Abstract:
:We have determined the nucleotide sequence of the major species of E. coli tRNASer and of a minor species having the same GGA anticodon. These two tRNAs should recognize the UCC and UCU codons, the most widely used codons for serine in the highly expressed genes of E. coli. The two sequences differ in only one position of the D-loop. Neither tRNA has a modified adenosine in the position 3'-adjacent to the anticodon. This can be rationalized on the basis of a structural constraint in the anticodon stem and may be related to optimization of the codon-anticodon interaction. Comparison of all E.coli serine tRNAs (and that encoded by bacteriophage T4) reveals characteristic (possibly functional) features. Evolutionary analysis suggests an eubacterial origin of the T4 tRNASer gene and the existence of a recent common ancestor for the tRNASerGGA and tRNASerGUC genes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Grosjean H,Nicoghosian K,Haumont E,Söll D,Cedergren Rdoi
10.1093/nar/13.15.5697subject
Has Abstractpub_date
1985-08-12 00:00:00pages
5697-706issue
15eissn
0305-1048issn
1362-4962journal_volume
13pub_type
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