Site-specific fluorescence dynamics in an RNA 'thermometer' reveals the role of ribosome binding in its temperature-sensitive switch function.

abstract：:A sequence of 89 nucleotides from rabbit beta-globin mRNA has been determined and is shown to code for residues 107 to 137 of the beta-globin protein. In addition, a sequence heterogeneity has been identified within this 89 nucleotide long sequence which corresponds to a known polymorphic variant of rabbit beta-globin...

journal_title：Nucleic acids research

authors： Proudfoot NJ

更新日期：1976-07-01 00:00:00

abstract：:The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely rela...

journal_title：Nucleic acids research

authors： Zeng Y,Wang Y

更新日期：2006-01-01 00:00:00

abstract：:Bulky lesions in the template strand block the progression of RNA polymerase II (RNAP II) and are repaired more rapidly than lesions in the non-transcribed strand, which do not block transcription. In order to better understand the basis of this transcription-coupled repair we developed an in vitro system with purifie...

journal_title：Nucleic acids research

authors： Selby CP,Drapkin R,Reinberg D,Sancar A

更新日期：1997-02-15 00:00:00

abstract：:The synthesis of a pentadecamer of the 5'-phosphate of the antiviral nucleoside ribavirin (1'-beta-D-ribofuranosyl-1H-1,2,4-triazole-3-carboxamide) has been achieved. This homoribopolymer is terminated at the 5'-position with an (6-aminohexyl)phosphate group to permit conjugation to a carrier and at the 3'-position by...

journal_title：Nucleic acids research

authors： Dawson MI,Jina AN,Torkelson S,Rhee S,Moore M,Zarling DA,Hobbs PD

更新日期：1990-03-11 00:00:00

abstract：:FANTOM DB, the database of Functional Annotation of RIKEN Mouse cDNA Clones, is designed to store sequence information of RIKEN full-length enriched mouse cDNA clones, graphical views of sequence analysis results, curated functional annotation information and additional descriptions, including Gene Ontology terms. RIK...

journal_title：Nucleic acids research

authors： Bono H,Kasukawa T,Furuno M,Hayashizaki Y,Okazaki Y

更新日期：2002-01-01 00:00:00

abstract：:MicroArray Gene expression and Network Evaluation Toolkit (MAGNET) is a web-based application that provides tools to generate and score both protein-protein interaction networks and coexpression networks. MAGNET integrates user-provided experimental measurements with high-throughput proteomic datasets, generating weig...

journal_title：Nucleic acids research

authors： Linderman GC,Chance MR,Bebek G

更新日期：2012-07-01 00:00:00

abstract：:ATRX and MeCP2 belong to an expanding group of chromatin-associated proteins implicated in human neurodevelopmental disorders, although their gene-regulatory activities are not fully resolved. Loss of ATRX prevents full repression of an imprinted gene network in the postnatal brain and in this study we address the mec...

journal_title：Nucleic acids research

authors： Kernohan KD,Vernimmen D,Gloor GB,Bérubé NG

更新日期：2014-07-01 00:00:00

abstract：:A novel program suite was implemented for the functional interpretation of high-throughput gene expression data based on the identification of Gene Ontology (GO) nodes. The focus of the analysis lies on the interpretation of microarray data from prokaryotes. The three well established statistical methods of the thresh...

journal_title：Nucleic acids research

authors： Scheer M,Klawonn F,Münch R,Grote A,Hiller K,Choi C,Koch I,Schobert M,Härtig E,Klages U,Jahn D

更新日期：2006-07-01 00:00:00

abstract：:High throughput sequencing (HTS) platforms generate unprecedented amounts of data that introduce challenges for processing and downstream analysis. While tools that report the 'best' mapping location of each read provide a fast way to process HTS data, they are not suitable for many types of downstream analysis such a...

journal_title：Nucleic acids research

authors： Hach F,Sarrafi I,Hormozdiari F,Alkan C,Eichler EE,Sahinalp SC

更新日期：2014-07-01 00:00:00

abstract：:A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling o...

