Messenger RNA abundance and lifetime: a correlation in Drosophila cells but not in HeLa.

abstract：:The photostability and narrow emission spectra of non-organic quantum dot fluorophores (QDs) make them desirable candidates for fluorescent in situ hybridization (FISH) to study the expression of specific mRNA transcripts. We developed a novel method for direct QD labeling of modified oligonucleotide probes through st...

journal_title：Nucleic acids research

authors： Chan P,Yuen T,Ruf F,Gonzalez-Maeso J,Sealfon SC

更新日期：2005-10-13 00:00:00

abstract：:The nucleotide sequence of Neurospora crassa 5.8 S rDNA and adjacent regions has been determined. The deduced 5.8 S rRNA sequence of Neurospora differs from the 5.8 S rRNA sequence of Saccharomyces cerevisiae at 13 of 158 residues. Nine of these differences are clustered in a segment capable of forming a short hairpin...

journal_title：Nucleic acids research

authors： Selker E,Yanofsky C

更新日期：1979-06-11 00:00:00

abstract：:Previously we identified Rrp1 and Rrp2 as two proteins required for the Sfr1/Swi5-dependent branch of homologous recombination (HR) in Schizosaccharomyces pombe. Here we use a yeast two-hybrid approach to demonstrate that Rrp1 and Rrp2 can interact with each other and with Swi5, an HR mediator protein. Rrp1 and Rrp2 f...

journal_title：Nucleic acids research

authors： Dziadkowiec D,Kramarz K,Kanik K,Wisniewski P,Carr AM

更新日期：2013-09-01 00:00:00

abstract：:The UAS (upstream activator sequence) of the yeast PGK gene contains a transcriptional activator domain located between bases -479 and -402 upstream from the initiating ATG. This region of the UAS contains three direct repeats of the sequence 5'CTTCC3'. The roles in transcriptional activation of these repeats and othe...

journal_title：Nucleic acids research

authors： Chambers A,Stanway C,Kingsman AJ,Kingsman SM

更新日期：1988-09-12 00:00:00

abstract：:We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Repl...

journal_title：Nucleic acids research

authors： Edenberg HJ,Waqar MA,Huberman JA

更新日期：1977-09-01 00:00:00

abstract：:The interaction of RNase T1 with calf thymus DNA was studied using uv difference spectroscopy and the effect of the enzyme on DNA melting. There was no indication of RNase T1 binding with native DNA. A prominent difference spectrum for RNase T1 binding with denatured DNA (d-DNA) was observed at pH 5, 25 degrees and lo...

journal_title：Nucleic acids research

authors： Walz FG,Biddlecome S,Hooverman L

更新日期：1975-01-01 00:00:00

abstract：:A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...

journal_title：Nucleic acids research

authors： Karataev GI,Permogorov VI,Vologodskii AV,Frank-Kamenetskii MD

更新日期：1978-07-01 00:00:00

abstract：:Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type cl...

journal_title：Nucleic acids research

authors： Deacon NJ,Shine J,Naora H

更新日期：1980-03-25 00:00:00

abstract：:The precursor to the Euglena light harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) is a polyprotein containing multiple copies of LHCPII covalently joined by a decapeptide linker. cDNA and genomic clones encoding the 5' and 3' end of a 6.6 kb LHCPII mRNA were sequenced. A 3.1 kb genomic region en...

journal_title：Nucleic acids research

authors： Muchhal US,Schwartzbach SD

更新日期：1994-12-25 00:00:00

abstract：:Gene trap vectors developed for genome-wide mutagenesis can be used to study factors governing the expression of exons inserted throughout the genome. For example, entrapment vectors consisting of a partial 3'-terminal exon [i.e. a neomycin resistance gene (Neo), a poly(A) site, but no 3' splice site] were typically e...

journal_title：Nucleic acids research

authors： Osipovich AB,White-Grindley EK,Hicks GG,Roshon MJ,Shaffer C,Moore JH,Ruley HE

更新日期：2004-05-20 00:00:00

abstract：:Trinucleotide repeat (TNR) expansions cause at least 17 heritable neurological diseases, including Huntington's disease. Expansions are thought to arise from abnormal processing of TNR DNA by specific trans-acting proteins. For example, the DNA repair complex MutSβ (MSH2-MSH3 heterodimer) is required in mice for on-go...

