Abstract:
:A synthetic gene for somatomedin C, a human growth factor, has been assembled by a single ligation of 23 oligodeoxyribonucleotides, which were chemically synthesized by an improved solid phase phosphotriester method.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sproat BS,Gait MJdoi
10.1093/nar/13.8.2959subject
Has Abstractpub_date
1985-04-25 00:00:00pages
2959-77issue
8eissn
0305-1048issn
1362-4962journal_volume
13pub_type
杂志文章abstract::Simian cells have been transformed with SV40 origin-defective recombinant plasmids containing the tsA209 T-antigen gene. These plasmids contain deletions of either 5 or 52 nucleotides that include the BglI site at the SV40 ori, are defective for replication in COS-1 cells but retain a functional SV40 early promoter. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.22.7913
更新日期:1985-11-25 00:00:00
abstract::The biogenesis of ribosomes in vivo is an essential process for cellular functions. Transcription of ribosomal RNA (rRNA) genes is the rate-limiting step in ribosome biogenesis controlled by environmental conditions. Here, we investigated the role of folate antagonist on changes of DNA double-strand breaks (DSBs) land...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1208
更新日期:2017-03-17 00:00:00
abstract::The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm817
更新日期:2007-01-01 00:00:00
abstract::The genome stability is maintained by coordinated action of DNA repairs and checkpoints, which delay progression through the cell cycle in response to DNA damage. Rad9 is conserved from yeast to human and functions in cell cycle checkpoint controls. Here, a regulatory mechanism for Rad9 function is reported. In this s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1264
更新日期:2011-06-01 00:00:00
abstract::We have prepared cDNA libraries for amoebae and plasmodia of the acellular slime mould, Physarum polycephalum. Differential screening was used to isolate cell-type-specific cDNA clones (in bacteriophage M13) and both libraries yielded approximately 5% of such sequences. The amoebal- and plasmodial-specific clones were...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.3.933
更新日期:1987-02-11 00:00:00
abstract::Given the many roles proposed for Mg2+ in nucleic acids, it is essential to accurately determine their binding modes. Here, we surveyed the PDB to classify Mg2+ inner-sphere binding patterns to nucleobase imine N1/N3/N7 atoms. Among those, purine N7 atoms are considered to be the best nucleobase binding sites for diva...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1175
更新日期:2017-01-25 00:00:00
abstract::A search of sequence information in the GenBank files shows that tracts of 15-30 contiguous purines are greatly overrepresented in all eukaryotic species examined, ranging from yeast to human. Such an overabundance does not occur in prokaryotic sequences. The large increase in the number of oligopurine tracts cannot b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.4.689
更新日期:1995-02-25 00:00:00
abstract::The stable intermediates formed in the reaction of cytosine, cytidine and adenosine with chloracetaldehyde were isolated. The -CH2CH/OH/- bridge between the exo and endo nitrogen atoms of the parent base was found in these compounds by means of PMR spectroscopy. Their acid-induced dehydration resulted in formation of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.3.789
更新日期:1978-03-01 00:00:00
abstract::During erythropoiesis in the chick, the nucleosome repeat length increases from 190 base pairs to 212 base pairs. This increase is correlated with a dramatic increase in the concentration of the red cell specific histone H5 (from 0.2 molecules per nucleosome to 1 molecule per nucleosome) and with no change in the conc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.4.1179
更新日期:1978-04-01 00:00:00
abstract::Genomic DNA methylation contributes substantively to transcriptional regulations that underlie mammalian development and cellular differentiation. Much effort has been made to decipher the molecular mechanisms governing the establishment and maintenance of DNA methylation patterns. However, little is known about genom...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr017
更新日期:2011-05-01 00:00:00
abstract::The majority of genome-wide association study (GWAS) risk variants reside in non-coding DNA sequences. Understanding how these sequence modifications lead to transcriptional alterations and cell-to-cell variability can help unraveling genotype-phenotype relationships. Here, we describe a computational method, dubbed C...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1263
更新日期:2017-03-17 00:00:00
abstract::We previously showed that histone H4 serine-1 phosphorylation (H4S1ph) is evolutionarily conserved during gametogenesis, and contributes to post-meiotic nuclear compaction and to full completion of sporulation in the yeast Saccharomyces cerevisiae. Previous studies showed that H4S1ph and another modification of the sa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq218
更新日期:2010-08-01 00:00:00
abstract::PrimPol is a primase-polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been established. Here, we report that the proposed zinc finger domain ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku214
更新日期:2014-05-01 00:00:00
abstract::Signal Transducers and Activators of Transcription (STAT) 5A/B regulate cytokine-inducible genes upon binding to GAS motifs. It is not known what percentage of genes with GAS motifs bind to and are regulated by STAT5. Moreover, it is not clear whether genome-wide STAT5 binding is modulated by its concentration. To cla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks056
更新日期:2012-05-01 00:00:00
