Abstract:
:An efficient procedure for the synthesis of unnatural alpha-anomeric oligodeoxyribonucleotides is described. This solid-phase procedure is based on the use of alpha-nucleoside phosphoramidites and alpha-nucleoside derivatized solid supports corresponding to the four natural bases and allow rapid synthesis of oligonucleotides up to 20 alpha-deoxynucleotide units in length. After HPLC purification, a 15-mer: alpha-d(CCTCTCGTTCTTTAC) and a 20-mer: alpha-d(ATACTTGAGGAAGAGGTGTT) were obtained respectively in 27 and 29% overall yields. Their purity, nucleoside composition and primary structure were ascertained by HPLC and Maxam-Gilbert sequence analyses.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Morvan F,Rayner B,Leonetti JP,Imbach JLdoi
10.1093/nar/16.3.833subject
Has Abstractpub_date
1988-02-11 00:00:00pages
833-47issue
3eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::BZ junctions, which connect B-DNA to Z-DNA, are necessary for local transformation of B-DNA to Z-DNA in the genome. However, the limited information on the junction-forming sequences and junction structures has led to a lack of understanding of the structural diversity and sequence preferences of BZ junctions. We dete...
journal_title:Nucleic acids research
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doi:10.1093/nar/gky784
更新日期:2018-11-02 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr208
更新日期:2011-08-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and micro-sequen...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.9.4011
更新日期:1984-05-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-07-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
abstract::In many eukaryotic mRNAs one or more short 'upstream' open reading frames, uORFs, precede the initiator of the main coding sequence. Upstream ORFs are functionally diverse as illustrated by their variety of features in polyamine pathway biosynthetic mRNAs. Their propensity to act as sensors for regulatory circuits and...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2010-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2012-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.3.789
更新日期:1978-03-01 00:00:00
abstract::Bulk replicative DNA synthesis in eukaryotes is highly accurate and efficient, primarily because of two DNA polymerases (Pols): Pols δ and ε. The high fidelity of these enzymes is due to their intrinsic base selectivity and proofreading exonuclease activity which, when coupled with post-replication mismatch repair, he...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.12.4479
更新日期:1978-12-01 00:00:00
abstract::We present FIGfams, a new collection of over 100,000 protein families that are the product of manual curation and close strain comparison. Using the Subsystem approach the manual curation is carried out, ensuring a previously unattained degree of throughput and consistency. FIGfams are based on over 950,000 manually a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp698
更新日期:2009-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1089
更新日期:2014-12-01 00:00:00
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更新日期:1985-12-09 00:00:00
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journal_title:Nucleic acids research
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更新日期:2019-09-26 00:00:00
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journal_title:Nucleic acids research
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更新日期:2003-06-15 00:00:00
abstract::A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size an...
journal_title:Nucleic acids research
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doi:10.1093/nar/10.17.5223
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1994-12-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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doi:10.1093/nar/gky1029
更新日期:2019-01-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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