Abstract:
:Achiral pseudonucleosides bearing an anthraquinone moiety have been incorporated into deoxyoligonucleotides in internal, 3' and 5' positions. The ability of these modified deoxyoligonucleotides to hybridize to complementary RNA and DNA was investigated using Tm measurements. The anthraquinone was shown to enhance binding to a complementary RNA when linked to the 3' and 5' end. Pseudonucleoside substitutions on the 3' end of an oligonucleotide are also shown to confer serum stability to the oligonucleotide.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lin KY,Matteucci Mdoi
10.1093/nar/19.11.3111subject
Has Abstractpub_date
1991-06-11 00:00:00pages
3111-4issue
11eissn
0305-1048issn
1362-4962journal_volume
19pub_type
杂志文章abstract::The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucle...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.2.339
更新日期:1977-02-01 00:00:00
abstract::The IMGT/HLA database (http://www.ebi.ac.uk/imgt/hla) has provided a centralized repository for the sequences of the alleles named by the WHO Nomenclature Committee for Factors of the HLA System for the past four years. Since its initial release the database has grown and is the primary source of information for the s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg070
更新日期:2003-01-01 00:00:00
abstract::A specific segment of mitochondrial DNA from 18 people was examined by two methods of direct DNA sequencing. This segment includes a small noncoding region (V) shown before by restriction analysis to exhibit length polymorphism. All 11 of the human mtDNAs previously reported to have a deletion in this region proved to...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.2.529
更新日期:1987-01-26 00:00:00
abstract::We have investigated the substrate specificity of the Ogg1 protein of Saccharomyces cerevisiae (yOgg1 protein) for excision of modified DNA bases from oxidatively damaged DNA substrates using gas chromatography/isotope dilution mass spectrometry. Four DNA substrates prepared by treatment with H2O2/Fe(III)-EDTA/ascorbi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.5.1228
更新日期:1998-03-01 00:00:00
abstract::The molecular organization of the dunce gene of Drosophila melanogaster has proved to be particularly complex, with two divergently transcribed genes, Sgs-4 and Pig-1, nested within its 79 kb intron (1). Here we report the identification and the molecular characterization of a third gene nested within the transcriptio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.19.5837
更新日期:1990-10-11 00:00:00
abstract::Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we used a library scree...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr609
更新日期:2011-10-01 00:00:00
abstract:UNLABELLED:The PubMed® database of biomedical citations allows the retrieval of scientific articles studying the function of chemicals in biology and medicine. Mining millions of available citations to search reported associations between chemicals and topics of interest would require substantial human time. We have im...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku432
更新日期:2014-07-01 00:00:00
abstract::The titration viscometric investigation of the multi-mode interaction of netropsin (Nt) with (A.T) clusters of NaDNA12 and NH4DNA10 has been extended to different temperatures. The position of two boundaries on the r-scale (r= [Nt]bound/[DNA-P]) with increasing temperature steadily (rI/II) or more abruptly (rO/I) shif...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.10.2335
更新日期:1981-05-25 00:00:00
abstract::Core members of the MBD protein family (MeCP2, MBD1, MBD2 and MBD4) share a methyl-CpG-binding domain that has a specific affinity for methylated CpG sites in double-stranded DNA. By multimerizing the MDB domain of Mbd1, we engineered a poly-MBD protein that displays methyl-CpG-specific binding in vitro with a dissoci...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl527
更新日期:2006-08-07 00:00:00
abstract::Transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. The shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic DNA with in vitro packaging extracts. Mu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.10.3007
更新日期:1990-05-25 00:00:00
abstract::With the improved rapid sequencing techniques, the earlier sequence of U2 RNA of Novikoff hepatoma (Shibata et al, J. Biol. Chem. 250, 3909-3920, 1975) was reanalyzed and modified. The improved sequence of U2 RNA is 188 (or 189) nucleotides long and is in register with a characterized U2 RNA pseudogene (Denison et al,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.21.5645
更新日期:1981-11-11 00:00:00
abstract::A new method which predicts internal exon sequences in human DNA has been developed. The method is based on a splice site prediction algorithm that uses the linear discriminant function to combine information about significant triplet frequencies of various functional parts of splice site regions and preferences of ol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.24.5156
更新日期:1994-12-11 00:00:00
abstract::Human La protein is an essential factor in the biology of both coding and non-coding RNAs. In the nucleus, La binds primarily to 3' oligoU containing RNAs, while in the cytoplasm La interacts with an array of different mRNAs lacking a 3' UUU(OH) trailer. An example of the latter is the binding of La to the IRES domain...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr890
更新日期:2012-02-01 00:00:00
