Abstract:
:A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several sup4 genes with 5'- and 3'-terminal deletions in the homologous transcription system. Thirty-four different sup4 genes with spontaneous mutations were also tested in the in vitro system. Two point mutations drastically reduced transcription initiation and two other mutations caused premature termination. These mutations have nearly identical effects on SUP4 gene transcription by Xenopus RNA polymerase III (1), which demonstrates that the essential features of RNA polymerase III transcription initiation and termination signals have been conserved throughout the course of eukaryotic evolution.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Koski RA,Allison DS,Worthington M,Hall BDdoi
10.1093/nar/10.24.8127subject
Has Abstractpub_date
1982-12-20 00:00:00pages
8127-43issue
24eissn
0305-1048issn
1362-4962journal_volume
10pub_type
杂志文章abstract::DNA methylation plays an important role in many biological processes. Existing epigenome-wide association studies (EWAS) have successfully identified aberrantly methylated genes in many diseases and disorders with most studies focusing on analysing methylation sites one at a time. Incorporating prior biological inform...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw546
更新日期:2016-09-19 00:00:00
abstract::The EcoCyc database (http://EcoCyc.org/) is a comprehensive source of information on the biology of the prototypical model organism Escherichia coli K12. The mission for EcoCyc is to contain both computable descriptions of, and detailed comments describing, all genes, proteins, pathways and molecular interactions in E...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.18.4537
更新日期:1981-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3873
更新日期:1996-10-01 00:00:00
abstract::The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible m...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1980-04-25 00:00:00
abstract::To locate the boundaries of the TATA element in the SV40 early promoter, point mutations have been constructed such as to cover the whole T + A-rich region of the replication origin. The effects of these mutations on the rate of transcription in vivo show that this region actually contains two TATA elements I and II, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.5.975
更新日期:1992-03-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky024
更新日期:2018-02-28 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn218
更新日期:2008-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.23.6247
更新日期:1992-12-11 00:00:00
abstract::Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.22.6209
更新日期:1991-11-25 00:00:00
abstract::Phage_Finder, a heuristic computer program, was created to identify prophage regions in completed bacterial genomes. Using a test dataset of 42 bacterial genomes whose prophages have been manually identified, Phage_Finder found 91% of the regions, resulting in 7% false positive and 9% false negative prophages. A searc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl732
更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1223
更新日期:2016-03-18 00:00:00
abstract::The DNA repair enzyme human uracil DNA glycosylase (UNG) scans short stretches of genomic DNA and captures rare uracil bases as they transiently emerge from the DNA duplex via spontaneous base pair breathing motions. The process of DNA scanning requires that the enzyme transiently loosen its grip on DNA to allow stoch...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp161
更新日期:2009-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
abstract::Next-generation sequencing is excellently suited to evaluate the abundance of mRNAs to study gene expression. Here we compare two alternative technologies, cap analysis of gene expression (CAGE) and serial analysis of gene expression (SAGE), for the same RNA samples. Along with quantifying gene expression levels, CAGE...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq602
更新日期:2010-09-01 00:00:00
abstract::RBD-1 is the Caenorhabditis elegans homolog of Mrd1p, which was recently shown to be required for 18S ribosomal RNA (rRNA) processing in yeast. To gain insights into the relationship between ribosome biogenesis and the development of multicellular organisms, we examined the expression and function of RBD-1. Maternal R...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh264
更新日期:2004-02-10 00:00:00
abstract::Nam8, a component of yeast U1 snRNP, is optional for mitotic growth but required during meiosis, because Nam8 collaborates with Mer1 to promote splicing of essential meiotic mRNAs AMA1, MER2 and MER3. Here, we identify SPO22 and PCH2 as novel targets of Nam8-dependent meiotic splicing. Whereas SPO22 splicing is co-dep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1328
更新日期:2011-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1990-05-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg919
更新日期:2004-01-02 00:00:00
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journal_title:Nucleic acids research
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更新日期:2010-05-01 00:00:00
abstract::Benzo[c]phenanthrene dihydrodiol epoxide (B[c] PhDE) is well known as an important environmental chemical carcinogen that preferentially modifies DNA in adenine residues. However, the molecular mechanism by which B[c]PhDE induces tumorigenesis is not fully understood. In this report, we demonstrate that DNA mismatch r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg875
更新日期:2003-11-15 00:00:00
abstract::Recent advances in next-generation sequencing technologies and genome assembly algorithms have enabled the accumulation of a huge volume of genome sequences from various species. This has provided new opportunities for large-scale comparative genomics studies. Identifying and utilizing synteny blocks, which are genomi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw310
更新日期:2016-07-08 00:00:00
abstract::p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1075
更新日期:2007-01-01 00:00:00
abstract::Eukaryotic tRNAs are transcribed as precursors. A 5'-end leader and 3'-end trailer are endonucleolytically removed by RNase P and 3'-tRNase before 3'-end CCA addition, aminoacylation, nuclear export and translation. 3'-End -CC can be a 3'-tRNase anti-determinant with the ability to prevent mature tRNA from recycling t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.21.4334
更新日期:2001-11-01 00:00:00
abstract::Directed molecular evolution and combinatorial methodologies are playing an increasingly important role in the field of protein engineering. The general approach of generating a library of partially randomized genes, expressing the gene library to generate the proteins the library encodes and then screening the protei...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2004-02-27 00:00:00
abstract::Recent studies with poly(ADP-ribose) polymerase (PARP)-deficient mice have highlighted the role of this enzyme in genomic stability and response to various genomic insults. In the absence of DNA damaging treatment, we report here that a PARP-deficient cell line (PARP-/-) established from knockout mice displays a decre...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.22.5134
更新日期:1998-11-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm521
更新日期:2007-01-01 00:00:00
abstract::Splicing is a biological phenomenon that removes the non-coding sequence from the transcripts to produce a mature transcript suitable for translation. To study this phenomenon, information on the intron-exon arrangement of a gene is essential, usually obtained by aligning mRNA/EST sequences to their cognate genomic se...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg561
更新日期:2003-07-01 00:00:00