IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

abstract：:We characterize a component of the E. coli bacterial nucleoid H1a, which accumulates in stationary phase. This protein, identical with the major component of a plasmid-protein complex previously isolated in our laboratory, has a pI close to 7.5. Acrylamide gel electrophoresis and sedimentation in sucrose gradient have...

journal_title：Nucleic acids research

authors： Spassky A,Rimsky S,Garreau H,Buc H

更新日期：1984-07-11 00:00:00

abstract：:In the filamentous cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating the FurA-regulatory network, the number of target genes described for this essential transcription factor is limited to a handf...

journal_title：Nucleic acids research

authors： González A,Angarica VE,Sancho J,Fillat MF

更新日期：2014-04-01 00:00:00

abstract：:PRINTS is a database of protein family 'fingerprints' offering a diagnostic resource for newly-determined sequences. By contrast with PROSITE, which uses single consensus expressions to characterise particular families, PRINTS exploits groups of motifs to build characteristic signatures. These signatures offer improve...

journal_title：Nucleic acids research

authors： Attwood TK,Beck ME,Flower DR,Scordis P,Selley JN

更新日期：1998-01-01 00:00:00

abstract：:Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...

journal_title：Nucleic acids research

authors： Harkins KM,Schaefer NK,Troll CJ,Rao V,Kapp J,Naughton C,Shapiro B,Green RE

更新日期：2020-05-07 00:00:00

abstract：:7SK snRNA, an abundant RNA discovered in human nucleus, regulates transcription by RNA polymerase II (RNAPII). It sequesters and inhibits the transcription elongation factor P-TEFb which, by phosphorylation of RNAPII, switches transcription from initiation to processive elongation and relieves pauses of transcription....

journal_title：Nucleic acids research

authors： Lebars I,Martinez-Zapien D,Durand A,Coutant J,Kieffer B,Dock-Bregeon AC

更新日期：2010-11-01 00:00:00

abstract：:Cell differentiation is a central process in development and in cancer growth and dissemination. OCT4 (POU5F1) and NANOG are essential for cell stemness and pluripotency; yet, the mechanisms that regulate their expression remain largely unknown. Repetitive elements account for almost half of the Human Genome; still, t...

journal_title：Nucleic acids research

authors： Morales-Hernández A,González-Rico FJ,Román AC,Rico-Leo E,Alvarez-Barrientos A,Sánchez L,Macia Á,Heras SR,García-Pérez JL,Merino JM,Fernández-Salguero PM

更新日期：2016-06-02 00:00:00

abstract：:The DNA-binding specificity and affinity of the dimeric human transcription factor (TF) STAT1, were assessed by total internal reflectance fluorescence protein-binding microarrays (TIRF-PBM) to evaluate the effects of protein phosphorylation, higher-order polymerization and small-molecule inhibition. Active, phosphory...

journal_title：Nucleic acids research

authors： Bonham AJ,Wenta N,Osslund LM,Prussin AJ 2nd,Vinkemeier U,Reich NO

更新日期：2013-01-01 00:00:00

abstract：:In fragile X syndrome, hypermethylation of the expanded CGG repeat and of the upstream promoter leads to transcriptional silencing of the FMR1 gene. Absence of the FMR1 protein results in mental retardation. We previously proved that treatment with 5-azadeoxycytidine (5-azadC) of fragile X cell lines results in reacti...

journal_title：Nucleic acids research

authors： Pietrobono R,Pomponi MG,Tabolacci E,Oostra B,Chiurazzi P,Neri G

更新日期：2002-07-15 00:00:00

abstract：:Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-strande...

journal_title：Nucleic acids research

authors： Seidl CI,Lama L,Ryan K

更新日期：2013-02-01 00:00:00

abstract：:The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a t...

journal_title：Nucleic acids research

authors： Chrisey LA,Lee GU,O'Ferrall CE

更新日期：1996-08-01 00:00:00

abstract：:Complementary DNA clones encoding the human kidney epidermal growth factor (EGF) precursor have been isolated and sequenced. They predict the sequence of a 1,207 amino acid protein which contains EGF flanked by polypeptide segments of 970 and 184 residues at its NH2- and COOH-termini, respectively. The structural orga...

