A gender-specific mRNA encoding a cytotoxic ribonuclease contains a 3' UTR of unusual length and structure.

abstract：:DNA circuit is a versatile and highly-programmable toolbox which can potentially be used for the autonomous sensing of dynamic events, such as biomolecular interactions. However, the experimental implementation of in silico circuit designs has been hindered by the problem of circuit leakage. Here, we systematically an...

journal_title：Nucleic acids research

authors： Ang YS,Tong R,Yung LY

更新日期：2016-08-19 00:00:00

abstract：:The hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for the analysis of DNA variation. In this paper, we study how probe immobilization at surfaces, specifically probe density, influences the kinetics of target capture using surface plasmon resonance (SPR) s...

journal_title：Nucleic acids research

authors： Peterson AW,Heaton RJ,Georgiadis RM

更新日期：2001-12-15 00:00:00

abstract：:The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity...

journal_title：Nucleic acids research

authors： Felber BK,Gerber-Huber S,Meier C,May FE,Westley B,Weber R,Ryffel GU

更新日期：1981-06-11 00:00:00

abstract：:We have modeled local DNA sequence parameters to search for DNA architectural motifs involved in transcription regulation and promotion within the Xenopus laevis ribosomal gene promoter and the intergenic spacer (IGS) sequences. The IGS was found to be shaped into distinct topological domains. First, intrinsic bends s...

journal_title：Nucleic acids research

authors： Roux-Rouquie M,Marilley M

更新日期：2000-09-15 00:00:00

abstract：:Purified 15 S globin mRNA-protein (mRNP) complexes obtained by EDTA dissociation of duck reticulocytes polyribosomes were digested with the calcium dependant Staphylococcus aureus nuclease (EC 3. 1. 4. 7.). 25% of the globin mRNA sequences were resistant to extensive nuclease digestion as determined by TCA precipitati...

journal_title：Nucleic acids research

authors： Goldenberg S,Vincent A,Scherrer K

更新日期：1979-06-25 00:00:00

abstract：:LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally he...

journal_title：Nucleic acids research

authors： Rotman G,Itin A,Keshet E

更新日期：1986-01-24 00:00:00

abstract：:A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends. ...

journal_title：Nucleic acids research

authors： Steponaviciene D,Maneliene Z,Petrusyte M,Janulaitis A

更新日期：1999-07-01 00:00:00

abstract：:Myotonic dystrophy type 1 (DM1) is an autosomal dominant multisystemic disorder caused by expansion of CTG triplet repeats in 3'-untranslated region of DMPK gene. The pathomechanism of DM1 is driven by accumulation of toxic transcripts containing expanded CUG repeats (CUG(exp)) in nuclear foci which sequester several ...

journal_title：Nucleic acids research

authors： Wojtkowiak-Szlachcic A,Taylor K,Stepniak-Konieczna E,Sznajder LJ,Mykowska A,Sroka J,Thornton CA,Sobczak K

更新日期：2015-03-31 00:00:00

abstract：:X-linked agammaglobulinemia (XLA) is an immunodeficiency caused by mutations in the gene coding for Bruton's agammaglobulinemia tyrosine kinase (BTK). A database (BTKbase) of BTK mutations has been compiled and the recent update lists 368 entries from 318 unrelated families showing 228 unique molecular events. In addi...

journal_title：Nucleic acids research

authors： Vihinen M,Belohradsky BH,Haire RN,Holinski-Feder E,Kwan SP,Lappalainen I,Lehväslaiho H,Lester T,Meindl A,Ochs HD,Ollila J,Vorechovsky I,Weiss M,Smith CI

更新日期：1997-01-01 00:00:00

abstract：:ORegAnno is an open-source, open-access database and literature curation system for community-based annotation of experimentally identified DNA regulatory regions, transcription factor binding sites and regulatory variants. The current release comprises 30 145 records curated from 922 publications and describing regul...

journal_title：Nucleic acids research

authors： Griffith OL,Montgomery SB,Bernier B,Chu B,Kasaian K,Aerts S,Mahony S,Sleumer MC,Bilenky M,Haeussler M,Griffith M,Gallo SM,Giardine B,Hooghe B,Van Loo P,Blanco E,Ticoll A,Lithwick S,Portales-Casamar E,Donaldson IJ,

