Database resources of the National Center for Biotechnology Information.

abstract：:Sequences preceding the minimal promoter play a role in the differential expression of the Xenopus somatic and oocyte-type 5S RNA genes. In this report, the somatic sequences between -32 and +37 are shown to increase transcriptional activity in microinjected embryos, yet have little to no effect in microinjected oocyt...

journal_title：Nucleic acids research

authors： Reynolds WF

更新日期：1989-11-25 00:00:00

abstract：:Mammalian mitochondrial ribosomes evolved from bacterial ribosomes by reduction of ribosomal RNAs, increase of ribosomal protein content, and loss of guanine nucleotides. Guanine is the base most sensitive to oxidative damage. By systematically comparing high-quality, small ribosomal subunit RNA sequence alignments an...

journal_title：Nucleic acids research

authors： Hosseini M,Roy P,Sissler M,Zirbel CL,Westhof E,Leontis N

更新日期：2018-11-16 00:00:00

abstract：:Xenbase (www.xenbase.org), the model organism database for Xenopus laevis and X. (Silurana) tropicalis, is the principal centralized resource of genomic, development data and community information for Xenopus research. Recent improvements include the addition of the literature and interaction tabs to gene catalog page...

journal_title：Nucleic acids research

authors： Bowes JB,Snyder KA,Segerdell E,Jarabek CJ,Azam K,Zorn AM,Vize PD

更新日期：2010-01-01 00:00:00

abstract：:A filter binding assay was developed that allows measurement of specific binding of trp repressor to operator DNA. The most important feature of this procedure is the concentration and type of salt present in the binding buffer. Using this assay the dissociation constant of the repressor-operator complex was determine...

journal_title：Nucleic acids research

authors： Klig LS,Crawford IP,Yanofsky C

更新日期：1987-07-10 00:00:00

abstract：:Regulation of gene expression influences almost all biological processes in an organism; sequence-specific DNA-binding transcription factors are critical to this control. For most genomes, the repertoire of transcription factors is only partially known. Hitherto transcription factor identification has been largely bas...

journal_title：Nucleic acids research

authors： Kummerfeld SK,Teichmann SA

更新日期：2006-01-01 00:00:00

abstract：:Computational modelling has become increasingly common in life science research. To provide a platform to support universal sharing, easy accessibility and model reproducibility, BioModels (https://www.ebi.ac.uk/biomodels/), a repository for mathematical models, was established in 2005. The current BioModels platform ...

journal_title：Nucleic acids research

authors： Malik-Sheriff RS,Glont M,Nguyen TVN,Tiwari K,Roberts MG,Xavier A,Vu MT,Men J,Maire M,Kananathan S,Fairbanks EL,Meyer JP,Arankalle C,Varusai TM,Knight-Schrijver V,Li L,Dueñas-Roca C,Dass G,Keating SM,Park YM,Buso N

更新日期：2020-01-08 00:00:00

abstract：:The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational effi...

journal_title：Nucleic acids research

authors： Gallie DR,Feder JN,Schimke RT,Walbot V

更新日期：1991-09-25 00:00:00

abstract：:The regions of the polypeptide chain immediately preceding or following an α-helix are known as Nt- and Ct cappings, respectively. Cappings play a central role stabilizing α-helices due to lack of intrahelical hydrogen bonds in the first and last turn. Sequence patterns of amino acid type preferences have been derived...

journal_title：Nucleic acids research

authors： Segura J,Oliva B,Fernandez-Fuentes N

更新日期：2012-01-01 00:00:00

abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...

journal_title：Nucleic acids research

authors： Nagashima T,Matsuda H,Silva DG,Petrovsky N,Konagaya A,Schönbach C,Kasukawa T,Arakawa T,Carninci P,Kawai J,Hayashizaki Y,RIKEN GER Group.,Genome Science Laoratory Members.

