Abstract:
:Mature chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength are enriched in beta-globin DNA sequences. Vitellogenin chromatin, which is not expressed in this tissue, is found in aggregation prone, salt insoluble chromatin. There is a direct correlation between the size of soluble fragments and the degree of globin gene enrichment, with the largest fragments being most highly enriched. The highly globin enriched (about 50 fold) polynucleosomes contain significantly elevated levels of acetylated histones H4, H2A.Z, and H2B, and ubiquitinated (prefix "u") histones H2A and H2B (with a significant relative increase of uH2B over uH2A). These polynucleosomes were complexed with histones H1 and H5 but at a lower level than that found in unfractionated chromatin.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ridsdale JA,Davie JRdoi
10.1093/nar/15.3.1081subject
Has Abstractpub_date
1987-02-11 00:00:00pages
1081-96issue
3eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract::We have identified and sequenced two members of a chicken middle repetitive DNA sequence family. By reassociation kinetics, members of this family (termed CRl) are estimated to be present in 1500-7000 copies per chicken haploid genome. The first family member sequenced (CRlUla) is located approximately 2 kb upstream f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.20.5383
更新日期:1981-10-24 00:00:00
abstract::The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most prominent nuclear organelle. Each protein entry is annotated with information about its corresponding gene, its domain st...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj004
更新日期:2006-01-01 00:00:00
abstract::Nucleic acid double helices are stabilized by hydrogen bonding and stacking forces (a combination of hydrophobic, dispersive and electrostatic forces) of the base pairs in the helix. One would predict the hydrogen bonding contributions to increase and the stacking contributions to decrease as the water activity in the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm210
更新日期:2007-01-01 00:00:00
abstract::The higher order structure of the functionally important 530 loop in Escherichia coli 16S rRNA was studied in mutants with single base changes at position 517, which significantly impair translational fidelity. The 530 loop has been proposed to interact with the EF-Tu-GTP-aatRNA ternary complex during decoding. The re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.17.3563
更新日期:1995-09-11 00:00:00
abstract::We report the extended database and data mining resources newly released in the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). H-InvDB is a comprehensive annotation resource of human genes and transcripts, and consists of two main views and six sub-databases. The latest release of H-InvDB (release 6...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1020
更新日期:2010-01-01 00:00:00
abstract::The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. Th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.12.4597
更新日期:1985-06-25 00:00:00
abstract::The R-Coffee web server produces highly accurate multiple alignments of noncoding RNA (ncRNA) sequences, taking into account predicted secondary structures. R-Coffee uses a novel algorithm recently incorporated in the T-Coffee package. R-Coffee works along the same lines as T-Coffee: it uses pairwise or multiple seque...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn278
更新日期:2008-07-01 00:00:00
abstract::Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE elements were hybr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.21.4427
更新日期:1997-11-01 00:00:00
abstract::The DNA-binding specificity and affinity of the dimeric human transcription factor (TF) STAT1, were assessed by total internal reflectance fluorescence protein-binding microarrays (TIRF-PBM) to evaluate the effects of protein phosphorylation, higher-order polymerization and small-molecule inhibition. Active, phosphory...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1085
更新日期:2013-01-01 00:00:00
abstract::DNA sequences, that control expression of the spliced leader (SL) RNA gene in the parasitic protozoan Leishmania amazonensis, were mapped by block substitution mutagenesis. In the absence of a functional in vitro system for transcription, no promoter elements have yet been identified in this organism. We therefore dev...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.11.1959
更新日期:1994-06-11 00:00:00
abstract::We have previously shown that Y box-binding protein-1 (YB-1) binds preferentially to cisplatin-modified Y box sequences. Based on structural and biochemical data, we predicted that this protein binds single-stranded nucleic acids. In the present study we confirmed the prediction and also discovered some unexpected fun...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.5.1200
更新日期:2001-03-01 00:00:00
abstract::In flowering plants, RNA editing is a posttranscriptional process that converts specific C to U in organelle mRNAs. Nicotiana tabacum is an allotetraploid species derived from the progenitors of Nicotiana sylvestris and Nicotiana tomentosiformis. These Nicotiana species have been used as a model for understanding the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn629
更新日期:2008-11-01 00:00:00
abstract::Guanine-rich DNA sequences can form G-quadruplexes stabilized by stacked G-G-G-G tetrads in monovalent cation-containing solution. The length and number of individual G-tracts and the length and sequence context of linker residues define the diverse topologies adopted by G-quadruplexes. The review highlights recent so...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkm711
更新日期:2007-01-01 00:00:00
abstract::Hepatitis C virus (HCV) RNA is recognized and cleaved in vitro by RNase P enzyme near the AUG start codon. Because RNase P identifies transfer RNA (tRNA) precursors, it has been proposed that HCV RNA adopts structural similarities to tRNA. Here, we present experimental evidence of RNase P sensitivity conservation in n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki290
