Determinants of Nam8-dependent splicing of meiotic pre-mRNAs.

abstract：:Tetramers of the arginine-rich histones H3 and H4 associate with supercoiled SV40 DNA either singly, giving tetrameric nucleoprotein complexes or in pairs giving octameric complexes, both of which are visualized as beads in the electron microscope. The relative amounts of the two complexes may be revealed by complete ...

journal_title：Nucleic acids research

authors： Thomas JO,Oudet P

更新日期：1979-10-10 00:00:00

abstract：:In this study, we show that silencing of CITED2 using small-hairpin RNA (shCITED2) induced DNA damage and reduction of ERCC1 gene expression in HEK293, HeLa and H1299 cells, even in the absence of cisplatin. In contrast, ectopic expression of ERCC1 significantly reduced intrinsic and induced DNA damage levels, and res...

journal_title：Nucleic acids research

authors： Liu YC,Chang PY,Chao CC

更新日期：2015-12-15 00:00:00

abstract：:In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how the different KH modules participate in the recognition of the RNA targets is necessary to dissect the way these protein...

journal_title：Nucleic acids research

authors： Hollingworth D,Candel AM,Nicastro G,Martin SR,Briata P,Gherzi R,Ramos A

更新日期：2012-08-01 00:00:00

abstract：:RNA duplex stability depends strongly on ionic conditions, and inside cells RNAs are exposed to both monovalent and multivalent ions. Despite recent advances, we do not have general methods to quantitatively account for the effects of monovalent and multivalent ions on RNA stability, and the thermodynamic parameters f...

journal_title：Nucleic acids research

authors： Bizarro CV,Alemany A,Ritort F

更新日期：2012-08-01 00:00:00

abstract：:Mammalian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation. Phosphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication. Here, we show 14-3-3 is a reader protein of DNMT1pSer143. In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA replication. The ...

journal_title：Nucleic acids research

authors： Estève PO,Zhang G,Ponnaluri VK,Deepti K,Chin HG,Dai N,Sagum C,Black K,Corrêa IR Jr,Bedford MT,Cheng X,Pradhan S

更新日期：2016-02-29 00:00:00

abstract：:Expansions of (GAA)n repeats within the first intron of the frataxin gene reduce its expression, resulting in a hereditary neurodegenerative disorder, Friedreich's ataxia. While it is generally believed that expanded (GAA)n repeats block transcription elongation, fine mechanisms responsible for gene repression are not...

journal_title：Nucleic acids research

authors： Krasilnikova MM,Kireeva ML,Petrovic V,Knijnikova N,Kashlev M,Mirkin SM

更新日期：2007-01-01 00:00:00

abstract：:The exact sites at which a number of drugs inhibit the nick translation of DNA by E.coli DNA polymerase-I have been pinpointed. In order to do this, a method has been developed for sequencing double-stranded plasmid DNA from the site of a specifically induced nick. The initial experiments have concentrated on analysis...

journal_title：Nucleic acids research

authors： Wilkins RJ

更新日期：1982-11-25 00:00:00

abstract：:The human thrombopoietin (TPO) gene, which codes for the principal cytokine involved in platelet maturation, shows a peculiar alternative splicing of its last exon, where an intra-exonic 116 nt alternative intron is spliced out in a fraction of its mRNA. To characterize the molecular mechanism underlying this alternat...

journal_title：Nucleic acids research

authors： Romano M,Marcucci R,Baralle FE

更新日期：2001-02-15 00:00:00

abstract：:The recent publication of the Caenorhabditis elegans cisRED database has provided an extensive catalog of upstream elements that are conserved between nematode genomes. We have performed a secondary analysis to determine which subsequences of the cisRED motifs are found in multiple locations throughout the C. elegans ...

journal_title：Nucleic acids research

authors： Sleumer MC,Mah AK,Baillie DL,Jones SJ

更新日期：2010-05-01 00:00:00

abstract：:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...

journal_title：Nucleic acids research

authors： Castroviejo M,Tarragó-Litvak L,Litvak S

更新日期：1975-11-01 00:00:00

abstract：:The RNA Editing Database (REDIdb) is an interactive, web-based database created and designed with the aim to allocate RNA editing events such as substitutions, insertions and deletions occurring in a wide range of organisms. The database contains both fully and partially sequenced DNA molecules for which editing infor...

