Abstract：
:SV40 DNA form I is expressed efficiently after its injection into the nuclei of Xenopus laevis oocytes, resulting in the synthesis of RNA and protein products of both viral late and early transcription units. However it was observed that injection of SV40 genes cloned into pBR322 or related plasmids yielded vastly reduced quantities of viral DNA and proteins. If SV40 DNA was cleaved from the plasmid, and then recircularized prior to microinjection, viral expression was regained. The inhibition by plasmid DNA was not confined to an effect in cis because coinjection of circular pBR322 DNA along with SV40 DNA, as separate entities, also blocked viral RNA and protein synthesis. As circular but not linear pBR322 DNA was actively transcribed by polymerase II in oocytes, even in the presence of SV40 DNA, it is likely that pBR322 competes for transcription factors required for viral gene expression. Injection of pBR322 as early as two hours after injection of SV40 DNA into the oocyte nucleus did not inhibit SV40 RNA synthesis, indicating that once initiated, SV40 transcription is stable and insensitive to the competition by plasmid DNA. A plasmid vector was developed that allows expression of SV40 DNA in Xenopus laevis oocytes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Michaeli T,Prives Cdoi
10.1093/nar/15.4.1579subject
Has Abstractpub_date
1987-02-25 00:00:00pages
1579-94issue
4eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract：:The current release of ProTherm, Thermodynamic Database for Proteins and Mutants, contains more than 10 000 numerical data (300% of the first version) of several thermodynamic parameters, experimental methods and conditions, reversibility of folding, details about the surrounding residues in space for all mutants, str...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.1.301
更新日期：2002-01-01 00:00:00
abstract：:A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks451
更新日期：2012-08-01 00:00:00
abstract：:We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn359
更新日期：2008-08-01 00:00:00
abstract：:In Saccharomyces cerevisiae, RNA polymerase II assembly is probably initiated by the formation of the RPB3-RPB11 heterodimer. RPB3 is encoded by a single copy gene in the yeast, mouse and human genomes. The RPB11 gene is also unique in yeast and mouse, but in humans a gene family has been identified that potentially e...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki672
更新日期：2005-06-24 00:00:00
abstract：:A C-->U mutation (rdn5) in the conserved sarcin/ricin domain of yeast 25S rRNA has been shown to cause translational suppression and paromomycin resistance. It also separates the killing from the misreading effect of this antibiotic. We confirm these findings and provide in vitro evidence that rdn5 causes a 3-fold inc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh860
更新日期：2004-10-11 00:00:00
abstract：:The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We report here that these transcripts produce three forms of MTH1 polypeptides (...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.22.4335
更新日期：1999-11-15 00:00:00
abstract：:The synthesis of 1-(beta-D-2'-deoxyribosyl)-2-pyrimidinone (dK) and its 5-methyl derivative (d5) from 2'-deoxycytidine or 2'-deoxythymidine, respectively, via silver oxide oxidation of 4-hydrazinopyrimidines is described. The necessary hydrazine substituted pyrimidine nucleosides have been prepared by transamination o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.6.2261
更新日期：1989-03-25 00:00:00
abstract：:The pattern of sites for cleavage mediated by topoisomerase II was determined in 830 kb of cloned DNA from the Drosophila X chromosome, with the objectives of comparing it with mapped structural and functional landmarks and examining if the correlations with such landmarks reported in individual loci can be generalize...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.11.2041
更新日期：1997-06-01 00:00:00
abstract：:Detection of cancer-associated somatic mutations has broad applications for oncology and precision medicine. However, this becomes challenging when cancer-derived DNA is in low abundance, such as in impure tissue specimens or in circulating cell-free DNA. Next-generation sequencing (NGS) is particularly prone to techn...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz474
更新日期：2019-09-05 00:00:00
abstract：:Post-transcriptional modifications are characteristic features of tRNAs and have been shown in a number of cases to influence both their structural and functional properties, including structure stabilization, amino-acylation and codon recognition. We have developed an approach which allows the investigation of the po...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.3.756
更新日期：1999-02-01 00:00:00
abstract：:This study unveils Mycobacterium smegmatis DinB2 as the founder of a clade of Y-family DNA polymerase that is naturally adept at incorporating ribonucleotides by virtue of a leucine in lieu of a canonical aromatic steric gate. DinB2 efficiently scavenges limiting dNTP and rNTP substrates in the presence of manganese. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku752
更新日期：2014-01-01 00:00:00
abstract：:We have developed efficient methods for creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of DNA mapping and sequencing. A novel plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial tran...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.18.3765
更新日期：1994-09-11 00:00:00
