Abstract：
:A highly polymorphic locus associated with the variable tandem repetition of a 35 bp consensus sequence was mapped to chromosome 10, band q26. Examination of leukocyte DNA from a cancer patient revealed the twenty-fold amplification of one allelic fragment of this locus, while the other allelic fragment demonstrated a normal copy number. In another patient, Southern blotting of leukocyte DNA detected the deletion of the 3'-flanking region from one tandem repeat allele. These results indicate that variable tandem repeats may mark highly unstable regions of DNA in the human genome which can be altered by changes more extensive than simple tandem repeat variation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Colb M,Yang-Feng T,Francke U,Mermer B,Parkinson DR,Krontiris TGdoi
10.1093/nar/14.20.7929subject
Has Abstractpub_date
1986-10-24 00:00:00pages
7929-37issue
20eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract：:The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.22.9087
更新日期：1989-11-25 00:00:00
abstract：:Bacterial Rho-independent terminators (RITs) are important genomic landmarks involved in gene regulation and terminating gene expression. In this investigation we present RNIE, a probabilistic approach for predicting RITs. The method is based upon covariance models which have been known for many years to be the most a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr168
更新日期：2011-08-01 00:00:00
abstract：:DEAD-box helicases catalyze the non-processive unwinding of double-stranded RNA (dsRNA) at the expense of adenosine triphosphate (ATP) hydrolysis. Nucleotide and RNA binding and unwinding are mediated by the RecA domains of the helicase core, but their cooperation in these processes remains poorly understood. We there...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku747
更新日期：2014-01-01 00:00:00
abstract：:The discoidin proteins of Dictyostelium discoideum are highly expressed during development. The Disc I gamma promoter allows the regulation of heterologous protein expression by experimental conditions. We report conditions under which the promoter activity is efficiently repressed during growth in the wildtype strain...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.6.1397
更新日期：1993-03-25 00:00:00
abstract：:Fluorescence anisotropy and fluorescence correlation spectroscopy measurements of rhodamine-labeled DNA oligonucleotide duplexes have been used to determine equilibrium binding constants for DNA binding of the prokaryotic transcription activator protein NtrC. Measurements were made with wild-type NtrC from Escherichia...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.6.1373
更新日期：1998-03-15 00:00:00
abstract：:Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ell...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.8.2089
更新日期：1976-08-01 00:00:00
abstract：:Simian virus 40 (SV40) nucleoprotein complexes were extracted from nuclei of infected monkey cells and fractionated on neutral sucrose density gradients. Complexes which contained replicating SV40 DNA (95S) separated well from those containing closed circular supercoiled viral DNA (75S). DNA polymerase activity was as...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.5.1715
更新日期：1978-05-01 00:00:00
abstract：:In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.8.1329
更新日期：1975-08-01 00:00:00
abstract：:Recent evidence indicates a link between Parkinson's Disease (PD) and the expression of a-synuclein (SNCA) isoforms with different 3' untranslated regions (3'UTRs). Yet, the post-transcriptional mechanisms regulating SNCA expression are unknown. Using a large-scale in vitro /in silico screening we identified RNA-bindi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1048
更新日期：2017-12-15 00:00:00
abstract：:MLL, the trithorax ortholog, is a well-characterized histone 3 lysine 4 methyltransferase that is crucial for proper regulation of the Hox genes during embryonic development. Chromosomal translocations, disrupting the Mll gene, lead to aggressive leukemia with poor prognosis. However, the functions of MLL in cellular ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku458
更新日期：2014-07-01 00:00:00
abstract：:Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine 5'-phosphorothioate (PhSpm3(2,2,7)G) as a key intermediate. This compound was activated by iodine in the presence of phosphoric acid an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.22.8979
更新日期：1989-11-25 00:00:00
abstract：:The cyanobacteirum Synechocystis sp. strain PCC 6803 possesses nine species of the sigma (sigma)-factor gene for RNA polymerase (RNAP). Here, we identify and characterize the novel-type promoter recognized by a group 3 sigma-factor, SigF. SigF autoregulates its own transcription and recognizes the promoter of pilA1 th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn453
更新日期：2008-09-01 00:00:00
abstract：:This study describes a novel functional property of the eukaryotic transcription factor NF-kappa B in altering the DNA structure. We observed that the binding of purified NF-kappa B to DNA was facilitated by spermine and cations which are known to promote DNA bending, and by a nick in one position at the 3'-end of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.22.6497
更新日期：1990-11-25 00:00:00
abstract：:A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like charac...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.2.689
更新日期：1979-02-01 00:00:00
abstract：:During translation initiation, eukaryotic initiation factor 2 (eIF2) delivers the Met-tRNA to the 40S ribosomal subunit to locate the initiation codon (AUGi) of mRNA during the scanning process. Stress-induced eIF2 phosphorylation leads to a general blockade of translation initiation and represents a key antiviral pat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw172
