Microcomputer programs for back translation of protein to DNA sequences and analysis of ambiguous DNA sequences.

abstract：:The specific DNA binding ligand netropsin selectively blocks dA-dT base pairs in clusters containing two or more consecutive thymine residues at the dNAase I cleavage sites of DNA. Using CD and UV absorption measurements it is shown, that at various ratios of netropsin to nucleotide concentrations and even at satuatio...

journal_title：Nucleic acids research

authors： Zimmer C,Luck G,Nüske R

更新日期：1980-07-11 00:00:00

abstract：:Fast viral adaptation and the implication of this rapid evolution in the emergence of several new infectious diseases have turned this issue into a major challenge for various research domains. Indeed, viruses are involved in the development of a wide range of pathologies and understanding how viruses and host cells i...

journal_title：Nucleic acids research

authors： Palmeira L,Penel S,Lotteau V,Rabourdin-Combe C,Gautier C

更新日期：2011-01-01 00:00:00

abstract：:The crystal structure of the RNA/DNA hybrid r(GAAGAGAAGC). d(GCTTCTCTTC) has been solved and refined at 2.5 A resolution. The refinement procedure converged at R = 0.181 for all reflections in the range 20.0-2.5 A. In the crystal, the RNA/DNA hybrid duplex has an A' conformation with all but one of the nucleotide suga...

journal_title：Nucleic acids research

authors： Conn GL,Brown T,Leonard GA

更新日期：1999-01-15 00:00:00

abstract：:The Network of Cancer Genes (NCG) collects and integrates data on 736 human genes that are mutated in various types of cancer. For each gene, NCG provides information on duplicability, orthology, evolutionary appearance and topological properties of the encoded protein in a comprehensive version of the human protein-p...

journal_title：Nucleic acids research

authors： Syed AS,D'Antonio M,Ciccarelli FD

更新日期：2010-01-01 00:00:00

abstract：:Cell surface mannose 6-phosphate/insulin-like growth factor II receptors (M6P/IGF2R) bind and target exogenous insulin-like growth factor II (IGF2) to the prelysosomes where it is degraded. Loss of heterozygosity (LOH) for M6P/IGF2R is found in cancers, with mutational inactivation of the remaining allele. We exploite...

journal_title：Nucleic acids research

authors： Huang Z,Wen Y,Shandilya R,Marks JR,Berchuck A,Murphy SK

更新日期：2006-01-23 00:00:00

abstract：:The transmission of genetic information relies on Watson-Crick base pairing between nucleoside phosphates and template bases in template-primer complexes. Enzyme-free primer extension is the purest form of the transmission process, without any chaperon-like effect of polymerases. This simple form of copying of sequenc...

journal_title：Nucleic acids research

authors： Kervio E,Claasen B,Steiner UE,Richert C

更新日期：2014-06-01 00:00:00

abstract：:The chromatin-remodelling complex B-WICH, comprised of William syndrome transcription factor, the ATPase SNF2h and nuclear myosin, specifically activates RNA polymerase III transcription of the 5S rRNA and 7SL genes. However, the underlying mechanism is unknown. Using high-resolution MN walking we demonstrate here tha...

journal_title：Nucleic acids research

authors： Sadeghifar F,Böhm S,Vintermist A,Östlund Farrants AK

更新日期：2015-05-19 00:00:00

abstract：:The TGGCA protein, the chicken homologue of HeLa cell NF-I, was purified to homogeneity from liver tissue by a procedure which includes preparative mobility shift electrophoresis (PMSE) as the final step. PMSE was here adjusted for the isolation of the TGGCA protein, but can be used as a general method to characterize...

journal_title：Nucleic acids research

authors： Rupp RA,Sippel AE

更新日期：1987-12-10 00:00:00

abstract：:Assessment of the fidelity of gene expression is crucial to understand cell homeostasis. Here we present a highly sensitive method for the systematic Quantification of Rare Amino acid Substitutions (QRAS) using absolute quantification by targeted mass spectrometry after chromatographic enrichment of peptides with miss...

journal_title：Nucleic acids research

authors： Garofalo R,Wohlgemuth I,Pearson M,Lenz C,Urlaub H,Rodnina MV

更新日期：2019-04-08 00:00:00

abstract：:Variable (diversity) joining [V(D)J] recombination of immune gene loci proceeds in an ordered manner with D to J portions recombining first and then an upstream V joins that recombinant. We present evidence that the non-core domain of recombination activating gene (RAG) protein 2 is involved in the regulation of recom...

