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Human repair gene restores normal pattern of preferential DNA repair in repair defective CHO cells.

Abstract：

:The pattern of preferential DNA repair of UV-induced pyrimidine dimers was studied in repair-deficient Chinese hamster ovary (CHO) cells transfected with the human excision repair gene, ERCC-1. Repair efficiency was measured in the active dihydrofolate reductase (DHFR) gene and in its flanking, non-transcribed sequences in three cell lines: Wild type CHO cells, a UV-sensitive excision deficient CHO mutant, and the transfected line of the mutant carrying the expressed ERCC-1 gene. The CHO cells transformed with the human ERCC-1 gene repaired the active DHFR gene much more efficiently than the non-transcribed sequences, a pattern similar to that seen in wild type CHO cells. This pattern differs from that previously reported in CHO cells transfected with the denV gene of bacteriophage T4, in which both active and non-transcribed DNA sequences were efficiently repaired (Bohr and Hanawalt, Carcinogenesis 8: 1333-1336, 1987). The ERCC-1 gene product may specifically substitute for the repair enzyme present in normal hamster cells while the denV product, T4 endonuclease V, does not be appear to be constrained in its access to inactive chromatin.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Bohr VA,Chu EH,van Duin M,Hanawalt PC,Okumoto DS

doi

10.1093/nar/16.15.7397

subject

Has Abstract

pub_date

1988-08-11 00:00:00

pages

7397-403

issue

15

eissn

0305-1048

issn

1362-4962

journal_volume

16

pub_type

杂志文章

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