Abstract:
:A 170bp long BamHI-Sau3A DNA fragment from the actIII-actI intergenic region of the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2) contains two promoters directing transcription in a divergent manner. One of them, the actIII promoter, is responsible for the transcription of the actIII gene and the other controls transcription of the adjacent actI region in the opposite direction. Weak activity of the actIII promoter can be detected in Streptomyces lividans and Bacillus subtilis in the absence but not in the presence of glucose. Neither promoter seems to function in Escherichia coli.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Parro V,Hopwood DA,Malpartida F,Mellado RPdoi
10.1093/nar/19.10.2623subject
Has Abstractpub_date
1991-05-25 00:00:00pages
2623-7issue
10eissn
0305-1048issn
1362-4962journal_volume
19pub_type
杂志文章abstract::Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotid...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.12.4391
更新日期:1977-12-01 00:00:00
abstract::G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.7.1613
更新日期:1998-04-01 00:00:00
abstract::The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.24.6805
更新日期:1991-12-25 00:00:00
abstract::Here we examined the role of cellular vitamin C in genotoxicity of carcinogenic chromium(VI) that requires reduction to induce DNA damage. In the presence of ascorbate (Asc), low 0.2-2 microM doses of Cr(VI) caused 10-15 times more chromosomal breakage in primary human bronchial epithelial cells or lung fibroblasts. D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1069
更新日期:2007-01-01 00:00:00
abstract::The Arabidopsis Information Resource (TAIR, http://arabidopsis.org) is the model organism database for the fully sequenced and intensively studied model plant Arabidopsis thaliana. Data in TAIR is derived in large part from manual curation of the Arabidopsis research literature and direct submissions from the research...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm965
更新日期:2008-01-01 00:00:00
abstract::Sgs1 is a member of the RecQ family of DNA helicases, which have been implicated in genomic stability, cancer and ageing. Srs2 is another DNA helicase that shares several phenotypic features with Sgs1 and double sgs1srs2 mutants have a severe synthetic growth phenotype. This suggests that there may be functional overl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.5.1103
更新日期:2002-03-01 00:00:00
abstract::We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.20.7515
更新日期:1985-10-25 00:00:00
abstract::Telomeres comprise the protective caps of natural chromosome ends and function in the suppression of DNA damage signaling and cellular senescence. Therefore, techniques used to determine telomere length are important in a number of studies, ranging from those investigating telomeric structure to effects on human disea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1315
更新日期:2014-02-01 00:00:00
abstract::Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1149
更新日期:2020-12-16 00:00:00
abstract::The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku622
更新日期:2014-08-01 00:00:00
abstract::IS200 is found throughout Enterobacteriaceae and transposes at a notoriously low frequency. In addition to the transposase protein (TnpA), IS200 encodes an uncharacterized Hfq-binding sRNA that is encoded opposite to the tnpA 5'UTR. In the current work we asked if this sRNA represses tnpA expression. We show here that...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv584
更新日期:2015-07-27 00:00:00
abstract::A tetradecanucleotide U-A-G-C(U-C-G)2G-G-C-Up corresponding to bases 21-34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of chemically synthesized oligonucleotides: 1) U-A-G-C + U-C-G-U-C-G + G-G-C-Up and 2) U-A-G-C + U-C-G-U + C-G-G-G-C-Up. In reactio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.17.3909
更新日期:1980-09-11 00:00:00
abstract::Eukaryotic elongation factor 1 A (eEF1A, formerly elongation factor-1 alpha) is an important component of the protein synthesis apparatus. Here we report the isolation and characterization of the cDNA sequence encoding rabbit eEF1A-2, an isoform of eEF1A, as well as a structural and functional comparison of the two ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.8.1884
更新日期:1998-04-15 00:00:00
abstract::Accurate mapping of spliced RNA-Seq reads to genomic DNA has been known as a challenging problem. Despite significant efforts invested in developing efficient algorithms, with the human genome as a primary focus, the best solution is still not known. A recently introduced tool, TrueSight, has demonstrated better perfo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1141
更新日期:2014-02-01 00:00:00
abstract::IGF2 mRNA-binding protein 1 (IMP1) is a key regulator of messenger RNA (mRNA) metabolism and transport in organismal development and, in cancer, its mis-regulation is an important component of tumour metastasis. IMP1 function relies on the recognition of a diverse set of mRNA targets that is mediated by the combinator...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz136
更新日期:2019-05-07 00:00:00
