Abstract:
:We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome copy of M-MuLV in their germ line at different chromosomal positions and differ from each other with respect to expression of the proviral genome. We show here that the different M-MuLV proviral genome copies were transcribed into virus-specific RNA with similar efficiencies. Transcription of viral RNA initiated correctly at the viral promoter in the 5' LTR, whereas the promoter in the 3' LTR was used with a much lower frequency, if at all, for initiation of RNA synthesis. Most of the virus-specific transcripts were smaller than authentic M-MuLV mRNA and confined to the oocyte nucleus. Using immunoprecipitation, we were not able to detect virus-specific proteins after injection of proviral DNA, whereas after injection of a comparable amount of M-MuLV mRNA viral protein was readily detectable.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Breindl M,Kalthoff H,Jaenisch Rdoi
10.1093/nar/11.12.3989subject
Has Abstractpub_date
1983-06-25 00:00:00pages
3989-4006issue
12eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::TRF1, a duplex telomeric DNA-binding protein, plays an important role in telomere metabolism. We have previously reported that a fraction of endogenous TRF1 can stably exist free of telomere chromatin when it is phosphorylated at T371 by Cdk1; however, the role of this telomere-free (pT371)TRF1 has yet to be fully cha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt775
更新日期:2013-12-01 00:00:00
abstract::The anticancer drug cisplatin reacts with DNA leading to the formation of interstrand and intrastrand cross-links that are the critical cytotoxic lesions. In contrast to cells bearing mutations in other components of the nucleotide excision repair apparatus (XPB, XPD, XPG and CSB), cells defective for the ERCC1-XPF st...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf479
更新日期:2002-09-01 00:00:00
abstract::Fluoropyrimidines, such as 5-fluorouracil (5-FU), are used extensively in cancer therapy. In the cell, 5-FU is metabolized to 5-fluorodeoxyuridylate (5-FdUMP), a tight binding covalent inhibitor of thymidylate synthase (TS). In order to create 5-FdUMP resistant enzymes to protect chemosensitive normal cells and furthe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.18.3702
更新日期:1999-09-15 00:00:00
abstract::Four pseudorevertants of a -10 region lacP mutation were isolated. Three of these mutations were found to activate nascent promoters. These mutations were: a -2 G/C----A/T change (-2A) promoting transcription at position +11, a +1 A/T----T/A change (+1T) promoting transcription initiation at position +13, and a +10 C/...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.10.3927
更新日期:1989-05-25 00:00:00
abstract::The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.5.1539
更新日期:1977-01-01 00:00:00
abstract::DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.4.771
更新日期:1977-04-01 00:00:00
abstract::In the poly(A)+RNA isolated from the ciliate Paramecium primaurelia is found a discrete and abundant mRNA species of high molecular weight (corresponding to about 9,000 nucleotides). This mRNA species has size and abundance characteristics that identify it tentatively as the message coding for the variant cell-surface...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.21.7375
更新日期:1983-11-11 00:00:00
abstract::Mitochondrial DNA maintenance and segregation are dependent on the actin cytoskeleton in budding yeast. We found two cytoskeletal proteins among six proteins tightly associated with rat liver mitochondrial DNA: non-muscle myosin heavy chain IIA and β-actin. In human cells, transient gene silencing of MYH9 (encoding no...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr052
更新日期:2011-07-01 00:00:00
abstract::The current methods for quantifying genome-wide 5-methylcytosine (5mC) oxides are still scarce, mostly restricted with two limitations: assay sensitivity is seriously compromised with cost, assay time and sample input; epigenetic information is irreproducible during polymerase chain reaction (PCR) amplification withou...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz704
更新日期:2019-11-04 00:00:00
abstract::The human gene for plasminogen activator inhibitor type-1 (PAI-1) has been isolated and its promoter region characterized. PAI-1 regulation by glucocorticoids, transforming growth factor-beta (TGF-beta) and the phorbol ester PMA is shown to be exerted at the promoter level. A fragment spanning 805 nucleotides of the 5...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.7.2805
更新日期:1988-04-11 00:00:00
abstract::Prokaryotes use primed CRISPR adaptation to update their memory bank of spacers against invading genetic elements that have escaped CRISPR interference through mutations in their protospacer target site. We previously observed a trend that nucleotide-dependent mismatches between crRNA and the protospacer strongly infl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky687
更新日期:2018-11-02 00:00:00
abstract::Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5'-TTTN-3' protospacer adjacent motif (PAM) at the 5' end of the protospacer for genome editing. For widespread application in...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx783
更新日期:2017-11-02 00:00:00
abstract::Transcription factor binding sites (TFBSs) are short DNA sequences interacting with transcription factors (TFs), which regulate gene expression. Due to the relatively short length of such binding sites, it is largely unclear how the specificity of protein-DNA interaction is achieved. Here, we have performed a genome-w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl248
更新日期:2006-05-02 00:00:00
abstract::The potency of the immune response has still to be harnessed effectively to combat human cancers. However, the discovery of T-cell targets in melanomas and other tumors has raised the possibility that cancer vaccines can be used to induce a therapeutically effective immune response against cancer. The targets, cancer-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn673
