当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants.

Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants.

Abstract：

:The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco plants which had been standardized physiologically as much as possible. The presence of adjacent pUC plasmid sequences did not affect the expression of the SSU301 gene. In an attempt to reduce the between-transformant variability in expression, the SSU301 gene was introduced into tobacco surrounded by 10kb of 5' and 13 kb of 3' DNA sequences which normally flank SSU301 in petunia. The longer flanking regions did not reduce the between-transformant variability of SSU301 gene expression.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Dean C,Jones J,Favreau M,Dunsmuir P,Bedbrook J

doi

10.1093/nar/16.19.9267

subject

Has Abstract

pub_date

1988-10-11 00:00:00

pages

9267-83

issue

19

eissn

0305-1048

issn

1362-4962

journal_volume

16

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress.

    abstract：:We examined the mechanism regulating the cellular levels of PNKP, the major kinase/phosphatase involved in the repair of oxidative DNA damage, and find that it is controlled by ATM phosphorylation and ubiquitylation-dependent proteasomal degradation. We discovered that ATM-dependent phosphorylation of PNKP at serines ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks909

    authors： Parsons JL,Khoronenkova SV,Dianova II,Ternette N,Kessler BM,Datta PK,Dianov GL

    更新日期：2012-12-01 00:00:00

  • Primary sequence and partial secondary structure of the 12S kinetoplast (mitochondrial) ribosomal RNA from Leishmania tarentolae: conservation of peptidyl-transferase structural elements.

    abstract：:The sequence of the 1173 nt 12S kinetoplast ribosomal RNA from Leishmania tarentolae was determined from the maxicircle DNA sequence, and the 5' and 3' ends localized by primer runoff and S1 nuclease protection experiments. The gene was shown to be free of introns by S1 nuclease analysis. A partial secondary structure...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/13.7.2337

    authors： de la Cruz VF,Simpson AM,Lake JA,Simpson L

    更新日期：1985-04-11 00:00:00

  • Rational engineering of the Neurospora VS ribozyme to allow substrate recognition via different kissing-loop interactions.

    abstract：:The Neurospora VS ribozyme is a catalytic RNA that has the unique ability to specifically recognize and cleave a stem-loop substrate through formation of a highly stable kissing-loop interaction (KLI). In order to explore the engineering potential of the VS ribozyme to cleave alternate substrates, we substituted the w...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw401

    authors： Lacroix-Labonté J,Girard N,Dagenais P,Legault P

    更新日期：2016-08-19 00:00:00

  • A nuclear protein with enhanced binding to methylated Sp1 sites in the AIDS virus promoter.

    abstract：:We report here the discovery of HMBP, a protein in nuclei of human T-helper lymphocytes and other human cell types, which binds with enhanced affinity to a promoter element in the HIV-1 long terminal repeat when that element is methylated at CpGs, the target site of the human DNA methyltransferase. This promoter eleme...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.24.5786

    authors： Joel P,Shao W,Pratt K

    更新日期：1993-12-11 00:00:00

  • Identification of members of several homeobox genes in a planarian using a ligation-mediated polymerase chain reaction technique.

    abstract：:I have used a novel single-sided specific polymerase chain reaction (PCR) strategy inspired by ligation-mediated PCR to clone fragments of divergent homeobox genes from a flatworm, the planarian Polycelis nigra. Eight homeobox-containing fragments were amplified, belonging to the Hox, msh, NK-1 and NK-2 classes. Toget...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/24.8.1547

    authors： Balavoine G

    更新日期：1996-04-15 00:00:00

  • Identification of rare DNA variants in mitochondrial disorders with improved array-based sequencing.

    abstract：:A common goal in the discovery of rare functional DNA variants via medical resequencing is to incur a relatively lower proportion of false positive base-calls. We developed a novel statistical method for resequencing arrays (SRMA, sequence robust multi-array analysis) to increase the accuracy of detecting rare variant...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq750

    authors： Wang W,Shen P,Thiyagarajan S,Lin S,Palm C,Horvath R,Klopstock T,Cutler D,Pique L,Schrijver I,Davis RW,Mindrinos M,Speed TP,Scharfe C

    更新日期：2011-01-01 00:00:00

  • PathPred: an enzyme-catalyzed metabolic pathway prediction server.

