The transposable element Mdg3 in Drosophila melanogaster is flanked with the perfect direct and mismatched inverted repeats.

abstract：:The interactions between beef tRNATrp with avian myeloblastosis reverse transcriptase have been studied by statistical chemical modifications of phosphate (ethylnitrosourea) and cytidine (dimethyl sulfate) residues, as well as by digestion of complexed tRNA by Cobra venom nuclease and Neurospora crassa endonuclease. R...

journal_title：Nucleic acids research

authors： Garret M,Romby P,Giegé R,Litvak S

更新日期：1984-03-12 00:00:00

abstract：:Ribosomal RNA (rRNA) in intact ribosomes is cleaved into discrete products on incubation of reticulocyte lysates or L-cell extracts with ppp(A2'p)3A. Cleavage of rRNA may, therefore, provide a useful assay for 2-5A (ppp)A2'p)nA; n = 2 to 4) or for the presence of a 2-5A-dependent nuclease. The results with reticulocyt...

journal_title：Nucleic acids research

authors： Wreschner DH,James TC,Silverman RH,Kerr IM

更新日期：1981-04-10 00:00:00

abstract：:We previously proposed that the first enzyme for histidine biosynthesis in Salmonellatyphimurium plays a role in regulating expression of the histidine operon and that in order to play this role the enzyme must form a complex with histidyl-tRNA. Among the many observations that led to these conclusions were 1) that re...

journal_title：Nucleic acids research

authors： Smith O,Meyers MM,Vogel T,Deeley RD,Goldberger R

更新日期：1974-07-01 00:00:00

abstract：:BRENDA (BRaunschweig ENzyme DAtabase) represents a comprehensive collection of enzyme and metabolic information, based on primary literature. The database contains data from at least 83,000 different enzymes from 9800 different organisms, classified in approximately 4200 EC numbers. BRENDA includes biochemical and mol...

journal_title：Nucleic acids research

authors： Schomburg I,Chang A,Ebeling C,Gremse M,Heldt C,Huhn G,Schomburg D

更新日期：2004-01-01 00:00:00

abstract：:For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a heterologous mammalian ge...

journal_title：Nucleic acids research

authors： Adachi N,Ikeda H,Kikuchi A

更新日期：1994-10-11 00:00:00

abstract：:A hallmark of defense mechanisms based on clustered regularly interspaced short palindromic repeats (CRISPR) and associated sequences (Cas) are the crRNAs that guide these complexes in the destruction of invading DNA or RNA. Three separate CRISPR-Cas systems exist in the cyanobacterium Synechocystis sp. PCC 6803. Base...

journal_title：Nucleic acids research

authors： Reimann V,Alkhnbashi OS,Saunders SJ,Scholz I,Hein S,Backofen R,Hess WR

更新日期：2017-01-25 00:00:00

abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...

journal_title：Nucleic acids research

authors： Hulla JE,Schneider RP

更新日期：1993-02-11 00:00:00

abstract：:DNA target recognizing domains of different multispecific DNA-cytosine-methyltransferases can be rearranged through engineering of the corresponding genes to generate enzymes with novel combinations of target recognition. ...

journal_title：Nucleic acids research

authors： Trautner TA,Balganesh TS,Pawlek B

更新日期：1988-07-25 00:00:00

abstract：:In the fission yeast Schizosaccharomyces pombe, the cdc11 gene is required for the initiation of septum formation at the end of mitosis. The sce3 gene was cloned as a multi-copy suppressor of the heat-sensitive mutant cdc11-136. When over-expressed, it rescues all mutants of cdc11 and also a heat-sensitive allele of c...

journal_title：Nucleic acids research

authors： Schmidt S,Hofmann K,Simanis V

更新日期：1997-09-01 00:00:00

abstract：:Several plasmids which direct the expression of human interferon-gamma (IFN-gamma) cDNA in the yeast Saccharomyces cerevisiae under the transcriptional control of the 3-phosphoglycerate kinase (PGK) promoter have been constructed. Up to 25 x 10(6) units per liter culture of biologically active IFN-gamma are synthesize...

