新日期：2017-09-19 00:00:00
abstract：:The targeted adduction of aflatoxin B1- exo -8,9-epoxide (AFB1- exo -8,9-epoxide) to a specific guanine within an oligodeoxyribonucleotide containing multiple guanines was achieved using a DNA triplex to control sequence selectivity. The oligodeoxyribonucleotide d(AGAGAAGATTTTCTTCTCTTTTTTTTCTCTT), designated '3G', spo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.4.1070
更新日期：1998-02-15 00:00:00
abstract：:Starting from a synthetic E. coli promoter with the consensus sequences at -35 and -10 regions, a sequence CAT frequently occurred in the RNA start points of natural promoters was introduced in the downstream of the consensus sequences, and the sequences around the RNA start points as well as the relative positions of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.11.4085
更新日期：1985-06-11 00:00:00
abstract：:Restriction mapping demonstrated the presence of several distinct proviral forms of mouse mammary tumor virus in the genome of GR mice. One of these proviruses (GR-MTV-2) was highly amplified in GR 3A cells, a cell line derived from a GR mammary tumor. By the criterion of restriction mapping, the amplified GR-MTV-2 pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.23.5715
更新日期：1980-12-11 00:00:00
abstract：:MPromDb (Mammalian Promoter Database) is a curated database that strives to annotate gene promoters identified from ChIP-seq results with the goal of providing an integrated resource for mammalian transcriptional regulation and epigenetics. We analyzed 507 million uniquely aligned RNAP-II ChIP-seq reads from 26 differ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1171
更新日期：2011-01-01 00:00:00
