The effect of sequence specific DNA methylation on restriction endonuclease cleavage.

abstract：:Alternative splicing constitutes a major mechanism creating protein diversity in humans. This diversity can result from the alternative skipping of entire exons or by alternative selection of the 5' or 3' splice sites that define the exon boundaries. In this study, we analyze the sequence and evolutionary characterist...

journal_title：Nucleic acids research

authors： Akerman M,Mandel-Gutfreund Y

更新日期：2007-01-01 00:00:00

abstract：:The genes encoding the RNA subunit of ribonuclease P from the unicellular cyanobacterium Synechocystis sp. PCC 6803, and from the heterocyst-forming strains Anabaena sp. PCC 7120 and Calothrix sp. PCC 7601 were cloned using the homologous gene from Anacystis nidulans (Synechococcus sp. PCC 6301) as a probe. The genes ...

journal_title：Nucleic acids research

authors： Vioque A

更新日期：1992-12-11 00:00:00

abstract：:DNA polymerase mu is a member of the mammalian pol X family and reduces deletion during chromosome break repair by nonhomologous end joining (NHEJ). This biological role is linked to pol mu's ability to promote NHEJ of ends with noncomplementary 3' overhangs, but questions remain regarding how it performs this role. W...

journal_title：Nucleic acids research

authors： Davis BJ,Havener JM,Ramsden DA

更新日期：2008-05-01 00:00:00

abstract：:The control of messenger RNA (mRNA) function by micro RNAs (miRNAs) in animal cells requires the GW182 protein. GW182 is recruited to the miRNA repression complex via interaction with Argonaute protein, and functions downstream to repress protein synthesis. Interaction with Argonaute is mediated by GW/WG repeats, whic...

journal_title：Nucleic acids research

authors： Chekulaeva M,Parker R,Filipowicz W

更新日期：2010-10-01 00:00:00

abstract：:Modular primers are strings of three contiguously annealed unligated oligonucleotides (modules) as short as 5- or 6-mers, selected from a presynthesized library. It was previously found that such strings can prime DNA sequencing reactions specifically, thus eliminating the need for the primer synthesis step in DNA seq...

journal_title：Nucleic acids research

authors： Beskin AD,Zevin-Sonkin D,Sobolev IA,Ulanovsky LE

更新日期：1995-08-11 00:00:00

abstract：:DNA curvature plays a well-characterized role in many transcriptional regulation mechanisms. We present evidence for the conservation of curvature signals in putative regulatory regions of several archaeal and eubacterial genomes. Genes with highly curved upstream regions were identified in orthologous groups, based o...

journal_title：Nucleic acids research

authors： Jáuregui R,Abreu-Goodger C,Moreno-Hagelsieb G,Collado-Vides J,Merino E

更新日期：2003-12-01 00:00:00

abstract：:In eukaryotes, DNA is packaged into a basic unit, the nucleosome which consists of 147 bp of DNA wrapped around a histone octamer composed of two copies each of the histones H2A, H2B, H3 and H4. Nucleosome structures are diverse not only by histone variants, histone modifications, histone composition but also through ...

journal_title：Nucleic acids research

authors： Ngo TT,Ha T

更新日期：2015-04-30 00:00:00

abstract：:Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by optical mapping of their DNA. We use a novel, one-step chemo-enzymatic reaction to covalently bind fluorophores to DNA at the four-base recognition sites of a ...

journal_title：Nucleic acids research

authors： Grunwald A,Dahan M,Giesbertz A,Nilsson A,Nyberg LK,Weinhold E,Ambjörnsson T,Westerlund F,Ebenstein Y

更新日期：2015-10-15 00:00:00

abstract：:PAHdb (http://www.mcgill.ca/pahdb ) is a curated relational database (Fig. 1) of nucleotide variation in the human PAH cDNA (GenBank U49897). Among 328 different mutations by state (Fig. 2) the majority are rare mutations causing hyperphenylalaninemia (HPA) (OMIM 261600), the remainder are polymorphic variants without...

journal_title：Nucleic acids research

authors： Nowacki PM,Byck S,Prevost L,Scriver CR

更新日期：1998-01-01 00:00:00

abstract：:RegAnalyst is a user-friendly web interface that integrates MoPP (Motif Prediction Program), MyPatternFinder (pattern detection tool) and MycoRegDB (mycobacterial promoter and regulatory elements database). Since motif discovery is a challenging task, numerous tools have been developed over the past few years. Strikin...