journal_title：Nucleic acids research

authors： Curry JD,Schlissel MS

更新日期：2008-10-01 00:00:00

abstract：:Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast ...

journal_title：Nucleic acids research

authors： Habu T,Taki T,West A,Nishimune Y,Morita T

更新日期：1996-02-01 00:00:00

abstract：:When B cells differentiate into plasma cells, there is a large increase in the cellular content of mRNA coding for immunoglobulin. This increase cannot be fully accounted for by the increase in rate of transcription of the genes. We have investigated the possibility that the half-life of mu heavy chain mRNA increases ...

journal_title：Nucleic acids research

authors： Cox A,Emtage JS

更新日期：1989-12-25 00:00:00

abstract：:Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....

journal_title：Nucleic acids research

authors： Hinton DM,Musso RE

更新日期：1982-08-25 00:00:00

abstract：:AvrBs3, the founding member of the Xanthomonas transcription-activator-like effectors (TALEs), is translocated into the plant cell where it localizes to the nucleus and acts as transcription factor. The DNA-binding domain of AvrBs3 consists of 17.5 nearly-identical 34 amino acid-repeats. Each repeat specifies binding ...

journal_title：Nucleic acids research

authors： Schreiber T,Bonas U

更新日期：2014-06-01 00:00:00

abstract：:We have investigated loop-induced structural perturbation of the stem structure in hairpins d(GAATTCXnGAATTC) (X = A, T and n = 3, 4, 5 and 6) that contain an EcoRI restriction site in close proximity to the hairpin loop. Oligonucleotides containing either a T3 or a A3 loop were not hydrolyzed by the restriction enzym...

journal_title：Nucleic acids research

authors： Germann MW,Kalisch BW,Lundberg P,Vogel HJ,van de Sande JH

更新日期：1990-03-25 00:00:00

abstract：:Replacement of alpha-, beta- and gamma-phosphate groups in 2'-deoxynucleoside 5'-triphosphates (dNTP) with phosphonate groups yields a new set of dNTP mimics with potential biological and therapeutic applications. Here, we describe the synthesis of 15 new dNTPs modified at alpha-, beta- and gamma-phosphates containing...

journal_title：Nucleic acids research

authors： Alexandrova LA,Skoblov AY,Jasko MV,Victorova LS,Krayevsky AA

更新日期：1998-02-01 00:00:00

abstract：:Systematic structure probing experiments (e.g. SHAPE) of RNA mutants such as the mutate-and-map (MaM) protocol give us a direct access into the genetic robustness of ncRNA structures. Comparative studies of homologous sequences provide a distinct, yet complementary, approach to analyze structural and functional proper...

journal_title：Nucleic acids research

authors： Reinharz V,Ponty Y,Waldispühl J

更新日期：2016-06-20 00:00:00

abstract：:Large expansions of the trinucleotide repeat GAA*TTC within the first intron of the X25 (frataxin) gene cause Friedreich's ataxia, the most common inherited ataxia. Expansion leads to reduced levels of frataxin mRNA in affected individuals. Here we show that GAA*TTC tracts, in the absence of any other frataxin gene se...

journal_title：Nucleic acids research

authors： Grabczyk E,Usdin K

更新日期：2000-07-15 00:00:00

abstract：:The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms of cancer cell resistance to irinotecan remains poorly understood. Here, we generated colorectal ...

journal_title：Nucleic acids research

authors： Meisenberg C,Ashour ME,El-Shafie L,Liao C,Hodgson A,Pilborough A,Khurram SA,Downs JA,Ward SE,El-Khamisy SF

更新日期：2017-02-17 00:00:00

abstract：:Single-molecule force spectroscopy provides insight into how proteins bind to and move along DNA. Such studies often embed a single-stranded (ss) DNA region within a longer double-stranded (ds) DNA molecule. Yet, producing these substrates remains laborious and inefficient, particularly when using the traditional thre...

journal_title：Nucleic acids research

authors： Okoniewski SR,Uyetake L,Perkins TT

更新日期：2017-10-13 00:00:00

abstract：:The Mouse Genome Database (MGD) (http://www.informatics.jax.org) one component of a community database resource for the laboratory mouse, a key model organism for interpreting the human genome and for understanding human biology. MGD strives to provide an extensively integrated information resource with experimental d...

journal_title：Nucleic acids research

authors： Blake JA,Richardson JE,Bult CJ,Kadin JA,Eppig JT,Mouse Genome Database Group.

