The Wilms' tumor suppressor gene (wt1) product represses different functional classes of transcriptional activation domains.

abstract：:During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluorescence signal emitted ...

journal_title：Nucleic acids research

authors： Haase N,Holtkamp W,Lipowsky R,Rodnina M,Rudorf S

更新日期：2018-12-14 00:00:00

abstract：:Genomes mutate and evolve in ways simple (substitution or deletion of bases) and complex (e.g. chromosome shattering). We do not fully understand what types of complex mutation occur, and we cannot routinely characterize arbitrarily-complex mutations in a high-throughput, genome-wide manner. Long-read DNA sequencing m...

journal_title：Nucleic acids research

authors： Frith MC,Khan S

更新日期：2018-02-28 00:00:00

abstract：:Genetic screens in Saccharomyces cerevisiae have identified the roles of ribosome components, tRNAs and translation factors in translational fidelity. These screens rely on the suppression of altered start codons, nonsense codons or frameshift mutations in genes involved in amino acid or nucleotide metabolism. Many of...

journal_title：Nucleic acids research

authors： Magazinnik T,Anand M,Sattlegger E,Hinnebusch AG,Kinzy TG

更新日期：2005-08-12 00:00:00

abstract：:OrthoDB is a comprehensive catalog of orthologs, genes inherited by extant species from a single gene in their last common ancestor. In 2016 OrthoDB reached its 9th release, growing to over 22 million genes from over 5000 species, now adding plants, archaea and viruses. In this update we focused on usability of this f...

journal_title：Nucleic acids research

authors： Zdobnov EM,Tegenfeldt F,Kuznetsov D,Waterhouse RM,Simão FA,Ioannidis P,Seppey M,Loetscher A,Kriventseva EV

更新日期：2017-01-04 00:00:00

abstract：:Human papillomavirus 16 (HPV-16), which is involved in genital carcinogenesis, contains an enhancer of transcription that is activated by cellular factors rather than by the viral E2 proteins. The activity resides on a 232 bp segment with 5 binding sites for nuclear factor 1 (NF1), 2 for AP1, and 1 for steroid recepto...

journal_title：Nucleic acids research

authors： Chong T,Chan WK,Bernard HU

更新日期：1990-02-11 00:00:00

abstract：:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisel...

journal_title：Nucleic acids research

authors： Jaye M,de la Salle H,Schamber F,Balland A,Kohli V,Findeli A,Tolstoshev P,Lecocq JP

更新日期：1983-04-25 00:00:00

abstract：:In the haploid genome of Xenopus laevis there are two genes coding for the r-protein L14. It is not known if they are located on the same chromosome. cDNA clones deriving from the transcripts of the two genes have been isolated from an oocyte messenger cDNA bank showing that they are both expressed. We have studied th...

journal_title：Nucleic acids research

authors： Beccari E,Mazzetti P,Mileo A,Bozzoni I,Pierandrei-Amaldi P,Amaldi F

更新日期：1986-10-10 00:00:00

abstract：:Transcriptional regulatory networks specify regulatory proteins controlling the context-specific expression levels of genes. Inference of genome-wide regulatory networks is central to understanding gene regulation, but remains an open challenge. Expression-based network inference is among the most popular methods to i...

journal_title：Nucleic acids research

authors： Siahpirani AF,Roy S

更新日期：2017-02-28 00:00:00

abstract：:Struct2Net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. Prediction of protein-protein interactions (PPIs) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of t...

journal_title：Nucleic acids research

authors： Singh R,Park D,Xu J,Hosur R,Berger B

更新日期：2010-07-01 00:00:00

abstract：:Gap closure is a challenging phase in microbial random shotgun genome sequencing projects, particularly since genome assemblies are often complicated by the presence of repeat elements, insertion sequences and other similar factors that contribute to sequence misassemblies. While it is well recognized that the conserv...

journal_title：Nucleic acids research

authors： Herron-Olson L,Freeman J,Zhang Q,Retzel EF,Kapur V

更新日期：2003-09-01 00:00:00

abstract：:DNA polymerase beta (polbeta), a member of the X family of DNA polymerases, is the major polymerase in the base excision repair pathway. Using in vitro selection, we obtained RNA aptamers for polbeta from a variable pool of 8 x 10(12) individual RNA sequences containing 30 random nucleotides. A total of 60 individual ...

