The synthesis of 2-pyrimidinone nucleosides and their incorporation into oligodeoxynucleotides.

abstract：:The complete nucleotide sequence of the mitochondrial DNA of the amphioxus Branchiostoma lanceolatum has been determined. This mitochondrial genome is small (15 076 bp) because of the short size of the two rRNA genes and the tRNA genes. In addition, this genome contains a very short non-coding region (57 bp) with no s...

journal_title：Nucleic acids research

authors： Spruyt N,Delarbre C,Gachelin G,Laudet V

更新日期：1998-07-01 00:00:00

abstract：:The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric DNA. Herein, we developed an EM-PCR and TA cloning-based approach to s...

journal_title：Nucleic acids research

authors： Zhang X,Zhang Y,Zhang W

更新日期：2020-07-09 00:00:00

abstract：:Human Hepatitis B Virus (HBV) RNAs contain a cis -acting sequence, the post-transcriptional regulatory element (HPRE), which facilitates the cytoplasmic localization of intronless transcripts. Our previous studies have shown that the HPRE is composed of at least two independent sub-elements, HPREalpha and HPREbeta, wh...

journal_title：Nucleic acids research

authors： Smith GJ 3rd,Donello JE,Lück R,Steger G,Hope TJ

更新日期：1998-11-01 00:00:00

abstract：:Brome mosaic virus (BMV) is a tripartite genome, positive-sense RNA virus of plants. Previously it was demonstrated that local hybridization between BMV RNAs (RNA-RNA heteroduplex formation) efficiently promotes non-homologous RNA recombination. In addition, studies on the role of the BMV polymerase in RNA recombinati...

journal_title：Nucleic acids research

authors： Figlerowicz M

更新日期：2000-04-15 00:00:00

abstract：:To date, an effective therapeutic treatment that confers strong attenuation toward coronaviruses (CoVs) remains elusive. Of all the potential drug targets, the helicase of CoVs is considered to be one of the most important. Here, we first present the structure of the full-length Nsp13 helicase of SARS-CoV (SARS-Nsp13)...

journal_title：Nucleic acids research

authors： Jia Z,Yan L,Ren Z,Wu L,Wang J,Guo J,Zheng L,Ming Z,Zhang L,Lou Z,Rao Z

更新日期：2019-07-09 00:00:00

abstract：:DNA bearing lambda operator sequences was methylated by dimethyl sulfate (DMS) in the presence or absence of lambda repressor. Under the experimental conditions, DMS methylates only the purine residues. The presence of lambda repressor affects only the methylation of certain G residues in the operators. Repressor bloc...

journal_title：Nucleic acids research

authors： Humayun Z,Kleid D,Ptashne M

更新日期：1977-01-01 00:00:00

abstract：:Heat shock genes are activated by the binding of the heat shock transcription factor (HSF) to heat shock elements (HSEs), consisting of arrays of the 5-bp unit NGAAN arranged as inverted repeats. Here, we have investigated the interaction of the 5-bp unit with HSFs of Drosophila and Saccharomyces. Mutations within the...

journal_title：Nucleic acids research

authors： Fernandes M,Xiao H,Lis JT

更新日期：1994-01-25 00:00:00

abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...

journal_title：Nucleic acids research

authors： Wang XC,Wilson SC,Hammond MC

更新日期：2016-09-30 00:00:00

abstract：:The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains sev...

journal_title：Nucleic acids research

authors： Ledeboer AM,Edens L,Maat J,Visser C,Bos JW,Verrips CT,Janowicz Z,Eckart M,Roggenkamp R,Hollenberg CP

更新日期：1985-05-10 00:00:00

abstract：:The Central glycolytic genes Repressor (CggR) from Bacillus subtilis belongs to the SorC family of transcription factors that control major carbohydrate metabolic pathways. Recent studies have shown that CggR binds as a tetramer to its tandem operator DNA sequences and that the inducer metabolite, fructose 1,6-bisphos...

journal_title：Nucleic acids research

authors： Chaix D,Ferguson ML,Atmanene C,Van Dorsselaer A,Sanglier-Cianférani S,Royer CA,Declerck N

更新日期：2010-09-01 00:00:00

abstract：:Genome organization into transcriptionally active domains denotes one of the first levels of gene expression regulation. Although the chromatin domain concept is generally accepted, only little is known on how domain organization impacts the regulation of differential gene expression. Insulators might hold answers to ...

journal_title：Nucleic acids research

authors： Furlan-Magaril M,Rebollar E,Guerrero G,Fernández A,Moltó E,González-Buendía E,Cantero M,Montoliu L,Recillas-Targa F

