A weak interaction between the U2A' protein and U2 snRNA helps to stabilize their complex with the U2B" protein.

abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...

journal_title：Nucleic acids research

authors： Saintomé C,Amrane S,Mergny JL,Alberti P

更新日期：2016-04-07 00:00:00

abstract：:Vaccinia virus DNA topoisomerase catalyzes resolution of synthetic Holliday junctions in vitro. The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/, that are opposed within a partially mobile four-way junction. Efficient resolution occurs on a junction with a 10 bp segment of branc...

journal_title：Nucleic acids research

authors： Sekiguchi J,Cheng C,Shuman S

更新日期：2000-07-15 00:00:00

abstract：:The effect of [Na+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stabili...

journal_title：Nucleic acids research

authors： Burnett JP,Frank BH,Douthart RJ

更新日期：1975-06-01 00:00:00

abstract：:Chemically modified antisense oligonucleotides (ASOs) with phosphorothioate (PS) linkages have been extensively studied as research and therapeutic agents. PS-ASOs can enter the cell and trigger cleavage of complementary RNA by RNase H1 even in the absence of transfection reagent. A number of cell surface proteins hav...

journal_title：Nucleic acids research

authors： Wang S,Allen N,Vickers TA,Revenko AS,Sun H,Liang XH,Crooke ST

更新日期：2018-04-20 00:00:00

abstract：:Interaction detection methods have led to the discovery of thousands of interactions between proteins, and discerning relevance within large-scale data sets is important to present-day biology. Here, a spectral method derived from graph theory was introduced to uncover hidden topological structures (i.e. quasi-cliques...

journal_title：Nucleic acids research

authors： Bu D,Zhao Y,Cai L,Xue H,Zhu X,Lu H,Zhang J,Sun S,Ling L,Zhang N,Li G,Chen R

更新日期：2003-05-01 00:00:00

abstract：:Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIα interacts with specific RNA polymerases and chromatin-remodeling complexes...

journal_title：Nucleic acids research

authors： Nikolaou C,Bermúdez I,Manichanh C,García-Martinez J,Guigó R,Pérez-Ortín JE,Roca J

更新日期：2013-11-01 00:00:00

abstract：:We present a www server for AUGUSTUS, a novel software program for ab initio gene prediction in eukaryotic genomic sequences. Our method is based on a generalized Hidden Markov Model with a new method for modeling the intron length distribution. This method allows approximation of the true intron length distribution m...

journal_title：Nucleic acids research

authors： Stanke M,Steinkamp R,Waack S,Morgenstern B

更新日期：2004-07-01 00:00:00

abstract：:The interaction of RNase T1 with calf thymus DNA was studied using uv difference spectroscopy and the effect of the enzyme on DNA melting. There was no indication of RNase T1 binding with native DNA. A prominent difference spectrum for RNase T1 binding with denatured DNA (d-DNA) was observed at pH 5, 25 degrees and lo...

journal_title：Nucleic acids research

authors： Walz FG,Biddlecome S,Hooverman L

更新日期：1975-01-01 00:00:00

abstract：:The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5'-phosphate (an...

journal_title：Nucleic acids research

authors： Castleman H,Meredith RD,Erlanger BF

更新日期：1980-10-10 00:00:00

abstract：:The regulation of the Sso II restriction-modification system from Shigella sonnei was studied in vivo and in vitro . In lacZ fusion experiments, Sso II methyltransferase (M. Sso II) was found to repress its own synthesis but stimulate expression of the cognate restriction endonuclease (ENase). The N-terminal 72 amino ...

journal_title：Nucleic acids research

authors： Karyagina A,Shilov I,Tashlitskii V,Khodoun M,Vasil'ev S,Lau PC,Nikolskaya I

更新日期：1997-06-01 00:00:00

abstract：:The products of the RAG-1 and RAG-2 genes are essential for the recombination of the DNA encoding the antigen receptors of the developing immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing loca...