    abstract：:The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq318

    authors： Moriya Y,Shigemizu D,Hattori M,Tokimatsu T,Kotera M,Goto S,Kanehisa M

    更新日期：2010-07-01 00:00:00

  • Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21.

    abstract：:We report the molecular cloning and characterization of a cDNA derived from a zebrafish gene (ZF-21) related to the mouse homeobox containing gene Hox2.1. Interesting information about the differential conservation of various domains was gained from comparisons between the putative protein sequences from ZF-21 (275 am...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.19.9097

    authors： Njølstad PR,Molven A,Hordvik I,Apold J,Fjose A

    更新日期：1988-10-11 00:00:00

  • Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).

    abstract：:Use of the polymerase chain reaction (PCR) to amplify variable numbers of tandem repeat (VNTR) loci has become widely used in genetic typing. Unfortunately, preferential amplification of small allelic products relative to large allelic products may result in incorrect or ambiguous typing in a heterozygous sample. The ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.15.3050

    authors： Demers DB,Curry ET,Egholm M,Sozer AC

    更新日期：1995-08-11 00:00:00

  • Molecular footprints of human immunoglobulin gene evolution: a new sequence family.

    abstract：:Analysis of the human VK (ref. 2) gene locus led to the detection of a new sequence family (L sequences). Its copy number is in the range of 10(2). The L sequences, which are about 500 bp long, are found as part of the 3' flanking regions of a clustered set of human VKI genes but they occur also separate from the gene...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.13.5265

    authors： Straubinger B,Pech M,Mühlebach K,Jaenichen HR,Bauer HG,Zachau HG

    更新日期：1984-07-11 00:00:00

  • MutSβ and histone deacetylase complexes promote expansions of trinucleotide repeats in human cells.

    abstract：:Trinucleotide repeat (TNR) expansions cause at least 17 heritable neurological diseases, including Huntington's disease. Expansions are thought to arise from abnormal processing of TNR DNA by specific trans-acting proteins. For example, the DNA repair complex MutSβ (MSH2-MSH3 heterodimer) is required in mice for on-go...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks810

    authors： Gannon AM,Frizzell A,Healy E,Lahue RS

    更新日期：2012-11-01 00:00:00

  • Base-flipping dynamics from an intrahelical to an extrahelical state exerted by thymine DNA glycosylase during DNA repair process.

    abstract：:Thymine DNA glycosylase (TDG) is a DNA repair enzyme that excises a variety of mismatched or damaged nucleotides (nts), e.g. dU, dT, 5fC and 5caC. TDG is shown to play essential roles in maintaining genome integrity and correctly programming epigenetic modifications through DNA demethylation. After locating the lesion...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky386

    authors： Da LT,Yu J

    更新日期：2018-06-20 00:00:00

  • Distribution of DNA-condensing protein complexes in the adenovirus core.

    abstract：:Genome packing in adenovirus has long evaded precise description, since the viral dsDNA molecule condensed by proteins (core) lacks icosahedral order characteristic of the virus protein coating (capsid). We show that useful insights regarding the organization of the core can be inferred from the analysis of spatial di...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv187

    authors： Pérez-Berná AJ,Marion S,Chichón FJ,Fernández JJ,Winkler DC,Carrascosa JL,Steven AC,Šiber A,San Martín C

    更新日期：2015-04-30 00:00:00

  • Tracts of A.T base pairs retard the electrophoretic mobility of short DNA duplexes.

    abstract：:An investigation of the mobility of short duplexes of DNA -octamers and decamers-on polyacrylamide gels is presented, showing that molecules containing less than one helical turn exhibit sequence dependent mobilities. Analysis of chains with different sequences indicates that any arrangement of two or more adjacent A....