更新日期：2008-01-01 00:00:00

abstract：:The HUGO Gene Nomenclature Committee (HGNC) based at EMBL's European Bioinformatics Institute (EMBL-EBI) assigns unique symbols and names to human genes. There are over 42,000 approved gene symbols in our current database of which over 19 000 are for protein-coding genes. While we still update placeholder and problema...

journal_title：Nucleic acids research

authors： Tweedie S,Braschi B,Gray K,Jones TEM,Seal RL,Yates B,Bruford EA

更新日期：2021-01-08 00:00:00

abstract：:Modular primers are strings of three contiguously annealed unligated oligonucleotides (modules) as short as 5- or 6-mers, selected from a presynthesized library. It was previously found that such strings can prime DNA sequencing reactions specifically, thus eliminating the need for the primer synthesis step in DNA seq...

journal_title：Nucleic acids research

authors： Beskin AD,Zevin-Sonkin D,Sobolev IA,Ulanovsky LE

更新日期：1995-08-11 00:00:00

abstract：:The molecular basis for the DNA binding specificity of the thyroid transcription factor 1 homeodomain (TTF-1HD) has been investigated. Methylation and ethylation interference experiments show that the TTF-1HD alone recapitulates the DNA binding properties of the entire protein. Studies carried out with mutant derivati...

journal_title：Nucleic acids research

authors： Damante G,Fabbro D,Pellizzari L,Civitareale D,Guazzi S,Polycarpou-Schwartz M,Cauci S,Quadrifoglio F,Formisano S,Di Lauro R

更新日期：1994-08-11 00:00:00

abstract：:Two of the tRNA's found in rabbit reticulocytes are substrates for a post-transcriptional modification leading to the incorporation of guanine into the polynucleotide chain. The major guanylated tRNA was previously identified as tRNA (His). In the present report we show that the minor guanylated tRNA is tRNA (Asn), an...

journal_title：Nucleic acids research

authors： Farkas WR,Chernoff D

更新日期：1976-10-01 00:00:00

abstract：:Natural transformation is a major mechanism of horizontal gene transfer in bacteria that depends on DNA recombination. RecA is central to the homologous recombination pathway, catalyzing DNA strand invasion and homology search. DprA was shown to be a key binding partner of RecA acting as a specific mediator for its lo...

journal_title：Nucleic acids research

authors： Lisboa J,Andreani J,Sanchez D,Boudes M,Collinet B,Liger D,van Tilbeurgh H,Guérois R,Quevillon-Cheruel S

更新日期：2014-06-01 00:00:00

abstract：:We have demonstrated that the DNA sequence between two triplex-forming polypurine.polypyrimidine (Pu.Py) tracts was protected from DNA modifying enzymes upon formation of triplex DNA structures with an oligodeoxyribonucleotide in which two triplex-forming Pu or Py tracts were placed at the termini (triplex-bridge form...

journal_title：Nucleic acids research

authors： Kiyama R,Oishi M

更新日期：1995-02-11 00:00:00

abstract：:Human papillomavirus 16 (HPV-16), which is involved in genital carcinogenesis, contains an enhancer of transcription that is activated by cellular factors rather than by the viral E2 proteins. The activity resides on a 232 bp segment with 5 binding sites for nuclear factor 1 (NF1), 2 for AP1, and 1 for steroid recepto...

journal_title：Nucleic acids research

authors： Chong T,Chan WK,Bernard HU

更新日期：1990-02-11 00:00:00

abstract：:Kinetoplast mitochondrial RNA editing is the developmentally regulated post-transcriptional process of uridine insertion and deletion in mRNAs directed by short guide RNAs (gRNAs), which creates functional mRNAs. Two mechanisms are proposed: transesterification which predicts gRNA/mRNA chimeric intermediates, and enzy...

journal_title：Nucleic acids research

authors： Riley GR,Myler PJ,Stuart K

更新日期：1995-02-25 00:00:00

abstract：:To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore. These dUTP derivatives and commercial Cy3- and Cy5-dUTP were stud...

journal_title：Nucleic acids research

authors： Zasedateleva OA,Vasiliskov VA,Surzhikov SA,Kuznetsova VE,Shershov VE,Guseinov TO,Smirnov IP,Yurasov RA,Spitsyn MA,Chudinov AV