更新日期：2016-05-19 00:00:00
abstract：:Complementary DNA clones encoding the human kidney epidermal growth factor (EGF) precursor have been isolated and sequenced. They predict the sequence of a 1,207 amino acid protein which contains EGF flanked by polypeptide segments of 970 and 184 residues at its NH2- and COOH-termini, respectively. The structural orga...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.21.8427
更新日期：1986-11-11 00:00:00
abstract：:Expression of the wild-type p53 tumor suppressor gene has been found to play an important role in the regulation of cellular proliferation and differentiation. In addition, in many transformed cells and primary tumors, the gene has undergone allelic deletions and mutant forms of the p53 gene are expressed at elevated ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.2.345
更新日期：1993-01-25 00:00:00
abstract：:Gene trap mutagenesis of mouse embryonic stem cells generates random loss-of-function mutations, which can be identified by a sequence tag and can often report the endogenous expression of the mutated gene. The Centre for Modeling Human Disease is performing expression- and sequence-based screens of gene trap insertio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh106
更新日期：2004-01-01 00:00:00
abstract：:A simple, reproducible affinity chromatography method has been adapted for separation of high molecular weight supercoiled circular molecules from mammalian cells. Electron microscopic analysis of EB viral DNA obtained by this method, from the non-producer Burkitt's lymphoma line Raji, revealed monomer-sized viral mol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.24.10345
更新日期：1987-12-23 00:00:00
abstract：:Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infect...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.9.3119
更新日期：1979-07-11 00:00:00
abstract：:Phosphoprotein-binding domains (PPBDs) mediate many important cellular and molecular processes. Ten PPBDs have been known to exist in the human proteome, namely, 14-3-3, BRCT, C2, FHA, MH2, PBD, PTB, SH2, WD-40 and WW. PepCyber:P approximately PEP is a newly constructed database specialized in documenting human PPBD-c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm854
更新日期：2008-01-01 00:00:00
abstract：:The signal recognition particle database (SRPDB) is located at the University of Texas Health Science Center at Tyler and includes tabulations of SRP RNA, SRP protein and SRP receptor sequences. The sequences are annotated with links to the primary databases. They are ordered alphabetically or phylogenetically and are...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.1.169
更新日期：1999-01-01 00:00:00
abstract：:Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku105
更新日期：2014-04-01 00:00:00
abstract：:Homopolyribonucleotides and E. coli DNA wer spin labeled with an iodoacetamide-nitroxide compound. The extent of labeling is highly dependent upon the nature of the base and the secondary structure of the nucleic acid. This spin label-polymer linkage is unstable at high temperatures and in phosphate buffers. In order ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.4.847
更新日期：1976-04-01 00:00:00
abstract：:The satellite RNA of tobacco ringspot virus (STobRV RNA) replicates and becomes encapsidated in association with tobacco ringspot virus. Previous results show that the infected tissue produces multimeric STobRV RNAs of both polarities. RNA that is complementary to encapsidated STobRV RNA, designated as having the (-) ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.24.9729
更新日期：1986-12-22 00:00:00
abstract：:We have developed a new bioinformatics approach called ECMFinder (Evolutionary Conserved Motif Finder). This program searches for a given DNA motif within the entire genome of one species and uses the gene association information of a potential transcription factor-binding site (TFBS) to screen the homologous regions ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp077
更新日期：2009-04-01 00:00:00
abstract：:Plant specialized metabolites are chemically highly diverse, play key roles in host-microbe interactions, have important nutritional value in crops and are frequently applied as medicines. It has recently become clear that plant biosynthetic pathway-encoding genes are sometimes densely clustered in specific genomic lo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx305
更新日期：2017-07-03 00:00:00
abstract：:In the poly(A)+RNA isolated from the ciliate Paramecium primaurelia is found a discrete and abundant mRNA species of high molecular weight (corresponding to about 9,000 nucleotides). This mRNA species has size and abundance characteristics that identify it tentatively as the message coding for the variant cell-surface...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.21.7375
更新日期：1983-11-11 00:00:00
abstract：:Hammerhead ribozymes were transcribed from a dsDNA template containing four random nucleotides between stems II and III, which replace the naturally occurring GAA nucleotides. In vitro selection was used to select hammerhead ribozymes capable of in cis cleavage using denaturing polyacrylamide gels for the isolation of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.22.4401
更新日期：1996-11-15 00:00:00
abstract：:Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific nucleases such as Transcription Activator-Like Effectors Nucleases (TALENs) and Cas9-gRNA allows...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt555
更新日期：2013-10-01 00:00:00