journal_title：Nucleic acids research

authors： Sharma D,Mohanty D,Surolia A

更新日期：2009-07-01 00:00:00

abstract：:The Prp19-associated complex is required for spliceosome activation by stabilizing the binding of U5 and U6 on the spliceosome after the release of U4. The complex comprises at least eight proteins, among which Ntc90 and Ntc77 contain multiple tetratricopeptide repeat (TPR) elements. We have previously shown that Ntc9...

journal_title：Nucleic acids research

authors： Chen HC,Chang KJ,Su YL,Huang YH,Cheng SC

更新日期：2014-10-29 00:00:00

abstract：:Upon growth transition of Escherichia coli cells from exponential to stationary phase, RNA polymerase is converted into at least three different forms (S1, S2 and S3), which could be separately isolated by phosphocellulose column chromatography (Ozaki et al., 1991 (2)). Here, the promoter selectivity of these three st...

journal_title：Nucleic acids research

authors： Ozaki M,Fujita N,Wada A,Ishihama A

更新日期：1992-01-25 00:00:00

abstract：:The ability to store and interconnect all available information on proteins is crucial to modern biological research. Accordingly, the Universal Protein Resource (UniProt) plays an increasingly important role by providing a stable, comprehensive, freely accessible central resource on protein sequences and functional a...

journal_title：Nucleic acids research

authors： UniProt Consortium.

更新日期：2007-01-01 00:00:00

abstract：:The specific recognition of homopurine-homo pyrimidine regions in duplex DNA by triplex-forming oligonucleotides (TFOs) provides an attractive strategy for genetic manipulation. Alkylation of nucleobases with functionalized TFOs would have the potential for site-directed mutagenesis. Recently, we demonstrated that a T...

journal_title：Nucleic acids research

authors： Nagatsugi F,Sasaki S,Miller PS,Seidman MM

更新日期：2003-03-15 00:00:00

abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...

journal_title：Nucleic acids research

authors： McLaughlin CR,Tao Q,Abood ME

更新日期：1994-12-11 00:00:00

abstract：:The microbial genome database for comparative analysis (MBGD) (available at http://mbgd.genome.ad.jp/) is a comprehensive ortholog database for flexible comparative analysis of microbial genomes, where the users are allowed to create an ortholog table among any specified set of organisms. Because of the rapid increase...

journal_title：Nucleic acids research

authors： Uchiyama I,Mihara M,Nishide H,Chiba H

更新日期：2015-01-01 00:00:00

abstract：:2'-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNA cytosine methyltransferases methylate the 5-position of DNA cytosine residues in specific sequence contexts. Chemical s...

journal_title：Nucleic acids research

authors： Schmidt S,Pein CD,Fritz HJ,Cech D

更新日期：1992-05-25 00:00:00

abstract：:For sophisticated gene targeting procedures requiring two sequential selective steps to operate efficiently it is essential that the marker genes used are not prone to position effects. The double replacement gene targeting procedure, to produce mice with subtle gene alterations, is based on the use of hypoxanthine ph...

journal_title：Nucleic acids research

authors： Melton DW,Ketchen AM,Selfridge J

更新日期：1997-10-01 00:00:00

abstract：:We have investigated the substrate specificity of the Ogg1 protein of Saccharomyces cerevisiae (yOgg1 protein) for excision of modified DNA bases from oxidatively damaged DNA substrates using gas chromatography/isotope dilution mass spectrometry. Four DNA substrates prepared by treatment with H2O2/Fe(III)-EDTA/ascorbi...

journal_title：Nucleic acids research

authors： Karahalil B,Girard PM,Boiteux S,Dizdaroglu M

更新日期：1998-03-01 00:00:00

abstract：:The transcription factor CHOP/GADD153 gene is induced by cellular stress and is involved in mediating apoptosis. We report the identification of a conserved region in the promoter of the CHOP gene responsible for its inducibility by endoplasmic reticulum (ER) stress. Deletion mutants of the human CHOP promoter identif...

journal_title：Nucleic acids research

authors： Ubeda M,Habener JF

更新日期：2000-12-15 00:00:00

abstract：:Thymine DNA glycosylase (TDG) is a DNA repair enzyme that excises a variety of mismatched or damaged nucleotides (nts), e.g. dU, dT, 5fC and 5caC. TDG is shown to play essential roles in maintaining genome integrity and correctly programming epigenetic modifications through DNA demethylation. After locating the lesion...

