An alternative protein factor which binds the internal promoter of Xenopus 5S ribosomal RNA genes.

abstract：:Preparations of chicken, rat and human nuclear 5S RNA contain two sets of molecules. The set with the lowest electrophoretic mobility (5Sa) contains RNAs identical or closely related to ribosomal 5S RNA from the corresponding animal species. In HeLa cells and rat brain, we only detected an RNA identical to the ribosom...

journal_title：Nucleic acids research

authors： Krol A,Gallinaro H,Lazar E,Jacob M,Branlant C

更新日期：1981-02-25 00:00:00

abstract：:In some preparations of DNA dependent RNA polymerase a new enzymatic activity has been found which catalyzes the condensation of two pyrophosphate molecules, liberated in the process of RNA synthesis, to one molecule of orthophosphate and one molecule of Mg (or Mn) - chelate complex with trimetaphosphate. This activit...

journal_title：Nucleic acids research

authors： Volloch VZ,Rits S,Tumerman L

更新日期：1979-04-01 00:00:00

abstract：:Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fr...

journal_title：Nucleic acids research

authors： Moore R,Spinhirne A,Lai MJ,Preisser S,Li Y,Kang T,Bleris L

更新日期：2015-01-01 00:00:00

abstract：:Differences between species have been suggested to largely reside in the network of connections among the genes. Nevertheless, the rate at which these connections evolve has not been properly quantified. Here, we measure the extent to which co-regulation between pairs of genes is conserved over large phylogenetic dist...

journal_title：Nucleic acids research

authors： Snel B,van Noort V,Huynen MA

更新日期：2004-09-07 00:00:00

abstract：:Computer programs, implemented on an Univac II00/80 computer system, for the identification and characterization of protein coding genes and for the analysis of nucleic acid sequences, are described. ...

journal_title：Nucleic acids research

authors： Pierno G,Barni N,Candurro M,Cipollaro M,Franzè A,Juliano L,Macchiato MF,Mastrocinque G,Moscatelli C,Scarlato V

更新日期：1984-01-11 00:00:00

abstract：:A mass spectral method is described for the detection and identification of unusual nucleotide residues present in DNAs. Analysis by this method of intact, underivatized DNA from salmon sperm, calf thymus, mouse L-cells, wheat germ, M. lysodeikticus, E. Coli, and the bacteriophages 0X-174, fd, and lamda, yields diagno...

journal_title：Nucleic acids research

authors： Wiebers JL

更新日期：1976-11-01 00:00:00

abstract：:A RNA interference (RNAi) like mechanism is involved in elimination of thousands of DNA segments from the developing somatic macronucleus of Tetrahymena, yet how specific internal eliminated sequences (IESs) are recognized remains to be fully elucidated. To define requirements for DNA rearrangement, we performed mutag...

journal_title：Nucleic acids research

authors： Kowalczyk CA,Anderson AM,Arce-Larreta M,Chalker DL

更新日期：2006-01-01 00:00:00

abstract：:Apolipoprotein B (apoB) mRNA editing is a nuclear event that minimally requires the RNA substrate, APOBEC-1 and APOBEC-1 Complementation Factor (ACF). The co-localization of these macro-molecules within the nucleus and the modulation of hepatic apoB mRNA editing activity have been described following a variety of meta...

journal_title：Nucleic acids research

authors： Lehmann DM,Galloway CA,Sowden MP,Smith HC

更新日期：2006-07-04 00:00:00

abstract：:It has recently been demonstrated that nucleobase-density profiles of typical mRNA coding sequences exhibit a complementary relationship with nucleobase-interaction propensity profiles of their cognate protein sequences. This finding supports the idea that the genetic code developed in response to direct binding inter...

journal_title：Nucleic acids research

authors： Hlevnjak M,Zagrovic B

更新日期：2015-03-31 00:00:00

abstract：:Tight cell-cycle regulation of the histone H4-K20 methyltransferase PR-Set7 is essential for the maintenance of genome integrity. In mammals, this mainly involves the interaction of PR-Set7 with the replication factor PCNA, which triggers the degradation of the enzyme by the CRL4CDT2 E3 ubiquitin ligase. PR-Set7 is al...

journal_title：Nucleic acids research

authors： Zouaz A,Fernando C,Perez Y,Sardet C,Julien E,Grimaud C

更新日期：2018-04-06 00:00:00

abstract：:A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of oligonucleotide microarrays by generating readily hybridizable structure-free targets. Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoa...