journal_title：Nucleic acids research

authors： Derynck R,Singh A,Goeddel DV

更新日期：1983-03-25 00:00:00

abstract：:Transposable elements (TEs) comprise a large proportion of long non-coding RNAs (lncRNAs). Here, we employed CRISPR to delete a short interspersed nuclear element (SINE) in Malat1, a cancer-associated lncRNA, to investigate its significance in cellular physiology. We show that Malat1 with a SINE deletion forms diffuse...

journal_title：Nucleic acids research

authors： Nguyen TM,Kabotyanski EB,Reineke LC,Shao J,Xiong F,Lee JH,Dubrulle J,Johnson H,Stossi F,Tsoi PS,Choi KJ,Ellis AG,Zhao N,Cao J,Adewunmi O,Ferreon JC,Ferreon ACM,Neilson JR,Mancini MA,Chen X,Kim J,Ma L,Li W,Ro

更新日期：2020-03-18 00:00:00

abstract：:With the recent increase in the number of mammalian genomes being sequenced, large-scale genome scans for human-specific positive selection are now possible. Selection can be inferred through phylogenetic analysis by comparing the rates of silent and replacement substitution between related species. Maximum-likelihood...

journal_title：Nucleic acids research

authors： Nickel GC,Tefft D,Adams MD

更新日期：2008-01-01 00:00:00

abstract：:The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA...

journal_title：Nucleic acids research

authors： Andersen FF,Knudsen B,Oliveira CL,Frøhlich RF,Krüger D,Bungert J,Agbandje-McKenna M,McKenna R,Juul S,Veigaard C,Koch J,Rubinstein JL,Guldbrandtsen B,Hede MS,Karlsson G,Andersen AH,Pedersen JS,Knudsen BR

更新日期：2008-03-01 00:00:00

abstract：:A set of new sulfurizing agents representing disulfides of arylsulfonic acids has been developed for the automated synthesis of phosphorothioate oligonucleotide analogues via the phosphoramidite method. These reagents, such as bis(benzenesulfonyl)disulfide, bis(p-toluenesulfonyl)disulfide, bis(p-methoxybenzensulfonyl)...

journal_title：Nucleic acids research

authors： Efimov VA,Kalinkina AL,Chakhmakhcheva OG,Hill TS,Jayaraman K

更新日期：1995-10-25 00:00:00

abstract：:Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 phi2.5 promoter. Efficient fluorophore labeling of 5' RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current...

journal_title：Nucleic acids research

authors： Li N,Yu C,Huang F

更新日期：2005-02-24 00:00:00

abstract：:Two fragments cloned from purified human satellite III DNA do not cross-react with each other. One fragment, for which a partial sequence is reported, hybridises to satellite II as well as III and is shown to originate on chromosome 1. The other cloned fragment originates from the Y chromosome. This fragment has under...

journal_title：Nucleic acids research

authors： Cooke HJ,Hindley J

更新日期：1979-07-25 00:00:00

abstract：:These studies employed the synthetic linear DNA, poly dGdC, in the B and cobalt hexammine chloride (Co)-induced Z form to determine the effect of conformation on protein-DNA interactions. The rate of the reaction of the restriction endonucleases, Hha I and Cfo I, are reduced with Z DNA as compared to B DNA. The abilit...

journal_title：Nucleic acids research

authors： Soslau G,Parker J,Nelson JW

更新日期：1986-09-25 00:00:00

abstract：:Sodium is one of the most abundant metals in the environment and in biology, playing critical ecological and physiological roles. Na+ is also the most common buffer salt for nucleic acids research, while its specific interaction with DNA has yet to be fully studied. Herein, we probe a highly selective and robust Na+ a...

journal_title：Nucleic acids research

authors： Zhou W,Ding J,Liu J

更新日期：2016-12-01 00:00:00

abstract：:RBD-1 is the Caenorhabditis elegans homolog of Mrd1p, which was recently shown to be required for 18S ribosomal RNA (rRNA) processing in yeast. To gain insights into the relationship between ribosome biogenesis and the development of multicellular organisms, we examined the expression and function of RBD-1. Maternal R...

journal_title：Nucleic acids research

authors： Saijou E,Fujiwara T,Suzaki T,Inoue K,Sakamoto H

更新日期：2004-02-10 00:00:00

abstract：:A single base change in 16S rRNA (C726 to G) has previously been shown to have a dramatic effect on protein synthesis in E. coli (1). This paper more specifically details the effects of the mutation on mRNA binding and translation-termination. The in vitro technique of toeprinting (2) was used to demonstrate that 30S ...