journal_title：Nucleic acids research

authors： Gening LV,Klincheva SA,Reshetnjak A,Grollman AP,Miller H

更新日期：2006-05-17 00:00:00

abstract：:We show that in mouse myoblasts the MyoD1 promoter is highly stimulated by MyoD1 expression, suggesting that it is controlled by a positive feedback loop. Using deletion and mutation analyses, we identified the targets for MyoD1 promoter autoregulation as the two proximal E-boxes located close to the MyoD1 core promot...

journal_title：Nucleic acids research

authors： Zingg JM,Pedraza-Alva G,Jost JP

更新日期：1994-06-25 00:00:00

abstract：:Recently, we described a method for multiplex genome editing by natural transformation (MuGENT). Mutant constructs for MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro DNA splicing. In Vibrio cholerae, we uncover that this requirement is due to cytopl...

journal_title：Nucleic acids research

authors： Dalia TN,Yoon SH,Galli E,Barre FX,Waters CM,Dalia AB

更新日期：2017-07-07 00:00:00

abstract：:TGGCA-binding proteins are nuclear proteins with high affinity for double-stranded DNA homologous to the prototype recognition sequence 5'YTGGCANNNTGCCAR 3'. Their ubiquitous tissue distribution in higher vertebrates characterizes them as a class of highly conserved proteins which may exert a basic function. To obtain...

journal_title：Nucleic acids research

authors： Nowock J,Borgmeyer U,Püschel AW,Rupp RA,Sippel AE

更新日期：1985-03-25 00:00:00

abstract：:Differential melting profiles and denaturation maps are calculated for fdDNA whose sequence of nucleotides has been determined recently. The melting profiles for the total DNA and a number of its restriction fragments are compared with experimental data taken from literature. The comparison enables one to correlate a ...

journal_title：Nucleic acids research

authors： Vologodskii AV,Frank-Kamenetskii MD

更新日期：1978-07-01 00:00:00

abstract：:We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome c...

journal_title：Nucleic acids research

authors： Breindl M,Kalthoff H,Jaenisch R

更新日期：1983-06-25 00:00:00

abstract：:To bridge the gap between the rising information needs of biological and medical researchers and the rapidly growing number of online bioinformatics resources, we have created the Online Bioinformatics Resources Collection (OBRC) at the Health Sciences Library System (HSLS) at the University of Pittsburgh. The OBRC, c...

journal_title：Nucleic acids research

authors： Chen YB,Chattopadhyay A,Bergen P,Gadd C,Tannery N

更新日期：2007-01-01 00:00:00

abstract：:The analysis of chromatin fine structure and transcription factor occupancy of differentially expressed genes by in vivo footprinting and ligation-mediated-PCR (LMPCR) is a powerful tool to understand the impact of chromatin on gene expression. However, as with all PCR-based techniques, the accuracy of the experiments...

journal_title：Nucleic acids research

authors： Ingram R,Gao C,Lebon J,Liu Q,Mayoral RJ,Sommer SS,Hoogenkamp M,Riggs AD,Bonifer C

更新日期：2008-02-01 00:00:00

abstract：:In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneou...

journal_title：Nucleic acids research

authors： Gambaryan AS,Venkstern TV,Bayev AA

更新日期：1976-08-01 00:00:00

abstract：:The stability of chromosome ends, the telomeres, is dependent on the ribonucleoprotein telomerase. In vitro, telomerase requires at least one RNA molecule and a reverse transcriptase-like protein. However, for telomere homeostasis in vivo, additional proteins are required. Telomerase RNAs of different species vary in ...

journal_title：Nucleic acids research

authors： Laterreur N,Eschbach SH,Lafontaine DA,Wellinger RJ

更新日期：2013-09-01 00:00:00

abstract：:The side chains of the 20 types of amino acids, owing to a large extent to their different physical properties, have characteristic distributions in interior/surface regions of individual proteins and in interface/non-interface portions of protein surfaces that bind proteins or nucleic acids. These distributions have ...

