Abstract：
:The chemical shifts of the well-resolved ring nitrogen protons of uracil and a series of substituted uracils, including pseudouridine (5-ribosyl-uracil) and uridine (1-ribosyl-uracil) were determined using nuclear magnetic resonance (NMR). The observed chemical shifts suggest the existence of an atypical syn conformation for pseudouridine in the Apsi base pair in regulatory tRNAs in solution.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hurd RE,Reid BRdoi
10.1093/nar/4.8.2747subject
Has Abstractpub_date
1977-08-01 00:00:00pages
2747-55issue
8eissn
0305-1048issn
1362-4962journal_volume
4pub_type
杂志文章abstract：:Loop length and its composition are important for the structural and functional versatility of quadruplexes. To date studies on the loops have mainly concerned model sequences compared with naturally occurring quadruplex sequences which have diverse loop lengths and compositions. Herein, we have characterized 36 quadr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn543
更新日期：2008-10-01 00:00:00
abstract：:The ability of the tumor suppressor protein, p53, to recognize certain types of DNA lesions may represent one of the mechanisms by which this protein modulates cellular response to DNA damage. p53 DNA binding properties are regulated by several factors, such as post-translational modifications including phosphorylatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.11.2427
更新日期：2002-06-01 00:00:00
abstract：:Zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) has long been known as a master transcriptional repressor of autophagy. Here, we identify a novel role for ZKSCAN3 in alleviating senescence that is independent of its autophagy-related activity. Downregulation of ZKSCAN3 is observed in aged human mesenchymal ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa425
更新日期：2020-06-19 00:00:00
abstract：:We present the sequences of five additional human U2 pseudogenes which are very similar to the U2.13 pseudogene reported previously [Van Arsdell et al. (1981) Cell 26, 11-17]. All six U2 pseudogenes preserve the 5' end of the mature U2 snRNA sequence, and all six are flanked by nearly perfect direct repeats that diffe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.3.1463
更新日期：1984-02-10 00:00:00
abstract：:Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process. We demonstrate its ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh009
更新日期：2004-01-02 00:00:00
abstract：:Structural information on interacting proteins is important for understanding life processes at the molecular level. Genome-wide docking database is an integrated resource for structural studies of protein-protein interactions on the genome scale, which combines the available experimental data with models obtained by ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp944
更新日期：2010-01-01 00:00:00
abstract：:We have developed efficient methods for creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of DNA mapping and sequencing. A novel plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial tran...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.18.3765
更新日期：1994-09-11 00:00:00
abstract：:The editing function of aminoacyl-tRNA synthetases (aaRSs) is indispensible for formation of the correct aminoacyl-tRNAs. Editing deficiency may lead to growth inhibition and the pathogenesis of various diseases. Herein, we confirmed that norvaline (Nva) but not isoleucine or valine is the major threat to the editing ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx487
更新日期：2017-07-07 00:00:00
abstract：:Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 phi2.5 promoter. Efficient fluorophore labeling of 5' RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni036
更新日期：2005-02-24 00:00:00
abstract：:Proteins are highly dynamic molecules, whose function is intrinsically linked to their molecular motions. Despite the pivotal role of protein dynamics, their computational simulation cost has led to most structure-based approaches for assessing the impact of mutations on protein structure and function relying upon sta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky300
更新日期：2018-07-02 00:00:00
abstract：:The single nucleotide polymorphisms (SNPs) in conserved protein regions have been thought to be strong candidates that alter protein functions. Thus, we have developed SNP@Domain, a web resource, to identify SNPs within human protein domains. We annotated SNPs from dbSNP with protein structure-based as well as sequenc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl323
更新日期：2006-07-01 00:00:00
abstract：:Efficient transcription from the human immunodeficiency virus (HIV) promoter depends on binding of the viral regulatory protein Tat to a cis-acting RNA regulatory element, TAR. Tat binds at a trinucleotide bulge located near the apex of the TAR stem-loop structure. An essential feature of Tat-TAR interaction is that t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.20.3974
更新日期：1996-10-15 00:00:00
abstract：:Analysis of haplotypes is an important tool in population genetics, familial heredity and gene mapping. Determination of haplotypes of multiple single nucleotide polymorphisms (SNPs) or other simple mutations is time consuming and expensive when analyzing large populations, and often requires the help of computational...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnf062
更新日期：2002-06-15 00:00:00
abstract：:The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.9.3357
更新日期：1978-09-01 00:00:00
abstract：:The mRNA export complex TREX (TREX) is known to contain Aly, UAP56, Tex1 and the THO complex, among which UAP56 is required for TREX assembly. Here, we systematically investigated the role of each human TREX component in TREX assembly and its association with the mRNA. We found that Tex1 is essentially a subunit of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1188
