Abstract：
:A tetradecanucleotide U-A-G-C(U-C-G)2G-G-C-Up corresponding to bases 21-34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of chemically synthesized oligonucleotides: 1) U-A-G-C + U-C-G-U-C-G + G-G-C-Up and 2) U-A-G-C + U-C-G-U + C-G-G-G-C-Up. In reaction 1) and the extent of joining *pG-G-C-Up to U-C-G-U-C-G was only 15.4% and the last ligation of the decamer to U-A-G-U proceeded to 27%. In reaction 2) joining between U-A-G-C and pU-C-G-Up gave a high yield (88%). The ligation of this octamer and *pC-G-G-G-C-Up also gave a satisfactory yield (52%). These reactions suggest that sequence preferences in RNA ligase reactions may arise from the structure of the 3'-end of acceptor molecules.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ohtsuka E,Doi T,Uemura H,Taniyama Y,Ikehara Mdoi
10.1093/nar/8.17.3909subject
Has Abstractpub_date
1980-09-11 00:00:00pages
3909-16issue
17eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract：:A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.19.4395
更新日期：1998-10-01 00:00:00
abstract：:Onconase, a cytotoxic ribonuclease from Rana pipiens, possesses pyroglutamate (Pyr) at the N-terminus and has a substrate preference for uridine-guanine (UG). To identify residues responsible for onconase's cytotoxicity, we cloned the rpr gene from genomic DNA and expressed it in Escherichia coli BL21(DE3). The recomb...
journal_title：Nucleic acids research
pub_type： 评论,杂志文章
doi：10.1093/nar/gkg746
更新日期：2003-09-15 00:00:00
abstract：:The HSV gene encoding ICP4 is negatively regulated and the HSV gene encoding thymidine kinase is positively regulated by ICP4 in vivo. We report that ICP4 is a component of a stable complex that contains protein and a sequence of approximately 28 nucleotides that span the ICP4 gene transcription initiation site. The a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.2.555
更新日期：1988-01-25 00:00:00
abstract：:Cells continuously monitor protein synthesis to prevent accumulation of aberrant polypeptides. Insufficient capacity of cellular degradative systems, chaperone shortage or high levels of mistranslation by ribosomes can result in proteotoxic stress and endanger proteostasis. One of the least explored reasons for mistra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa068
更新日期：2020-04-17 00:00:00
abstract：:Ribonuclease III of Escherichia coli is prominently involved in the endoribonucleolytic processing of cell and viral-encoded RNAs. Towards the goal of defining the RNA sequence and structural elements that establish specific catalytic cleavage of RNase III processing signals, this report demonstrates that a 60 nucleot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.8.1759
更新日期：1991-04-25 00:00:00
abstract：:The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considerin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1206
更新日期：2013-02-01 00:00:00
abstract：:The Bayesian regularization method for high-throughput differential analysis, described in Baldi and Long (A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes. Bioinformatics 2001: 17: 509-519) and implemented in the Cyber-T web server, is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks420
更新日期：2012-07-01 00:00:00
abstract：:Terminal deoxynucleotidyl transferase, which requires a single-stranded DNA primer under the usual assay conditions, can be made to accept double-stranded DNA as primer for the addition of either rNMP or dNMP, if Mg+2 ion is replaced by Co+2 ion. The priming efficiency in the presence of (C leads to) CO+2 ion with res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.1.101
更新日期：1976-01-01 00:00:00
abstract：:The activity of MHC class II promoters depends upon conserved regulatory signals one of which, the extended X-box, contains in its X2 subregion a sequence related to the cAMP response element, CRE and to the TPA response element, TRE. Accordingly, X2 is recognized by the AP-1 factor and by other c-Jun or c-Fos contain...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.8.1811
更新日期：1993-04-25 00:00:00
abstract：:We propose a novel mechanism of gene regulation in Mycobacterium tuberculosis where the protein Rv1222 inhibits transcription by anchoring RNA polymerase (RNAP) onto DNA. In contrast to our existing knowledge that transcriptional repressors function either by binding to DNA at specific sequences or by binding to RNAP,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv516
更新日期：2015-07-13 00:00:00
abstract：:Chromatin footprinting in Drosophila tissue culture cells has detected the binding of a non-histone protein at +8 of the distal Adh RNA start site, on a 10-bp direct repeat motif abutting a nucleosome positioned over the inactive Adh distal promoter. Alternatively the active promoter is bound by a transcription initia...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.17.4481
更新日期：1992-09-11 00:00:00
abstract：:The primary rRNA transcript contains a large external transcribed spacer (ETS) approximately 4,000 nucleotides in length. We have used subcloned DNA probes derived from the 5' end of the ETS in conjunction with Northern blot analysis of murine nuclear RNA to examine processing of this region. In agreement with the res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.18.7187
更新日期：1984-09-25 00:00:00
abstract：:Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.24.9965
更新日期：1986-12-22 00:00:00
abstract：:To prevent progressive telomere shortening as a result of conventional DNA replication, new telomeric DNA must be added onto the chromosome end. The de novo DNA synthesis involves elongation of the G-rich strand of the telomere by telomerase. In human cells, the CST complex (CTC1-STN1-TEN1) also functions in telomere ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx125
更新日期：2017-05-05 00:00:00
