Structural and functional adaptation of Haloferax volcanii TFEα/β.

abstract：:RNA thermometers control the translation of several heat shock and virulence genes by their temperature-sensitive structural transitions. Changes in the structure and dynamics of MiniROSE RNA, which regulates translation in the temperature range of 20-45°C, were studied by site specifically replacing seven adenine res...

journal_title：Nucleic acids research

authors： Narayan S,Kombrabail MH,Das S,Singh H,Chary KV,Rao BJ,Krishnamoorthy G

更新日期：2015-01-01 00:00:00

abstract：:The efficiency of Nucleotide Excision Repair (NER)process is crucial for maintaining genomic integrity because in many organisms, including humans, it represents the only system able to repair a wide range of DNA damage. The aim of the work was to investigate whether the efficiency of the repair of photoproducts induc...

journal_title：Nucleic acids research

authors： Bee L,Marini S,Pontarin G,Ferraro P,Costa R,Albrecht U,Celotti L

更新日期：2015-02-27 00:00:00

abstract：:Influenza A virus segment 2 mRNA expresses three polypeptides: PB1, PB1-F2 and PB1-N40, from AUGs 1, 4 and 5 respectively. Two short open reading frames (sORFs) initiated by AUGs 2 and 3 are also present. To understand translational regulation in this system, we systematically mutated AUGs 1-4 and monitored polypeptid...

journal_title：Nucleic acids research

authors： Wise HM,Barbezange C,Jagger BW,Dalton RM,Gog JR,Curran MD,Taubenberger JK,Anderson EC,Digard P

更新日期：2011-09-01 00:00:00

abstract：:DNA triplet repeats, 5'-d(CTG)n and 5'-d(CAG)n, are present in genes which have been implicated in several neurodegenerative disorders. To investigate possible stable structures formed by these repeating sequences, we have examined d(CTG)n, d(CAG)n and d(CTG).d(CAG)n (n = 2 and 3) using NMR and UV optical spectroscopy...

journal_title：Nucleic acids research

authors： Smith GK,Jie J,Fox GE,Gao X

更新日期：1995-11-11 00:00:00

abstract：:Most mutations in cancer genomes occur in the non-coding regions with unknown impact on tumor development. Although the increase in the number of cancer whole-genome sequences has revealed numerous putative non-coding cancer drivers, their information is dispersed across multiple studies making it difficult to underst...

journal_title：Nucleic acids research

authors： Liu EM,Martinez-Fundichely A,Bollapragada R,Spiewack M,Khurana E

更新日期：2021-01-08 00:00:00

abstract：:Interactive Tree Of Life (http://itol.embl.de) is a web-based tool for the display, manipulation and annotation of phylogenetic trees. It is freely available and open to everyone. In addition to classical tree viewer functions, iTOL offers many novel ways of annotating trees with various additional data. Current versi...

journal_title：Nucleic acids research

authors： Letunic I,Bork P

更新日期：2011-07-01 00:00:00

abstract：:The dominant kanr marker gene plays an important role in gene disruption experiments in budding yeast, as this marker can be used in a variety of yeast strains lacking the conventional yeast markers. We have developed a loxP-kanMX-loxP gene disruption cassette, which combines the advantages of the heterologous kanr ma...

journal_title：Nucleic acids research

authors： Güldener U,Heck S,Fielder T,Beinhauer J,Hegemann JH

更新日期：1996-07-01 00:00:00

abstract：:Immunoglobulin (Ig) diversification by somatic hypermutation in germinal center B cells is instrumental for maturation of the humoral immune response, but also bears the risk of excessive or aberrant genetic changes. Thus, introduction of DNA damage by activation-induced cytidine deaminase as well as DNA repair by mul...

journal_title：Nucleic acids research

authors： Frankenberger S,Davari K,Fischer-Burkart S,Böttcher K,Tomi NS,Zimber-Strobl U,Jungnickel B

更新日期：2014-04-01 00:00:00

abstract：:Riboswitches are important gene regulatory elements frequently encountered in bacterial mRNAs. The recently discovered nadA riboswitch contains two similar, tandemly arrayed aptamer domains, with the first domain possessing high affinity for nicotinamide adenine dinucleotide (NAD+). The second domain which comprises t...