abstract::Epigenetics, and more specifically DNA methylation is a fast evolving research area. In almost every cancer type, each month new publications confirm the differentiated regulation of specific genes due to methylation and mention the discovery of novel methylation markers. Therefore, it would be extremely useful to hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm788
更新日期:2008-01-01 00:00:00
abstract::The precursor to the Euglena light harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) is a polyprotein containing multiple copies of LHCPII covalently joined by a decapeptide linker. cDNA and genomic clones encoding the 5' and 3' end of a 6.6 kb LHCPII mRNA were sequenced. A 3.1 kb genomic region en...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.25.5737
更新日期:1994-12-25 00:00:00
abstract::The (cytosine-5) DNA methyltransferase M.HhaI causes its target cytosine base to be flipped completely out of the DNA helix upon binding. We have investigated the effects of replacing the target cytosine by other, mismatched bases, including adenine, guanine, thymine and uracil. We find that M.HhaI binds more tightly ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.8.1388
更新日期:1995-04-25 00:00:00
abstract::The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initia...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.24.8677
更新日期:1983-12-20 00:00:00
abstract::Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisatio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.2.703
更新日期:1999-01-15 00:00:00
abstract::Two new endonuclease activities, endonuclease B and endonuclease C, obtained from yeast nuclear preparations have been separated and partially characterized. Endonuclease B has a primary requirement for Mn2+ which cannot be replaced by Mg2+ or Ca2+, and makes single-strand scissions in double-stranded DNA. Endonculeas...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.7.1023
更新日期:1975-07-01 00:00:00
abstract::The UCSC Genome Browser (https://genome.ucsc.edu) provides a web interface for exploring annotated genome assemblies. The assemblies and annotation tracks are updated on an ongoing basis-12 assemblies and more than 28 tracks were added in the past year. Two recent additions are a display of CRISPR/Cas9 guide sequences...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1020
更新日期:2018-01-04 00:00:00
abstract::As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.12.3233
更新日期:1992-06-25 00:00:00
abstract::A requirement for specific RNA folding is that the free-energy landscape discriminate against non-native folds. While tertiary interactions are critical for stabilizing the native fold, they are relatively non-specific, suggesting additional mechanisms contribute to tertiary folding specificity. In this study, we use ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1055
更新日期:2016-01-08 00:00:00
abstract::Rice alpha-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.23.7007
更新日期:1990-12-11 00:00:00
abstract::Splicing of mammalian introns requires that the intron possess at least 80 nucleotides. This length requirement presumably reflects the constraints of accommodating multiple snRNPs simultaneously in the same intron. In the free-living nematode, C. elegans, introns typically are 45 to 55 nucleotides in length. In this ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.1.143
更新日期:1990-01-11 00:00:00
abstract::TAP/hNXF1 is a key factor that mediates general cellular mRNA export from the nucleus, and its orthologs are structurally and functionally conserved from yeast to humans. Metazoans encode additional proteins that share homology and domain organization with TAP/hNXF1, suggesting their participation in mRNA metabolism; ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki706
更新日期:2005-07-13 00:00:00
abstract::PARP1 and PARP2 are implicated in the synthesis of poly(ADP-ribose) (PAR) after detection of DNA damage. The specificity of PARP1 and PARP2 interaction with long DNA fragments containing single- and/or double-strand breaks (SSBs and DSBs) have been studied using atomic force microscopy (AFM) imaging in combination wit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1476
更新日期:2016-04-07 00:00:00
abstract::The major surface antigen of procyclic and epimastigote forms of Trypanosoma congolense in the tsetse fly is GARP (glutamic acid/alanine-rich protein), which is thought to be the analogue of procyclin/PARP in Trypanosoma brucei. We have studied two T.congolense GARP loci (the 4.3 and 4.4 loci) whose transcription is a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.2.272
更新日期:1996-01-15 00:00:00
abstract::Aminoglycosides antibiotics negate dissociation and recycling of the bacterial ribosome's subunits by binding to Helix 69 (H69) of 23S rRNA. The differential binding of various aminoglycosides to the chemically synthesized terminal domains of the Escherichia coli and human H69 has been characterized using spectroscopy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1253
更新日期:2010-05-01 00:00:00
abstract::Gap closure is a challenging phase in microbial random shotgun genome sequencing projects, particularly since genome assemblies are often complicated by the presence of repeat elements, insertion sequences and other similar factors that contribute to sequence misassemblies. While it is well recognized that the conserv...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng107
更新日期:2003-09-01 00:00:00
abstract::Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa128
更新日期:2020-05-07 00:00:00