abstract：:The function of arginine, cysteine and carboxylic amino acid (glutamic and aspartic) residues of sigma was studied using chemical modification by group specific reagents. Following modification of 3 arginine residues with phenylglyoxal or 3 cysteine residues with N-ethylmaleimide (NEM) sigma activity was lost. Analysi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.9.2701
更新日期：1983-05-11 00:00:00
abstract：:The Gene Expression Atlas (http://www.ebi.ac.uk/gxa) is an added-value database providing information about gene expression in different cell types, organism parts, developmental stages, disease states, sample treatments and other biological/experimental conditions. The content of this database derives from curation, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp936
更新日期：2010-01-01 00:00:00
abstract：:This work presents a neutral filter elution method for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay will detect the number of double strand breaks induced by as little as 1000 rad of X-ray. The rate of DNA elution through the filters under neutral conditions increases with X-ray dose....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.3.793
更新日期：1979-10-10 00:00:00
abstract：:PIECE (Plant Intron Exon Comparison and Evolution) is a web-accessible database that houses intron and exon information of plant genes. PIECE serves as a resource for biologists interested in comparing intron-exon organization and provides valuable insights into the evolution of gene structure in plant genomes. Recent...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw935
更新日期：2017-01-04 00:00:00
abstract：:Extensive research has characterized distinct exogenous RNAi pathways interfering in gene expression during vegetative growth of the unicellular model ciliate Paramecium. However, role of RNAi in endogenous transcriptome regulation, and environmental adaptation is unknown. Here, we describe the first genome-wide profi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz553
更新日期：2019-09-05 00:00:00
abstract：:We have compiled the DNA sequence data for E. coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the fifth listing replacing and increasing the former listings substantially. However, in order to save space this printed version contains...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.13.2973
更新日期：1993-07-01 00:00:00
abstract：:RNA polymerase III recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo(rU) synthesis within this sequence, and releases the RNA. The pol III subunit Rpc11p (C11) mediates RNA 3'-5' cleavage in the catalytic center of pol III during pausing. The amino and carboxyl region...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr182
更新日期：2011-08-01 00:00:00
abstract：:A mathematical model and a computer simulation were used to study PCR specificity. The model describes the occurrences of non-targeted PCR products formed through random primer-template interactions. The PCR simulation scans DNA sequence databases with primers pairs. According to the model prediction, PCR with complex...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.18.3538
更新日期：1996-09-15 00:00:00
abstract：:In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.8.999
更新日期：1974-08-01 00:00:00
abstract：:Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE elements were hybr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.21.4427
更新日期：1997-11-01 00:00:00
abstract：:Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzym...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.3.679
更新日期：1978-03-01 00:00:00
abstract：:Recent progress in predicting RNA structure is moving towards filling the 'gap' in 2D RNA structure prediction where, for example, predicted internal loops often form non-canonical base pairs. This is increasingly recognized with the steady increase of known RNA 3D modules. There is a general interest in matching stru...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt795
更新日期：2013-12-01 00:00:00
abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq823
更新日期：2011-02-01 00:00:00
abstract：:The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bact...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1075
更新日期：2013-12-01 00:00:00
abstract：:The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1302
更新日期：2018-03-16 00:00:00
abstract：:The erbAalpha gene encodes two alpha-thyroid hormone receptor isoforms, TRalpha1 and TRalpha2, which arise from alternatively processed mRNAs, erbAalpha1 (alpha1) and erb alpha2 (alpha2). The splicing and alternative polyadenylation patterns of these mRNAs resemble that of mRNAs encoding different forms of immunoglobu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.21.4296
更新日期：1997-11-01 00:00:00
abstract：:HAP1, also known as APE/Ref-1, is the major apurinic/apyrimidinic (AP) endonuclease in human cells. Previous structural studies have suggested a possible role for the Asp-210 residue of HAP1 in the enzymatic function of this enzyme. Here, we demonstrate that substitution of Asp-210 by Asn or Ala eliminates the AP endo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.11.2207
更新日期：2000-06-01 00:00:00
abstract：:The RNA-Seq technology has revolutionized transcriptome characterization not only by accurately quantifying gene expression, but also by the identification of novel transcripts like chimeric fusion transcripts. The 'fusion' or 'chimeric' transcripts have improved the diagnosis and prognosis of several tumors, and have...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx315
更新日期：2017-07-27 00:00:00