更新日期：2011-01-01 00:00:00

abstract：:Monocrotaline is a pyrrolizidine alkaloid known to cause toxicity in humans and animals. Its mechanism of biological action is still unclear although DNA crosslinking has been suggested to a play a role in its activity. In this study we found that an active metabolite of monocrotaline, dehydromonocrotaline (DHM), alky...

journal_title：Nucleic acids research

authors： Pereira TN,Webb RI,Reilly PE,Seawright AA,Prakash AS

更新日期：1998-12-01 00:00:00

abstract：:Denaturing gradient gel electrophoresis (DGGE) is based upon the different melting behaviors of DNA molecules in a chemical denaturant gradient according to their sequences. This technique has recently become a widespread tool to detect mutations. The introduction of a GC-clamp enables the detection of most single bas...

journal_title：Nucleic acids research

authors： Weber CK,Shaffer DJ,Sidman CL

更新日期：1991-06-25 00:00:00

abstract：:Stabilization of stalled replication forks prevents excessive fork reversal or degradation, which can undermine genome integrity. The WRN protein is unique among the other human RecQ family members to possess exonuclease activity. However, the biological role of the WRN exonuclease is poorly defined. Recently, the WRN...

journal_title：Nucleic acids research

authors： Aiello FA,Palma A,Malacaria E,Zheng L,Campbell JL,Shen B,Franchitto A,Pichierri P

更新日期：2019-07-26 00:00:00

abstract：:RNA is emerging as a key regulator of a plethora of biological processes. While its study has remained elusive for decades, the recent advent of high-throughput sequencing technologies provided the unique opportunity to develop novel techniques for the study of RNA structure and post-transcriptional modifications. Non...

journal_title：Nucleic acids research

authors： Incarnato D,Morandi E,Simon LM,Oliviero S

更新日期：2018-09-19 00:00:00

abstract：:Antigenic variation of the pilus is an essential component of Neisseria gonorrhoeae pathogenesis. Unidirectional recombination of silent pilin DNA into an expressed pilin gene allows for substantial sequence variation of this highly immunogenic surface structure. While the RecA protein is required for pilin gene recom...

journal_title：Nucleic acids research

authors： Wainwright LA,Frangipane JV,Seifert HS

更新日期：1997-04-01 00:00:00

abstract：:IntAct is an open-source, open data molecular interaction database populated by data either curated from the literature or from direct data depositions. Two levels of curation are now available within the database, with both IMEx-level annotation and less detailed MIMIx-compatible entries currently supported. As from ...

journal_title：Nucleic acids research

authors： Kerrien S,Aranda B,Breuza L,Bridge A,Broackes-Carter F,Chen C,Duesbury M,Dumousseau M,Feuermann M,Hinz U,Jandrasits C,Jimenez RC,Khadake J,Mahadevan U,Masson P,Pedruzzi I,Pfeiffenberger E,Porras P,Raghunath A,Roeche

更新日期：2012-01-01 00:00:00

abstract：:The alcohol dehydrogenase (Adh) gene of D. melanogaster is transcribed from two different promoters during fly development: the distal (adult) and the proximal (embryonic-larval). Certain aspects of Adh gene regulation are represented in Drosophila continuous cell lines. We have used Drosophila tissue culture cells in...

journal_title：Nucleic acids research

authors： Benyajati C,Ayer S,McKeon J,Ewel A,Huang J

更新日期：1987-10-12 00:00:00

abstract：:The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases ind...

journal_title：Nucleic acids research

authors： Grandgenett DP,Vora AC

更新日期：1985-09-11 00:00:00

abstract：:We have analyzed the molecular mechanism that makes translation of the MS2 replicase cistron dependent on the translation of the upstream coat cistron. Deletion mapping on cloned cDNA of the phage shows that the ribosomal binding site of the replicase cistron is masked by a long distance basepairing to an internal coa...

journal_title：Nucleic acids research

authors： Berkhout B,van Duin J

更新日期：1985-10-11 00:00:00

abstract：:In Saccharomyces cerevisiae, RNA polymerase II assembly is probably initiated by the formation of the RPB3-RPB11 heterodimer. RPB3 is encoded by a single copy gene in the yeast, mouse and human genomes. The RPB11 gene is also unique in yeast and mouse, but in humans a gene family has been identified that potentially e...

journal_title：Nucleic acids research

authors： Benga WJ,Grandemange S,Shpakovski GV,Shematorova EK,Kedinger C,Vigneron M