abstract：:Transposable elements (TEs) comprise a large proportion of long non-coding RNAs (lncRNAs). Here, we employed CRISPR to delete a short interspersed nuclear element (SINE) in Malat1, a cancer-associated lncRNA, to investigate its significance in cellular physiology. We show that Malat1 with a SINE deletion forms diffuse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1176
更新日期：2020-03-18 00:00:00
abstract：:Alu insertions have contributed to >11% of the human genome and ∼30-35 Alu subfamilies remain actively mobile, yet the characterization of polymorphic Alu insertions from short-read data remains a challenge. We build on existing computational methods to combine Alu detection and de novo assembly of WGS data as a means...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1089
更新日期：2015-12-02 00:00:00
abstract：:p53 as an effector of nucleolar stress is well defined, but p53 independent mechanisms are largely unknown. Like p53, the NF-κB transcription factor plays a critical role in maintaining cellular homeostasis under stress. Many stresses that stimulate NF-κB also disrupt nucleoli. However, the link between nucleolar func...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky455
更新日期：2018-07-06 00:00:00
abstract：:Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. He...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv614
更新日期：2015-10-30 00:00:00
abstract：:Efficient transcription from the human immunodeficiency virus (HIV) promoter depends on binding of the viral regulatory protein Tat to a cis-acting RNA regulatory element, TAR. Tat binds at a trinucleotide bulge located near the apex of the TAR stem-loop structure. An essential feature of Tat-TAR interaction is that t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.20.3974
更新日期：1996-10-15 00:00:00
abstract：:Quantitative real-time PCR (qPCR) is the method of choice for specific and sensitive quantification of nucleic acids. However, data validation is still a major issue, partially due to the complex effect of PCR inhibition on the results. If undetected PCR inhibition may severely impair the accuracy and sensitivity of r...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr778
更新日期：2012-02-01 00:00:00
abstract：:The compilation of 5S rRNA and 5S rRNA gene nucleotide sequences as of 30 September 1996, contains a total of 1661 primary structures of 5S rRNAs or their genes, which is an increase of 928 new sequence entries over the last compilation. It covers sequences from 54 archaea, 449 eubacteria, 34 plastids, nine mitochondr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.1.96
更新日期：1997-01-01 00:00:00
abstract：:Glucocorticoid (GC) signaling synchronizes the circadian rhythm of individual peripheral cells and induces the expression of circadian genes, including Period1 (Per1) and Period2 (Per2). However, no GC response element (GRE) has been reported in the Per2 promoter region. Here we report the molecular mechanisms of Per2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt307
更新日期：2013-07-01 00:00:00
abstract：:Alternative RNA splicing is an essential process to yield proteomic diversity in eukaryotic cells, and aberrant splicing is often associated with numerous human diseases and cancers. We recently described serine/arginine-rich splicing factor 3 (SRSF3 or SRp20) being a proto-oncogene. However, the SRSF3-regulated splic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1500
更新日期：2016-02-29 00:00:00
abstract：:The widely accepted model for the processing of tRNAs in Escherichia coli involves essential initial cleavages by RNase E within polycistronic transcripts to generate pre-tRNAs that subsequently become substrates for RNase P. However, recently we identified two polycistronic tRNA transcripts whose endonucleolytic proc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm991
更新日期：2008-02-01 00:00:00
abstract：:The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains sev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.9.3063
更新日期：1985-05-10 00:00:00
abstract：:Purified UvrA, UvrB, UvrC, UvrD, PolA and Lig proteins from Escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid DNA in an in vitro test system. The analysis of labeled reaction products on specific gel systems suggests that the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.24.10337
更新日期：1989-12-25 00:00:00
abstract：:DISCOVER1 (DIStribution COunter VERsion 1) is a new program that can identify DNA motifs occurring with a high deviation from the expected frequency. The program generates families of patterns, each family having a common set of defined bases. Undefined bases are inserted amongst the defined bases in different ways, t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.22.5152
更新日期：1993-11-11 00:00:00
abstract：:Loss-of-function in melanocortin 1 receptor (MC1R), a GS protein-coupled receptor that regulates signal transduction through cAMP and protein kinase A (PKA) in melanocytes, is a major inherited melanoma risk factor. Herein, we report a novel cAMP-mediated response for sensing and responding to UV-induced DNA damage re...
journal_title：Nucleic acids research
pub_type： 杂志文章,收录出版
doi：10.1093/nar/gkw871
更新日期：2016-12-15 00:00:00
abstract：:The repeating nucleotide sequence of 68 base pairs in the mtDNA from an ethidium-induced cytoplasmic petite mutant of yeast has been determined. For sequence analysis specifically primed and terminated RNA copies, obtained by in vitro transcription of the separated strands, were use. The sequence consists of 66 consec...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.7.2369
更新日期：1977-07-01 00:00:00
abstract：:Homeodomains appear to be one of the most frequently employed DNA-binding domains in a superfamily of transacting factors. It is likely that during evolution several sub-types of homeodomain have evolved from a common ancestral domain, resulting in distinct but closely related DNA-binding preferences. Here we describe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.7.1202
更新日期：1994-04-11 00:00:00
