The TGGCA protein binds to the MMTV-LTR, the adenovirus origin of replication, and the BK virus enhancer.

abstract：:Phosphorylation of the cellular single-stranded DNA-binding protein, replication protein A (RPA), occurs during normal mitotic cell cycle progression and also in response to genotoxic stress. In budding yeast, these reactions require the ATM homolog Mec1, a central regulator of the DNA replication and DNA damage check...

journal_title：Nucleic acids research

authors： Brush GS,Clifford DM,Marinco SM,Bartrand AJ

更新日期：2001-12-01 00:00:00

abstract：:Correlation of motif occurrences with gene expression intensity is an effective strategy for elucidating transcriptional cis-regulatory logic. Here we demonstrate that this approach can also identify cis-regulatory elements for alternative pre-mRNA splicing. Using data from a human exon microarray, we identified 56 ca...

journal_title：Nucleic acids research

authors： Das D,Clark TA,Schweitzer A,Yamamoto M,Marr H,Arribere J,Minovitsky S,Poliakov A,Dubchak I,Blume JE,Conboy JG

更新日期：2007-01-01 00:00:00

abstract：:Under-modified E. coli tRNATyr that contains 7-(aminomethyl)-7-deazaguanosine in place of Q nucleoside can be chemically modified by dansyl chloride under neutral conditions. Fluorescent labelling specifically occurred only in the 7-(aminomethyl)-7-deazaguanine moiety. The modified tRNATyr was found to be active both ...

journal_title：Nucleic acids research

authors： Kasai H,Shindo-Okada N,Noguchi S,Nishimura S

更新日期：1979-09-11 00:00:00

abstract：:Silencing gene expression by siRNAs is rapidly becoming a powerful tool for the genetic analysis of mammalian cells. However, the rapid degradation of siRNA and the limited duration of its action call for an efficient delivery technology. Accordingly, we describe here that Atelocollagen complexed with siRNA is resista...

journal_title：Nucleic acids research

authors： Minakuchi Y,Takeshita F,Kosaka N,Sasaki H,Yamamoto Y,Kouno M,Honma K,Nagahara S,Hanai K,Sano A,Kato T,Terada M,Ochiya T

更新日期：2004-07-22 00:00:00

abstract：:We present a study of how substituent groups of naturally occurring and modified nucleotide bases affect the degree of hydration of right-handed B-DNA and left-handed Z-DNA. A comparison of poly(dG-dC) and poly(dG-dm5C) titrations with the lipotropic salts of the Hofmeister series infers that the methyl stabilization ...

journal_title：Nucleic acids research

authors： Kagawa TF,Howell ML,Tseng K,Ho PS

更新日期：1993-12-25 00:00:00

abstract：:DNA synthesized by in vitro transcription on rat interferon (IFN) mRNA has been cloned and amplified as recombinant DNA. The nucleotide sequence of these rat IFN cDNA clones revealed i. the partial presence of the coding region of the gene and ii all cDNA clones were derived from the same subtype of rat IFN-alpha mRNA...

journal_title：Nucleic acids research

authors： Dijkema R,Pouwels P,de Reus A,Schellekens H

更新日期：1984-01-25 00:00:00

abstract：:VapD-like virulence-associated proteins have been found in many organisms, but little is known about this protein family including the 3D structure of these proteins. Recently, a relationship between the Cas2 family of ribonucleases associated with the CRISPR system of microbial immunity and VapD was suggested. Here, ...

journal_title：Nucleic acids research

authors： Kwon AR,Kim JH,Park SJ,Lee KY,Min YH,Im H,Lee I,Lee KY,Lee BJ

更新日期：2012-05-01 00:00:00

abstract：:MicroRNAs (miRNAs) are short, non-protein coding RNAs that direct the widespread phenomenon of post-transcriptional regulation of metazoan genes. The mature approximately 22-nt long RNA molecules are processed from genome-encoded stem-loop structured precursor genes. Hundreds of such genes have been experimentally val...

journal_title：Nucleic acids research

authors： Gerlach D,Kriventseva EV,Rahman N,Vejnar CE,Zdobnov EM

更新日期：2009-01-01 00:00:00

abstract：:Basic region-leucine zipper (B-ZIP) proteins are a class of dimeric sequence-specific DNA-binding proteins unique to eukaryotes. We have identified 67 B-ZIP proteins in the Arabidopsis thaliana genome. No A.thaliana B-ZIP domains are homologous with any Homo sapiens B-ZIP domains. Here, we predict the dimerization spe...