更新日期：2003-01-01 00:00:00

abstract：:Loss-of-function in melanocortin 1 receptor (MC1R), a GS protein-coupled receptor that regulates signal transduction through cAMP and protein kinase A (PKA) in melanocytes, is a major inherited melanoma risk factor. Herein, we report a novel cAMP-mediated response for sensing and responding to UV-induced DNA damage re...

journal_title：Nucleic acids research

authors： Jarrett SG,Wolf Horrell EM,D'Orazio JA

更新日期：2016-12-15 00:00:00

abstract：:The DNA double helix undergoes an 'overstretching' transition in a narrow force range near 65 pN. Despite numerous studies the basic question of whether the strands are separated or not remains controversial. Here we show that overstretching in fact involves two distinct types of double-helix reorganization: slow hyst...

journal_title：Nucleic acids research

authors： Fu H,Chen H,Marko JF,Yan J

更新日期：2010-09-01 00:00:00

abstract：:The active 3D conformation of the spliceosome's catalytic U2/U6 RNA core is stabilised by a network of secondary and tertiary RNA interactions, but also depends on spliceosomal proteins for its formation. To determine the contribution towards splicing of specific RNA secondary and tertiary interactions in the U2/U6 RN...

journal_title：Nucleic acids research

authors： Bao P,Boon KL,Will CL,Hartmuth K,Lührmann R

更新日期：2018-12-14 00:00:00

abstract：:Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...

journal_title：Nucleic acids research

authors： Wang G,Xu X,Pace B,Dean DA,Glazer PM,Chan P,Goodman SR,Shokolenko I

更新日期：1999-07-01 00:00:00

abstract：:Reverse transcription of HIV-1 RNA is primed by a tRNA3(Lys) molecule bound at the primer binding site (PBS). Complex intermolecular interactions were proposed between tRNA3(Lys) and the RNA of the HIV-1 Mal isolate. Recently, an alternative interaction was proposed between the TPsiC stem of tRNA3(Lys) and a primer ac...

journal_title：Nucleic acids research

authors： Goldschmidt V,Ehresmann C,Ehresmann B,Marquet R

更新日期：2003-02-01 00:00:00

abstract：:The webPDBinder (http://pdbinder.bio.uniroma2.it/PDBinder) is a web server for the identification of small ligand-binding sites in a protein structure. webPDBinder searches a protein structure against a library of known binding sites and a collection of control non-binding pockets. The number of similarities identifie...

journal_title：Nucleic acids research

authors： Bianchi V,Mangone I,Ferrè F,Helmer-Citterich M,Ausiello G

更新日期：2013-07-01 00:00:00

abstract：:Eukaryotic RNases H from Saccharomyces cerevisiae , Schizosaccharomyces pombe and Crithidia fasciculata , unlike the related Escherichia coli RNase HI, contain a non-RNase H domain with a common motif. Previously we showed that S.cerevisiae RNase H1 binds to duplex RNAs (either RNA-DNA hybrids or double-stranded RNA) ...

journal_title：Nucleic acids research

authors： Cerritelli SM,Fedoroff OY,Reid BR,Crouch RJ

更新日期：1998-04-01 00:00:00

abstract：:The human embryonal carcinoma cell line NEC14 can be induced to differentiate by the addition of 10(-2)M N,N'-hexamethylene-bis-acetamide (HMBA). A subtractive cDNA library specific to undifferentiated NEC14 cells was constructed using oligo(dT)30-Latex and polymerase chain reaction (PCR). The method was designed to i...

journal_title：Nucleic acids research

authors： Hara E,Kato T,Nakada S,Sekiya S,Oda K

更新日期：1991-12-01 00:00:00

abstract：:We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction with the synthetic D...

journal_title：Nucleic acids research

authors： Osinga KA,Van der Bliek AM,Van der Horst G,Groot Koerkamp MJ,Tabak HF,Veeneman GH,Van Boom JH

更新日期：1983-12-20 00:00:00