更新日期：2004-01-01 00:00:00

abstract：:Symmetry considerations of the kind of structures which can be generated when dimers of histones f2al-f3 and f2a2-f2b interact lead to the following conclusions: chromatin subunits based on closed-shell structures give rise to discrete, non-interacting nucleoprotein subunits with the histones arranged at random along ...

journal_title：Nucleic acids research

authors： Hyde JE,Walker IO

更新日期：1975-03-01 00:00:00

abstract：:Gene expression circuitries, which enable cells to detect precise levels within a morphogen concentration gradient, have a pivotal impact on biological processes such as embryonic pattern formation, paracrine and autocrine signalling, and cellular migration. We present the rational synthesis of a synthetic genetic cir...

journal_title：Nucleic acids research

authors： Greber D,Fussenegger M

更新日期：2010-10-01 00:00:00

abstract：:Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA. The three component substrate is characterized by single-stranded DNA target, an oligonucleotide probe, separated from a helper oligonucleotide by a one base g...

journal_title：Nucleic acids research

authors： Kutyavin IV,Milesi D,Belousov Y,Podyminogin M,Vorobiev A,Gorn V,Lukhtanov EA,Vermeulen NM,Mahoney W

更新日期：2006-01-01 00:00:00

abstract：:Analysis of the human VK (ref. 2) gene locus led to the detection of a new sequence family (L sequences). Its copy number is in the range of 10(2). The L sequences, which are about 500 bp long, are found as part of the 3' flanking regions of a clustered set of human VKI genes but they occur also separate from the gene...

journal_title：Nucleic acids research

authors： Straubinger B,Pech M,Mühlebach K,Jaenichen HR,Bauer HG,Zachau HG

更新日期：1984-07-11 00:00:00

abstract：:eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells to identify mRNAs i...

journal_title：Nucleic acids research

authors： Cridge AG,Castelli LM,Smirnova JB,Selley JN,Rowe W,Hubbard SJ,McCarthy JE,Ashe MP,Grant CM,Pavitt GD

更新日期：2010-12-01 00:00:00

abstract：:The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5' to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DN...

journal_title：Nucleic acids research

authors： Waltz SE,Trivedi AA,Leffak M

更新日期：1996-05-15 00:00:00

abstract：:Cultured mammalian cells transduced with the Escherichia coli gene, Ecogpt, synthesize the bacterial enzyme xanthine-guanine phosphoribosyl transferase (XGPT) (1). This paper describes a method for measuring XGPT activity in crude cell extracts by following the conversion of 14C-xanthine (X) to 14C-xanthine monophosph...

journal_title：Nucleic acids research

authors： Chu G,Berg P

更新日期：1985-04-25 00:00:00

abstract：:A 2.1-kb cDNA clone representing a new member of the protease inhibitor family was isolated from a human liver cDNA library. The inhibitor, named human Leuserpin 2 (hLS2), comprises 480 amino acids and contains a leucine residue at its putative reactive center. HLS2 is about 25-28% homologous to three human members of...

journal_title：Nucleic acids research

authors： Ragg H

更新日期：1986-01-24 00:00:00

abstract：:PKR is a doubled-stranded RNA-dependent protein kinase which is implicated in the regulation of several cellular processes, including cell proliferation. PKR undergoes phosphorylation and activation in mouse embryonic 3T3-F442A cells in response to endogenous RNA(s). Activation of PKR is related to growth and differen...

journal_title：Nucleic acids research

authors： Petryshyn RA,Ferrenz AG,Li J

更新日期：1997-07-01 00:00:00

abstract：:The widespread applications of various 'omics' technologies in biomedical research together with the emergence of public data repositories have resulted in a plethora of data sets for almost any given physiological state or disease condition. Properly combining or integrating these data sets with similar basic hypothe...

journal_title：Nucleic acids research

authors： Xia J,Fjell CD,Mayer ML,Pena OM,Wishart DS,Hancock RE

更新日期：2013-07-01 00:00:00

abstract：:Tcf/Lef family transcription factors are the downstream effectors of the Wingless/Wnt signal transduction pathway. Upon Wingless/Wnt signalling, beta-catenin translocates to the nucleus, interacts with Tcf (1-3) and thus activates transcription of target genes (4,5). Tcf factors also interact with members of the Grouc...

