High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.

abstract：:Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, is able to catenate in vitro closed DNA circles in a magnesium-dependent, ATP-dependent, histone H1-dependent reaction, and to decatenate in vitro kinetoplast DNA n...

journal_title：Nucleic acids research

authors： Duguet M,Lavenot C,Harper F,Mirambeau G,De Recondo AM

更新日期：1983-02-25 00:00:00

abstract：:We have investigated the distribution of mitochondrial DNA polymorphisms in a rare maternally transmitted genetic trait that causes hypersensitivity to aminoglycoside antibiotics, in the hope that a characterization of its molecular basis might provide a molecular and cellular understanding of aminoglycoside-induced d...

journal_title：Nucleic acids research

authors： Hutchin T,Haworth I,Higashi K,Fischel-Ghodsian N,Stoneking M,Saha N,Arnos C,Cortopassi G

更新日期：1993-09-11 00:00:00

abstract：:Telomerase is a ribonucleoprotein reverse transcriptase that synthesizes and maintains telomeric DNA. Studies of telomeres and telomerase are facilitated by the large number of linear DNA molecules found in ciliated protozoa, such as Tetrahymena thermophila. To examine the expression of telomerase, we investigated the...

journal_title：Nucleic acids research

authors： Hargrove BW,Bhattacharyya A,Domitrovich AM,Kapler GM,Kirk K,Shippen DE,Kunkel GR

更新日期：1999-11-01 00:00:00

abstract：:The use of bacterial artificial chromosomes (BACs) provides a consistent and high targeting efficiency of homologous recombination in embryonic stem (ES) cells, facilitated by long stretches of sequence homology. Here, we introduce a BAC targeting method which employs restriction fragment length polymorphisms (RFLPs) ...

journal_title：Nucleic acids research

authors： Barakat TS,Rentmeester E,Sleutels F,Grootegoed JA,Gribnau J

更新日期：2011-10-01 00:00:00

abstract：:The Xist lncRNA requires Repeat A, a conserved RNA element located in its 5' end, to induce gene silencing during X-chromosome inactivation. Intriguingly, Repeat A is also required for production of Xist. While silencing by Repeat A requires the protein SPEN, how Repeat A promotes Xist production remains unclear. We r...

journal_title：Nucleic acids research

authors： Trotman JB,Lee DM,Cherney RE,Kim SO,Inoue K,Schertzer MD,Bischoff SR,Cowley DO,Calabrese JM

更新日期：2020-10-09 00:00:00

abstract：:Using cyanoethyldiisopropylamino phosphoramidite chemistry, four oligonucleotides constituting a part of the sequence of the initiator t-RNA from B. subtilis were synthesized. For the protection of the exocyclic amino functions of bases, phenoxyacetyl group was used for adenine and guanine, and acetyl group was prefer...

journal_title：Nucleic acids research

authors： Chaix C,Duplaa AM,Molko D,Téoule R

更新日期：1989-09-25 00:00:00

abstract：:Transcriptomics (at the level of single cells, tissues and/or whole organisms) underpins many fields of biomedical science, from understanding the basic cellular function in model organisms, to the elucidation of the biological events that govern the development and progression of human diseases, and the exploration o...

journal_title：Nucleic acids research

authors： Cantacessi C,Jex AR,Hall RS,Young ND,Campbell BE,Joachim A,Nolan MJ,Abubucker S,Sternberg PW,Ranganathan S,Mitreva M,Gasser RB

更新日期：2010-09-01 00:00:00

abstract：:The Homeodomain Resource is a comprehensive collection of sequence, structure and genomic information on the homeodomain protein family. Available through the Resource are both full-length and domain-only sequence data, as well as X-ray and NMR structural data for proteins and protein-DNA complexes. Also available is ...

journal_title：Nucleic acids research

authors： Banerjee-Basu S,Ferlanti ES,Ryan JF,Baxevanis AD

更新日期：1999-01-01 00:00:00

abstract：:Findings from clinical and biological studies are often not reproducible when tested in independent cohorts. Due to the testing of a large number of hypotheses and relatively small sample sizes, results from whole-genome expression studies in particular are often not reproducible. Compared to single-study analysis, ge...

