Abstract：
:Chloroplast DNA, isolated from a synchronized culture of Chlamydomonas reinhardii, was digested with restriction endonucleases and examined in the electron microscope. Restriction fragments containing displacement loops (D-loop) were photographed and measured to determine the position of replicated sequences in relation to the restriction enzyme sites. D-loops were located at two positions on the physical map of chloroplast DNA. One replication origin was mapped at about 10 kb upstream of the 5' end of a 16s rRNA gene. The second origin was spaced 6. 5kb apart from the first origin and was about 16.5 kb upstream of the same 16s rRNA. Initiations at those two sites were not always synchronized. Replication initiated with the formation of a D-loop resulting from the synthesis of one daughter strand. After a short initial lag phase, corresponding to the synthesis of 350 +/- 130 bp of one daughter strand, DNA synthesis then proceeded in both directions. Both D-loop regions were preferred binding sites of undetermined protein complexes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Waddell J,Wang XM,Wu Mdoi
10.1093/nar/12.9.3843subject
Has Abstractpub_date
1984-05-11 00:00:00pages
3843-56issue
9eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract：:Interactions between subunits in the Saccharomyces cerevisiae ribosome are mediated by universal and eukaryote-specific intersubunit bridges. Universal bridges are positioned close to the ribosomal functional centers, while eukaryote-specific bridges are mainly located on the periphery of the ribosome. Two bridges, eB...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1083
更新日期：2019-01-10 00:00:00
abstract：:Competition binding and UV melting studies of a DNA model system consisting of three, four or five mutually complementary oligonucleotides demonstrate that unpaired bases at the branch point stabilize three- and five-way junction loops but destabilize four-way junctions. The inclusion of unpaired nucleotides permits t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.12.2212
更新日期：1995-06-25 00:00:00
abstract：:The human mitochondrial translation apparatus, which synthesizes the core subunits of the oxidative phosphorylation system, is of central interest as mutations in several genes encoding for mitoribosomal proteins or translation factors cause severe human diseases. Little is known, how this complex machinery assembles ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky701
更新日期：2018-09-19 00:00:00
abstract：:Cellular RNAs that do not function as messenger RNAs (mRNAs), transfer RNAs (tRNAs) or ribosomal RNAs (rRNAs) comprise a diverse class of molecules that are commonly referred to as non-protein-coding RNAs (ncRNAs). These molecules have been known for quite a while, but their importance was not fully appreciated until ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkj469
更新日期：2006-01-25 00:00:00
abstract：:Thermally induced structural transition in the d(TTTTATAATAAA) d(TTTATTATAAAA) heteroduplex is characterized by UV-spectroscopy and differential scanning calorimetry. At low salt (less than 0.1 M) the occurrence of a cooperative transition in the lower temperature range, followed by a broad transition connected with s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.7.3245
更新日期：1984-04-11 00:00:00
abstract：:The Génolevures online database (http://cbi.labri.fr/Genolevures/ and http://genolevures.org/) provides exploratory tools and curated data sets relative to nine complete and seven partial genome sequences determined and manually annotated by the Génolevures Consortium, to facilitate comparative genomic studies of Hemi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn859
更新日期：2009-01-01 00:00:00
abstract：:RNase P RNA (RPR), the catalytic subunit of the essential RNase P ribonucleoprotein, removes the 5' leader from precursor tRNAs. The ancestral eukaryotic RPR is a Pol III transcript generated with mature termini. In the branch of the arthropod lineage that led to the insects and crustaceans, however, a new allele aros...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz572
更新日期：2019-09-19 00:00:00
abstract：:Sequence heterogeneity is a common characteristic of RNA viruses that is often referred to as sub-populations or quasispecies. Traditional techniques used for assembly of short sequence reads produced by deep sequencing, such as de-novo assemblers, ignore the underlying diversity. Here, we introduce a novel algorithm ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx755
更新日期：2017-11-02 00:00:00
abstract：:Protein-RNA interactions play essential roles in a number of regulatory mechanisms for gene expression such as RNA splicing, transport, translation and post-transcriptional control. As the number of available protein-RNA complex 3D structures has increased, it is now possible to statistically examine protein-RNA inter...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl819
更新日期：2006-01-01 00:00:00
abstract：:IMGT, the international ImMunoGeneTics database (http://imgt.cines. fr:8104 ), is a high-quality integrated database specialising in Immunoglobulins (Ig), T cell Receptors (TcR) and Major Histocompatibility Complex (MHC) molecules of all vertebrate species, created in 1989 by Marie-Paule Lefranc, Université Montpellie...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.219
更新日期：2000-01-01 00:00:00
abstract：:Mature chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength are enriched in beta-globin DNA sequences. Vitellogenin chromatin, which is not expressed in this tissue, is found in aggregation prone, salt insoluble chromatin. There is a direct correlation between the size of soluble fragm...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.3.1081
更新日期：1987-02-11 00:00:00
abstract：:RNA structural complexity and flexibility present a challenge for computational modeling efforts. Experimental information and bioinformatics data can be used as restraints to improve the accuracy of RNA tertiary structure prediction. Regarding utilization of restraints, the fundamental questions are: (i) What is the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz427
更新日期：2019-06-20 00:00:00