更新日期：2018-07-06 00:00:00

abstract：:A detailed scheme of the Peptidyl Transferase Centre of bacterial ribosomes is proposed by summarizing the literature data on the substrate specificity of the acceptor and donor sites. According to the proposed scheme only the elements of the donor and acceptor having a stable structure bind with the ribosome. The pre...

journal_title：Nucleic acids research

authors： Krayevsky AA,Kukhanova MK,Gottikh BP

更新日期：1975-12-01 00:00:00

abstract：:Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...

journal_title：Nucleic acids research

authors： Pavlov NA,Cherny DI,Nazimov IV,Slesarev AI,Subramaniam V

更新日期：2002-02-01 00:00:00

abstract：:The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bact...

journal_title：Nucleic acids research

authors： Li XT,Thomason LC,Sawitzke JA,Costantino N,Court DL

更新日期：2013-12-01 00:00:00

abstract：:The Arabidopsis Information Resource (TAIR, http://arabidopsis.org) is the model organism database for the fully sequenced and intensively studied model plant Arabidopsis thaliana. Data in TAIR is derived in large part from manual curation of the Arabidopsis research literature and direct submissions from the research...

journal_title：Nucleic acids research

authors： Swarbreck D,Wilks C,Lamesch P,Berardini TZ,Garcia-Hernandez M,Foerster H,Li D,Meyer T,Muller R,Ploetz L,Radenbaugh A,Singh S,Swing V,Tissier C,Zhang P,Huala E

更新日期：2008-01-01 00:00:00

abstract：:Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here,...

journal_title：Nucleic acids research

authors： Hör J,Di Giorgio S,Gerovac M,Venturini E,Förstner KU,Vogel J

更新日期：2020-09-18 00:00:00

abstract：:Protein structures are an ensemble of atoms determined experimentally mostly by X-ray crystallography or Nuclear Magnetic Resonance. Studying 3D protein structures is a key point for better understanding protein function at a molecular level. We propose a set of accurate tools, for analysing protein structures, based ...

journal_title：Nucleic acids research

authors： Esque J,Léonard S,de Brevern AG,Oguey C

更新日期：2013-07-01 00:00:00

abstract：:Mature tRNAPhe from Escherichia coli and the transcript of its gene lacking modified nucleotides were compared by a variety of physical techniques. Melting experiments revealed that at a low Mg2+level the transcript was partially denatured, while the mature tRNA possessed intact tertiary interactions. Mg2+binding to b...

journal_title：Nucleic acids research

authors： Serebrov V,Vassilenko K,Kholod N,Gross HJ,Kisselev L

更新日期：1998-06-01 00:00:00

abstract：:CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably re...

journal_title：Nucleic acids research

authors： Fujita R,Otake K,Arimura Y,Horikoshi N,Miya Y,Shiga T,Osakabe A,Tachiwana H,Ohzeki J,Larionov V,Masumoto H,Kurumizaka H

更新日期：2015-05-26 00:00:00

abstract：:Gene expression is a multi-step process involving many layers of regulation. The main regulators of the pathway are DNA and RNA binding proteins. While over the years, a large number of DNA and RNA binding proteins have been identified and extensively studied, it is still expected that many other proteins, some with y...

journal_title：Nucleic acids research

authors： Paz I,Kligun E,Bengad B,Mandel-Gutfreund Y

更新日期：2016-07-08 00:00:00

abstract：:Human foamy or spuma virus (HFV) codes for a distinct set of pol gen products. To determine the minimal requirements for the HFV enzymatic activities, defined residues of the reverse transcriptase (RT) and ribo-nuclease H (RNase H) domain of the HFV pol gene were mutated by site-specific PCR mutagenesis. The mutant ge...

journal_title：Nucleic acids research

authors： Kögel D,Aboud M,Flügel RM

更新日期：1995-07-25 00:00:00

abstract：:The Rice Proteome Database is the first detailed database to describe the proteome of rice. The current release contains 21 reference maps based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of proteins from rice tissues and subcellular compartments. These reference maps comprise 11 941 identified pr...

journal_title：Nucleic acids research

authors： Komatsu S,Kojima K,Suzuki K,Ozaki K,Higo K

更新日期：2004-01-01 00:00:00