Abstract：
:DNA target recognizing domains of different multispecific DNA-cytosine-methyltransferases can be rearranged through engineering of the corresponding genes to generate enzymes with novel combinations of target recognition.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Trautner TA,Balganesh TS,Pawlek Bdoi
10.1093/nar/16.14.6649subject
Has Abstractpub_date
1988-07-25 00:00:00pages
6649-58issue
14Aeissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract：:Genome length complementary DNA (cDNA) transcripts were synthesized in vitro by using purified virions of a cloned isolate of mouse sarcoma virus (MSV Clone 124). The cDNA transcripts were converted to double-stranded form by utilizing DNase-digested calf thymus DNA primers and E. coli DNA polymerase I. Restriction en...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.5.1863
更新日期：1979-01-01 00:00:00
abstract：:Despite the increasing wealth of available data, the structure of cancer transcriptional space remains largely unknown. Analysis of this space would provide novel insights into the complexity of cancer, assess relative implications in complex biological processes and responses, evaluate the effectiveness of cancer mod...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr533
更新日期：2011-10-01 00:00:00
abstract：:Homo-oligomerization of proteins is abundant in nature, and is often intimately related with the physiological functions of proteins, such as in metabolism, signal transduction or immunity. Information on the homo-oligomer structure is therefore important to obtain a molecular-level understanding of protein functions ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx246
更新日期：2017-07-03 00:00:00
abstract：:The interactions of HIV-1 reverse transcriptase (HIV-1 RT) with a synthetic 53/19-mer DNA substrate was investigated. For this template-primer HIV-1 RT displayed a Km value of 20 nM. The 53/19-mer competitively inhibited DNA synthesis performed on poly (rC).oligo(dG) with Ki value of 260 nM. This corresponded well to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.17.3943
更新日期：1993-08-25 00:00:00
abstract：:Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw077
更新日期：2016-04-20 00:00:00
abstract：:TAP/hNXF1 is a key factor that mediates general cellular mRNA export from the nucleus, and its orthologs are structurally and functionally conserved from yeast to humans. Metazoans encode additional proteins that share homology and domain organization with TAP/hNXF1, suggesting their participation in mRNA metabolism; ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki706
更新日期：2005-07-13 00:00:00
abstract：:5' mediated cytoplasmic RNA decay is a conserved cellular process in eukaryotes. While the functions of the structured core domains in this pathway are well-studied, the role of abundant intrinsically disordered regions (IDRs) is lacking. Here we reconstitute the Dcp1:Dcp2 complex containing a portion of the disordere...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky233
更新日期：2018-07-06 00:00:00
abstract：:The interplay between aromatic stacking and hydrogen bonding in nucleobases has been investigated via high-level quantum chemical calculations. The experimentally observed stacking arrangement between consecutive bases in DNA and RNA/DNA double helices is shown to enhance their hydrogen bonding ability as opposed to g...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki317
更新日期：2005-03-23 00:00:00
abstract：:LINE-1 (L1) is a highly successful autonomous non-LTR retrotransposon and a major force shaping mammalian genomes. Although there are about 600 000 L1 copies covering 23% of the rat genome, full-length rat L1s (L1Rn) with intact open reading frames (ORFs) representing functional master copies for retrotransposition ha...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1045
更新日期：2008-02-01 00:00:00
abstract：:Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1146
更新日期：2018-01-25 00:00:00
abstract：:The EcoCyc database (http://EcoCyc.org/) is a comprehensive source of information on the biology of the prototypical model organism Escherichia coli K12. The mission for EcoCyc is to contain both computable descriptions of, and detailed comments describing, all genes, proteins, pathways and molecular interactions in E...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki108
更新日期：2005-01-01 00:00:00
abstract：:MicroRNA 26a (miR-26a) reduces cell viability in several cancers, indicating that miR-26a could be used as a therapeutic option in patients. We demonstrate that miR-26a not only inhibits G1-S cell cycle transition and promotes apoptosis, as previously described, but also regulates multiple cell cycle checkpoints. We s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx022
更新日期：2017-05-05 00:00:00
abstract：:One of the master regulators of placental cell fusion in mammals leading to multi-nucleated syncytiotrophoblasts is the transcription factor GCMa. Recently, we proved that the cAMP-driven protein kinase A signaling pathway is fundamental for up-regulation of GCMa transcript levels and protein stability. Here, we show ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn306
更新日期：2008-06-01 00:00:00
abstract：:The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using (32)P-labeled material from T4-infected cultures of Escherichiacoli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAApsiCUGAUGAUGUGAGTpsiCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the class...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.10.1287
更新日期：1974-10-01 00:00:00
abstract：:This study describes a novel functional property of the eukaryotic transcription factor NF-kappa B in altering the DNA structure. We observed that the binding of purified NF-kappa B to DNA was facilitated by spermine and cations which are known to promote DNA bending, and by a nick in one position at the 3'-end of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.22.6497
