Abstract:
:Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA heteroduplex containing a synthetic abasic site (tetrahydrofuran) residue positioned in a sequence that promotes one base deletions. Analysis of NMR spectra indicates that the stem region of the duplex adopts a right-handed helical structure and the glycosidic torsion angle is in anti orientation for all residues. NOE interactions establish Watson-Crick alignments for all canonical base pairs of the duplex. Measurement of distance interactions at the lesion site shows the abasic residue excluded from the helix. Restrained molecular dynamics simulations generated three-dimensional models in excellent agreement with the spectroscopic data. These structures show a regular duplex region and a slight bend at the lesion site. The tetrahydrofuran residue extrudes from the helix and is highly flexible. The model reported here, in conjunction with a previous study performed on abasic sites, explains the structural bias of one-base deletion mutations.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lin Z,Hung KN,Grollman AP,de los Santos Cdoi
10.1093/nar/26.10.2385subject
Has Abstractpub_date
1998-05-15 00:00:00pages
2385-91issue
10eissn
0305-1048issn
1362-4962pii
gkb413journal_volume
26pub_type
杂志文章abstract::Guanine-rich DNA sequences can form G-quadruplexes stabilized by stacked G-G-G-G tetrads in monovalent cation-containing solution. The length and number of individual G-tracts and the length and sequence context of linker residues define the diverse topologies adopted by G-quadruplexes. The review highlights recent so...
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更新日期:1992-12-11 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2007-01-01 00:00:00
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更新日期:1977-11-01 00:00:00
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