journal_title：Nucleic acids research

authors： Prescott CD,Göringer HU

更新日期：1990-09-25 00:00:00

abstract：:Restriction mapping demonstrated the presence of several distinct proviral forms of mouse mammary tumor virus in the genome of GR mice. One of these proviruses (GR-MTV-2) was highly amplified in GR 3A cells, a cell line derived from a GR mammary tumor. By the criterion of restriction mapping, the amplified GR-MTV-2 pr...

journal_title：Nucleic acids research

authors： Fanning TG,Puma JP,Cardiff RD

更新日期：1980-12-11 00:00:00

abstract：:Ionizing radiation induces clustered DNA damage, which presents a challenge to the cellular repair machinery. The repair efficiency of a single-strand break (SSB) is approximately 4x less than that for repair of an abasic (AP) site when in a bistranded cluster containing 8-oxoG. To explore whether this difference in r...

journal_title：Nucleic acids research

authors： Mourgues S,Lomax ME,O'Neill P

更新日期：2007-01-01 00:00:00

abstract：:The repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by the Tp1 family of highly repetitive retrotransposon-like sequences. Tp1 elements consist of two terminal direct repeats of 277bp which flank an internal domain of 8.3kb. They are the major sequence component in th...

journal_title：Nucleic acids research

authors： Rothnie HM,McCurrach KJ,Glover LA,Hardman N

更新日期：1991-01-25 00:00:00

abstract：:One of the obstacles hindering a better understanding of cancer is its heterogeneity. However, computational approaches to model cancer heterogeneity have lagged behind. To bridge this gap, we have developed a new probabilistic approach that models individual cancer cases as mixtures of subtypes. Our approach can be s...

journal_title：Nucleic acids research

authors： Cho DY,Przytycka TM

更新日期：2013-09-01 00:00:00

abstract：:Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected mola...

journal_title：Nucleic acids research

authors： Gegenheimer P

更新日期：1988-03-11 00:00:00

abstract：:At the DNA/RNA level, biological signals are defined by a combination of spatial structures and sequence motifs. Until now, few attempts had been made in writing general purpose search programs that take into account both sequence and structure criteria. Indeed, the most successful structure scanning programs are usua...

journal_title：Nucleic acids research

authors： Billoud B,Kontic M,Viari A

更新日期：1996-04-15 00:00:00

abstract：:A system for the tetracycline-inducible regulation of gene expression in mycobacteria has been developed. We have sub-cloned the tetRO region from the Corynebacterium glutamicum TetZ locus into a mycobacterial shuttle plasmid, making expression of genes cloned downstream of tetRO responsive to tetracycline. Using the ...

journal_title：Nucleic acids research

authors： Blokpoel MC,Murphy HN,O'Toole R,Wiles S,Runn ES,Stewart GR,Young DB,Robertson BD

更新日期：2005-02-01 00:00:00

abstract：:Human U6 small nuclear RNA (U6 snRNA), an abundant snRNA required for splicing of pre-mRNAs, contains several post-transcriptional modifications including a single m6A (N-6-methyladenosine) at position 43. This A-43 residue is critical for the function of U6 snRNA in splicing of pre-mRNAs. Yeast and plant U6 snRNAs al...

journal_title：Nucleic acids research

authors： Shimba S,Bokar JA,Rottman F,Reddy R

更新日期：1995-07-11 00:00:00

abstract：:Successful use and reliability of microarray technology is highly dependent on several factors, including surface chemistry parameters and accessibility of cDNA targets to the DNA probes fixed onto the surface. Here, we show that functionalisation of glass slides with homemade dendrimers allow production of more sensi...

journal_title：Nucleic acids research

authors： Le Berre V,Trévisiol E,Dagkessamanskaia A,Sokol S,Caminade AM,Majoral JP,Meunier B,François J

更新日期：2003-08-15 00:00:00

abstract：:Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous w...

journal_title：Nucleic acids research

authors： Houghton M,Easton MA,Stewart AG,Smith JC,Doel SM,Catlin GH,Lewis HM,Patel TP,Emtage JS,Carey NH,Porter AG

更新日期：1980-07-11 00:00:00