journal_title：Nucleic acids research

authors： Gannon AM,Frizzell A,Healy E,Lahue RS

更新日期：2012-11-01 00:00:00

abstract：:Single-stranded mRNA molecules form secondary structures through complementary self-interactions. Several hypotheses have been proposed on the relationship between the nucleotide sequence, encoded amino acid sequence and mRNA secondary structure. We performed the first transcriptome-wide in silico analysis of the huma...

journal_title：Nucleic acids research

authors： Shabalina SA,Ogurtsov AY,Spiridonov NA

更新日期：2006-05-08 00:00:00

abstract：:Ribosomal protein (RP) genes must be coordinately expressed for proper assembly of the ribosome yet the mechanisms that control expression of RP genes in metazoans are poorly understood. Recently, TATA-binding protein-related factor 2 (TRF2) rather than the TATA-binding protein (TBP) was found to function in transcrip...

journal_title：Nucleic acids research

authors： Baumann DG,Gilmour DS

更新日期：2017-10-13 00:00:00

abstract：:Targeted differentiation of human induced pluripotent stem cells (hiPSCs) using only chemicals would have value-added clinical potential in the regeneration of complex cell types including cardiomyocytes. Despite the availability of several chemical inhibitors targeting proteins involved in signaling pathways, no bioa...

journal_title：Nucleic acids research

authors： Taniguchi J,Pandian GN,Hidaka T,Hashiya K,Bando T,Kim KK,Sugiyama H

更新日期：2017-09-19 00:00:00

abstract：:The polyadenylate binding protein 1 (PABPN1) is a ubiquitously expressed RNA binding protein vital for multiple steps in RNA metabolism. Although PABPN1 plays a critical role in the regulation of RNA processing, mutation of the gene encoding this ubiquitously expressed RNA binding protein causes a specific form of mus...

journal_title：Nucleic acids research

authors： Banerjee A,Vest KE,Pavlath GK,Corbett AH

更新日期：2017-10-13 00:00:00

abstract：:A tetradecanucleotide U-A-G-C(U-C-G)2G-G-C-Up corresponding to bases 21-34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of chemically synthesized oligonucleotides: 1) U-A-G-C + U-C-G-U-C-G + G-G-C-Up and 2) U-A-G-C + U-C-G-U + C-G-G-G-C-Up. In reactio...

journal_title：Nucleic acids research

authors： Ohtsuka E,Doi T,Uemura H,Taniyama Y,Ikehara M

更新日期：1980-09-11 00:00:00

abstract：:The highly charged DNA chain may be either in an extended conformation, the coil, or condensed into a highly dense and ordered structure, the toroid. The transition, also called collapse of the chain, can be triggered in different ways, for example by changing the ionic conditions of the solution. We observe individua...

journal_title：Nucleic acids research

authors： Sung B,Leforestier A,Livolant F

更新日期：2016-02-18 00:00:00

abstract：:Conventional chemotherapy remains the primary treatment option for triple-negative breast cancer (TNBC). However, the current chemotherapeutic drugs have limited effects on TNBC, and often lead to serious side effects as well as drug resistance. Thus, more effective therapeutic options are sorely needed. As c-MYC onco...

journal_title：Nucleic acids research

authors： Hu MH,Wu TY,Huang Q,Jin G

更新日期：2019-11-18 00:00:00

abstract：:The database of Clusters of Orthologous Groups of proteins (COGs), which represents an attempt on a phylogenetic classification of the proteins encoded in complete genomes, currently consists of 2791 COGs including 45 350 proteins from 30 genomes of bacteria, archaea and the yeast Saccharomyces cerevisiae (http://www....

journal_title：Nucleic acids research

authors： Tatusov RL,Natale DA,Garkavtsev IV,Tatusova TA,Shankavaram UT,Rao BS,Kiryutin B,Galperin MY,Fedorova ND,Koonin EV

更新日期：2001-01-01 00:00:00

abstract：:Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...

journal_title：Nucleic acids research

authors： Sinclair JH,Bryant LA

更新日期：1987-11-25 00:00:00

abstract：:Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNA-like (C3'-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself. We performed molecular dynamics simulations on five isosequential duplexes (LNA-DNA, LNA-LNA, LNA-R...