journal_title：Nucleic acids research

authors： Da LT,Yu J

更新日期：2018-06-20 00:00:00

abstract：:Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome...

journal_title：Nucleic acids research

authors： Hefel A,Honda M,Cronin N,Harrell K,Patel P,Spies M,Smolikove S

更新日期：2021-01-21 00:00:00

abstract：:A truly universal nucleobase enables a host of novel applications such as simplified templates for PCR primers, randomized sequencing and DNA based devices. A universal base must pair indiscriminately to each of the canonical bases with little or preferably no destabilization of the overall duplex. In reality, many ca...

journal_title：Nucleic acids research

authors： Spring-Connell AM,Evich MG,Debelak H,Seela F,Germann MW

更新日期：2016-10-14 00:00:00

abstract：:Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive sequences had the same organization in micro- and macronuclear DNAs as revealed by blot hybridization. On the other hand, t...

journal_title：Nucleic acids research

authors： Iwamura Y,Sakai M,Muramatsu M

更新日期：1982-07-24 00:00:00

abstract：:The sarcomeric myosin gene, Myh7b, encodes an intronic microRNA, miR-499, which regulates cardiac and skeletal muscle biology, yet little is known about its transcriptional regulation. To identify the transcription factors involved in regulating Myh7b/miR-499 gene expression, we have mapped the transcriptional start s...

journal_title：Nucleic acids research

authors： Yeung F,Chung E,Guess MG,Bell ML,Leinwand LA

更新日期：2012-08-01 00:00:00

abstract：:The secondary structures of the 5'-untranslated region (5'-UTR) of five different tymoviruses have been determined by structure probing, computer prediction and sequence comparison. Despite large sequence differences, there are remarkable similarities in the secondary structure. In all viruses two or four hairpins are...

journal_title：Nucleic acids research

authors： Hellendoorn K,Michiels PJ,Buitenhuis R,Pleij CW

更新日期：1996-12-15 00:00:00

abstract：:ANISEED (www.aniseed.cnrs.fr) is the main model organism database for tunicates, the sister-group of vertebrates. This release gives access to annotated genomes, gene expression patterns, and anatomical descriptions for nine ascidian species. It provides increased integration with external molecular and taxonomy datab...

journal_title：Nucleic acids research

authors： Brozovic M,Dantec C,Dardaillon J,Dauga D,Faure E,Gineste M,Louis A,Naville M,Nitta KR,Piette J,Reeves W,Scornavacca C,Simion P,Vincentelli R,Bellec M,Aicha SB,Fagotto M,Guéroult-Bellone M,Haeussler M,Jacox E,Lowe

更新日期：2018-01-04 00:00:00

abstract：:Single nucleotide polymorphisms (SNPs) are, together with copy number variation, the primary source of variation in the human genome. SNPs are associated with altered response to drug treatment, susceptibility to disease and other phenotypic variation. Furthermore, during genetic screens for disease-associated mutatio...

journal_title：Nucleic acids research

authors： Reumers J,Conde L,Medina I,Maurer-Stroh S,Van Durme J,Dopazo J,Rousseau F,Schymkowitz J

更新日期：2008-01-01 00:00:00

abstract：:The Class 3 aldehyde dehydrogenase gene (ALDH-3) is differentially expressed. Expression is either constitutive or xenobiotic inducible via an aromatic hydrocarbon (Ah) receptor-mediated pathway, depending upon the tissue. A series of studies were performed to examine the regulation of rat ALDH-3 basal expression. DNa...

journal_title：Nucleic acids research

authors： Xie YQ,Takimoto K,Pitot HC,Miskimins WK,Lindahl R

更新日期：1996-11-01 00:00:00

abstract：:A cDNA that encodes a transcription factor able to recognize the thyroglobulin gene promoter in vitro was isolated from a dog thyroid cDNA expression library in lambda gt11. The library was screened with a multimerized 20 bp-oligonucleotide probe corresponding to the -126 to -107 bp region of the bovine thyroglobulin ...

journal_title：Nucleic acids research

authors： Javaux F,Donda A,Vassart G,Christophe D

更新日期：1991-03-11 00:00:00