journal_title：Nucleic acids research

authors： Deppmann CD,Acharya A,Rishi V,Wobbes B,Smeekens S,Taparowsky EJ,Vinson C

更新日期：2004-06-29 00:00:00

abstract：:A number of novel phosphate triester derivatives of the anti-cancer nucleoside analogue araC have been prepared by a rapid 2-step procedure, not necessitating prior sugar protection. Spectroscopic and lipophilicity data have been collected on these compounds. An in vitro assay indicated inhibition of thymidine incorpo...

journal_title：Nucleic acids research

authors： Jones BC,McGuigan C,Riley PA

更新日期：1989-09-25 00:00:00

abstract：:Structured RNA molecules form complex 3D architectures stabilized by multiple interactions involving the nucleotide base, sugar and phosphate moieties. A significant percentage of the bases in structured RNA molecules in the Protein Data Bank (PDB) hydrogen-bond with phosphates of other nucleotides. By extracting and ...

journal_title：Nucleic acids research

authors： Zirbel CL,Sponer JE,Sponer J,Stombaugh J,Leontis NB

更新日期：2009-08-01 00:00:00

abstract：:The endoribonuclease RNase E is a key enzyme in RNA metabolism for many bacterial species. In Escherichia coli, RNase E contributes to the majority of RNA turnover and processing events, and the enzyme has been extensively characterized as the central component of the RNA degradosome assembly. A similar RNA degradosom...

journal_title：Nucleic acids research

authors： Voss JE,Luisi BF,Hardwick SW

更新日期：2014-12-01 00:00:00

abstract：:We have determined the sequence of 80 nucleotides adjacent to 3' poly(A) of both (middle and bottom component) cowpea mosaic virus RNAs, using dideoxynucleotide termination of reverse transcription. Sequence conservation is indicated, there being about 80% homology between the first 65 bases of each RNA. Although both...

journal_title：Nucleic acids research

authors： Davies JW,Stanley J,Van Kammen A

更新日期：1979-09-25 00:00:00

abstract：:The nucleotide sequence of the Anacystis nidulans 23S rRNA gene, including the 5'- and 3'-flanking regions has been determined. The gene is 2876 nucleotides long and shows higher primary sequence homology to the 23S rRNAs of plastids (84.5%) than to that of E. coli (79%). The predicted rRNA transcript also shares many...

journal_title：Nucleic acids research

authors： Douglas SE,Doolittle WF

更新日期：1984-04-11 00:00:00

abstract：:Recruitment of RAD18 to stalled replication forks facilitates monoubiquitination of PCNA during S-phase, promoting translesion synthesis at sites of UV irradiation-induced DNA damage. In this study, we show that RAD18 is also recruited to ionizing radiation (IR)-induced sites of DNA double-strand breaks (DSBs) forming...

journal_title：Nucleic acids research

authors： Watanabe K,Iwabuchi K,Sun J,Tsuji Y,Tani T,Tokunaga K,Date T,Hashimoto M,Yamaizumi M,Tateishi S

更新日期：2009-04-01 00:00:00

abstract：:The four Hu [embryonic lethal abnormal vision-like (ELAVL)] protein family members regulate alternative splicing by binding to U-rich sequences surrounding target exons and affecting the interaction of the splicing machinery and/or local chromatin modifications. Each of the Hu proteins contains a divergent N-terminus,...

journal_title：Nucleic acids research

authors： Hinman MN,Zhou HL,Sharma A,Lou H

更新日期：2013-05-01 00:00:00

abstract：:The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2-10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of...

journal_title：Nucleic acids research

authors： Hardwick JS,Haugland MM,El-Sagheer AH,Ptchelkine D,Beierlein FR,Lane AN,Brown T,Lovett JE,Anderson EA

更新日期：2020-04-06 00:00:00

abstract：:TATA-binding protein (TBP) is central to the regulation of eukaryotic transcription initiation. Recruitment of TBP to target genes can be positively regulated by one of two basal transcription factor complexes: SAGA or TFIID. Negative regulation of TBP promoter association can be performed by Mot1p or the NC2 complex....

journal_title：Nucleic acids research

authors： Spedale G,Meddens CA,Koster MJ,Ko CW,van Hooff SR,Holstege FC,Timmers HT,Pijnappel WW

更新日期：2012-02-01 00:00:00

abstract：:The pattern of preferential DNA repair of UV-induced pyrimidine dimers was studied in repair-deficient Chinese hamster ovary (CHO) cells transfected with the human excision repair gene, ERCC-1. Repair efficiency was measured in the active dihydrofolate reductase (DHFR) gene and in its flanking, non-transcribed sequenc...

