Abstract:
:A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bonnet S,Prévot G,Bourgouin Cdoi
10.1093/nar/26.4.1130subject
Has Abstractpub_date
1998-02-15 00:00:00pages
1130-1issue
4eissn
0305-1048issn
1362-4962pii
gkb208journal_volume
26pub_type
杂志文章abstract::Transcription initiation complexes formed by bacterial RNA polymerases (RNAPs) exhibit dramatic species-specific differences in stability, leading to different strategies of transcription regulation. The molecular basis for this diversity is unclear. Promoter complexes formed by RNAP from Thermus aquaticus (Taq) are c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks973
更新日期:2012-12-01 00:00:00
abstract::Myotonic dystrophy type 1 (DM1) is an autosomal dominant multisystemic disorder caused by expansion of CTG triplet repeats in 3'-untranslated region of DMPK gene. The pathomechanism of DM1 is driven by accumulation of toxic transcripts containing expanded CUG repeats (CUG(exp)) in nuclear foci which sequester several ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv163
更新日期:2015-03-31 00:00:00
abstract::Mung bean nuclease hydrolyzes synthetic esters of 3'-nucleotides to nucleosides and phosphate esters; esters of 2'-nucleotides, and 2'--> 5' internucleotide linkages, are resistant. Esters of ribonucleotides are cleaved at 100-fold the rate for deoxyribonucleotides, the increased rate being due to presence of the 2'-h...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.5.699
更新日期:1974-05-01 00:00:00
abstract::RNA-binding proteins (RBPs) play important roles in the post-transcriptional control of RNAs. Identifying RBP binding sites and characterizing RBP binding preferences are key steps toward understanding the basic mechanisms of the post-transcriptional gene regulation. Though numerous computational methods have been dev...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1025
更新日期:2016-02-29 00:00:00
abstract::Differential expression analysis of RNA sequencing (RNA-seq) data typically relies on reconstructing transcripts or counting reads that overlap known gene structures. We previously introduced an intermediate statistical approach called differentially expressed region (DER) finder that seeks to identify contiguous regi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw852
更新日期:2017-01-25 00:00:00
abstract::RecBCD enzyme acts in the major pathway of homologous recombination of linear DNA in Escherichia coli. The enzyme unwinds DNA and is an ATP-dependent double-strand and single-strand exonuclease and a single-strand endonuclease; it acts at Chi recombination hotspots (5'-GCTGGTGG-3') to produce a recombinogenic single-s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.9.2125
更新日期:1998-05-01 00:00:00
abstract::Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are importan...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv152
更新日期:2015-04-20 00:00:00
abstract::To understand how potential for G-quadruplex formation might influence regulation of gene expression, we examined the 2 kb spanning the transcription start sites (TSS) of the 18 217 human RefSeq genes, distinguishing contributions of template and nontemplate strands. Regions both upstream and downstream of the TSS are...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1138
更新日期:2008-03-01 00:00:00
abstract::The HIRA histone chaperone complex deposits histone H3.3 into nucleosomes in a DNA replication- and sequence-independent manner. As herpesvirus genomes enter the nucleus as naked DNA, we asked whether the HIRA chaperone complex affects herpesvirus infection. After infection of primary cells with HSV or CMV, or transie...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx771
更新日期:2017-11-16 00:00:00
abstract::The oncogene BMI1 encodes a polycomb group transcription factor that is required for embryonic development and self-renewal of stem cells. Despite these important functions little is known about the regulation of BMI1 expression. A cDNA microarray based search for target genes of E2F-1 in neuroblastoma cells expressin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl119
更新日期:2006-03-31 00:00:00
abstract::The database of imprinted genes and parent-of-origin effects in animals (http://www.otago.ac.nz/IGC ) is a collation of genes and phenotypes for which parent-of-origin effects have been reported. The database currently includes over 220 entries, which describe over 40 imprinted genes in human, mouse and other animals....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.275
更新日期:2001-01-01 00:00:00
abstract:UNLABELLED:The PubMed® database of biomedical citations allows the retrieval of scientific articles studying the function of chemicals in biology and medicine. Mining millions of available citations to search reported associations between chemicals and topics of interest would require substantial human time. We have im...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku432
更新日期:2014-07-01 00:00:00
abstract::Rad18 is a ubiquitin E3 ligase that monoubiquitinates PCNA on stalled replications forks. This allows recruitment of damage-tolerant polymerases for damage bypass and DNA repair. In this activity, the Rad18 protein has to interact with Rad6, the E2 ubiquitin-conjugating enzyme, ubiquitin, PCNA and DNA. Here we analyze...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm615
更新日期:2007-01-01 00:00:00
abstract::Nucleoside 3'-phosphoramidite and chlorophosphite reagents have been found to react with the lactam function of guanine. This reaction caused unsatisfactory results when oligodeoxyribonucleotides containing a large number of guanine bases were prepared in an automated solid phase synthesizer. The guanine modification ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.18.6447
更新日期:1985-09-25 00:00:00