journal_title：Nucleic acids research

authors： Sadofsky MJ,Hesse JE,McBlane JF,Gellert M

更新日期：1993-12-11 00:00:00

abstract：:The Network of Cancer Genes (NCG) collects and integrates data on 736 human genes that are mutated in various types of cancer. For each gene, NCG provides information on duplicability, orthology, evolutionary appearance and topological properties of the encoded protein in a comprehensive version of the human protein-p...

journal_title：Nucleic acids research

authors： Syed AS,D'Antonio M,Ciccarelli FD

更新日期：2010-01-01 00:00:00

abstract：:The availability of protein fluorophores with appropriate spectral properties has made it possible to employ fluorescence resonance energy transfer (FRET) to assess interactions between three proteins microscopically. Flow cytometry offers excellent sensitivity, effective signal separation and the capacity to assess a...

journal_title：Nucleic acids research

authors： He L,Wu X,Simone J,Hewgill D,Lipsky PE

更新日期：2005-04-01 00:00:00

abstract：:We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a s...

journal_title：Nucleic acids research

authors： Yu BJ,Kang KH,Lee JH,Sung BH,Kim MS,Kim SC

更新日期：2008-08-01 00:00:00

abstract：:Translation initiation of eukaryotic mRNAs generally occurs by cap-dependent ribosome scanning. However, certain mRNAs contain internal ribosome entry sites (IRES) allowing cap-independent translation. Several of these IRES-competent transcripts and their corresponding proteins are involved in tumourigenesis. This stu...

journal_title：Nucleic acids research

authors： Petz M,Kozina D,Huber H,Siwiec T,Seipelt J,Sommergruber W,Mikulits W

更新日期：2007-01-01 00:00:00

abstract：:Crotonaldehyde is a representative alpha,beta-unsaturated aldehyde endowed of mutagenic and carcinogenic properties related to its propensity to react with DNA. Cyclic crotonaldehyde-derived deoxyguanosine (CrA-PdG) adducts can undergo ring opening in duplex DNA to yield a highly reactive aldehydic moiety. Here, we de...

journal_title：Nucleic acids research

authors： Dexheimer TS,Kozekova A,Rizzo CJ,Stone MP,Pommier Y

更新日期：2008-07-01 00:00:00

abstract：:RNA is emerging as a key regulator of a plethora of biological processes. While its study has remained elusive for decades, the recent advent of high-throughput sequencing technologies provided the unique opportunity to develop novel techniques for the study of RNA structure and post-transcriptional modifications. Non...

journal_title：Nucleic acids research

authors： Incarnato D,Morandi E,Simon LM,Oliviero S

更新日期：2018-09-19 00:00:00

abstract：:A comprehensive listing is made of posttranscriptionally modified nucleosides from RNA reported in the literature through mid-1994. Included are chemical structures, common names, symbols, Chemical Abstracts registry numbers (for ribonucleoside and corresponding base), Chemical Abstracts Index Name, phylogenetic sourc...

journal_title：Nucleic acids research

authors： Limbach PA,Crain PF,McCloskey JA

更新日期：1994-06-25 00:00:00

abstract：:In this report, we provide an update on the services and content which are available on the web server of IBM's Bioinformatics and Pattern Discovery group. The server, which is operational around the clock, provides access to a large number of methods that have been developed and published by the group's members. Ther...

journal_title：Nucleic acids research

authors： Huynh T,Rigoutsos I

更新日期：2004-07-01 00:00:00

abstract：:Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Ini...

journal_title：Nucleic acids research

authors： Zhang ZY,Thompson EA,Stallcup MR

更新日期：1984-11-12 00:00:00

abstract：:Human transcription factor IIIC contains a 55 kDa polypeptide which specifically interacts with the Adenovirus 2 VAI gene promoter and which mimics most of the DNA binding properties of the entire factor. The specificity and affinity of this protein:DNA interaction was demonstrated by: (i) Separation of purified fract...