journal_title：Nucleic acids research

authors： Wesley CS,Ben M,Kreitman M,Hagag N,Eanes WF

更新日期：1990-02-11 00:00:00

abstract：:This laboratory has established in previous studies that Pmel 17, a gene expressed specifically in melanocytes, maps near the silver coat color locus (si/si) on mouse chromosome 10. In the current study, we have focused on determining whether or not the si allele carries a mutation in Pmel 17. Pmel 17 cDNA clones, iso...

journal_title：Nucleic acids research

authors： Kwon BS,Halaban R,Ponnazhagan S,Kim K,Chintamaneni C,Bennett D,Pickard RT

更新日期：1995-01-11 00:00:00

abstract：:In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease-exonuclease-phosphatase domain....

journal_title：Nucleic acids research

authors： Maryati M,Kaur I,Jadhav GP,Olotu-Umoren L,Oveh B,Hashmi L,Fischer PM,Winkler GS

更新日期：2014-03-01 00:00:00

abstract：:G-quadruplexes (G4s) are non-canonical nucleic acids secondary structures that form within guanine-rich strands of regulatory genomic regions. G4s have been extensively described in the human genome, especially in telomeres and oncogene promoters; in recent years the presence of G4s in viruses has attracted increasing...

journal_title：Nucleic acids research

authors： Ruggiero E,Richter SN

更新日期：2018-04-20 00:00:00

abstract：:Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. He...

journal_title：Nucleic acids research

authors： Oomoto I,Suzuki-Hirano A,Umeshima H,Han YW,Yanagisawa H,Carlton P,Harada Y,Kengaku M,Okamoto A,Shimogori T,Wang DO

更新日期：2015-10-30 00:00:00

abstract：:Plasmids p749, p106, and p150 contain cDNA inserts complementary to rat skeletal muscle actin mRNA. Nucleotide sequence analysis indicates the following sequence relationships: p749 specifies codons 171 to 360; p150 specifies codons 357 to 374 together with 120 nucleotides of the 3'-non-translated region; p106 specifi...

journal_title：Nucleic acids research

authors： Shani M,Nudel U,Zevin-Sonkin D,Zakut R,Givol D,Katcoff D,Carmon Y,Reiter J,Frischauf AM,Yaffe D

更新日期：1981-02-11 00:00:00

abstract：:Gene rewiring is a common evolutionary phenomenon in nature that may lead to extinction for living organisms. Recent studies on synthetic biology demonstrate that cells can survive genetic rewiring. This survival (adaptation) is often linked to the stochastic expression of rewired genes with random transcriptional cha...

journal_title：Nucleic acids research

authors： Murakami Y,Matsumoto Y,Tsuru S,Ying BW,Yomo T

更新日期：2015-01-01 00:00:00

abstract：:A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has ...

journal_title：Nucleic acids research

authors： Gingeras TR,MIlazzo JP,Roberts RJ

更新日期：1978-11-01 00:00:00

abstract：:Antisense oligodeoxynucleotides targeted to Ha-ras mRNA have been designed to discriminate between the codon 12-mutated oncogene and the normal proto-oncogene. An in vitro assay using two different sources of RNase H (rabbit reticulocyte lysates and nuclear extract from HeLa cells) was used to characterize oligonucleo...

journal_title：Nucleic acids research

authors： Duroux I,Godard G,Boidot-Forget M,Schwab G,Hélène C,Saison-Behmoaras T

更新日期：1995-09-11 00:00:00

abstract：:The Bcl-2 protein has an anti-apoptotic effect in neuronal and other cell types. We show for the first time that the Bcl-2 promoter is activated by the neuronal survival factor nerve growth factor (NGF) and that this effect is dependent on a region of the promoter from -1472 to -1414. This activation requires the Rap-...

journal_title：Nucleic acids research

authors： Liu YZ,Boxer LM,Latchman DS

更新日期：1999-05-15 00:00:00

abstract：:Approaches to characterize the nucleic acid-binding properties of drugs and druglike small molecules are crucial to understanding the behavior of these compounds in cellular systems. Here, we use a Small Molecule Microarray (SMM) profiling approach to identify the preferential interaction between chlorhexidine, a wide...

journal_title：Nucleic acids research

authors： Calabrese DR,Zlotkowski K,Alden S,Hewitt WM,Connelly CM,Wilson RM,Gaikwad S,Chen L,Guha R,Thomas CJ,Mock BA,Schneekloth JS Jr

