Abstract:
:The dominant kanr marker gene plays an important role in gene disruption experiments in budding yeast, as this marker can be used in a variety of yeast strains lacking the conventional yeast markers. We have developed a loxP-kanMX-loxP gene disruption cassette, which combines the advantages of the heterologous kanr marker with those from the Cre-lox P recombination system. This disruption cassette integrates with high efficiency via homologous integration at the correct genomic locus (routinely 70%). Upon expression of the Cre recombinase the kanMX module is excised by an efficient recombination between the loxP sites, leaving behind a single loxP site at the chromosomal locus. This system allows repeated use of the kanr marker gene and will be of great advantage for the functional analysis of gene families.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Güldener U,Heck S,Fielder T,Beinhauer J,Hegemann JHdoi
10.1093/nar/24.13.2519subject
Has Abstractpub_date
1996-07-01 00:00:00pages
2519-24issue
13eissn
0305-1048issn
1362-4962pii
6w0051journal_volume
24pub_type
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