更新日期:2005-03-08 00:00:00
abstract::Ribosome biogenesis (RiBi) is an extremely energy intensive process that is critical for gene expression. It is thus highly regulated, including through the tightly coordinated expression of over 200 RiBi genes by positive and negative transcriptional regulators. We investigated RiBi regulation as cells initiated meio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz231
更新日期:2019-06-04 00:00:00
abstract::5' mediated cytoplasmic RNA decay is a conserved cellular process in eukaryotes. While the functions of the structured core domains in this pathway are well-studied, the role of abundant intrinsically disordered regions (IDRs) is lacking. Here we reconstitute the Dcp1:Dcp2 complex containing a portion of the disordere...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky233
更新日期:2018-07-06 00:00:00
abstract::The possible role of LG-1, a Tetrahymena specific HMG protein found in the macronuclear chromatin (Hamana, K. and Iwai, K. (1979) J. Biochem. 86, 789-794), was examined in relation to the chromatin structure. The chromatin isolated from cells synchronized at different stages of the cell cycle contained about one molec...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.22.5275
更新日期:1980-11-25 00:00:00
abstract::Three computer programs are described which may be used to translate a DNA sequence into a protein sequence, back translate the protein sequence into an ambiguous DNA sequence, and then do pattern searching in the ambiguous sequence. The programs are written in the C programming language, have been compiled to run on ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part2.819
更新日期:1984-01-11 00:00:00
abstract::Starting from a crude E. coli extract, two activities which specifically protect highly repetitive bacterial DNA sequences (called PU for Palindromic Unit or REP for Repetitive Extragenic Palindromic sequence) against a digestion with Exonuclease III have been purified. We show that one of these activities is due to t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.13.3941
更新日期:1990-07-11 00:00:00
abstract::As an increasing number of reliable protein-protein interactions (PPIs) become available and high-throughput experimental methods provide systematic identification of PPIs, there is a growing need for fast and accurate methods for discovering homologous PPIs of a newly determined PPI. PPISearch is a web server that ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp309
更新日期:2009-07-01 00:00:00
abstract::Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1293
更新日期:2021-01-21 00:00:00
abstract::It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-le...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.18.3638
更新日期:1995-09-25 00:00:00
abstract::The translational regulation of ferritin expression currently represents the only well characterized example for eukaryotic translational control by high affinity interactions between a specific cytoplasmic protein, iron regulatory factor [IRF], and an mRNA-binding site, the iron-responsive element [IRE], located in t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.21.5555
更新日期:1992-11-11 00:00:00
abstract::RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.23.7763
更新日期:1982-12-11 00:00:00
abstract::Molecular Biology Database List (MBDL) includes brief descriptions and pointers to Web sites for the various databases described in this issue as well as other Web sites presenting data sets relevant to molecular biology. This information is compiled into a list (http://www.oup.co.uk/nar/Volume_27/Issue_01/summary/ gk...
journal_title:Nucleic acids research
pub_type:
doi:10.1093/nar/27.1.1
更新日期:1999-01-01 00:00:00
abstract::A method for the facile preparation of oligoribonucleotide analogues containing beta-d-allofuranosyl nucleosides with additional functional groups tethered to the 6'-O positions is presented. It is based on the synthesis of two protected nucleosides carrying a 6'-O -bromopentyl and a 6'-O -methylaminopentyl substituen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.19.4315
更新日期:1998-10-01 00:00:00
abstract::Gene rewiring is a common evolutionary phenomenon in nature that may lead to extinction for living organisms. Recent studies on synthetic biology demonstrate that cells can survive genetic rewiring. This survival (adaptation) is often linked to the stochastic expression of rewired genes with random transcriptional cha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1366
更新日期:2015-01-01 00:00:00
abstract::Oriented DNA films prepared by the wet-spinning technique have been complexed with several ligands: the anthracycline antibiotic violamycin BI, the dipeptide L-carnosine, and the oligopeptide antibiotic netropsin. The formation of the DNA-ligand complexes is accompanied by dramatic changes of the conformational flexib...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.23.9165
更新日期:1984-12-11 00:00:00
abstract::A U3 snoRNA gene isolated from a Chlamydomonas reinhardtii (CRE:) genomic library contains putative pol III-specific transcription signals similar to those of RNA polymerase III-specific small nuclear (sn)RNA genes of higher plants. The 222 nt long CRE: U3 snoRNA was immunoprecipitated by anti-gamma-mpppN antisera, bu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.15.2959
更新日期:2000-08-01 00:00:00
abstract::Dimerization of HIV-1 genomic RNA is an essential step of the viral cycle, initiated at a conserved stem-loop structure which forms a 'kissing complex' involving loop-loop interactions (dimerization initiation site, DIS). A 19mer RNA oligonucleotide (DIS-19) has been synthesized which forms a stable symmetrical dimer ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.15.3567
更新日期:1998-08-01 00:00:00