Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR.

Abstract:

:A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Bonnet S,Prévot G,Bourgouin C

doi

10.1093/nar/26.4.1130

subject

Has Abstract

pub_date

1998-02-15 00:00:00

pages

1130-1

issue

4

eissn

0305-1048

issn

1362-4962

pii

gkb208

journal_volume

26

pub_type

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