更新日期：1990-11-25 00:00:00
abstract：:The evolution of regulatory networks in Bacteria has largely been explained at macroevolutionary scales through lateral gene transfer and gene duplication. Transcription factors (TF) have been found to be less conserved across species than their target genes (TG). This would be expected if TFs accumulate mutations fas...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa162
更新日期：2020-05-07 00:00:00
abstract：:Hfq, a bacterial RNA-binding protein, was recently shown to contain the Sm1 motif, a characteristic of Sm and LSm proteins that function in RNA processing events in archaea and eukaryotes. In this report, comparative structural modeling was used to predict a three-dimensional structure of the Hfq core sequence. The pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf508
更新日期：2002-09-01 00:00:00
abstract：:Protein-based methods of siRNA delivery are capable of uniquely specific targeting, but are limited by technical challenges such as low potency or poor biophysical properties. Here, we engineered a series of ultra-high affinity siRNA binders based on the viral protein p19 and developed them into siRNA carriers targete...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx546
更新日期：2017-07-27 00:00:00
abstract：:RNA sequencing has become an important method to perform hypothesis-free characterization of global gene expression. One of the limitations of RNA sequencing is that most sequence reads represent highly expressed transcripts, whereas low level transcripts are challenging to detect. To combine the benefits of tradition...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks816
更新日期：2013-01-07 00:00:00
abstract：:Tandem repeats in DNA have been under intensive study for many years, first, as a consequence of their usefulness as genomic markers and DNA fingerprints and more recently as their role in human disease and regulatory processes has become apparent. The Tandem Repeats Database (TRDB) is a public repository of informati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1013
更新日期：2007-01-01 00:00:00
abstract：:A growing body of evidence suggests that genes transcribed by RNA polymerase III exhibit multiple functions within a chromosome. While the predominant function of these genes is the synthesis of RNA molecules, certain RNA polymerase III genes also function as genomic landmarks. Transfer RNA genes are known to exhibit ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh858
更新日期：2004-09-30 00:00:00
abstract：:Recombinase proteins assembled into helical filaments on DNA are believed to be the catalytic core of homologous recombination. The assembly, disassembly and dynamic rearrangements of this structure must drive the DNA strand exchange reactions of homologous recombination. The sensitivity of eukaryotic recombinase acti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki640
更新日期：2005-06-08 00:00:00
abstract：:The chicken erythroid proteins binding to the histone H5 5' promoter and 3' erythroid-specific enhancer regions were identified. In DNase I footprinting and gel mobility shift experiments with immature adult erythrocyte nuclear extracts, we have demonstrated the binding of proteins to the GC-box, a high affinity Sp1 b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.23.6385
更新日期：1992-12-11 00:00:00
abstract：:Nucleic acid double helices are stabilized by hydrogen bonding and stacking forces (a combination of hydrophobic, dispersive and electrostatic forces) of the base pairs in the helix. One would predict the hydrogen bonding contributions to increase and the stacking contributions to decrease as the water activity in the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm210
更新日期：2007-01-01 00:00:00
abstract：:A database (http://perso.curie.fr/tsoussi ) is described, in which over 1000 mutations in the human APC gene of tumors (colon cancer predominantly) are compiled from the literature. It includes both molecular information about the mutations and clinical data about the patients. Software has been designed to analyse al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.269
更新日期：1998-01-01 00:00:00
abstract：:The reported binding preference of human hnRNP protein A1 for the 3'-splice site of some introns (Swanson and Dreyfuss (1988) EMBO J. 7, 3519-3529; Mayrand and Pederson (1990) Nucleic Acids Res. 18, 3307-3318) was tested by assaying in vitro the binding of purified recombinant A1 protein (expressed in bacteria) to syn...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.22.6595
更新日期：1990-11-25 00:00:00
abstract：:Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq801
更新日期：2011-01-01 00:00:00
abstract：:We have carried out a survey of potential quadruplex structure sequences (PQSS), which occur in the immediate upstream region (500 bp) of human genes. By examining the number and distribution of these we have established that there is a clear link between them and the occurrence of the SP1-binding element 'GGGCGG', su...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn078
更新日期：2008-05-01 00:00:00
abstract：:Polyadenylation is a co-transcriptional process that modifies mRNA 3'-ends in eukaryotes. In yeast, CF IA and CPF constitute the core 3'-end maturation complex. CF IA comprises Rna14p, Rna15p, Pcf11p and Clp1p. CF IA interacts with the C-terminal domain of RNA Pol II largest subunit via Pcf11p which links pre-mRNA 3'-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr800
更新日期：2012-02-01 00:00:00
abstract：:The Gene Context Tool (GeConT) allows users to visualize the genomic context of a gene or a group of genes and their orthologous relationships within fully sequenced bacterial genomes. The new version of the server incorporates information from the COG, Pfam and KEGG databases, allowing users to have an integrated gra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn330
更新日期：2008-07-01 00:00:00