journal_title：Nucleic acids research

authors： Bell GI,Fong NM,Stempien MM,Wormsted MA,Caput D,Ku LL,Urdea MS,Rall LB,Sanchez-Pescador R

更新日期：1986-11-11 00:00:00

abstract：:The three-dimensional Poisson-Boltzmann equation for the distribution of counterion charge density around double helical DNA has been solved by an iterative procedure. These computations have been performed for 0.01M monovalent salt solutions. A systematic study of the ten possible sequences found among adjacent nucle...

journal_title：Nucleic acids research

authors： Pack GR,Wong L,Prasad CV

更新日期：1986-02-11 00:00:00

abstract：:Recent progress in predicting RNA structure is moving towards filling the 'gap' in 2D RNA structure prediction where, for example, predicted internal loops often form non-canonical base pairs. This is increasingly recognized with the steady increase of known RNA 3D modules. There is a general interest in matching stru...

journal_title：Nucleic acids research

authors： Theis C,Höner Zu Siederdissen C,Hofacker IL,Gorodkin J

更新日期：2013-12-01 00:00:00

abstract：:Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alteration...

journal_title：Nucleic acids research

authors： Gallie DR,Sleat DE,Watts JW,Turner PC,Wilson TM

更新日期：1988-02-11 00:00:00

abstract：:The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenoty...

journal_title：Nucleic acids research

authors： Triman KL

更新日期：1996-01-01 00:00:00

abstract：:Methylthiotransferases (MTTases) are a closely related family of proteins that perform both radical-S-adenosylmethionine (SAM) mediated sulfur insertion and SAM-dependent methylation to modify nucleic acid or protein targets with a methyl thioether group (-SCH(3)). Members of two of the four known subgroups of MTTases...

journal_title：Nucleic acids research

authors： Anton BP,Russell SP,Vertrees J,Kasif S,Raleigh EA,Limbach PA,Roberts RJ

更新日期：2010-10-01 00:00:00

abstract：:Prokaryotes evolved numerous systems that defend against predation by bacteriophages. In addition to well-known restriction-modification and CRISPR-Cas immunity systems, many poorly characterized systems exist. One class of such systems, named BREX, consists of a putative phosphatase, a methyltransferase and four othe...

journal_title：Nucleic acids research

authors： Gordeeva J,Morozova N,Sierro N,Isaev A,Sinkunas T,Tsvetkova K,Matlashov M,Truncaite L,Morgan RD,Ivanov NV,Siksnys V,Zeng L,Severinov K

更新日期：2019-01-10 00:00:00

abstract：:Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA h...

journal_title：Nucleic acids research

authors： Lin Z,Hung KN,Grollman AP,de los Santos C

更新日期：1998-05-15 00:00:00

abstract：:RNA-binding proteins (RBPs) play important roles in the post-transcriptional control of RNAs. Identifying RBP binding sites and characterizing RBP binding preferences are key steps toward understanding the basic mechanisms of the post-transcriptional gene regulation. Though numerous computational methods have been dev...

journal_title：Nucleic acids research

authors： Zhang S,Zhou J,Hu H,Gong H,Chen L,Cheng C,Zeng J

更新日期：2016-02-29 00:00:00

abstract：:The 3' splice site of the second intron (I2) of the human apolipoprotein-AII gene, (GT)16GGGCAG, is unique in that, although fully functional, a stretch of alternating guanine and thymine residues replaces the polypyrimidine tract usually associated with 3' splice junctions. The transient expression of successive 5' d...

journal_title：Nucleic acids research

authors： Shelley CS,Baralle FE

更新日期：1987-05-11 00:00:00

abstract：:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...

journal_title：Nucleic acids research

authors： Jacob ST,Rose KM

更新日期：1974-11-01 00:00:00

abstract：:With the abundant mammalian lncRNAs identified recently, a comprehensive annotation resource for these novel lncRNAs is an urgent need. Since its first release in November 2016, AnnoLnc has been the only online server for comprehensively annotating novel human lncRNAs on-the-fly. Here, with significant updates to mult...

