Abstract:
:The 80S ribosome from Saccharomyces cerevisiae has been reconstructed from cryo electron micrographs to a resolution of 35 A. It is strikingly similar to the 70S ribosome from Escherichia coli, while displaying the characteristic eukaryotic features familiar from reconstructions of ribosomes from higher eukaryotes. Aside from the elaboration of a number of peripherally located features on the two subunits and greater overall size, the largest difference between the yeast and E.coli ribosomes is in a mass increase on one side of the large (60S) subunit. It thus appears more elliptical than the characteristically globular 50S subunit from E.coli. The interior of the 60S subunit reveals a variable diameter tunnel spanning the subunit between the interface canyon and a site on the lower back of the subunit, presumably the exit site through which the nascent polypeptide chain emerges from the ribosome.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Verschoor A,Warner JR,Srivastava S,Grassucci RA,Frank Jdoi
10.1093/nar/26.2.655subject
Has Abstractpub_date
1998-01-15 00:00:00pages
655-61issue
2eissn
0305-1048issn
1362-4962pii
gkb117journal_volume
26pub_type
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