journal_title：Nucleic acids research

authors： Sweeney TE,Haynes WA,Vallania F,Ioannidis JP,Khatri P

更新日期：2017-01-09 00:00:00

abstract：:Most mutations in cancer genomes occur in the non-coding regions with unknown impact on tumor development. Although the increase in the number of cancer whole-genome sequences has revealed numerous putative non-coding cancer drivers, their information is dispersed across multiple studies making it difficult to underst...

journal_title：Nucleic acids research

authors： Liu EM,Martinez-Fundichely A,Bollapragada R,Spiewack M,Khurana E

更新日期：2021-01-08 00:00:00

abstract：:ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicy...

journal_title：Nucleic acids research

authors： Kumarevel T,Tanaka T,Umehara T,Yokoyama S

更新日期：2009-08-01 00:00:00

abstract：:LINE-1 (L1) is a highly successful autonomous non-LTR retrotransposon and a major force shaping mammalian genomes. Although there are about 600 000 L1 copies covering 23% of the rat genome, full-length rat L1s (L1Rn) with intact open reading frames (ORFs) representing functional master copies for retrotransposition ha...

journal_title：Nucleic acids research

authors： Kirilyuk A,Tolstonog GV,Damert A,Held U,Hahn S,Löwer R,Buschmann C,Horn AV,Traub P,Schumann GG

更新日期：2008-02-01 00:00:00

abstract：:Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle. Here, we demonstrated that the telomerase-recruitment domain of Cdc13 is also phosphorylated by Cdk1 at the same cell cycle stage as the Tel1/Mec1-dependent regulation. Phosphor-specific gel an...

journal_title：Nucleic acids research

authors： Tseng SF,Shen ZJ,Tsai HJ,Lin YH,Teng SC

更新日期：2009-06-01 00:00:00

abstract：:A coiled-coil microtubule-bundling protein, p180, was originally identified as one of the ribosome receptor candidates on the rough endoplasmic reticulum (ER) and is highly expressed in secretory tissues. Recently, we reported that p180 plays crucial roles in upregulating collagen biosynthesis, mainly by facilitating ...

journal_title：Nucleic acids research

authors： Ueno T,Kaneko K,Sata T,Hattori S,Ogawa-Goto K

更新日期：2012-04-01 00:00:00

abstract：:RNA-protein complexes are essential in mediating important fundamental cellular processes, such as transport and localization. In particular, ncRNA-protein interactions play an important role in post-transcriptional gene regulation like mRNA localization, mRNA stabilization, poly-adenylation, splicing and translation....

journal_title：Nucleic acids research

authors： Suresh V,Liu L,Adjeroh D,Zhou X

更新日期：2015-02-18 00:00:00

abstract：:Recently, prokaryotic riboswitches have been identified that regulate transcription in response to change of the concentration of secondary messengers. The ZMP (5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR))-sensing riboswitch from Thermosinus carboxydivorans is a transcriptional ON-switch that is involved in ...

journal_title：Nucleic acids research

authors： Binas O,Schamber T,Schwalbe H

更新日期：2020-07-09 00:00:00

abstract：:A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned...

journal_title：Nucleic acids research

authors： Schollmeier K,Gärtner D,Hillen W

更新日期：1985-06-25 00:00:00

abstract：:A new method of representing DNA sequences has been devised which is termed stave projection. Compared with other formats for showing the base sequences of DNA, this method greatly enhances the ease of visual analysis of the sequences of bases and it is also in a machine readable form. Using this method it is possible...

journal_title：Nucleic acids research

authors： Cowin JE,Jellis CH,Rickwood D

更新日期：1986-01-10 00:00:00

abstract：:BRENDA is a comprehensive relational database on functional and molecular information of enzymes, based on primary literature. The database contains information extracted and evaluated from approximately 46 000 references, holding data of at least 40 000 different enzymes from more than 6900 different organisms, class...

journal_title：Nucleic acids research

authors： Schomburg I,Chang A,Schomburg D

更新日期：2002-01-01 00:00:00

abstract：:The locus DXS269 (P20) defines a deletion hotspot in the distal part of the Duchenne Muscular Dystrophy gene. We have cloned over 90 kilobase-pairs of genomic DNA from this region in overlapping cosmids. The use of whole cosmids as probes in a competitive DNA hybridization analysis proves a fast and convenient method ...

journal_title：Nucleic acids research

authors： Blonden LA,den Dunnen JT,van Paassen HM,Wapenaar MC,Grootscholten PM,Ginjaar HB,Bakker E,Pearson PL,van Ommen GJ

