Abstract:
:We present a tool for the prediction of mRNA 3'-processing (cleavage and polyadenylation) sites in the yeast Saccharomyces cerevisiae, based on a discrete state-space model or hidden Markov model. Comparison of predicted sites with experimentally verified 3'-processing sites indicates good agreement. All predicted or known yeast genes were analyzed to find probable 3'-processing sites. Known alternative 3'-processing sites, both within the 3'-untranslated region and within the protein coding sequence were successfully identified, leading to the possibility of prediction of previously unknown alternative sites. The lack of an apparent 3'-processing site calls into question the validity of some predicted genes. This is specifically investigated for predicted genes with overlapping coding sequences.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Graber JH,McAllister GD,Smith TFdoi
10.1093/nar/30.8.1851keywords:
subject
Has Abstractpub_date
2002-04-15 00:00:00pages
1851-8issue
8eissn
0305-1048issn
1362-4962journal_volume
30pub_type
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