abstract::Plasmids containing a dihydrofolate reductase (DHFR) expression unit were transfected into DHFR-deficient Chinese hamster ovary (CHO) cells. Methotrexate exposure was used to select cells with amplified DHFR sequences. Three cell lines were isolated containing amplified copies of transfected DNA that had integrated in...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.4.1697
更新日期:1989-02-25 00:00:00
abstract::This laboratory has established in previous studies that Pmel 17, a gene expressed specifically in melanocytes, maps near the silver coat color locus (si/si) on mouse chromosome 10. In the current study, we have focused on determining whether or not the si allele carries a mutation in Pmel 17. Pmel 17 cDNA clones, iso...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.1.154
更新日期:1995-01-11 00:00:00
abstract::We have used temperature gradient gel electrophoresis (TGGE) to measure the progress of local denaturation in closed circular topoisomer DNA as a function of temperature and superhelicity (sigma). We describe the versatility of this method as a tool for detecting various conformational modifications of plasmid DNAs. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.11.e51
更新日期:2000-06-01 00:00:00
abstract::The activity of MHC class II promoters depends upon conserved regulatory signals one of which, the extended X-box, contains in its X2 subregion a sequence related to the cAMP response element, CRE and to the TPA response element, TRE. Accordingly, X2 is recognized by the AP-1 factor and by other c-Jun or c-Fos contain...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.8.1811
更新日期:1993-04-25 00:00:00
abstract::The DEAH-box NTPase Prp43 disassembles spliceosomes in co-operation with the cofactors Ntr1/Spp382 and Ntr2, forming the NTR complex. How Prp43 is regulated by its cofactors to discard selectively only intron-lariat spliceosomes (ILS) and defective spliceosomes and to prevent disassembly of earlier and properly assemb...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.15.6241
更新日期:1989-08-11 00:00:00
abstract::The sigma(54) factor associates with core RNA polymerase (RNAP) to form a holoenzyme that is unable to initiate transcription unless acted on by an activator protein. sigma(54) is closely involved in many steps of activator-dependent transcription, such as core RNAP binding, promoter recognition, activator interaction...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp419
更新日期:2009-07-01 00:00:00
abstract::The nucleotide sequences 3' to the translational termination codons in a collection of human genes have been analysed for evidence of a preferred 3' context for natural UAG codons. The aim was to see whether human UAG contexts can be related to the recent demonstration of the effects of 3' context on nonsense suppress...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.1.15
更新日期:1994-01-11 00:00:00
abstract::The expression pattern and regulatory functions of microRNAs (miRNAs) are intensively investigated in various tissues, cell types and disorders. Differential miRNA expression signatures have been revealed in healthy and unhealthy tissues using high-throughput profiling methods. For further analyses of miRNA signatures...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr214
更新日期:2011-07-01 00:00:00
abstract::E. coli genes for tRNAasp and tRNAtrp are transcribed by eukaryotic RNA polymerase III upon injection into nuclei of Xenopus oocytes. This indicates that some bacterial tRNA genes contain RNA polymerase III promoter sequences. We discuss evolutionary aspects of this finding. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.22.7153
更新日期:1982-11-25 00:00:00
abstract::Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90% decrease of tufB ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.22.9515
更新日期:1987-11-25 00:00:00
abstract::We have examined the interactions of UvrABC endonuclease with DNA containing the monoadducts of 8-methoxypsoralen (8-MOP) and 4,5',8-trimethylpsoralen (TMP). The UvrA and UvrB proteins were found to form a stable complex on DNA that contains the psoralen monoadducts. Subsequent binding of UvrC protein to this complex ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.12.4957
更新日期:1987-06-25 00:00:00
abstract::In conditions that mimic those of the living cell, where various biomolecules and other components are present, DNA strands can adopt many structures in addition to the canonical B-form duplex. Previous studies in the presence of cosolutes that induce molecular crowding showed that thermal stabilities of DNA structure...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1133
更新日期:2015-12-02 00:00:00
abstract::Residual dipolar coupling (RDC) represents one of the most exciting emerging NMR techniques for protein structure studies. However, solving a protein structure using RDC data alone is still a highly challenging problem. We report here a computer program, RDC-PROSPECT, for protein structure prediction based on a struct...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh204
更新日期:2004-01-26 00:00:00
abstract::With the rapid accumulation of our knowledge on diseases, disease-related genes and drug targets, network-based analysis plays an increasingly important role in systems biology, systems pharmacology and translational science. The new release of VisANT aims to provide new functions to facilitate the convenient network ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt401
更新日期:2013-07-01 00:00:00