journal_title：Nucleic acids research

authors： Picardi E,Regina TM,Brennicke A,Quagliariello C

更新日期：2007-01-01 00:00:00

abstract：:The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5'-phosphate (an...

journal_title：Nucleic acids research

authors： Castleman H,Meredith RD,Erlanger BF

更新日期：1980-10-10 00:00:00

abstract：:The heterodimeric subunit, SRP9/14, of the signal recognition particle (SRP) has previously been found to bind to scAlu and scB1 RNAs in vitro and to exist in large excess over SRP in anthropoid cells. Here we show that human and mouse SRP9/14 bind with high affinities to other Alu-like RNAs of different evolutionary ...

journal_title：Nucleic acids research

authors： Bovia F,Wolff N,Ryser S,Strub K

更新日期：1997-01-15 00:00:00

abstract：:SNiPlay is a web-based tool for detection, management and analysis of genetic variants including both single nucleotide polymorphisms (SNPs) and InDels. Version 3 now extends functionalities in order to easily manage and exploit SNPs derived from next generation sequencing technologies, such as GBS (genotyping by sequ...

journal_title：Nucleic acids research

authors： Dereeper A,Homa F,Andres G,Sempere G,Sarah G,Hueber Y,Dufayard JF,Ruiz M

更新日期：2015-07-01 00:00:00

abstract：:The sequence GCGCGCGCGC contains three overlapping, mutually exclusive, BssHII restriction sites, each corresponding to one of the three different reading frames. When inserted into a plasmid and digested with BssHII, the three sites in this sequence are cleaved at an approximate ratio of 2:1:2. Consequently, this sys...

journal_title：Nucleic acids research

authors： Orum H,Poulsen LK

更新日期：1989-04-25 00:00:00

abstract：:Cellular RNA labeling strategies based on bioorthogonal chemical reactions are much less developed in comparison to glycan, protein and DNA due to its inherent instability and lack of effective methods to introduce bioorthogonal reactive functionalities (e.g. azide) into RNA. Here we report the development of a simple...

journal_title：Nucleic acids research

authors： Sawant AA,Tanpure AA,Mukherjee PP,Athavale S,Kelkar A,Galande S,Srivatsan SG

更新日期：2016-01-29 00:00:00

abstract：:Aryldiazonium salts are shown to be useful as phototriggered structural probes for RNA mapping as well as for footprinting of RNA/protein interaction. In particular the yeast tRNA(Asp)/aspartyl-tRNA synthetase complex is shown to involve the variable loop face and the concave side of the L-shaped nucleic acid bound to...

journal_title：Nucleic acids research

authors： Garcia A,Giegé R,Behr JP

更新日期：1990-01-11 00:00:00

abstract：:Single-stranded mRNA molecules form secondary structures through complementary self-interactions. Several hypotheses have been proposed on the relationship between the nucleotide sequence, encoded amino acid sequence and mRNA secondary structure. We performed the first transcriptome-wide in silico analysis of the huma...

journal_title：Nucleic acids research

authors： Shabalina SA,Ogurtsov AY,Spiridonov NA

更新日期：2006-05-08 00:00:00

abstract：:Active DNA demethylation is crucial for epigenetic control, but the underlying enzymatic mechanisms are incompletely understood. REPRESSOR OF SILENCING 1 (ROS1) is a 5-methylcytosine (5-meC) DNA glycosylase/lyase that initiates DNA demethylation in plants through a base excision repair process. The enzyme binds DNA no...

journal_title：Nucleic acids research

authors： Parrilla-Doblas JT,Ponferrada-Marín MI,Roldán-Arjona T,Ariza RR

更新日期：2013-10-01 00:00:00

abstract：:The MIPS Fusarium graminearum Genome Database (FGDB) is a comprehensive genome database on one of the most devastating fungal plant pathogens of wheat and barley. FGDB provides information on two gene sets independently derived by automated annotation of the F.graminearum genome sequence. A complete manually revised g...

journal_title：Nucleic acids research

authors： Güldener U,Mannhaupt G,Münsterkötter M,Haase D,Oesterheld M,Stümpflen V,Mewes HW,Adam G

更新日期：2006-01-01 00:00:00

abstract：:The nucleoside analogs 1-(2'-deoxy-beta-D-ribofuranosyl)- 3-nitropyrrole (9), 1-(2'-deoxy-beta-D-ribofuranosyl)-4-nitropyrazole (10), 1-(2'-deoxy-beta-D-ribofuranosyl)-4-nitroimidazole (11) and 1-(2'-deoxy-beta-D-ribofuranosyl)-5-nitroindole (21) were incorporated into the oligonucleotide 5'-d(CGCXAATTYGCG)-3'in the f...