更新日期：2005-06-24 00:00:00

abstract：:A comparison of gene structure, sequence, and transcription pattern of heat shock loci 93D of Drosophila melanogaster and 48B of Drosophila hydei has been performed. Both heat shock loci consist of an unique region that is flanked by an internally repetitive element. Different members of these elements are highly cons...

journal_title：Nucleic acids research

authors： Ryseck RP,Walldorf U,Hoffmann T,Hovemann B

更新日期：1987-04-24 00:00:00

abstract：:Protein p56 (56 amino acids) from the Bacillus subtilis phage 29 inactivates the host uracil-DNA glycosylase (UDG), an enzyme involved in the base excision repair pathway. At present, p56 is the only known example of a UDG inhibitor encoded by a non-uracil containing viral DNA. Using analytical ultracentrifugation met...

journal_title：Nucleic acids research

authors： Serrano-Heras G,Ruiz-Masó JA,del Solar G,Espinosa M,Bravo A,Salas M

更新日期：2007-01-01 00:00:00

abstract：:The Saccharomyces cerevisiae Sgs1p helicase localizes to the nucleolus and is required to maintain the integrity of the rDNA repeats. Sgs1p is a member of the RecQ DNA helicase family, which also includes Schizo-saccharomyces pombe Rqh1, and the human BLM and WRN genes. These genes encode proteins which are essential ...

journal_title：Nucleic acids research

authors： Sun H,Bennett RJ,Maizels N

更新日期：1999-05-01 00:00:00

abstract：:Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light microscopic particle observation is usually limited to two-dimensional projections of short trajectory segments, we report here imag...

journal_title：Nucleic acids research

authors： Spille JH,Kaminski TP,Scherer K,Rinne JS,Heckel A,Kubitscheck U

更新日期：2015-01-01 00:00:00

abstract：:Triethylammonium uridine-3',5'-cyclic phosphorothioate crystallizes in space group P2(1)2(1)2(1), a = 7.177(1), b = 13.155(6), c = 21.114(7) A, C15H26N3O7PS, MW 423.4, Z = 4, dx = 1.41g/cm3. The crystal structure was solved by direct methods on the basis of 1493 counter X-ray diffraction data (CuK alpha) and refined t...

journal_title：Nucleic acids research

authors： Hinrichs W,Steifa M,Saenger W,Eckstein F

更新日期：1987-06-25 00:00:00

abstract：:To enhance our knowledge regarding biological pathway regulation, we took an integrated approach, using the biomedical literature, ontologies, network analyses and experimental investigation to infer novel genes that could modulate biological pathways. We first constructed a novel gene network via a pairwise compariso...

journal_title：Nucleic acids research

authors： Qin T,Matmati N,Tsoi LC,Mohanty BK,Gao N,Tang J,Lawson AB,Hannun YA,Zheng WJ

更新日期：2014-10-01 00:00:00

abstract：:A tetradecanucleotide U-A-G-C(U-C-G)2G-G-C-Up corresponding to bases 21-34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of chemically synthesized oligonucleotides: 1) U-A-G-C + U-C-G-U-C-G + G-G-C-Up and 2) U-A-G-C + U-C-G-U + C-G-G-G-C-Up. In reactio...

journal_title：Nucleic acids research

authors： Ohtsuka E,Doi T,Uemura H,Taniyama Y,Ikehara M

更新日期：1980-09-11 00:00:00

abstract：:Integrating DNA delivery systems hold promise for many applications including treatment of diseases; however, targeted integration is needed for improved safety. The piggyBac (PB) transposon system is a highly active non-viral gene delivery system capable of integrating defined DNA segments into host chromosomes witho...

journal_title：Nucleic acids research

authors： Luo W,Galvan DL,Woodard LE,Dorset D,Levy S,Wilson MH

更新日期：2017-08-21 00:00:00

abstract：:The hydroxyl radical is a powerful oxidant that generates DNA lesions including the stereoisomeric R and S 5',8-cyclo-2'-deoxyadenosine (cdA) and 5',8-cyclo-2'-deoxyguanosine (cdG) pairs that have been detected in cellular DNA. Unlike some other oxidatively generated DNA lesions, cdG and cdA are repaired by the human ...

journal_title：Nucleic acids research

authors： Kropachev K,Ding S,Terzidis MA,Masi A,Liu Z,Cai Y,Kolbanovskiy M,Chatgilialoglu C,Broyde S,Geacintov NE,Shafirovich V

更新日期：2014-04-01 00:00:00