journal_title：Nucleic acids research

authors： Schneider HR,Waldschmidt R,Seifart KH

更新日期：1990-08-25 00:00:00

abstract：:The role of Ribonuclease H1 (RNase H1) during primer removal and ligation at the mitochondrial origin of light-strand DNA synthesis (OriL) is a key, yet poorly understood, step in mitochondrial DNA maintenance. Here, we reconstitute the replication cycle of L-strand synthesis in vitro using recombinant mitochondrial p...

journal_title：Nucleic acids research

authors： Al-Behadili A,Uhler JP,Berglund AK,Peter B,Doimo M,Reyes A,Wanrooij S,Zeviani M,Falkenberg M

更新日期：2018-10-12 00:00:00

abstract：:The Diels-Alder reaction between diene-modified oligonucleotides and maleimide-derivatized peptides afforded peptide-oligonucleotide conjugates with high purity and yield. Synthesis of the reagents was easily accomplished by on-column derivatization of the corresponding peptides and oligonucleotides. The cycloaddition...

journal_title：Nucleic acids research

authors： Marchán V,Ortega S,Pulido D,Pedroso E,Grandas A

更新日期：2006-02-14 00:00:00

abstract：:Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-strande...

journal_title：Nucleic acids research

authors： Seidl CI,Lama L,Ryan K

更新日期：2013-02-01 00:00:00

abstract：:Homozygosity for a natural deletion variant of the HIV-coreceptor molecule CCR5, CCR5Δ32, confers resistance toward HIV infection. Allogeneic stem cell transplantation from a CCR5Δ32-homozygous donor has resulted in the first cure from HIV ('Berlin patient'). Based thereon, genetic disruption of CCR5 using designer nu...

journal_title：Nucleic acids research

authors： Mock U,Machowicz R,Hauber I,Horn S,Abramowski P,Berdien B,Hauber J,Fehse B

更新日期：2015-06-23 00:00:00

abstract：:The heterodimeric subunit, SRP9/14, of the signal recognition particle (SRP) has previously been found to bind to scAlu and scB1 RNAs in vitro and to exist in large excess over SRP in anthropoid cells. Here we show that human and mouse SRP9/14 bind with high affinities to other Alu-like RNAs of different evolutionary ...

journal_title：Nucleic acids research

authors： Bovia F,Wolff N,Ryser S,Strub K

更新日期：1997-01-15 00:00:00

abstract：:In vitro selection of RNA-cleaving DNAzymes is a powerful method for isolating metal-specific DNA. A few successful examples are known, but it is still difficult to target some thiophilic metals such as Cd(2+) due to limited functional groups in DNA. While using modified bases expands the chemical functionality of DNA...

journal_title：Nucleic acids research

authors： Huang PJ,Liu J

更新日期：2015-07-13 00:00:00

abstract：:The interaction of the high mobility group proteins, HMG14 and HMG17, with nucleosome core particles has been studied. The results show that two molecules of HMG14/17 can be bound tightly but reversibly to each core particle and that their affinity for core particles is greater than their affinity for histone-free DNA...

journal_title：Nucleic acids research

authors： Sandeen G,Wood WI,Felsenfeld G

更新日期：1980-09-11 00:00:00

abstract：:Primer design is a fundamental technique that is widely used for polymerase chain reaction (PCR). Although many methods have been proposed for primer design, they require a great deal of manual effort to generate feasible and valid primers, including homology tests on off-target sequences using BLAST-like tools. That ...

journal_title：Nucleic acids research

authors： Kim H,Kang N,Chon KW,Kim S,Lee N,Koo J,Kim MS

更新日期：2015-11-16 00:00:00

abstract：:Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuri...

journal_title：Nucleic acids research

authors： Shen L,Gao G,Zhang Y,Zhang H,Ye Z,Huang S,Huang J,Kang J

更新日期：2010-10-01 00:00:00