Probabilistic prediction of Saccharomyces cerevisiae mRNA 3'-processing sites.

Abstract:

:We present a tool for the prediction of mRNA 3'-processing (cleavage and polyadenylation) sites in the yeast Saccharomyces cerevisiae, based on a discrete state-space model or hidden Markov model. Comparison of predicted sites with experimentally verified 3'-processing sites indicates good agreement. All predicted or known yeast genes were analyzed to find probable 3'-processing sites. Known alternative 3'-processing sites, both within the 3'-untranslated region and within the protein coding sequence were successfully identified, leading to the possibility of prediction of previously unknown alternative sites. The lack of an apparent 3'-processing site calls into question the validity of some predicted genes. This is specifically investigated for predicted genes with overlapping coding sequences.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Graber JH,McAllister GD,Smith TF

doi

10.1093/nar/30.8.1851

keywords:

subject

Has Abstract

pub_date

2002-04-15 00:00:00

pages

1851-8

issue

8

eissn

0305-1048

issn

1362-4962

journal_volume

30

pub_type

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