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.14.6803

    authors： Chen JH,Seeman NC,Kallenbach NR

    更新日期：1988-07-25 00:00:00

  • InvFEST, a database integrating information of polymorphic inversions in the human genome.

    abstract：:The newest genomic advances have uncovered an unprecedented degree of structural variation throughout genomes, with great amounts of data accumulating rapidly. Here we introduce InvFEST (http://invfestdb.uab.cat), a database combining multiple sources of information to generate a complete catalogue of non-redundant hu...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1122

    authors： Martínez-Fundichely A,Casillas S,Egea R,Ràmia M,Barbadilla A,Pantano L,Puig M,Cáceres M

    更新日期：2014-01-01 00:00:00

  • Experimental maps of DNA structure at nucleotide resolution distinguish intrinsic from protein-induced DNA deformations.

    abstract：:Recognition of DNA by proteins depends on DNA sequence and structure. Often unanswered is whether the structure of naked DNA persists in a protein-DNA complex, or whether protein binding changes DNA shape. While X-ray structures of protein-DNA complexes are numerous, the structure of naked cognate DNA is seldom availa...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky033

    authors： Azad RN,Zafiropoulos D,Ober D,Jiang Y,Chiu TP,Sagendorf JM,Rohs R,Tullius TD

    更新日期：2018-03-16 00:00:00

  • DNA compaction by the bacteriophage protein Cox studied on the single DNA molecule level using nanofluidic channels.

    abstract：:The Cox protein from bacteriophage P2 forms oligomeric filaments and it has been proposed that DNA can be wound up around these filaments, similar to how histones condense DNA. We here use fluorescence microscopy to study single DNA-Cox complexes in nanofluidic channels and compare how the Cox homologs from phages P2 ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw352

    authors： Frykholm K,Berntsson RP,Claesson M,de Battice L,Odegrip R,Stenmark P,Westerlund F

    更新日期：2016-09-06 00:00:00

  • Conserved thermochemistry of guanosine nucleophile binding for structurally distinct group I ribozymes.

    abstract：:We report thermodynamic values for binding of the guanosine nucleophile to the ribozyme derived from the Anabaena group I intron, and find that they are similar to those measured previously for the structurally distinct Tetrahymena ribozyme. The free energy of binding guanosine 5'-monophosphate (pG) at 30 degrees C is...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/24.19.3722

    authors： Kuo LY,Cech TR

    更新日期：1996-10-01 00:00:00

  • The germ line limited M element of Tetrahymena is targeted for elimination from the somatic genome by a homology-dependent mechanism.

    abstract：:A RNA interference (RNAi) like mechanism is involved in elimination of thousands of DNA segments from the developing somatic macronucleus of Tetrahymena, yet how specific internal eliminated sequences (IESs) are recognized remains to be fully elucidated. To define requirements for DNA rearrangement, we performed mutag...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl699

    authors： Kowalczyk CA,Anderson AM,Arce-Larreta M,Chalker DL

    更新日期：2006-01-01 00:00:00

  • SplicePort--an interactive splice-site analysis tool.

    abstract：:SplicePort is a web-based tool for splice-site analysis that allows the user to make splice-site predictions for submitted sequences. In addition, the user can also browse the rich catalog of features that underlies these predictions, and which we have found capable of providing high classification accuracy on human s...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm407

    authors： Dogan RI,Getoor L,Wilbur WJ,Mount SM

    更新日期：2007-07-01 00:00:00

  • Transcription through the yeast origin of replication ARS1 ends at the ABFI binding site and affects extrachromosomal maintenance of minichromosomes.

    abstract：:When the function of origins of replication in yeast was compromised by placing ARS sequences downstream of strong promoters, ARS activity might have been affected either by transcription or by an altered chromatin configuration induced by the construct. To distinguish between these possibilities, derivatives of the y...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/22.19.3904

    authors： Tanaka S,Halter D,Livingstone-Zatchej M,Reszel B,Thoma F

    更新日期：1994-09-25 00:00:00

  • Mitome: dynamic and interactive database for comparative mitochondrial genomics in metazoan animals.

    abstract：:Mitome is a specialized mitochondrial genome database designed for easy comparative analysis of various features of metazoan mitochondrial genomes such as base frequency, A+T skew, codon usage and gene arrangement pattern. A particular function of the database is the automatic reconstruction of phylogenetic relationsh...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm763

    authors： Lee YS,Oh J,Kim YU,Kim N,Yang S,Hwang UW

    更新日期：2008-01-01 00:00:00

  • Statistical evaluation of differential expression on cDNA nylon arrays with replicated experiments.

    abstract：:In this paper we focus on the detection of differentially expressed genes according to changes in hybridization signals using statistical tests. These tests were applied to 14 208 zebrafish cDNA clones that were immobilized on a nylon support and hybridized with radioactively labeled target mRNA from wild-type and lit...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/29.23.e117

    authors： Herwig R,Aanstad P,Clark M,Lehrach H

    更新日期：2001-12-01 00:00:00

  • Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis.