更新日期：2018-04-06 00:00:00

abstract：:The DNA data bank of Japan (DDBJ, http://www.ddbj.nig.ac.jp) maintains a primary nucleotide sequence database and provides analytical resources for biological information to researchers. This database content is exchanged with the US National Center for Biotechnology Information (NCBI) and the European Bioinformatics ...

journal_title：Nucleic acids research

authors： Ogasawara O,Mashima J,Kodama Y,Kaminuma E,Nakamura Y,Okubo K,Takagi T

更新日期：2013-01-01 00:00:00

abstract：:Silencing gene expression by siRNAs is rapidly becoming a powerful tool for the genetic analysis of mammalian cells. However, the rapid degradation of siRNA and the limited duration of its action call for an efficient delivery technology. Accordingly, we describe here that Atelocollagen complexed with siRNA is resista...

journal_title：Nucleic acids research

authors： Minakuchi Y,Takeshita F,Kosaka N,Sasaki H,Yamamoto Y,Kouno M,Honma K,Nagahara S,Hanai K,Sano A,Kato T,Terada M,Ochiya T

更新日期：2004-07-22 00:00:00

abstract：:We characterized and purified a protein from rat liver which specifically binds to a DNA motif present in a liver-specific enhancer of the rat tyrosine aminotransferase (TAT) gene, when offered as single-stranded DNA. Binding is highly sequence-specific and coincides with a region known to be essential for function of...

journal_title：Nucleic acids research

authors： Jansen-Dürr P,Boshart M,Lupp B,Bosserhoff A,Frank RW,Schütz G

更新日期：1992-03-25 00:00:00

abstract：:Helicase loading at a DNA replication origin often requires the dynamic interactions between the DNA helicase and an accessory protein. In E. coli, the DNA helicase is DnaB and DnaC is its loading partner. We used the method of hydrogen/deuterium exchange mass spectrometry to address the importance of DnaB-DnaC comple...

journal_title：Nucleic acids research

authors： Chodavarapu S,Jones AD,Feig M,Kaguni JM

更新日期：2016-01-08 00:00:00

abstract：:Oligo(dG).oligo(dC)- or short poly(dG).poly(dC)-containing fragments were enriched and cloned by means of Mg2+-dependent triplex affinity capture and subsequent cloning procedures. A library constructed after three cycles of enrichment showed that approximately 80% of the clones in the supercoiled form formed a comple...

journal_title：Nucleic acids research

authors： Nishikawa N,Kanda N,Oishi M,Kiyama R

更新日期：1997-05-01 00:00:00

abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...

journal_title：Nucleic acids research

authors： Seurinck J,Van de Voorde A,Van Montagu M

更新日期：1983-07-11 00:00:00

abstract：:ExPASy (http://www.expasy.org) has worldwide reputation as one of the main bioinformatics resources for proteomics. It has now evolved, becoming an extensible and integrative portal accessing many scientific resources, databases and software tools in different areas of life sciences. Scientists can henceforth access s...

journal_title：Nucleic acids research

authors： Artimo P,Jonnalagedda M,Arnold K,Baratin D,Csardi G,de Castro E,Duvaud S,Flegel V,Fortier A,Gasteiger E,Grosdidier A,Hernandez C,Ioannidis V,Kuznetsov D,Liechti R,Moretti S,Mostaguir K,Redaschi N,Rossier G,Xenarios

更新日期：2012-07-01 00:00:00

abstract：:Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of m...

journal_title：Nucleic acids research

authors： Lemaigre FP,Ace CI,Green MR

更新日期：1993-06-25 00:00:00

abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...

journal_title：Nucleic acids research

authors： Turro E,Lewin A,Rose A,Dallman MJ,Richardson S

更新日期：2010-01-01 00:00:00

abstract：:50 to 55% of tobacco and barley nuclear DNA is accessible to micrococcal endonuclease digestion. The DNA fragments resulting from a mild endonuclease treatment are multiples of a basic unit of 194 +/- 6 base pairs in tobacco and 195 +/- 6 base pairs in barley. After extensive digestion, a DNA fragment of approximately...

journal_title：Nucleic acids research

authors： Philipps G,Gigot C

更新日期：1977-10-01 00:00:00