journal_title：Nucleic acids research

authors： Tjong H,Qin S,Zhou HX

更新日期：2007-07-01 00:00:00

abstract：:In HIV-1 infection, Tat acts at least in part to control transcriptional elongation by overcoming premature transcriptional termination. In some other genes this process is governed by DNA elements called attenuators in concert with cellular transcription factors. To understand the action of Tat more fully and explore...

journal_title：Nucleic acids research

authors： Greenberg ME,Mathews MB

更新日期：1997-12-15 00:00:00

abstract：:We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit beta-globin gene. In most somatic tissues, including erythroid and non-erythroid tissues, about 50% of the DNA is resistant to cleavage at this site by HapII, though 100% cleavage is found with the isoschiz...

journal_title：Nucleic acids research

authors： Waalwijk C,Flavell RA

更新日期：1978-12-01 00:00:00

abstract：:CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems allow bacteria to adapt to infection by acquiring 'spacer' sequences from invader DNA into genomic CRISPR loci. Cas proteins use RNAs derived from these loci to target cognate sequences for destruction through CRISPR inter...

journal_title：Nucleic acids research

authors： Xue C,Seetharam AS,Musharova O,Severinov K,Brouns SJ,Severin AJ,Sashital DG

更新日期：2015-12-15 00:00:00

abstract：:PAHdb (http://www.mcgill.ca/pahdb ) is a curated relational database (Fig. 1) of nucleotide variation in the human PAH cDNA (GenBank U49897). Among 328 different mutations by state (Fig. 2) the majority are rare mutations causing hyperphenylalaninemia (HPA) (OMIM 261600), the remainder are polymorphic variants without...

journal_title：Nucleic acids research

authors： Nowacki PM,Byck S,Prevost L,Scriver CR

更新日期：1998-01-01 00:00:00

abstract：:Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses T4 RNA ligase to attach a 3'-biotinylated donor molecule to target RNA. Microarray analyses indicate that directly labeled RNA is...

journal_title：Nucleic acids research

authors： Cole K,Truong V,Barone D,McGall G

更新日期：2004-06-17 00:00:00

abstract：:Yeast tRNA Val 1 alkylation with 2', 3'-O-4-(N-2-chloroethyl-N-methylamino) benzylidene d(pC-G)-A proceeds at 20 degrees - 30 degrees C in the complementary complexes which are formed by d(pC-G)-A greater than RC1 binding to 3 sequences of tRNA Val 1 : psi-C-G58 in the T loop, C-G40 at the 3'-side of the anticodon loo...

journal_title：Nucleic acids research

authors： Grineva NI,Karpova GG,Kuznetsova LM,Venkstern TV,Bayev AA

更新日期：1977-01-01 00:00:00

abstract：:Protein-RNA interactions in small nuclear ribonucleoproteins (UsnRNPs) from HeLa cells were investigated by irradiation of purified nucleoplasmic snRNPs U1 to U6 with UV light at 254 nm. The cross-linked proteins were analyzed on one- and two-dimensional gel electrophoresis systems, and the existence of a stable cross...

journal_title：Nucleic acids research

authors： Woppmann A,Rinke J,Lührmann R

更新日期：1988-12-09 00:00:00

abstract：:The c-myc proto-oncogene encodes a highly unstable mRNA. Stabilized, truncated myc transcripts have been found in several human and murine tumors of hematopoietic origin. Recently, two tumors expressing 3' truncated c-myc mRNAs that were five times more stable than normal myc transcripts, were described. We have tried...

journal_title：Nucleic acids research

authors： Laird-Offringa IA,Elfferich P,van der Eb AJ

更新日期：1991-05-11 00:00:00

abstract：:The universal L-shaped tertiary structure of tRNAs is maintained with the help of nucleotide modifications within the D- and T-loops, and these modifications are most extensive within hyperthermophilic species. The obligate-commensal Nanoarchaeum equitans and its phylogenetically-distinct host Ignicoccus hospitalis gr...

journal_title：Nucleic acids research

authors： Rose S,Auxilien S,Havelund JF,Kirpekar F,Huber H,Grosjean H,Douthwaite S

更新日期：2020-07-09 00:00:00