abstract::Here we report that the poor binding of methylphosphonate oligodeoxynucleosides (MP-ODNs) to their nucleic acid targets can be improved by additional inversion of the anomeric configuration (from beta to alpha) in the sugar moieties to give a new class of analogs, MP alpha-oligonucleosides. MP alpha-dT12and MP 5' alph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.20.4551
更新日期:1998-10-15 00:00:00
abstract::We report the nucleotide sequence of the Group IV RNA bacteriophage SP. The entire sequence is 4276 nucleotides long. Four cistrons have been identified by comparison with the related Group III phage Q beta. The maturation protein contains 449 amino acids, the coat protein contains 131 amino acids, the read-through pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.13.6205
更新日期:1988-07-11 00:00:00
abstract::In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.8.999
更新日期:1974-08-01 00:00:00
abstract::In conditions that mimic those of the living cell, where various biomolecules and other components are present, DNA strands can adopt many structures in addition to the canonical B-form duplex. Previous studies in the presence of cosolutes that induce molecular crowding showed that thermal stabilities of DNA structure...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1133
更新日期:2015-12-02 00:00:00
abstract::Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki486
更新日期:2005-07-01 00:00:00
abstract::Selenocysteine (Sec), the 21(st) amino acid in translation, uses its specific tRNA (tRNA(Sec)) to recognize the UGA codon. The Sec-specific elongation factor SelB brings the selenocysteinyl-tRNA(Sec) (Sec-tRNA(Sec)) to the ribosome, dependent on both an in-frame UGA and a Sec-insertion sequence (SECIS) in the mRNA. Th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv833
更新日期:2015-10-15 00:00:00
abstract::We have constructed a stable cell line, human embryonal kidney 293M+, containing a lacZ reporter gene controlled by an in vitro methylated hormone-responsive enhancer. Methylation of the enhancer-promoter abolishes lacZ expression controlled by ponasterone A (an analogue of ecdysone). Ponasterone A-induced expression ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.17.3603
更新日期:2001-09-01 00:00:00
abstract::A new E. coli strain has been constructed that contains the dinD1::LacZ+ fusion and is deficient in methylation-dependent restriction systems (McrA-, McrBC-, Mrr-). This strain has been used to clone restriction endonuclease genes directly into E. coli. When E. coli cells are not fully protected by the cognate methyla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.12.2399
更新日期:1994-06-25 00:00:00
abstract::We present the LGA (Local-Global Alignment) method, designed to facilitate the comparison of protein structures or fragments of protein structures in sequence dependent and sequence independent modes. The LGA structure alignment program is available as an online service at http://PredictionCenter.llnl.gov/local/lga. D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg571
更新日期:2003-07-01 00:00:00
abstract::The GWIPS-viz browser (http://gwips.ucc.ie/) is an on-line genome browser which is tailored for exploring ribosome profiling (Ribo-seq) data. Since its publication in 2014, GWIPS-viz provides Ribo-seq data for an additional 14 genomes bringing the current total to 23. The integration of new Ribo-seq data has been auto...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx790
更新日期:2018-01-04 00:00:00
abstract::Immunoglobulin (Ig) diversification by somatic hypermutation in germinal center B cells is instrumental for maturation of the humoral immune response, but also bears the risk of excessive or aberrant genetic changes. Thus, introduction of DNA damage by activation-induced cytidine deaminase as well as DNA repair by mul...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1378
更新日期:2014-04-01 00:00:00
abstract::Translation is a key regulatory step, linking transcriptome and proteome. Two major methods of translatome investigations are RNC-seq (sequencing of translating mRNA) and Ribo-seq (ribosome profiling). To facilitate the investigation of translation, we built a comprehensive database TranslatomeDB (http://www.translato...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1034
更新日期:2018-01-04 00:00:00
abstract::TFIIIC is a RNA polymerase (pol) III-specific DNA-binding factor that is required for transcription of tRNA and 5S rRNA genes. Active human TFIIIC consists of five subunits. However, an inactive form has also been isolated that lacks one of the five subunits, called TFIIIC110. A model was proposed in which pol III tra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl432
更新日期:2006-07-05 00:00:00
abstract::Enzymes involved in nucleic acid transactions often have a helicase-like ATPase coordinating and driving their functional activities, but our understanding of the mechanistic details of their coordination is limited. For example, DNA cleavage by the antiphage defense system Type ISP restriction-modification enzyme req...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa023
更新日期:2020-03-18 00:00:00
abstract::The X-ray crystal structure of the complex between the synthetic antitumour and antiviral DNA binding ligand SN 7167 and the DNA oligonucleotide d(CGCGAATTCGCG)2 has been determined to an R factor of 18.3% at 2.6 A resolution. The ligand is located within the minor groove and covers almost 6 bp with the 1-methylpyridi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.20.4072
更新日期:1997-10-15 00:00:00
abstract::Restriction fragment patterns of G+C-rich satellites of sheep and goat DNA were compared. The 1,712 g/cm3 satellites of both species appear homologous, consisting of repeats 760 base pairs long and showing coincidence of position of primary+ EcoRI, BamHI and most BspRI restriction target sites. The EcoRI and BamHI end...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.1.57
更新日期:1979-01-01 00:00:00