Abstract:
:DNA supercoiling is an important regulator of gene activity. The transmission of transcription-generated supercoiling wave along a DNA helix provides a way for a gene being transcribed to communicate with and regulate its neighboring genes. Currently, the dynamic behavior of supercoiling transmission remains unclear owing to the lack of a suitable tool for detecting the dynamics of supercoiling transmission. In this work, we established a torsion sensor that quantitatively monitors supercoiling transmission in real time in DNA. Using this sensor, we studied the transmission of transcriptionally generated negative supercoiling in linear and multi-way DNA duplexes. We found that transcription-generated dynamic supercoiling not only transmits along linear DNA duplex but also equally diverges at and proceeds through multi-way DNA junctions. We also show that such a process is regulated by DNA-protein interactions and non-canonical DNA structures in the path of supercoiling transmission. These results imply a transcription-coupled mechanism of dynamic supercoiling-mediated intra- and inter-chromosomal signal transduction pathway and their regulation in DNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Xia Y,Zheng KW,He YD,Liu HH,Wen CJ,Hao YH,Tan Zdoi
10.1093/nar/gky534subject
Has Abstractpub_date
2018-08-21 00:00:00pages
7418-7424issue
14eissn
0305-1048issn
1362-4962pii
5047275journal_volume
46pub_type
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