更新日期：2013-01-01 00:00:00
abstract：:We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated DNA. To study the effects of the phosphorylation/dephosphorylation of MDBP-2-H1 on the expression of the avian vitellogeni...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.10.1816
更新日期：1996-05-15 00:00:00
abstract：:Chromosomes in vertebrates are protected at both ends by telomere DNA composed of tandem (TTAGGG)n repeats. DNA replication produces a blunt-ended leading strand telomere and a lagging strand telomere carrying a single-stranded G-rich overhang at its end. The G-rich strand can form G-quadruplex structure in the presen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm203
更新日期：2007-01-01 00:00:00
abstract：:The DNA sequence of a 354 basepair EcoRI-HindIII fragment of plasmid pMB9 which has originally been derived from plasmid pSC101 has been resolved. This fragment contains a promoter for transcription directed towards the EcoRI site. Escherichia coli RNA polymerase binds to a region within the EcoRI-HindIII fragment whi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.7.1535
更新日期：1980-04-11 00:00:00
abstract：:LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally he...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.2.645
更新日期：1986-01-24 00:00:00
abstract：:MDg3 is a family of mobile dispersed genetic elements represented by 15 copies in the haploid genome of D. melanogaster and flanked, like other similar elements, by the regions of homology. In the present work, these regions of mdg3 have been sequenced. The existence of perfect direct repeats 268 base pairs long has b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.15.3263
更新日期：1980-08-11 00:00:00
abstract：:The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.10.1758
更新日期：1995-05-25 00:00:00
abstract：:We have used paired-end sequencing of yeast nucleosomal DNA to obtain accurate genomic maps of nucleosome positions and occupancies in control cells and cells treated with 3-aminotriazole (3AT), an inducer of the transcriptional activator Gcn4. In control cells, 3AT-inducible genes exhibit a series of distinct nucleos...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr643
更新日期：2011-12-01 00:00:00
abstract：:Primase synthesizes decaribonucleotides for priming of lagging and possibly leading strand synthesis at a replication fork. The sites of initiation by purified mouse primase were shown to be highly specific within the SV40 origin of replication. This study further examines the role of the 27-bp inverted repeat in the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.5.1953
更新日期：1989-03-11 00:00:00
abstract：:The cDNA encoding the human RNA lariat debranching enzyme (hDBR1) was identified and cloned by searching the Expressed Sequence Tag (EST) database and screening a HeLa cDNA library, based on predicted amino acid sequence homologies with the Saccharomyces cerevisiae, Schizosaccharomyces pombe and Caenorhabditis elegans...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.18.3666
更新日期：2000-09-15 00:00:00
abstract：:We propose a novel mechanism of gene regulation in Mycobacterium tuberculosis where the protein Rv1222 inhibits transcription by anchoring RNA polymerase (RNAP) onto DNA. In contrast to our existing knowledge that transcriptional repressors function either by binding to DNA at specific sequences or by binding to RNAP,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv516
更新日期：2015-07-13 00:00:00
abstract：:DNA glycosylases from the Fpg/Nei structural superfamily are base excision repair enzymes involved in the removal of a wide variety of mutagen and potentially lethal oxidized purines and pyrimidines. Although involved in genome stability, the recent discovery of synthetic lethal relationships between DNA glycosylases ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku613
更新日期：2014-01-01 00:00:00
abstract：:Understanding the evolutionary and genomic mechanisms responsible for turning the soil-derived saprophytic mycobacteria into lethal intracellular pathogens is a critical step towards the development of strategies for the control of mycobacterial diseases. In this context, Mycobacterium indicus pranii (MIP) is of speci...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks793
更新日期：2012-11-01 00:00:00
abstract：:Loss-of-function (LOF) methods such as RNA interference (RNAi), antisense oligonucleotides or CRISPR-based genome editing provide unparalleled power for studying the biological function of genes of interest. However, a major concern is non-specific targeting, which involves depletion of transcripts other than those in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky437
更新日期：2018-07-06 00:00:00
abstract：:In the haploid genome of Xenopus laevis there are two genes coding for the r-protein L14. It is not known if they are located on the same chromosome. cDNA clones deriving from the transcripts of the two genes have been isolated from an oocyte messenger cDNA bank showing that they are both expressed. We have studied th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.19.7633
更新日期：1986-10-10 00:00:00
abstract：:Several proteins that have substantially diverged during evolution retain similar three-dimensional structures and biological function inspite of poor sequence identity. The database on Genomic Distribution of protein structural domain Superfamilies (GenDiS) provides record for the distribution of 4001 protein domains...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki087
更新日期：2005-01-01 00:00:00