journal_title：Nucleic acids research

authors： Chen H,Egger M,Xu X,Flemmich L,Krasheninina O,Sun A,Micura R,Ren A

更新日期：2020-12-02 00:00:00

abstract：:Highly conserved sequences present at an identical position near the 3' ends of eukaryotic and prokaryotic 5S rRNAs are complementary to the 5' strand of the m2(6)A hairpin structure near the 3' ends of 18S rRNA and 16S rRNA, respectively. The extent of base-pairing and the calculated stabilities of the hybrids that c...

journal_title：Nucleic acids research

authors： Azad AA

更新日期：1979-12-11 00:00:00

abstract：:Transcription factors (TFs) regulate gene expression by binding to short DNA sequence motifs, yet their binding specificities alone cannot explain how certain TFs drive a diversity of biological processes. In order to investigate the factors that control the functions of the pleiotropic TF STAT3, we studied its genome...

journal_title：Nucleic acids research

authors： Hutchins AP,Diez D,Takahashi Y,Ahmad S,Jauch R,Tremblay ML,Miranda-Saavedra D

更新日期：2013-02-01 00:00:00

abstract：:Non-homologous end-joining (NHEJ) of DNA double-strand breaks (DSBs) is mediated by two protein complexes comprising Ku80/Ku70/DNA-PKcs/Artemis and XRCC4/LigaseIV/XLF. Loss of Ku or XRCC4/LigaseIV function compromises the rejoining of radiation-induced DSBs and leads to defective V(D)J recombination. In this study, we...

journal_title：Nucleic acids research

authors： Schulte-Uentrop L,El-Awady RA,Schliecker L,Willers H,Dahm-Daphi J

更新日期：2008-05-01 00:00:00

abstract：:Transcription of 5S RNA gene in Xenopus oocytes requires a 38 kDa transcription factor TFIIIA, which interacts with the 50 bp internal control region of the gene. We looked for TFIIIA-like polypeptides in the extracts of adult Xenopus tissues on the basis of their antigenic cross-reactivity to anti-TFIIIA antibody. Se...

journal_title：Nucleic acids research

authors： Yasui W,Ryoji M

更新日期：1989-07-25 00:00:00

abstract：:An unexpectedly high number of regulatory RNAs have been recently discovered that fine-tune the function of genes at all levels of expression. We employed Genomic SELEX, a method to identify protein-binding RNAs encoded in the genome, to search for further regulatory RNAs in Escherichia coli. We used the global regula...

journal_title：Nucleic acids research

authors： Lorenz C,Gesell T,Zimmermann B,Schoeberl U,Bilusic I,Rajkowitsch L,Waldsich C,von Haeseler A,Schroeder R

更新日期：2010-06-01 00:00:00

abstract：:In this study, we show that silencing of CITED2 using small-hairpin RNA (shCITED2) induced DNA damage and reduction of ERCC1 gene expression in HEK293, HeLa and H1299 cells, even in the absence of cisplatin. In contrast, ectopic expression of ERCC1 significantly reduced intrinsic and induced DNA damage levels, and res...

journal_title：Nucleic acids research

authors： Liu YC,Chang PY,Chao CC

更新日期：2015-12-15 00:00:00

abstract：:The nucleotide sequences 3' to the translational termination codons in a collection of human genes have been analysed for evidence of a preferred 3' context for natural UAG codons. The aim was to see whether human UAG contexts can be related to the recent demonstration of the effects of 3' context on nonsense suppress...

journal_title：Nucleic acids research

authors： Martin R

更新日期：1994-01-11 00:00:00

abstract：:In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...

journal_title：Nucleic acids research

authors： Stefanovic B,Hackl W,Lührmann R,Schümperli D

更新日期：1995-08-25 00:00:00

abstract：:A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amin...

journal_title：Nucleic acids research

authors： Graham RW,Atkinson T,Kilburn DG,Miller RC Jr,Warren RA

更新日期：1993-10-25 00:00:00

abstract：:The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 am...

journal_title：Nucleic acids research

authors： Stefan C,Xia YN,Van Etten JL

更新日期：1991-01-25 00:00:00

abstract：:The Genome Database for Rosaceae (GDR, http:/www.rosaceae.org), the long-standing central repository and data mining resource for Rosaceae research, has been enhanced with new genomic, genetic and breeding data, and improved functionality. Whole genome sequences of apple, peach and strawberry are available to browse o...

journal_title：Nucleic acids research

authors： Jung S,Ficklin SP,Lee T,Cheng CH,Blenda A,Zheng P,Yu J,Bombarely A,Cho I,Ru S,Evans K,Peace C,Abbott AG,Mueller LA,Olmstead MA,Main D