abstract：:Arginyl-tRNA synthetase (ArgRS) catalyses AMP- and PPi-independent deacylation of Arg-tRNAArg in the presence of cysteine. A dipeptide, Arg-Cys, is a product of this deacylation reaction. Similar reaction with homocysteine yields Arg-Hcy. Arginine is a noncompetitive inhibitor of the cysteine-dependent deacylation whi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.22.4608
更新日期：1995-11-25 00:00:00
abstract：:A cDNA (2855 nt) encoding a putative cytotoxic ribonuclease (rapLR1) related to the antitumor protein onconase was cloned from a library derived from the liver of gravid female amphibian Rana pipiens. The cDNA was mainly comprised (83%) of 3' untranslated region (UTR). Secondary structure analysis predicted two unusua...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.12.2375
更新日期：2000-06-15 00:00:00
abstract：:HIV-1 reverse transcriptase (RT) is a primary target for anti-AIDS drugs. Structures of HIV-1 RT, usually determined at approximately 2.5-3.0 A resolution, are important for understanding enzyme function and mechanisms of drug resistance in addition to being helpful in the design of RT inhibitors. Despite hundreds of ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn464
更新日期：2008-09-01 00:00:00
abstract：:The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.10.1758
更新日期：1995-05-25 00:00:00
abstract：:Unmodified tRNA molecules are useful for many purposes in cell-free protein biosynthesis, but there is little information about how the lack of tRNA post-transcriptional modifications affects the coding specificity for synonymous codons. In the present study, we prepared an unmodified form of Escherichia coli tRNA1Ser...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.15.2894
更新日期：1996-08-01 00:00:00
abstract：:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.11.1549
更新日期：1974-11-01 00:00:00
abstract：:Signal recognition particle (SRP) is a stable cytoplasmic ribonucleoprotein complex that serves to translocate secretory proteins across membranes during translation. The SRP Database (SRPDB) provides compilations of SRP components, ordered alphabetically and phylogenetically. Alignments emphasize phylogenetically-sup...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.1.169
更新日期：2001-01-01 00:00:00
abstract：:We have characterized elongation complexes (ECs) of RNA polymerase from the extremely thermophilic bacterium, Thermus thermophilus. We found that complexes assembled on nucleic acid scaffolds are transcriptionally competent at high temperature (50-80 degrees C) and, depending upon the organization of the scaffold, pos...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl559
更新日期：2006-01-01 00:00:00
abstract：:Although Dicer is essential for general microRNA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent. Instead, its short pre-miRNA hairpin is 'sliced' by Ago2, then 3'-resected into mature miRNAs. Here, we show that Drosophila cells and animals generate functional small RNAs from mir-451-type precursors. Howev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1038
更新日期：2014-02-01 00:00:00
abstract：:Bacteria belonging to the class Mollicutes were among the first ones to be selected for complete genome sequencing because of the minimal size of their genomes and their pathogenicity for humans and a broad range of animals and plants. At this time six genome sequences have been publicly released (Mycoplasma genitaliu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh114
更新日期：2004-01-01 00:00:00
abstract：:The DNA double helix undergoes an 'overstretching' transition in a narrow force range near 65 pN. Despite numerous studies the basic question of whether the strands are separated or not remains controversial. Here we show that overstretching in fact involves two distinct types of double-helix reorganization: slow hyst...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq309
更新日期：2010-09-01 00:00:00
abstract：:Cooperative hybridization has been used to establish physical linkage between two loci on a DNA strand. Linkage was detected by hybridization to a new type of high-density oligonucleotide array. Each synthesis location on the array contains a mixture of two different probe sequences. Each of the two probes can hybridi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.6.1485
更新日期：1999-03-15 00:00:00
abstract：:Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.24.11737
更新日期：1988-12-23 00:00:00
abstract：:Alternative splicing (AS) is a post-transcriptional process considered to be responsible for the huge diversity of proteins in higher eukaryotes. AS events are regulated by different splicing factors (SFs) that bind to sequence elements on the RNA. SFmap is a web server for predicting putative SF binding sites in geno...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq444
更新日期：2010-07-01 00:00:00
abstract：:The homeobox-containing gene Hex is expressed in several cell types, including thyroid follicular cells, in which it regulates the transcription of tissue- specific genes. In this study the regulation of Hex promoter activity was investigated. Using co- transfection experiments, we demonstrated that the transcriptiona...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg295
更新日期：2003-04-01 00:00:00
abstract：:Arylsulfatase, produced by Chlamydomonas reinhardtii during sulfur-limited growth, is secreted into the periplasmic space and is readily assayed using a chromogenic substrate. To assess the usefulness of the gene encoding arylsulfatase (ars) as a reporter gene in C. reinhardtii, we have fused the promoter region of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.12.2959
更新日期：1992-06-25 00:00:00
abstract：:RNA sequencing has become an important method to perform hypothesis-free characterization of global gene expression. One of the limitations of RNA sequencing is that most sequence reads represent highly expressed transcripts, whereas low level transcripts are challenging to detect. To combine the benefits of tradition...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks816
更新日期：2013-01-07 00:00:00
abstract：:Investigation of biological processes using selective chemical interventions is generally applied in biomedical research and drug discovery. Many studies of this kind make use of gene expression experiments to explore cellular responses to chemical interventions. Recently, some research groups constructed libraries of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn610
更新日期：2008-11-01 00:00:00