journal_title：Nucleic acids research

authors： Bohr VA,Chu EH,van Duin M,Hanawalt PC,Okumoto DS

更新日期：1988-08-11 00:00:00

abstract：:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisel...

journal_title：Nucleic acids research

authors： Jaye M,de la Salle H,Schamber F,Balland A,Kohli V,Findeli A,Tolstoshev P,Lecocq JP

更新日期：1983-04-25 00:00:00

abstract：:A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA produced by E. coli ...

journal_title：Nucleic acids research

authors： Duggleby CJ,Jones SA

更新日期：1983-05-25 00:00:00

abstract：:Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light microscopic particle observation is usually limited to two-dimensional projections of short trajectory segments, we report here imag...

journal_title：Nucleic acids research

authors： Spille JH,Kaminski TP,Scherer K,Rinne JS,Heckel A,Kubitscheck U

更新日期：2015-01-01 00:00:00

abstract：:Release of phosphorothioate antisense oligonucleotides (PS-ASOs) from late endosomes (LEs) is a rate-limiting step and a poorly defined process for productive intracellular ASO drug delivery. Here, we examined the role of Golgi-endosome transport, specifically M6PR shuttling mediated by GCC2, in PS-ASO trafficking and...

journal_title：Nucleic acids research

authors： Liang XH,Sun H,Hsu CW,Nichols JG,Vickers TA,De Hoyos CL,Crooke ST

更新日期：2020-02-20 00:00:00

abstract：:The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...

journal_title：Nucleic acids research

authors： Zheng S,Shuman S,Schwer B

更新日期：2007-01-01 00:00:00

abstract：:Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...

journal_title：Nucleic acids research

authors： Han YH,Kim DK,Na TY,Ka NL,Choi HS,Lee MO

更新日期：2016-02-18 00:00:00

abstract：:Extensive efforts have been directed at the discovery, investigation and clinical monitoring of targeted therapeutics. These efforts may be facilitated by the convenient access of the genetic, proteomic, interactive and other aspects of the therapeutic targets. Here, we describe an update of the Therapeutic target dat...

journal_title：Nucleic acids research

authors： Li YH,Yu CY,Li XX,Zhang P,Tang J,Yang Q,Fu T,Zhang X,Cui X,Tu G,Zhang Y,Li S,Yang F,Sun Q,Qin C,Zeng X,Chen Z,Chen YZ,Zhu F

更新日期：2018-01-04 00:00:00

abstract：:Maintenance of stem-cell identity requires proper regulation of enhancer activity. Both transcription factors OCT4/SOX2/NANOG and histone methyltransferase complexes MLL/SET1 were shown to regulate enhancer activity, but how they are regulated in embryonic stem cells (ESCs) remains further studies. Here, we report a t...

journal_title：Nucleic acids research

authors： Niu C,Wang S,Guo J,Wei X,Jia M,Chen Z,Gong W,Qin Y,Wang X,Zhi X,Lu M,Chen S,Gu M,Zhang J,Han JJ,Lan F,Meng D

更新日期：2021-01-27 00:00:00

abstract：:The replication origin region of the Escherichia coli K-12 chromosome has been cloned, and a region of 245 base-pairs has been shown to contain all the information for autonomous replication (defined ori). In order to obtain further information on the sequence organization in the defined ori stretch, various types of ...

journal_title：Nucleic acids research

authors： Asada K,Sugimoto K,Oka A,Takanami M,Hirota Y

更新日期：1982-06-25 00:00:00

abstract：:The 5' portions and flanking sequences of genes encoding types 1, 12, 24, and 6 M proteins were compared. Although the DNA sequences encoding the amino-termini of the mature M proteins had no obvious similarity, upstream sequences, and those encoding the signal peptides (leader sequences) of the four M protein genes h...

journal_title：Nucleic acids research

authors： Haanes-Fritz E,Kraus W,Burdett V,Dale JB,Beachey EH,Cleary P

更新日期：1988-05-25 00:00:00

abstract：:We describe a new, broadly applicable methodology for screening in parallel interactions of RNA-binding proteins (RBPs) with large numbers of microRNA (miRNA) precursors and for determining their affinities in native form in the presence of cellular factors. The assays aim at identifying pre-miRNAs that are potentiall...

journal_title：Nucleic acids research

authors： Towbin H,Wenter P,Guennewig B,Imig J,Zagalak JA,Gerber AP,Hall J

更新日期：2013-02-01 00:00:00