    abstract：:Embryonic stem cells (ESCs) and meiosis are featured by relatively higher frequent homologous recombination associated with DNA double strand breaks (DSB) repair. Here, we show that Pold3 plays important roles in DSB repair, telomere maintenance and genomic stability of both ESCs and spermatocytes in mice. By attempti...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky098

    authors： Zhou Z,Wang L,Ge F,Gong P,Wang H,Wang F,Chen L,Liu L

    更新日期：2018-04-20 00:00:00

  • Defining the sequence recognized with BmFTZ-F1, a sequence specific DNA binding factor in the silkworm, Bombyx mori, as revealed by direct sequencing of bound oligonucleotides and gel mobility shift competition analysis.

    abstract：:BmFTZ-F1 is a Bombyx mori homologue of FTZ-F1, a positive regulator of the fushi tarazu gene of Drosophila melanogaster. In order to determine the sequence recognized with this factor, we made three sets of oligonucleotide mixture which contain 4 possible nucleotides at different positions within the previously propos...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.13.3689

    authors： Ueda H,Hirose S

    更新日期：1991-07-11 00:00:00

  • Detection of oligonucleotide hybridization at femtomolar level and sequence-specific gene analysis of the Arabidopsis thaliana leaf extract with an ultrasensitive surface plasmon resonance spectrometer.

    abstract：:A flow-injection (FI) device is combined, through the use of a low-volume (4 microl) flow cell, with an ultrasensitive surface plasmon resonance (SPR) spectrometer equipped with a bi-cell photodiode detector. The application of this novel FI-SPR device for sequence-specific ultratrace analysis of oligodeoxynucleotides...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gnf072

    authors： Song F,Zhou F,Wang J,Tao N,Lin J,Vellanoweth RL,Morquecho Y,Wheeler-Laidman J

    更新日期：2002-07-15 00:00:00

  • Pseudomonas aeruginosa MutL promotes large chromosomal deletions through non-homologous end joining to prevent bacteriophage predation.

    abstract：:Pseudomonas aeruginosa is an opportunistic pathogen with a relatively large genome, and has been shown to routinely lose genomic fragments during environmental selection. However, the underlying molecular mechanisms that promote chromosomal deletion are still poorly understood. In a recent study, we showed that by del...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky160

    authors： Shen M,Zhang H,Shen W,Zou Z,Lu S,Li G,He X,Agnello M,Shi W,Hu F,Le S

    更新日期：2018-05-18 00:00:00

  • Single-stranded DNA binding protein from human malarial parasite Plasmodium falciparum is encoded in the nucleus and targeted to the apicoplast.

    abstract：:Apicoplast, an essential organelle of human malaria parasite Plasmodium falciparum contains a ∼35 kb circular genome and is a possible target for therapy. Proteins required for the replication and maintenance of the apicoplast DNA are not clearly known. Here we report the presence of single-stranded DNA binding protei...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq565

    authors： Prusty D,Dar A,Priya R,Sharma A,Dana S,Choudhury NR,Rao NS,Dhar SK

    更新日期：2010-11-01 00:00:00

  • Adenovirus DNA replication in vitro: site-directed mutagenesis of the nuclear factor I binding site of the Ad2 origin.

    abstract：:The template requirements for efficient adenovirus DNA replication were studied in vitro in a reconstituted system with cloned DNA fragments, containing the Ad2 origin region, as templates. Replication is enhanced by nuclear factor I, a cellular protein that binds specifically to the Ad2 origin. This stimulation is sh...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/13.13.4935

    authors： de Vries E,van Driel W,Tromp M,van Boom J,van der Vliet PC

    更新日期：1985-07-11 00:00:00

  • AVPpred: collection and prediction of highly effective antiviral peptides.

    abstract：:In the battle against viruses, antiviral peptides (AVPs) had demonstrated the immense potential. Presently, more than 15 peptide-based drugs are in various stages of clinical trials. Emerging and re-emerging viruses further emphasize the efforts to accelerate antiviral drug discovery efforts. Despite, huge importance ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks450

    authors： Thakur N,Qureshi A,Kumar M

    更新日期：2012-07-01 00:00:00