更新日期：1989-07-25 00:00:00

abstract：:Metagenomics is a relatively recently established but rapidly expanding field that uses high-throughput next-generation sequencing technologies to characterize the microbial communities inhabiting different ecosystems (including oceans, lakes, soil, tundra, plants and body sites). Metagenomics brings with it a number ...

journal_title：Nucleic acids research

authors： Hunter S,Corbett M,Denise H,Fraser M,Gonzalez-Beltran A,Hunter C,Jones P,Leinonen R,McAnulla C,Maguire E,Maslen J,Mitchell A,Nuka G,Oisel A,Pesseat S,Radhakrishnan R,Rocca-Serra P,Scheremetjew M,Sterk P,Vaughan D,

更新日期：2014-01-01 00:00:00

abstract：:Lambda exonuclease processively degrades one strand of double-stranded DNA (dsDNA) in the 5"-3" direction. To understand the mechanism through which this enzyme generates high processivity we are analyzing the first step in the reaction, namely the interaction of lambda exonuclease with the ends of substrate DNA. Endo...

journal_title：Nucleic acids research

authors： Mitsis PG,Kwagh JG

更新日期：1999-08-01 00:00:00

abstract：:The RES complex was previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. This complex was shown to contain three subunits, namely Snu17, Bud13 and Pml1, but its mode of action remains ill-defined. To obtain insights into its function, we have performed a structural investigation of...

journal_title：Nucleic acids research

authors： Brooks MA,Dziembowski A,Quevillon-Cheruel S,Henriot V,Faux C,van Tilbeurgh H,Séraphin B

更新日期：2009-01-01 00:00:00

abstract：:The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly...

journal_title：Nucleic acids research

authors： Van Kreijl CF,Beelen RH,Borst P

更新日期：1977-02-01 00:00:00

abstract：:Double-stranded oligonucleotides (ODNs) containing the consensus binding sequence of a transcription factor provide a rationally designed tool to manipulate gene expression at the transcriptional level by the decoy approach. However, modifications introduced into oligonucleotides to increase stability quite often do n...

journal_title：Nucleic acids research

authors： Crinelli R,Bianchi M,Gentilini L,Palma L,Sørensen MD,Bryld T,Babu RB,Arar K,Wengel J,Magnani M

更新日期：2004-03-29 00:00:00

abstract：:Human Silent Information Regulator Type 1 (SIRT1) is an NAD(+)-dependent deacetylase protein which is an intermediary of cellular metabolism in gene silencing and aging. SIRT1 has been extensively investigated and shown to delay senescence; however, less is known about the regulation of SIRT1 during aging. In this stu...

journal_title：Nucleic acids research

authors： Han L,Zhou R,Niu J,McNutt MA,Wang P,Tong T

更新日期：2010-11-01 00:00:00

abstract：:Some human cancers maintain their telomeres using the alternative lengthening of telomeres (ALT) mechanism; a process thought to involve recombination. Different types of recombinational telomere elongation pathways have been identified in yeasts. In senescing yeast telomerase deletion (ter1-Delta) mutants with very s...

journal_title：Nucleic acids research

authors： Basenko EY,Cesare AJ,Iyer S,Griffith JD,McEachern MJ

更新日期：2010-01-01 00:00:00

abstract：:RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with argini...

journal_title：Nucleic acids research

authors： Rochat T,Bohn C,Morvan C,Le Lam TN,Razvi F,Pain A,Toffano-Nioche C,Ponien P,Jacq A,Jacquet E,Fey PD,Gautheret D,Bouloc P

更新日期：2018-09-28 00:00:00

abstract：:Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...

journal_title：Nucleic acids research

authors： Pavlov NA,Cherny DI,Nazimov IV,Slesarev AI,Subramaniam V

更新日期：2002-02-01 00:00:00

abstract：:The E. coli protein StpA has RNA annealing and strand displacement activities and it promotes folding of RNAs by loosening their structures. To understand the mode of action of StpA, we analysed the relationship of its RNA chaperone activity to its RNA-binding properties. For acceleration of annealing of two short RNA...

journal_title：Nucleic acids research

authors： Mayer O,Rajkowitsch L,Lorenz C,Konrat R,Schroeder R

更新日期：2007-01-01 00:00:00