journal_title：Nucleic acids research

authors： Lahoud G,Timoshchuk V,Lebedev A,Arar K,Hou YM,Gamper H

更新日期：2008-12-01 00:00:00

abstract：:The compilation of 5S rRNA and 5S rRNA gene nucleotide sequences as of 30 September 1996, contains a total of 1661 primary structures of 5S rRNAs or their genes, which is an increase of 928 new sequence entries over the last compilation. It covers sequences from 54 archaea, 449 eubacteria, 34 plastids, nine mitochondr...

journal_title：Nucleic acids research

authors： Specht T,Szymanski M,Barciszewska MZ,Barciszewski J,Erdmann VA

更新日期：1997-01-01 00:00:00

abstract：:In a bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP)/transcription factor binding screen, we identified Mycobacterium smegmatis Ms6479 as the first c-di-GMP-responsive transcriptional factor in mycobacteria. Ms6479 could specifically bind with c-di-GMP and recognize the promoters of 37 lipid transport an...

journal_title：Nucleic acids research

authors： Li W,He ZG

更新日期：2012-12-01 00:00:00

abstract：:Bacterial RNase III plays important roles in the processing and degradation of RNA transcripts. A major goal is to identify the cleavage targets of this endoribonuclease at a transcriptome-wide scale and delineate its in vivo cleavage rules. Here we applied to Escherichia coli grown to either exponential or stationary...

journal_title：Nucleic acids research

authors： Altuvia Y,Bar A,Reiss N,Karavani E,Argaman L,Margalit H

更新日期：2018-11-02 00:00:00

abstract：:The mutations of 76 haemophilia B patients representing the whole population registered with the Malmö haemophilia centre (42) and referrals from the UK, were characterised. RFLP haplotype analysis of the defective genes indicated that 51 single base pair substitutions were definitely of independent origin and 27 of t...

journal_title：Nucleic acids research

authors： Green PM,Montandon AJ,Bentley DR,Ljung R,Nilsson IM,Giannelli F

更新日期：1990-06-11 00:00:00

abstract：:The Escherichia coli AlkB protein (EcAlkB) is a DNA repair enzyme which reverses methylation damage such as 1-methyladenine (1-meA) and 3-methylcytosine (3-meC). The mammalian AlkB homologues ALKBH2 and ALKBH3 display EcAlkB-like repair activity in vitro, but their substrate specificities are different, and ALKBH2 is ...

journal_title：Nucleic acids research

authors： Meza TJ,Moen MN,Vågbø CB,Krokan HE,Klungland A,Grini PE,Falnes PØ

更新日期：2012-08-01 00:00:00

abstract：:Unlike most yeast ribosomal protein genes studied so far the genes coding for S24 and L46 are adjacent on the genome. Sequence analysis showed that the two genes are transcribed divergently, their initiation codons being 630 bp apart. Taking the respective ATG translation start sites as reference points, the 5'- end o...

journal_title：Nucleic acids research

authors： Leer RJ,van Raamsdonk-Duin MM,Kraakman P,Mager WH,Planta RJ

更新日期：1985-02-11 00:00:00

abstract：:A recently reported cDNA clone coding for human promyelocytic L apoferritin, shows some differences with a liver L apoferritin cDNA. We have investigated if these differences are due to the expression of different genes or to an alternative transcription of an unique gene. In this paper we report data suggesting that ...

journal_title：Nucleic acids research

authors： Santoro C,Marone M,Ferrone M,Costanzo F,Colombo M,Minganti C,Cortese R,Silengo L

更新日期：1986-04-11 00:00:00

abstract：:We characterize a component of the E. coli bacterial nucleoid H1a, which accumulates in stationary phase. This protein, identical with the major component of a plasmid-protein complex previously isolated in our laboratory, has a pI close to 7.5. Acrylamide gel electrophoresis and sedimentation in sucrose gradient have...

journal_title：Nucleic acids research

authors： Spassky A,Rimsky S,Garreau H,Buc H

更新日期：1984-07-11 00:00:00

abstract：:A novel approach for the amplification of cDNA ends is described. It requires only minimal amounts of material, a simple cDNA synthesis reaction and a single PCR reaction to amplify the desired 5'- or 3'-ends of a certain cDNA of interest. It combines the so called CapFinder approach with solid phase cDNA synthesis, t...

