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Allosterism and signal transfer in DNA.

Abstract：

:We analysed the basic mechanisms of signal transmission in DNA and the origins of the allostery exhibited by systems such as the ternary complex BAMHI-DNA-GRDBD. We found that perturbation information generated by a primary protein binding event travels as a wave to distant regions of DNA following a hopping mechanism. However, such a structural perturbation is transient and does not lead to permanent changes in the DNA geometry and interaction properties at the secondary binding site. The BAMHI-DNA-GRDBD allosteric mechanism does not occur through any traditional models: direct (protein-protein), indirect (reorganization of the secondary site) readout or solvent-release. On the contrary, it is generated by a subtle and less common entropy-mediated mechanism, which might have an important role to explain other DNA-mediated cooperative effects.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Balaceanu A,Pérez A,Dans PD,Orozco M

doi

10.1093/nar/gky549

subject

Has Abstract

pub_date

2018-09-06 00:00:00

pages

7554-7565

issue

15

eissn

0305-1048

issn

1362-4962

pii

5037728

journal_volume

46

pub_type

杂志文章

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