journal_title：Nucleic acids research

authors： Ke L,Yang DC,Wang Y,Ding Y,Gao G

更新日期：2020-07-02 00:00:00

abstract：:Conventional approaches to predict transcriptional regulatory interactions usually rely on the definition of a shared motif sequence on the target genes of a transcription factor (TF). These efforts have been frustrated by the limited availability and accuracy of TF binding site motifs, usually represented as position...

journal_title：Nucleic acids research

authors： Elati M,Nicolle R,Junier I,Fernández D,Fekih R,Font J,Képès F

更新日期：2013-02-01 00:00:00

abstract：:The YaeJ protein is a codon-independent release factor with peptidyl-tRNA hydrolysis (PTH) activity, and functions as a stalled-ribosome rescue factor in Escherichia coli. To identify residues required for YaeJ function, we performed mutational analysis for in vitro PTH activity towards rescue of ribosomes stalled on ...

journal_title：Nucleic acids research

authors： Kogure H,Handa Y,Nagata M,Kanai N,Güntert P,Kubota K,Nameki N

更新日期：2014-03-01 00:00:00

abstract：:The Yeast Resource Center Public Data Repository (YRC PDR) serves as a single point of access for the experimental data produced from many collaborations typically studying Saccharomyces cerevisiae (baker's yeast). The experimental data include large amounts of mass spectrometry results from protein co-purification ex...

journal_title：Nucleic acids research

authors： Riffle M,Malmström L,Davis TN

更新日期：2005-01-01 00:00:00

abstract：:The side chains of the 20 types of amino acids, owing to a large extent to their different physical properties, have characteristic distributions in interior/surface regions of individual proteins and in interface/non-interface portions of protein surfaces that bind proteins or nucleic acids. These distributions have ...

journal_title：Nucleic acids research

authors： Tjong H,Qin S,Zhou HX

更新日期：2007-07-01 00:00:00

abstract：:We present a multi-laboratory effort to describe the structural and dynamical properties of duplex B-DNA under physiological conditions. By processing a large amount of atomistic molecular dynamics simulations, we determine the sequence-dependent structural properties of DNA as expressed in the equilibrium distributio...

journal_title：Nucleic acids research

authors： Dans PD,Balaceanu A,Pasi M,Patelli AS,Petkevičiūtė D,Walther J,Hospital A,Bayarri G,Lavery R,Maddocks JH,Orozco M

更新日期：2019-12-02 00:00:00

abstract：:Base excision repair (BER), which is initiated by DNA N-glycosylase proteins, is the frontline for repairing potentially mutagenic DNA base damage. The NTHL1 glycosylase, which excises DNA base damage caused by reactive oxygen species, is thought to be a tumor suppressor. However, in addition to NTHL1 loss-of-function...

journal_title：Nucleic acids research

authors： Limpose KL,Trego KS,Li Z,Leung SW,Sarker AH,Shah JA,Ramalingam SS,Werner EM,Dynan WS,Cooper PK,Corbett AH,Doetsch PW

更新日期：2018-05-18 00:00:00

abstract：:The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA...

journal_title：Nucleic acids research

authors： Andersen FF,Knudsen B,Oliveira CL,Frøhlich RF,Krüger D,Bungert J,Agbandje-McKenna M,McKenna R,Juul S,Veigaard C,Koch J,Rubinstein JL,Guldbrandtsen B,Hede MS,Karlsson G,Andersen AH,Pedersen JS,Knudsen BR

更新日期：2008-03-01 00:00:00

abstract：:Native nucleotides show a hyperbolic concentration dependence of the pre-steady-state rate of incorporation while maintaining concentration-independent amplitude due to fast, largely irreversible pyrophosphate release. The kinetics of 3'-azido-2',3'-dideoxythymidine (AZT) incorporation exhibit an increase in amplitude...

journal_title：Nucleic acids research

authors： Hanes JW,Johnson KA

更新日期：2007-01-01 00:00:00