journal_title：Nucleic acids research

authors： Schramm G,Bruchhaus I,Roeder T

更新日期：2000-11-15 00:00:00

abstract：:A single locus in the mouse, rat and chicken encodes both alkali myosin light chains, MLC1F and MLC3F. This gene has two distinct promoters and gives rise to two different primary transcripts, which are processed by alternative and different modes of splicing to form MLC1F and MLC3F mRNAs. The MLC1F/MLC3F gene is very...

journal_title：Nucleic acids research

authors： Daubas P,Robert B,Garner I,Buckingham M

更新日期：1985-07-11 00:00:00

abstract：:Neuroserpin is an axonally secreted serine protease inhibitor expressed in the nervous system that protects neurons from ischemia-induced apoptosis. Mutant neuroserpin forms have been found polymerized in inclusion bodies in a familial autosomal encephalopathy causing dementia, or associated with epilepsy. Regulation ...

journal_title：Nucleic acids research

authors： Cuadrado A,Navarro-Yubero C,Furneaux H,Kinter J,Sonderegger P,Muñoz A

更新日期：2002-05-15 00:00:00

abstract：:Analysis of the human VK (ref. 2) gene locus led to the detection of a new sequence family (L sequences). Its copy number is in the range of 10(2). The L sequences, which are about 500 bp long, are found as part of the 3' flanking regions of a clustered set of human VKI genes but they occur also separate from the gene...

journal_title：Nucleic acids research

authors： Straubinger B,Pech M,Mühlebach K,Jaenichen HR,Bauer HG,Zachau HG

更新日期：1984-07-11 00:00:00

abstract：:Genomic DNA methylation profiles exhibit substantial variation within the human population, with important functional implications for gene regulation. So far little is known about the characteristics and determinants of DNA methylation variation among healthy individuals. We performed bioinformatic analysis of high-r...

journal_title：Nucleic acids research

authors： Bock C,Walter J,Paulsen M,Lengauer T

更新日期：2008-06-01 00:00:00

abstract：:The nature of mammalian origins of DNA replication remains controversial and this is primarily because two-dimensional gel replicon mapping techniques have identified broad zones of replication initiation whereas several other techniques, such as quantitative PCR, have disclosed more discrete sites of initiation at th...

journal_title：Nucleic acids research

authors： Toledo F,Baron B,Fernandez MA,Lachagès AM,Mayau V,Buttin G,Debatisse M

更新日期：1998-05-15 00:00:00

abstract：:The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant wi...

journal_title：Nucleic acids research

authors： Sørensen PD,Frederiksen S

更新日期：1991-08-11 00:00:00

abstract：:The last 82 nucleotides of the 6.3 kb genomic RNA of plant turnip yellow mosaic virus (TYMV), the so-called 'tRNA-like' domain, presents functional, structural and primary sequence homologies with canonical tRNAs. In particular, one of the stem-loops resembles the TPsi(pseudouridine)-branch of tRNA, except for the pre...

journal_title：Nucleic acids research

authors： Becker HF,Motorin Y,Florentz C,Giegé R,Grosjean H

更新日期：1998-09-01 00:00:00

abstract：:Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crys...

journal_title：Nucleic acids research

authors： Olal D,Dick A,Woods VL Jr,Liu T,Li S,Devignot S,Weber F,Saphire EO,Daumke O

更新日期：2014-05-01 00:00:00

abstract：:A suppressor tRNA(Tyr) and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-L-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNA(Tyr) gene was mutated, at three positions in the D arm, to generate the internal promoter for expression. However, this tRN...

journal_title：Nucleic acids research

authors： Sakamoto K,Hayashi A,Sakamoto A,Kiga D,Nakayama H,Soma A,Kobayashi T,Kitabatake M,Takio K,Saito K,Shirouzu M,Hirao I,Yokoyama S

更新日期：2002-11-01 00:00:00

abstract：:The Xist lncRNA requires Repeat A, a conserved RNA element located in its 5' end, to induce gene silencing during X-chromosome inactivation. Intriguingly, Repeat A is also required for production of Xist. While silencing by Repeat A requires the protein SPEN, how Repeat A promotes Xist production remains unclear. We r...

journal_title：Nucleic acids research

authors： Trotman JB,Lee DM,Cherney RE,Kim SO,Inoue K,Schertzer MD,Bischoff SR,Cowley DO,Calabrese JM

更新日期：2020-10-09 00:00:00