journal_title：Nucleic acids research

authors： Pande V,Nilsson L

更新日期：2008-03-01 00:00:00

abstract：:After ionising radiation double-strand breaks (dsb) are lethal if not repaired or misrepaired. Cell killing is greatly enhanced by hyperthermia and it is questioned here whether heat not only affects dsb repair capacity but also fidelity in a chromosomal context. dsb repair experiments were designed so as to mainly sc...

journal_title：Nucleic acids research

authors： El-Awady RA,Dikomey E,Dahm-Daphi J

更新日期：2001-05-01 00:00:00

abstract：:We have previously described a partial Drosophila cDNA, clone P19, which bears homology to members of the RNA recognition motif (RRM) family of proteins [Haynes et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 1819-1823]. RNA binding as well as involvement in RNA processing has been demonstrated for some RRM proteins. We...

journal_title：Nucleic acids research

authors： Stolow DT,Haynes SR

更新日期：1995-03-11 00:00:00

abstract：:ArrayOme is a new program that calculates the size of genomes represented by microarray-based probes and facilitates recognition of key bacterial strains carrying large numbers of novel genes. Protein-coding sequences (CDS) that are contiguous on annotated reference templates and classified as 'Present' in the test st...

journal_title：Nucleic acids research

authors： Ou HY,Smith R,Lucchini S,Hinton J,Chaudhuri RR,Pallen M,Barer MR,Rajakumar K

更新日期：2005-01-07 00:00:00

abstract：:In diverse bacterial species, the turnover and processing of many RNAs is mediated by the ribonuclease RNase J, a member of the widely occurring metallo-β-lactamase enzyme family. We present crystal structures of Streptomyces coelicolor RNase J with bound RNA in pre- and post-cleavage states, at 2.27 Å and 2.80 Å reso...

journal_title：Nucleic acids research

authors： Pei XY,Bralley P,Jones GH,Luisi BF

更新日期：2015-09-18 00:00:00

abstract：:We have isolated the poly(A) polymerase (PAP) encoding gene pla1 [for poly(A) polymerase] from the fission yeast Schizosaccharomyces pombe. Protein sequence alignments with other poly(A) polymerases reveal that pla1 is more closely related to Saccharomyces cerevisiae PAP than to bovine PAP. The two yeast poly(A) polym...

journal_title：Nucleic acids research

authors： Ohnacker M,Minvielle-Sebastia L,Keller W

更新日期：1996-07-01 00:00:00

abstract：:Multivalent cations are known to condense DNA into higher ordered structures, including toroids and rods. Here we report that solid supports treated with monovalent or multivalent cationic silanes, followed by removal of soluble molecules, can condense DNA. The mechanism of this surface-directed condensation depends o...

journal_title：Nucleic acids research

authors： Fang Y,Hoh JH

更新日期：1998-01-15 00:00:00

abstract：:Partial restriction digestion is used to map restriction sites and the location of genes within yeast artificial chromosomes (YACs). Locus-specific probes are hybridised to the partially digested YAC DNA and the fragments to which they hybridise are compared with the pattern of partial digestion products that include ...

journal_title：Nucleic acids research

authors： Shields DC,Butler A,Mosurski KR,Walsh MT,Whitehead AS

更新日期：1996-11-15 00:00:00

abstract：:The cis-requirements for the first editing site in the atp9 mRNA from pea mitochondria were investigated in an in vitro RNA editing system. Template RNAs deleted 5' of -20 are edited correctly, but with decreased efficiency. Deletions between -20 and the edited nucleotide abolish editing activity. Substitution of the ...

journal_title：Nucleic acids research

authors： Takenaka M,Neuwirt J,Brennicke A

更新日期：2004-08-04 00:00:00

abstract：:Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. The CORUM (http://mips.gsf.de/genre/proj/corum/index.html) database is a collection of experimentally verified mammalian protein complexes. Information is manually derived by critical reading of the scient...

journal_title：Nucleic acids research

authors： Ruepp A,Brauner B,Dunger-Kaltenbach I,Frishman G,Montrone C,Stransky M,Waegele B,Schmidt T,Doudieu ON,Stümpflen V,Mewes HW

更新日期：2008-01-01 00:00:00