Abstract：
:A typical G-rich telomeric DNA strand, which runs 5'-->3' toward the chromosome ends, protrudes by several nucleotides in lower eukaryotes. In human chromosomes long G-rich 3'-overhangs have been found. Apart from the standard G-rich tail, several non-canonical terminal structures have been proposed. However, the mechanism of long-tail formation, the presence and the role of these structures in telomere maintenance or shortening are not completely understood. In a search for a simple method to accurately measure the 3'-overhang we have established a protocol based on the ligation of telomeric oligonucleotide hybridized to non-denatured DNA under stringent conditions (oligonucleotide ligation assay with telomeric repeat oligonucleotide). This method enabled us to detect a large proportion of G-rich single-stranded telomeric DNA that was as short as 24 nt. Nevertheless, we showed G-tails longer than 400 nt. In all tested cells the lengths ranging from 108 to 270 nt represented only 37% of the whole molecule population, while 56-62% were <90 nt. Our protocol provides a simple and sensitive method for measuring the length of naturally occurring unpaired repeated DNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cimino-Reale G,Pascale E,Battiloro E,Starace G,Verna R,D'Ambrosio Edoi
10.1093/nar/29.7.e35keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
E35issue
7eissn
0305-1048issn
1362-4962journal_volume
29pub_type
杂志文章abstract：:The GeneSpeed database (http://genespeed.uchsc.edu/) is an online database and resource tool facilitating the detailed study of protein domain homology in the transcriptomes of Homo sapiens, Mus musculus, Drosophila melanogaster and Caenorhabditis elegans. The population schema for the GeneSpeed database takes advanta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl990
更新日期：2007-01-01 00:00:00
abstract：:A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.6.1541
更新日期：1979-11-24 00:00:00
abstract：:Chloroplast DNA, isolated from a synchronized culture of Chlamydomonas reinhardii, was digested with restriction endonucleases and examined in the electron microscope. Restriction fragments containing displacement loops (D-loop) were photographed and measured to determine the position of replicated sequences in relati...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.3843
更新日期：1984-05-11 00:00:00
abstract：:The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were u...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.2.1243
更新日期：1984-01-25 00:00:00
abstract：:Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.4.e12
更新日期：2000-02-15 00:00:00
abstract：:The EMBL Data Library was the first internationally supported central resource for nucleic acid sequence data. Working in close collaboration with its American counterpart, GenBank (1), the library prepares and makes available to the scientific community a comprehensive collection of the published nucleic acid sequenc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.1.5
更新日期：1986-01-10 00:00:00
abstract：:Identification of MHC-binding peptides is a prerequisite in rational design of T-cell based peptide vaccines. During the past decade a number of computational approaches have been introduced for the prediction of MHC-binding peptides, efficiently reducing the number of candidate binders that need to be experimentally ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl284
更新日期：2006-07-01 00:00:00
abstract：:The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. Th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.12.4597
更新日期：1985-06-25 00:00:00
abstract：:DNA supercoiling is an important regulator of gene activity. The transmission of transcription-generated supercoiling wave along a DNA helix provides a way for a gene being transcribed to communicate with and regulate its neighboring genes. Currently, the dynamic behavior of supercoiling transmission remains unclear o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky534
更新日期：2018-08-21 00:00:00
abstract：:The identification and characterization of binding sites of DNA-binding molecules, including transcription factors (TFs), is a critical problem at the interface of chemistry, biology and molecular medicine. The Cognate Site Identification (CSI) array is a high-throughput microarray platform for measuring comprehensive...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn057
更新日期：2008-06-01 00:00:00
abstract：:The DNA damage checkpoint halts cell cycle progression in G2 in response to genotoxic insults. Central to the execution of cell cycle arrest is the checkpoint-induced stabilization of securin-separase complex (yeast Pds1-Esp1). The checkpoint kinases Chk1 and Chk2 (yeast Chk1 and Rad53) are thought to critically contr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa355
更新日期：2020-06-19 00:00:00
abstract：:The results of differential expression analyses provide scientists with hundreds to thousands of differentially expressed genes that need to be interpreted in light of the biology of the specific system under study. This requires mapping the genes to functional classifications that can be, for example, the KEGG pathwa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt516
更新日期：2013-07-01 00:00:00
abstract：:We have examined the behaviour of the beta-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the beta-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the beta-interferon p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.21.4489
更新日期：1994-10-25 00:00:00
