Abstract:
:Starting from a synthetic E. coli promoter with the consensus sequences at -35 and -10 regions, a sequence CAT frequently occurred in the RNA start points of natural promoters was introduced in the downstream of the consensus sequences, and the sequences around the RNA start points as well as the relative positions of CAT from the consensus sequences were altered. Analysis of the RNA start points and strength of these synthetic promoters in an in vitro transcription system provided evidence that the RNA start point was principally fixed by distance from the -10 consensus sequence (TATAAT). Neither the promoter strength nor the RNA start point was significantly influenced by the CAT sequence. The sequences around the RNA start points rather seemed to exert influence on the response of promoter to temperature and salts.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Aoyama T,Takanami Mdoi
10.1093/nar/13.11.4085subject
Has Abstractpub_date
1985-06-11 00:00:00pages
4085-96issue
11eissn
0305-1048issn
1362-4962journal_volume
13pub_type
杂志文章abstract::Plants have large diverse families of small secreted proteins (SSPs) that play critical roles in the processes of development, differentiation, defense, flowering, stress response, symbiosis, etc. Oryza sativa is one of the major crops worldwide and an excellent model for monocotyledonous plants. However, there had no...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2016-02-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1992-08-25 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1983-05-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-11-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
abstract::Ribosome recycling factor (RRF), elongation factor G (EF-G) and GTP split 70S ribosomes into subunits. Here, we demonstrated that the splitting was transient and the exhaustion of GTP resulted in re-association of the split subunits into 70S ribosomes unless IF3 (initiation factor 3) was present. However, the splittin...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-06-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku397
更新日期:2014-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2006-01-01 00:00:00
abstract::The recent publication of the Caenorhabditis elegans cisRED database has provided an extensive catalog of upstream elements that are conserved between nematode genomes. We have performed a secondary analysis to determine which subsequences of the cisRED motifs are found in multiple locations throughout the C. elegans ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
abstract::Sensory photoreceptors have enabled non-invasive and spatiotemporal control of numerous biological processes. Photoreceptor engineering has expanded the repertoire beyond natural receptors, but to date no generally applicable strategy exists towards constructing light-regulated protein actuators of arbitrary function....
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-11-16 00:00:00
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journal_title:Nucleic acids research
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更新日期:2003-08-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2014-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-09-15 00:00:00