Abstract:
:A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of 0X174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Gingeras TR,MIlazzo JP,Roberts RJdoi
10.1093/nar/5.11.4105subject
Has Abstractpub_date
1978-11-01 00:00:00pages
4105-27issue
11eissn
0305-1048issn
1362-4962journal_volume
5pub_type
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