journal_title：Nucleic acids research

authors： Bergstrom DE,Zhang P,Johnson WT

更新日期：1997-05-15 00:00:00

abstract：:The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric DNA. Herein, we developed an EM-PCR and TA cloning-based approach to s...

journal_title：Nucleic acids research

authors： Zhang X,Zhang Y,Zhang W

更新日期：2020-07-09 00:00:00

abstract：:NMR methods were used to investigate a series of mutants of the pseudoknot within the gene 32 messenger RNA of bacteriophage T2, for the purpose of investigating the range of sequences, stem and loop lengths that can form a similar pseudoknot structure. This information is of particular relevance since the T2 pseudokn...

journal_title：Nucleic acids research

authors： Du Z,Hoffman DW

更新日期：1997-03-15 00:00:00

abstract：:Organisms growing at elevated temperatures face the challenge of maintaining the integrity of their genetic materials. Archaea possess unique chromatin proteins for gene organization and information processing. We present the solution structure of Sso7c4 from Sulfolobus solfataricus, which has a homodimeric DNA-bindin...

journal_title：Nucleic acids research

authors： Hsu CH,Wang AH

更新日期：2011-08-01 00:00:00

abstract：:The J-binding protein 1 (JBP1) is essential for biosynthesis and maintenance of DNA base-J (β-d-glucosyl-hydroxymethyluracil). Base-J and JBP1 are confined to some pathogenic protozoa and are absent from higher eukaryotes, prokaryotes and viruses. We show that JBP1 recognizes J-containing DNA (J-DNA) through a 160-res...

journal_title：Nucleic acids research

authors： Heidebrecht T,Christodoulou E,Chalmers MJ,Jan S,Ter Riet B,Grover RK,Joosten RP,Littler D,van Luenen H,Griffin PR,Wentworth P Jr,Borst P,Perrakis A

更新日期：2011-07-01 00:00:00

abstract：:Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...

journal_title：Nucleic acids research

authors： Zamdborg L,Ma P

更新日期：2009-09-01 00:00:00

abstract：:Polyadenylation is a co-transcriptional process that modifies mRNA 3'-ends in eukaryotes. In yeast, CF IA and CPF constitute the core 3'-end maturation complex. CF IA comprises Rna14p, Rna15p, Pcf11p and Clp1p. CF IA interacts with the C-terminal domain of RNA Pol II largest subunit via Pcf11p which links pre-mRNA 3'-...

journal_title：Nucleic acids research

authors： Haddad R,Maurice F,Viphakone N,Voisinet-Hakil F,Fribourg S,Minvielle-Sébastia L

更新日期：2012-02-01 00:00:00

abstract：:Ethionamide is an antituberculous drug for the treatment of multidrug-resistant Mycobacterium tuberculosis. This antibiotic requires activation by the monooxygenase EthA to exert its activity. Production of EthA is controlled by the transcriptional repressor EthR, a member of the TetR family. The sensitivity of M. tub...

journal_title：Nucleic acids research

authors： Carette X,Blondiaux N,Willery E,Hoos S,Lecat-Guillet N,Lens Z,Wohlkönig A,Wintjens R,Soror SH,Frénois F,Dirié B,Villeret V,England P,Lippens G,Deprez B,Locht C,Willand N,Baulard AR

更新日期：2012-04-01 00:00:00

abstract：:Tunable induction of gene expression is an essential tool in biology and biotechnology. In spite of that, current induction systems often exhibit unpredictable behavior and performance shortcomings, including high sensitivity to transactivator dosage and plasmid take-up variation, and excessive consumption of cellular...

journal_title：Nucleic acids research

authors： Lillacci G,Benenson Y,Khammash M

更新日期：2018-10-12 00:00:00

abstract：:Single-nucleotide polymorphisms (SNPs) are often linked to critical phenotypes such as diseases or responses to vaccines, medications and environmental factors. However, the specific molecular mechanisms by which a causal SNP acts is usually not obvious. Changes in RNA secondary structure emerge as a possible explanat...

journal_title：Nucleic acids research

authors： Salari R,Kimchi-Sarfaty C,Gottesman MM,Przytycka TM

更新日期：2013-01-07 00:00:00