abstract::Vaccinia virus DNA topoisomerase catalyzes resolution of synthetic Holliday junctions in vitro. The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/, that are opposed within a partially mobile four-way junction. Efficient resolution occurs on a junction with a 10 bp segment of branc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.14.2658
更新日期:2000-07-15 00:00:00
abstract::The antigene strategy based on site-specific recognition of duplex DNA by triplex DNA formation has been exploited in a wide range of biological activities. However, specific triplex formation is mostly restricted to homo-purine strands within the target duplex DNA, due to the destabilizing effect of CG and TA inversi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky704
更新日期:2018-09-28 00:00:00
abstract::The multi-protein complex WRAD, formed by WDR5, RbBP5, Ash2L and Dpy30, binds to the MLL SET domain to stabilize the catalytically active conformation required for histone H3K4 methylation. In addition, the WRAD complex contributes to the targeting of the activated complex to specific sites on chromatin. RbBP5 is cent...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky199
更新日期:2018-04-20 00:00:00
abstract::Accumulating evidence has demonstrated that transcriptional regulation is affected by DNA methylation. Understanding the perturbation of DNA methylation-mediated regulation between transcriptional factors (TFs) and targets is crucial for human diseases. However, the global landscape of DNA methylation-mediated transcr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa041
更新日期:2020-03-18 00:00:00
abstract::Mouse DNA contains two equally abundant, homologous subfamilies of MspI 3.6 and 5 kb repeated fragments. The first subfamily corresponds to the previously described (1) Bam 4 kb repeats, the second one to Bam repeated fragments of higher molecular weight. These subfamilies account for the vast majority of long Bam rep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.3.1593
更新日期:1984-02-10 00:00:00
abstract::Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.4.1251
更新日期:1985-02-25 00:00:00
abstract::The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks198
更新日期:2012-07-01 00:00:00
abstract::DNA restriction fragments located 5' to the human c-myc gene display anomalous electrophoretic mobility on polyacrylamide gels. Computer modeling of the c-myc flanking DNA suggests that the slow-moving DNA fragments spanning nucleotides -1690 to -1054 (relative to c-myc promoter P1) and -718 to -452 form large left ha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.7.2819
更新日期:1989-04-11 00:00:00
abstract::The biological data explosion of the 'omics' era requires fast access to many data types in rapidly growing data banks. The MRS server allows for very rapid queries in a large number of flat-file data banks, such as EMBL, UniProt, OMIM, dbEST, PDB, KEGG, etc. This server combines a fast and reliable backend with a ver...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki422
更新日期:2005-07-01 00:00:00
abstract::Mechanisms that preserve genome integrity are highly important during the normal life cycle of human cells. Loss of genome protective mechanisms can lead to the development of diseases such as cancer. Checkpoint kinases function in the cellular surveillance pathways that help cells to cope with DNA damage. Importantly...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkr697
更新日期:2012-01-01 00:00:00
abstract::Archaeal family-B DNA polymerases bind tightly to deaminated bases and stall replication on encountering uracil in template strands, four bases ahead of the primer-template junction. Should the polymerase progress further towards the uracil, for example, to position uracil only two bases in front of the junction, 3'-5...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1364
更新日期:2013-02-01 00:00:00
abstract::The location of target DNA sequences within chromatin may affect the ability of trans-acting factors to bind cis-elements and regulate gene transcription. To examine the effect of chromatin structure on the ability of the estrogen-estrogen receptor complex (E2R) to bind its respective DNA binding element within the ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.6.1065
更新日期:1996-03-15 00:00:00
abstract::Gene set enrichment testing can enhance the biological interpretation of ChIP-seq data. Here, we develop a method, ChIP-Enrich, for this analysis which empirically adjusts for gene locus length (the length of the gene body and its surrounding non-coding sequence). Adjustment for gene locus length is necessary because ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku463
更新日期:2014-07-01 00:00:00
abstract::Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus RNA 3. The structure consists of two stem-loops separated by a single-stranded region. RNA 3-derived transcripts were ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.23.4723
更新日期:1997-12-01 00:00:00
abstract::HSP90, found in all kingdoms of life, is a major chaperone protein regulating many client proteins. We demonstrated that HSP90α, one of two paralogs duplicated in vertebrates, plays an important role in the biogenesis of fetal PIWI-interacting RNAs (piRNA), which act against the transposon activities, in mouse male ge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku881
更新日期:2014-10-29 00:00:00
abstract::The putative role of double-strand breaks (DSBs) created in vitro by restriction enzyme cleavage in or near CGG*CCG or CTG*CAG repeat tracts on their genetic instabilities, both within the repeats and in their flanking sequences, was investigated in an Escherichia coli plasmid system. DSBs at TRS junctions with the ve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl612
更新日期:2006-01-01 00:00:00