journal_title：Nucleic acids research

authors： Brantjes H,Roose J,van De Wetering M,Clevers H

更新日期：2001-04-01 00:00:00

abstract：:The number of known alternative human isoforms has been increasing steadily with the amount of available transcription data. To date, over 100 000 isoforms have been detected in EST libraries, and at least 75% of human genes have at least one alternative isoform. In this paper, we propose that most alternative splicin...

journal_title：Nucleic acids research

authors： Melamud E,Moult J

更新日期：2009-08-01 00:00:00

abstract：:cDNA expression libraries displayed on lambda phage have been successfully employed to identify partners involved in antibody-antigen, protein- protein and DNA-protein interactions and represent a novel approach to functional genomics. However, as in all other cDNA expression libraries based on fusion to a carrier pol...

journal_title：Nucleic acids research

authors： Ansuini H,Cicchini C,Nicosia A,Tripodi M,Cortese R,Luzzago A

更新日期：2002-08-01 00:00:00

abstract：:The yeast retrotransposon Ty encodes proteins that assemble into virus-like particles (Ty-VLPs) which can be readily purified. We have recently shown that expression of the pl protein encoded by the TYA gene of Ty is sufficient for particle formation. In this paper we show that when a heterologous coding sequence, hum...

journal_title：Nucleic acids research

authors： Malim MH,Adams SE,Gull K,Kingsman AJ,Kingsman SM

更新日期：1987-09-25 00:00:00

abstract：:Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2...

journal_title：Nucleic acids research

authors： Nguyen Q,Iritani A,Ohkita S,Vu BV,Yokoya K,Matsubara A,Ikeda KI,Suzuki N,Nakayashiki H

更新日期：2018-03-16 00:00:00

abstract：:DynOmics (dynomics.pitt.edu) is a portal developed to leverage rapidly growing structural proteomics data by efficiently and accurately evaluating the dynamics of structurally resolved systems, from individual molecules to large complexes and assemblies, in the context of their physiological environment. At the core o...

journal_title：Nucleic acids research

authors： Li H,Chang YY,Lee JY,Bahar I,Yang LW

更新日期：2017-07-03 00:00:00

abstract：:The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or...

journal_title：Nucleic acids research

authors： Kondo J,Westhof E

更新日期：2008-05-01 00:00:00

abstract：:A peptide initiation factor purified from rat liver and promoting the binding of initiator tRNA and model initiators to 40S and 80S ribosome at an acid pH liberates methionine and N-acetylmethionine from Trna Met f at neutral reaction. Phenylalanyl-tRNA, N-acetylphenylalanyl-tRNA and methionyl-tRNA Met m are not hydro...

journal_title：Nucleic acids research

authors： Hradec J

更新日期：1975-11-01 00:00:00

abstract：:Cationic aromatic tricyclic compounds including triphenylmethane dyes, phenazines, phenoxazines, acridines, phenothiazines, phenanthridinium compounds, anthracenes and xanthene dyes, which amplify cell killing in phleomycin-treated Escherichia coli B cells also modified phleomycin-induced breakdown of DNA to acid-solu...

journal_title：Nucleic acids research

authors： Grigg GW,Gero AM,Sasse WH,Sleigh MJ

更新日期：1984-12-11 00:00:00

abstract：:The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spac...

journal_title：Nucleic acids research

authors： Jordan BR,Latil-Damotte M,Jourdan R

更新日期：1980-08-25 00:00:00

abstract：:Synchronous plasmodia of cycloheximide-sensitive and cycloheximide-resistant strains of Physarum polycephalum were labelled with 3[H]-deoxyadenosine in pulse and pulse-chase experiments in presence and absence of cycloheximide. The replication products were studied with alkaline sucrose gradient sedimentation analysis...

journal_title：Nucleic acids research

authors： Funderud S,Haugli F

更新日期：1977-02-01 00:00:00