abstract：:A lac promotor mutant was constructed by filling in the protruding ends of the HpaII site located within the lac promotor. The mutation, named M42, is a two base pair insertion that changes the distance between the -10 and -35 regions from 18 to 20 residues. The activity of the mutant promotor measured in vivo is 15% ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.3.903
更新日期：1982-02-11 00:00:00
abstract：:DNA-binding and RNA-binding proteins are usually considered 'undruggable' partly due to the lack of an efficient method to identify inhibitors from existing small molecule repositories. Here we report a rapid and sensitive high-throughput screening approach to identify compounds targeting protein-nucleic acids interac...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv069
更新日期：2015-04-30 00:00:00
abstract：:Programmed -1 ribosomal frameshifting is employed in the expression of a number of viral and cellular genes. In this process, the ribosome slips backwards by a single nucleotide and continues translation of an overlapping reading frame, generating a fusion protein. Frameshifting signals comprise a heptanucleotide slip...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks629
更新日期：2012-09-01 00:00:00
abstract：:TERC is an RNA component of telomerase. However, TERC is also ubiquitously expressed in most human terminally differentiated cells, which don't have telomerase activity. The function of TERC in these cells is largely unknown. Here, we report that TERC enhances the expression and secretion of inflammatory cytokines by ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz584
更新日期：2019-09-05 00:00:00
abstract：:Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq769
更新日期：2011-01-01 00:00:00
abstract：:Fast viral adaptation and the implication of this rapid evolution in the emergence of several new infectious diseases have turned this issue into a major challenge for various research domains. Indeed, viruses are involved in the development of a wide range of pathologies and understanding how viruses and host cells i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1013
更新日期：2011-01-01 00:00:00
abstract：:The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.21.7607
更新日期：1985-11-11 00:00:00
abstract：:Advances in genomic sequencing have enormous potential to revolutionize personalized medicine, however distinguishing disease-causing from benign variants remains a challenge. The increasing number of human genome and exome sequences available has revealed areas where unfavourable variation is removed through purifyin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz457
更新日期：2019-07-02 00:00:00
abstract：:The Phospho.ELM resource (http://phospho.elm.eu.org) is a relational database designed to store in vivo and in vitro phosphorylation data extracted from the scientific literature and phosphoproteomic analyses. The resource has been actively developed for more than 7 years and currently comprises 42,574 serine, threoni...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1104
更新日期：2011-01-01 00:00:00
abstract：:Molecular replacement (MR) is the method of choice for X-ray crystallography structure determination when structural homologues are available in the Protein Data Bank (PDB). Although the success rate of MR decreases sharply when the sequence similarity between template and target proteins drops below 35% identical res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh400
更新日期：2004-07-01 00:00:00
abstract：:Sox proteins belong to the HMG box superfamily of DNA-binding proteins and are found throughout the animal kingdom. They are involved in the regulation of such diverse developmental processes as germ layer formation, organ development and cell type specifi-cation. Hence, deletion or mutation of Sox proteins often resu...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/27.6.1409
更新日期：1999-03-15 00:00:00
abstract：:The DNA sequence was determined for the narL gene of Escherichia coli. This gene is involved in global regulation of a number of nitrate controlled genes including frdABCD, tor, narGHJI, and adhE which are associated with bacterial respiration and fermentation. Comparison of the deduced amino acid sequence of narL to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.5.1965
更新日期：1989-03-11 00:00:00
abstract：:The promoter of the human BCR gene, regulating the transcription of the chimeric BCR/ABL mRNA in leukemia, has been isolated and characterized. A region of 1.1 kb immediately 5' to the transcription start site was analyzed in detail by sequencing, DNase 1 footprinting, gel retardation and functional studies. These exp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.23.7119
更新日期：1990-12-11 00:00:00
abstract：:The Mouse Genome Database (MGD, http://www.informatics.jax.org/), integrates genetic, genomic and phenotypic information about the laboratory mouse, a primary animal model for studying human biology and disease. Information in MGD is obtained from diverse sources, including the scientific literature and external datab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn886
更新日期：2009-01-01 00:00:00
abstract：:Knowledge of subcellular localizations (SCLs) of plant proteins relates to their functions and aids in understanding the regulation of biological processes at the cellular level. We present PlantLoc, a highly accurate and fast webserver for predicting the multi-label SCLs of plant proteins. The PlantLoc server has two...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt428
更新日期：2013-07-01 00:00:00
abstract：:p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1075
更新日期：2007-01-01 00:00:00
abstract：:The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.14.3887
更新日期：1991-07-25 00:00:00