更新日期:2009-01-01 00:00:00
abstract::G-quadruplexes (G4s) are non-canonical nucleic acids secondary structures that form within guanine-rich strands of regulatory genomic regions. G4s have been extensively described in the human genome, especially in telomeres and oncogene promoters; in recent years the presence of G4s in viruses has attracted increasing...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gky187
更新日期:2018-04-20 00:00:00
abstract::The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1337
更新日期:2016-01-04 00:00:00
abstract::The Superimposé webserver performs structural similarity searches with a preference towards 3D structure-based methods. Similarities can be detected between small molecules (e.g. drugs), parts of large structures (e.g. binding sites of proteins) and entire proteins. For this purpose, a number of algorithms were implem...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn285
更新日期:2008-07-01 00:00:00
abstract::A set of new sulfurizing agents representing disulfides of arylsulfonic acids has been developed for the automated synthesis of phosphorothioate oligonucleotide analogues via the phosphoramidite method. These reagents, such as bis(benzenesulfonyl)disulfide, bis(p-toluenesulfonyl)disulfide, bis(p-methoxybenzensulfonyl)...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.20.4029
更新日期:1995-10-25 00:00:00
abstract::Lateral ribosomal stalk is responsible for binding and recruiting translation factors during protein synthesis. The eukaryotic stalk consists of one P0 protein with two copies of P1•P2 heterodimers to form a P0(P1•P2)₂ pentameric P-complex. Here, we have solved the structure of full-length P1•P2 by nuclear magnetic re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt636
更新日期:2013-10-01 00:00:00
abstract::The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.20.6393
更新日期:1982-10-25 00:00:00
abstract::Accurate mapping of spliced RNA-Seq reads to genomic DNA has been known as a challenging problem. Despite significant efforts invested in developing efficient algorithms, with the human genome as a primary focus, the best solution is still not known. A recently introduced tool, TrueSight, has demonstrated better perfo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1141
更新日期:2014-02-01 00:00:00
abstract::This paper examines theoretically the effects that restraints on the tertiary structure of a superhelical DNA domain exert on the energetics of linking and the onset of conformational transitions. The most important tertiary constraint arises from the nucleosomal winding of genomic DNA in vivo. Conformational transiti...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/15.23.9985
更新日期:1987-12-10 00:00:00
abstract::Human prostatic acid phosphatase (PAcP) is a prostate epithelium-specific differentiation antigen. Cellular PAcP functions as a neutral protein tyrosine phosphatase and is involved in regulating androgen-promoted prostate cancer cell proliferation. Despite the fact that the promoter of the PAcP gene has been cloned, t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh677
更新日期:2004-07-07 00:00:00
abstract::An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its applicat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.9.2963
更新日期:1979-07-11 00:00:00
abstract::Murine erythroleukemic cells in culture accumulate cytoplasmic globin mRNA during differentiation induced by dimethyl sulfoxide (DMSO)1. Chromatin was prepared from DMSO induced erythroleukemic cells that were transcribing globin RNA in order to determine whether in vitro synthesis of globin RNA sequences was possible...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.1.275
更新日期:1979-01-01 00:00:00
abstract::The expression of thymidine kinase in fungi, which normally lack this enzyme, will greatly aid the study of DNA metabolism and provide useful drug-sensitive phenotypes. The herpes simplex virus type-1 thymidine kinase gene ( tk ) was expressed in Neurospora crassa. tk was expressed as a fusion to N.crassa arg-2 regula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.12.2389
更新日期:1997-06-15 00:00:00
abstract::To study the effect of intron size on splicing efficiency we have varied the size of the avian leukosis virus (ALV) env mRNA intron in a cloned ALV genome. This was accomplished by deletion of ALV sequences or insertion of phage lambda DNA. The effect of these modifications on splicing was analyzed by microinjection o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.19.6177
更新日期:1982-10-11 00:00:00
abstract::RNA-protein complexes are essential in mediating important fundamental cellular processes, such as transport and localization. In particular, ncRNA-protein interactions play an important role in post-transcriptional gene regulation like mRNA localization, mRNA stabilization, poly-adenylation, splicing and translation....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv020
更新日期:2015-02-18 00:00:00
abstract::In plants, nuclear multisubunit RNA polymerases IV and V are RNA Polymerase II-related enzymes that synthesize non-coding RNAs for RNA-directed DNA methylation (RdDM) and transcriptional gene silencing. Here, we tested the importance of the C-terminal domain (CTD) of Pol IV's largest subunit given that the Pol II CTD ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz615
更新日期:2019-09-26 00:00:00
abstract::Transactivation by the ETS family of transcription factors, whose members share structurally conserved DNA-binding domains, is variably sensitive to methylation of their target genes. The mechanism by which DNA methylation controls ETS proteins remains poorly understood. Uncertainly also pervades the effects of hemi-m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw528
更新日期:2016-10-14 00:00:00