更新日期：2014-01-01 00:00:00

abstract：:Numerous examples exist of how disrupting the actions of physiological regulators of blood cell development yields hematologic malignancies. The master regulator of hematopoietic stem/progenitor cells GATA-2 was cloned almost 20 years ago, and elegant genetic analyses demonstrated its essential function to promote hem...

journal_title：Nucleic acids research

authors： Bresnick EH,Katsumura KR,Lee HY,Johnson KD,Perkins AS

更新日期：2012-07-01 00:00:00

abstract：:DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less...

journal_title：Nucleic acids research

authors： Yoshida S,Ungers G,Rosenberg BH

更新日期：1977-01-01 00:00:00

abstract：:Calf lens messenger fractions have been purified on poly(dT)-cellulose. The purified RNA's have been analyzed as nucleosides by a method which can be used on a micro-scale with highly reproduceble results. Both the 10S and 14S lens mRNA contain poly A sequences. The poly A content found in 14S mRNA cannot account for ...

journal_title：Nucleic acids research

authors： Piperno G,Bertazzoni U,Berns AJ,Bloemendal H

更新日期：1974-02-01 00:00:00

abstract：:Alternative splicing (AS) and alternative polyadenylation (APA) generate diverse transcripts in mammalian genomes during development and differentiation. Epigenetic marks such as trimethylation of histone H3 lysine 36 (H3K36me3) and DNA methylation play a role in generating transcriptome diversity. Intragenic CpG isla...

journal_title：Nucleic acids research

authors： Amante SM,Montibus B,Cowley M,Barkas N,Setiadi J,Saadeh H,Giemza J,Contreras-Castillo S,Fleischanderl K,Schulz R,Oakey RJ

更新日期：2020-09-04 00:00:00

abstract：:The PR264/SC35 splicing factor belongs to the family of SR proteins which function as essential and alternative splicing factors. Here, we report that the human PR264/SC35 locus is bidirectionally transcribed. Double in situ hybridization experiments have allowed simultaneous detection of sense and antisense RNA in hu...

journal_title：Nucleic acids research

authors： Sureau A,Soret J,Guyon C,Gaillard C,Dumon S,Keller M,Crisanti P,Perbal B

更新日期：1997-11-15 00:00:00

abstract：:A requirement for specific RNA folding is that the free-energy landscape discriminate against non-native folds. While tertiary interactions are critical for stabilizing the native fold, they are relatively non-specific, suggesting additional mechanisms contribute to tertiary folding specificity. In this study, we use ...

journal_title：Nucleic acids research

authors： Mustoe AM,Al-Hashimi HM,Brooks CL 3rd

更新日期：2016-01-08 00:00:00

abstract：:In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneou...

journal_title：Nucleic acids research

authors： Gambaryan AS,Venkstern TV,Bayev AA

更新日期：1976-08-01 00:00:00

abstract：:Anti-microRNA (miRNA) oligonucleotides (AMOs) with 2'-O-Methyl (2'OMe) residues are commonly used to study miRNA function and can achieve high potency, with low cytotoxicity. Not withstanding this, we demonstrate the sequence-dependent capacity of 2'OMe AMOs to inhibit Toll-like receptor (TLR) 7 and 8 sensing of immun...

journal_title：Nucleic acids research

authors： Sarvestani ST,Stunden HJ,Behlke MA,Forster SC,McCoy CE,Tate MD,Ferrand J,Lennox KA,Latz E,Williams BR,Gantier MP

更新日期：2015-01-01 00:00:00

abstract：:Few transcriptional regulatory networks have been described in non-model organisms. In Entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. We used expression data from tw...

journal_title：Nucleic acids research

authors： Hackney JA,Ehrenkaufer GM,Singh U

更新日期：2007-01-01 00:00:00

abstract：:The Evidence and Conclusion Ontology (ECO) contains terms (classes) that describe types of evidence and assertion methods. ECO terms are used in the process of biocuration to capture the evidence that supports biological assertions (e.g. gene product X has function Y as supported by evidence Z). Capture of this inform...

journal_title：Nucleic acids research

authors： Giglio M,Tauber R,Nadendla S,Munro J,Olley D,Ball S,Mitraka E,Schriml LM,Gaudet P,Hobbs ET,Erill I,Siegele DA,Hu JC,Mungall C,Chibucos MC

更新日期：2019-01-08 00:00:00